• Journal of Life Science
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• 제22권4호
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• pp.437-446
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• 2012

A metagenomic library of cow rumen in the pCC1FOS phage vector was screened in $E.$ $coli$ EPI300 for cellulase activity on carboxymethyl cellulose agar plates. One clone was partially digested with $Sau$3AI, ligated into the $Bam$HI site of the pBluescript II SK+ vector, and transformed into $E.$ $coli$ $DH5{\alpha}$. We obtained a 1.5 kb insert DNA, designated $cel$5C, which hydrolyzes carboxymethyl cellulose. The cel5C gene has an open reading frame (ORF) of 1,125 bp encoding 374 amino acids. It belongs to the glycosyl hydrolase family 5 with the conserved domain LIMEGFNEIN. The molecular mass of the Cel5C protein induced from $E.$ $coli$ $DH5{\alpha}$, as analyzed by CMC SDS-PAGE, appeared to be approximately 42 kDa. The enzyme showed optimum cellulase activity at pH 4.0, and $50^{\circ}C$. We examined whether the $cel$5C gene comes from the 49 identified cow rumen bacteria using PCR. No PCR bands were identified, suggesting that the $cel$5C gene came from the unidentified cow rumen bacteria.

• The Journal of Society for e-Business Studies
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• 제20권4호
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• pp.151-175
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• 2015

Although three types of the information security measures (technical, physical and managerial ones) are all together critical to maintaining information security in the organizations and should be implemented at the same time, this study aims at providing theoretical basis of establishing and implementing effective managerial security measures. The rationale behind this research objective is that it is very important to effectively perform the managerial security measures to achieve the target performance level of the technical and the physical security measures because main agents of practicing the information security measures in the organizations are staff members even though the technical and the physical ones are well constructed and implemented. In particular, this study intends to develop and propose the theoretical model applicable to providing the way of improving organizational members' intention to use information security technologies since the very intention to use them is essential to effectively establishing and promoting managerial security measures. In order to achieve the objective of this study, the factors critical to influencing upon the intention to use information security technologies are derived through systematically reviewing related theories and previous studies, and then the research model and hypotheses are proposed by logically reasoning the casual relationship among the these factors. Also, the empirical analyses are performed by conducting the survey of the organization members of domestic large companies and analyzing the structural equation model by PLS (Partial Least Squares) method. The significant results of this study can contribute to expanding the research area of managerial information security and can be applied to suggesting the practical guidelines for effectively establishing and implementing the managerial security measures in various organizations.

• The Korean Journal of Physiology and Pharmacology
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• 제21권2호
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• pp.259-265
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• 2017

Excessive influx and the subsequent rapid cytosolic elevation of $Ca^{2+}$ in neurons is the major cause to induce hyperexcitability and irreversible cell damage although it is an essential ion for cellular signalings. Therefore, most neurons exhibit several cellular mechanisms to homeostatically regulate cytosolic $Ca^{2+}$ level in normal as well as pathological conditions. Delayed rectifier $K^+$ channels ($I_{DR}$ channels) play a role to suppress membrane excitability by inducing $K^+$ outflow in various conditions, indicating their potential role in preventing pathogenic conditions and cell damage under $Ca^{2+}$-mediated excitotoxic conditions. In the present study, we electrophysiologically evaluated the response of $I_{DR}$ channels to hyperexcitable conditions induced by high $Ca^{2+}$ pretreatment (3.6 mM, for 24 hours) in cultured hippocampal neurons. In results, high $Ca^{2+}$-treatment significantly increased the amplitude of $I_{DR}$ without changes of gating kinetics. Nimodipine but not APV blocked $Ca^{2+}$-induced $I_{DR}$ enhancement, confirming that the change of $I_{DR}$ might be targeted by $Ca^{2+}$ influx through voltage-dependent $Ca^{2+}$ channels (VDCCs) rather than NMDA receptors (NMDARs). The VDCC-mediated $I_{DR}$ enhancement was not affected by either $Ca^{2+}$-induced $Ca^{2+}$ release (CICR) or small conductance $Ca^{2+}$-activated $K^+$ channels (SK channels). Furthermore, PP2 but not H89 completely abolished $I_{DR}$ enhancement under high $Ca^{2+}$ condition, indicating that the activation of Src family tyrosine kinases (SFKs) is required for $Ca^{2+}$-mediated $I_{DR}$ enhancement. Thus, SFKs may be sensitive to excessive $Ca^{2+}$ influx through VDCCs and enhance $I_{DR}$ to activate a neuroprotective mechanism against $Ca^{2+}$-mediated hyperexcitability in neurons.

