• The Korean Journal of Physiology and Pharmacology
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• 제10권4호
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• pp.199-205
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• 2006

The functional expression of potassium $(K^+)$ channels has electrophysiologically been studied in bone cells from several species, however, their identity and regulation of gene expressions in bone cells are not well known. In the present study, to investigate how $K^+$ channel expressions are regulated by estrogen, we measured changes of transcript levels of various $Ca^{2+}$-activated ($K_{Ca}$) and ATP-sensitive $K^+$ channels in rat osteoblastic ROS 17/2.8 cells after treatment with estrogen. Application of 17${\beta}$-estradiol $(E_2)$ for 24 h and 48 h increased mRNA and protein expressions of inwardly rectifying $K^+$ channel (Kir) 6.2 and type 2 small conductance $K_{Ca}$ channel (SK2), respectively. Combined treatment of cells with 17${\beta}-E_2$ and ICI 182,780, a pure antiestrogen, suppressed 17${\beta}-E_2$-induced alterations of SK2 and Kir6.2 mRNA levels. In addition, treatment of cells with U0126, a specific inhibitor of extracellular receptor kinases (ERK)1/2, and SP600125, a specific inhibitor of c-jun N-terminal kinase (JNK) blocked the enhancing effects of 17${\beta}-E_2$ on SK2 and Kir6.2 protein expressions. On the other hand, blocking of p38 mitogen-activated protein kinase had no effect. Taken together, these results indicate that 17${\beta}-E_2$ modulates SK2 and Kir6.2 expressions through the estrogen receptor, involving ERK1/2 and JNK activations.

• The Korean Journal of Physiology and Pharmacology
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• 제2권1호
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• pp.95-100
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• 1998

The conductance change evoked by step depolarization was studied in primarily cultured rat adrenal chromaffin cells using patch-clamp and capacitance measurement techniques. When we applied a depolarizing pulse to a chromaffin cell, the inward calcium current was followed by an outward current and depolarization-induced exocytosis was accompanied by an increase in conductance trace. The slow inward tail current which has the same time course as the conductance change was observed in current recording. The activation of slow tail current was calcium-dependent. Reversal potentials agreed with Nernst equation assuming relative permeability of $Cs^+\;to\;K^+$ is 0.095. The outward current and tail current were blocked by apamin (200 nM) and d-tubocurarine (2 mM). The conductance change was blocked by apamin and did not affect membrane capacitance recording. We confirmed that conductance change after depolarization comes from the activation of the SK channel and can be blocked by application of the SK channel blockers. Consequently, it is necessary to consider blocking of the SK channel during membrane capacitance recording.

• The Korean Journal of Physiology and Pharmacology
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• 제13권5호
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• pp.361-365
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• 2009

Effects of quercetin, a kind of flavonoids, on the vasodilating actions were investigated. Among the mechanisms for quercetin-induced vasodilatation in rat aorta, the involvement with the $Ca^{2+}$ activated $K^+$ ($K_{Ca}$) channel was examined. Pretreatment with NE ($5\;{\mu}M$) or KCl (60 mM) was carried out and then, the modulation by quercetin of the constriction was examined using rat aorta ring strips (3 mm) at $36.5^{\circ}C$. Quercetin (0.1 to $100\;{\mu}M$) relaxed the NE-induced vasoconstrictions in a concentrationdependent manner. NO synthesis (NOS) inhibitor, NG-monomethyl-L-arginine acetate (L-NMMA), at $100\;{\mu}M$ reduced the quercetin ($100\;{\mu}M$)-induced vasodilatation from $97.8{\pm}3.7%$ (n=10) to $78.0{\pm}11.6%$ (n=5, p<0.05). Another NOS inhibitor, L-NG-nitro arginine methyl ester (L-NAME), at $10\;{\mu}M$ also had the similar effect. In the presence of both $100\;{\mu}M$ L-NMMA and $10\;{\mu}M$ indomethacin, the quercetin-induced vasodilatation was further attenuated by $100\;{\mu}M$ tetraethylammonium (TEA, a $K_{Ca}$ channel inhibitor). Also TEA decreased the quercetin-induced vasodilatation in endothelium-denuded rat aorta. Used other $K_{Ca}$ channel inhibitors, the quercetin-induced vasodilatation was attenuated by $0.3\;{\mu}M$ apamin (a SK channel inhibitor), but not by 30 nM charybdotoxin (a BK and IK channel inhibitor). Quercetin caused a concentration-dependent vasodilatation, due to the endotheliumdependent and -independent actions. Also quercetin contributes to the vasodilatation selectively with SK channel on smooth muscle.

