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Anti-cancer Activities of Extract from the Bark of Melia azedarach L. var. japonica Makino  

Kim, Hyun-Woo (Research Institute for Natural Product and Medicines, Semyung University)
Kang, Se-Chan (Research Institute for Natural Product and Medicines, Semyung University)
Publication Information
Korean Journal of Plant Resources / v.22, no.4, 2009 , pp. 312-316 More about this Journal
Abstract
In the present study, the anti-cancer activity of 80% ethanol extracts from 120 kinds of medicinal herbs and native plants were investigated. Among them, the barks of Melia azedarach L. var. japonica Makino showed the highest cytotoxicity in HCT-15 human colon cancer cell. With this result, we carried out hollow fiber (HF) assay and anti-metastasis study to confirm the anti-cancer effects of M. azedarach var. japonica. In MTT assay, M. azedarach var. japonica.inhibited the proliferation of HCT-15 cells in dose-dependent manner. HF assay was carried out using A549 human adenocarcinoma cell, HCT-15 and SK-Hep1 human liver cancer cell via intraperitoneal (IP) and subcutaneous (SC) site. As a results, SK-Hep1 implanted in IP site showed the highest cytotoxicity. The result from metastatic model using B16/BL6 mouse corresponded to that of HF assay. These results suggest that the ethanol extract from M. azedarach var. japonica. might have a potent anti-cancer activity and advanced study is needed for the development of novel natural anti-cancer drug.
Keywords
Anti-cancer; human cancer cell; HF assay; metastasis; Melia azedarach L. var. japonica Makino;
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