• Asian Pacific Journal of Cancer Prevention
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• 제17권5호
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• pp.2667-2672
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• 2016

Background: Virtually all cases of cervical cancer are caused by persistent infections with a restricted set of human papillomaviruses (HPV). Cancer of the cervix is the third or even the second most common cancer in women worldwide, more than 85% of the cases occurring in developing countries, such as China and India, including the Republic of Kazakhstan. The purpose was to determine the HPV type distribution to evaluate efficacy of vaccination and adjust cancer prevention strategy in Western Kazakhstan in the future. Materials and Methods: A retrospective analysis was conducted of data obtained from PCR laboratories in 4 regional centers for the time period covering 12 months, 2013-2014, using AmpliSens$^{(R)}$ Real-Time PCR kits for HPV testing of 12 genotypes (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, and 59). Results: A total of 1,661 persons were HPV tested within 2013-14, but a proprotion examined for 16 and 18 genotypes only (563) was not been included for statistic analysis of distribution and ratio of the most common genotypes. Males accounted for only a small number (N=90 in total). Conclusions: Total number of the HPV-positive appeared to be 26.0%, or 286 of N=1098. Types distribution was as follows: type 16 (10.7%), 39 (5.83%), 51 (5.27%), 31 (4.85%), 56 (4.58%), 18 (3.61%), 59 (2.64%), 58 (2.22%), 35 (1.94%), 33 (1.25%). Overall the HPV infection was highest in 16-29 years old (62.4%) and decreased with age. Total prevalence of the HR-HPVs amongst male population was 21.4% with top five types 16, 18, 39, 51, 31. Trends forcorrelations between Aktau site and type 33 (Cramer's V 0.2029), between Caucasian ethnicity and type 33 (Cramer's V .1716), and between European ethnicities in Uralsk and type 45 (Cramer's V .1752) were found. Of N 563 tested separately for 16 or 18 types, 13.6% were positive. As a whole, the distribution of 16/18 types had a ratio of 3.53:1. Given the vaccine-targeted type 16 is widely spread amongst this regional population, HPV immunization program of adolescent girls 10-13 years should be implemented appropriately.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 한국농업기계학회 2017년도 춘계공동학술대회
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• pp.83-83
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• 2017