• The Korean Journal of Physiology and Pharmacology
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• 제11권5호
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• pp.215-219
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• 2007

Small and large conductance $Ca^{2+}$-activated $K^+(SK_{Ca}\;and\;BK_{Ca})$ channels are implicated in the modulation of neuronal excitability. We investigated how changes in peripheral $K_{Ca}$ channel activity affect mechanical sensitivity as well as the afferent fiber type responsible for $K_{Ca}$ channel-induced mechanical sensitivity. Blockade of $SK_{Ca}$ and $BK_{Ca}$ channels induced a sustained decrease of mechanical threshold which was significantly attenuated by topical application of capsaicin onto afferent fiber and intraplantar injection of 1-ethyl-2-benzimidazolinone. NS1619 selectively attenuated the decrease of mechanical threshold induced by charybdotoxin, but not by apamin. Spontaneous flinching and paw thickness were not significantly different after $K_{Ca}$ channel blockade. These results suggest that mechanical sensitivity can be modulated by $K_{Ca}$ channels on capsaicin-sensitive afferent fibers.

• Journal of Microbiology and Biotechnology
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• 제15권1호
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• pp.80-85
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• 2005

Electrochemical control of the metabolic flux of W. kimchii sk10 on glucose and pyruvate was studied. The growing cell of W. kimchii sk10 produced 87.4 mM lactate, 69.3 mM ethanol, and 4.9mM lactate from 83.1mM glucose under oxidation condition of the anode compartment, but 98.9 mM lactate, 84.3mM ethanol, and 0.2 mM acetate were produced from 90.8 mM glucose under reduction condition of the cathode compartment for 24 h, respectively. The resting cell of W. kimchii sk10 produced 15.9 mM lactate and 15.2 mM acetate from 32.1 mM pyruvate under oxidation condition of the anode compartment, and 71.3 mM lactate and 3.8 mM acetate from 79.8mM pyruvate under reduction condition of the cathode compartment. The redox balance (NADH/$NAD^+$) of metabolites electrochemically produced from pyruvate was 1.05 and 18.76 under oxidation and reduction conditions, respectively. On the basis of these results, we suggest that the neutral red (NR) immobilized in bacterial membrane can function as an electron channel for the electron transfer between electrode and cytoplasm without dissipation of membrane potential, and that the bacterial fermentation of W. kimchii sk10 can be shifted to oxidized or reduced pathways by the electrochemical oxidation or reduction, respectively.

• The Korean Journal of Physiology and Pharmacology
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• 제10권1호
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• pp.19-24
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• 2006

The intermediate conductance $Ca^{2+}-activated$ $K^+$ channels (SK4, IKCa1) are present in lymphocytes, and their membrane expression is upregulated by various immunological stimuli. In this study, the activity of SK4 was compared between Bal-17 and WEHI-231 cell lines which represent mature and immature stages of murine B lymphocytes, respectively. The whole-cell patch clamp with high-$Ca^{2+}$ ($0.8{\mu}M$) KCl pipette solution revealed a voltage-independent $K^+$ current that was blocked by clotrimazole (1 mM), an SK4 blocker. The expression of mRNAs for SK4 was confirmed in both Bal-17 and WEHI-231 cells. The density of clotrimazole-sensitive SK4 current was significantly larger in Bal-17 than WEHI-231 cells ($-11.4{\pm}3.1$ Vs. $-5.7{\pm}1.15$ pA/pF). Also, the chronic stimulation of B cell receptors (BCR) by BCR-ligation (anti-IgM Ab, $3{\mu}g$/ml, 8∼12 h) significantly upregulated the amplitude of clotrimazolesensitive current from $-11.4{\pm}3.1$ to $-53.1{\pm}8.6$ pA/pF in Bal-17 cells. In WEHI-231 cells, the effect of BCR-ligation was significantly small ($-5.7{\pm}1.15$ to $-9.0{\pm}1.00$ pA/pF). The differential expression and regulation by BCR-ligation might reflect functional changes in the maturation of B lymphocytes.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 하계종합학술대회 논문집(1)
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• pp.213-216
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• 2000

In this paper, performance of a CDMA-based packet transmission system is analyzed and simulated in a wireless infrared channel. The indoor wireless infrared channel is modeled as a non-directed diffuse link. The pulse position modulation (PPM) is used as a modulation scheme. From the simulation results, it is shown that the RS coding is very effective in improving the packet-delay characteristics of the CDMA-based packet transmission system. The performance improvement is more significant especially when the infrared channel is in a worse condition.

• 한국방재안전학회논문집
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• 제11권2호
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• pp.45-52
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• 2018

본 연구는 2017년 8월 17일 청계천에서 발생한 시민고립사고의 원인을 규명하고, 고밀도 기상관측망의 관측자료를 이용하여 안전한 도시하천 관리 방안을 제시한 연구이다. SK 텔레콤 기지국에 설치된 고밀도 기상관측망인 SK techx와 상대적으로 공간적 밀도가 낮은 기상청 AWS의 사고 당일 강우자료를 도시유출모형에 적용하여 당시 상황을 모의하였다. 사고원인 중 하나로 가정한 CSO 관로 내 체수현상을 구현하여 수치모의한 결과, 기상청 AWS에서 계측된 강우량은 사고를 발생시키지 않았다. 하지만 실제 현상과 더 유사한 고밀도 기상관측망인 SK techx의 강우자료를 적용했을 때는 당일 발생한 사고와 유사한 결과가 나타났다. 이는 낮은 공간 밀도인 기상청 AWS는 청계천에서 일어나는 실제현상을 예측할 수 없고, 안전한 하천관리르 위해 고밀도 기상관측소가 필요하다는 것을 의미한다. 또한 CSO 관로 내 체수 유무를 독립변수로 수치 모의한 결과 비우당교의 CSO 관로 내 체수가 사고의 직접적인 원인으로 분석되었다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 ITC-CSCC -1
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• pp.109-112
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• 2000

In this paper, performance of multicode DS/CDMA packet radio network with adaptive antenna array is analyzed and simulated in a multipath Rayleigh fading channel. The network performance is evaluated in terms of packet throughput. From the simulation results, it is confirmed that the adaptive antenna array is very effective in improving the packet throughput performance of the multicode DS/CDMA packet radio network. The results of this paper can be applied to design of IMT-2000 or future-generation mobile communication systems.

• 문화기술의 융합
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• 제6권3호
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• pp.427-430
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• 2020

IoT 분야와 의료 측정기기 분야에서 여러 개의 아날로그 입력 신호를 동시에 디지털 신호로 변환하는 기술 요구가 늘어나고 있다. 기존 단일 또는 2개의 체널 방식을 이용하여 여러 개의 아날로그 신호를 처리하는 방식에서는 하드웨어 크기와 전력소모 면에서 응용 제한을 받게 된다. 본 논문에서는 여러 개의 아날로그 입력을 동시에 받아서, 각각에 대한 24비트 디지털 신호를 출력하는 다채널 24비트 ADC 용 콤필터 설계 및 구현을 기술하였다. 제안된 콤필터의 기능은 매트랩 시뮬레이션과 FPGA 테스트 보드로 검증하였다. SK 하이닉스 0,35㎛ CMOS 표준 공정을 이용하여 칩으로 제작하였다. 미분기/적분기 사용 또는 FIR 구조의 기존 방식과 성능, 칩 면적을 비교하였다. 제안된 콤필터는 6개 이상의 다채널 아날로그 입력, 저 전력 소모, 작은 하드웨어 크기를 요구하는 IoT 제품과 의료 측정기기 활용이 예상된다.