국내에 유통되는 제초날은 대부분 금속으로 제작되며, 금속 날의 대부분 탄소공구강(SK-3~5)으로 제작되고, 날 수 및 날 형태에 따라 2도 날, 3도 날, 4도 날, 8도 날, 톱날로 구분된다. 대부분 평지 제초작업에 사용되고, 고랑 제초 작업에 알맞은 제초날의 없어 고랑 제초 작업의 효율이 떨어진다. 본 연구는 굴곡이 있는 밭의 고랑 제초 작업에 효율적인 원형 제초날을 설계 하고자 한다. 밭작물의 제초작업은 굴곡이 있는 이랑과 고랑 사이의 잡초를 제거해야 하기 때문에 평지의 잡초를 제거하는 제초날로는 제초작업이 거의 불가능하다. 따라서 고랑 형상에 맞는 원형 제초날을 설계 하였으며, 회전 균형 및 무게의 균일성 설계가 쉬운 뼈대형 원형 제초날을 설계한 후 데이터를 이용하여 원형 제초날을 설계하였다. 또한 고랑의 잡초 뿌리부분을 제거하기 위해 원형 제초날에 돌출부를 추가하는 방법으로 설계하였다. 제초날은 밭작물 고랑폭을 기준으로 하여 높이는 85mm 직경은 300mm로 설계하였으며, 제초날 설계시 원형 제초날은 뼈대형 원형 제초날과 같이 고랑폭을 기준으로 하여 높이 및 직경을 뼈대형 원형 제초날과 동일한 치수로 설계하였다. 원형 제초날 설계에서 돌출부를 볼트 결착방식으로 설계하였으며, 회전중 돌출부의 파손 혹은 이탈을 방지하기 위하여 직경 5mm이상의 볼트 결착이 필요하였다. 볼트 결착방식은 제초날 조립후 제초날 회전시 고랑의 잡초를 효과적으로 제거하기 위한 작업공간 확보가 어려울 것으로 판단되었다. 따라서 제초날 원형부에 돌출부를 절단 절곡하는 방식으로 돌출부를 재설계하였으며, 돌출부 길이는 안쪽 부분 15mm, 바깥 부분 20mm로 설계하고, 돌출부 회전반경은 안쪽 날 20mm, 바깥 날 10mm로 설계하였다. 일반적인 제초날 과 달리 원형 제초날을 개발하기 위해서 회전균형과 안전이 제초날 설계에 중요한 요인이 된다. 뼈대형 제초날을 통하여 제초날의 균형 데이터를 분석하였고 이를 바탕으로 원형 제초날을 설계하였다. 또한 발란싱 머신을 이용한 회전시험에서 unbalance 정도가 3,600rpm에서 4.51/47.1deg로 나타나 제초기에 적용 가능할 것으로 판단되었다.

• Applied Chemistry for Engineering
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• 제9권3호
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• pp.440-444
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• 1998

Poly(phenylene sulfide-co-phenylene sulfide sulfone), PPS/PPSS copolymers were synthesized from p-dichlobenzene(DCB), p-dibromobenzene(DBB), p-diiodobenzene(DIB), 4-chlorophenyl sulfone(CPS) and sodium sulfide as comonomers under high temperature and pressure utilizing N-methyl-2-pyrrolidinone(NMP) as solvent. The yield of PPS/PPSS copolymer shoed maximum at $190^{\circ}C$ with [DBB]/[CPS] and [DIB]/[CPS] comonomer pair, while [DCB]/[CPS] pair exhibited maximum yield at $230^{\circ}C$. The change of yield is in the order of I>Br>Cl as leaving groups were in accordance with nucleophilic aromatic substitution reaction mechanism suggested for the synthesis of PPS type polymers. The molecular weight of PPS/PPSS copolymer was the highest($M_w=8,330g/mol$) with [DBB]/[CPS] comonomers in which [CPS] was 10 mole%. The PPS/PPSS copolymer made with 10 mole% of [CPS] showed about $15^{\circ}C$ higher $T_g$ and $15^{\circ}C$ lower $T_m$ than those of PPS homopolymer, which may be useful from the processing and thermal property point of view. The PPS/PPSS copolymer with 30 mole% of CPS or above did not exhibit Tm. The PPS/PPSS copolymer obtained with comonomer feed ratio of [DBB]/[CPS] = 95/5 mole% under $240^{\circ}C$ showed even higher molecular weight($M_w=10,300g/mole$) than PPS homopolymer made under similar reaction condition, retaining high crystallinity and thermal stability.

• 전자공학회논문지 IE
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• 제44권2호
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• pp.8-13
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• 2007

This paper aims to analyze the characteristics of ITO which are caused by variation of plasma condition to fabricate the OLED of high efficiency. We treated $N_2$ gas and $O_2$ gas plasma on the surface of the ITO by changing their RF plasma power into 100 W, 200 W, 400 W and by changing their 9as pressure into 12 mTorr, 120 mTorr. The work function of ITO that plasma treatment was done by using $N_2$ gas had value of $4.88{\sim}5.07\;eV$, and that by using $O_2$ gas, $4.85{\sim}4.97 eV$. The characteristics of the ITO were most efficient in the $N_2$ gas plasma with the RF power of 200W and gas pressure of 120 mTorr. The rms roughness of ITO surface is the value from AFM image. In this case, ITO obtained $25.2\;{\AA}$ and $30.5\;{\AA}$ in the $N_2$ and $O_2$ gas plasma respectively when it had the RF power of 200 W. But ITO that didn't have plasma treatment was $44.5{\AA}$. The variation of ITO transmittance was almost not discovered by the change of $N_2$ gas and $O_2$ gas pressure.

• The Journal of the Korean dental association
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• 제53권10호
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• pp.688-695
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• 2015

Purpose: The institutionalized elderly have placed the most vulnerable state, and the report about the oral health status of the institutionalized elderly was scarce. The aims of the current study were to investigate the oral health status of institutionalized elderly. Materials and methods: The survey of the oral health status was carried out on 487 institutionalized elderly from 4 longterm care facilities of Seoul metropolitan city, Gyunggi province, and Gangwon province in Korea. Results: The prevalence of dental caries, root caries, and dental calculus of the institutionalized elderly was 19.2%, 15.3%, and 23.7%, respectively. The percentage of edentulism among the institutionalized elderly was 26.2%. Those who had poor oral hygiene and tongue coating were 43.9% and 90.5%, respectively. The percentages wearing complete and removable partial denture on maxilla were 12.8% and 3.3%, respectively. The percentages wearing complete and removable partial denture on mandible were 7.8% and 5.6%, respectively. The percentages of institutionalized elderly needing complete and removable partial denture on maxilla were 29.7% and 27.2%, respectively. The percentages of institutionalized elderly needing complete and removable partial denture on mandible were 24.5% and 30.9%, respectively. Conclusion: The oral health stuatus of institutionalized elderly was poor. For the oral health promotion of elderly in longterm care facilities, it is essential to educate nurses and caregivers about the importance of the oral health and oral hygiene method.

• The Journal of Engineering Geology
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• 제19권1호
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• pp.51-61
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• 2009

Fractures and segmentation in association with the activities of the Yangsan fault are studied around Mt. Namsan, Gyengju city in the southeastern part of Korea. It is believed that the higher values of joint density and fractal dimension with the approach of the center of the Yangsan fault mean intense fracturing due to the fault activity. The boundary between fault damage zone and host rock is inferred to be placed at about 2.7 km from the center of the Yangsan fault where the values of joint density and fractal dimension abruptly decrease and the orientations of joint are also much dispersed. The small faults within the damage zone of the Yangsan fault are definitely divided into right-lateral and left-lateral strike-slip faults. The former is considered to be formed during the right-lateral movement of the Yangsan fault and the latter during the left-lateral movement. The Yangsan fault is segmented in the study area with obvious evidences as follows: (1) the difference of fault strike between northern and southern segments, (2) The geometry of contractional imbricate fans and syncline plunging $9^{\circ}$, $S85^{\circ}E$ at the end of northern segment, and (3) anticline plunging $28^{\circ}$, $N4^{\circ}W$ at the end of southern segment.

• Textile Coloration and Finishing
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• 제27권1호
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• pp.70-79
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• 2015

To establish the optimal conditions for the manufacture of high absortive cellulose film(CF) originated from Styela clava tunic(SCT), the physicochemical properties included absorption was measured in CFs prepared under the various conditions. The highest absorption was observed for SCT-CF20 prepared from the cellulose solution dissolved at $80^{\circ}C$ for 20min, although the filtration treatment did not induce any significance alteration. Also, the absorption was higher in SCT-CF20-F prepared by the freeze drying than SCT-CF20-N(ambient air drying) and SCT-CF20-H(heat drying). The addition of porogen($NaHCO_3$) induced an increase of absorption in SCT-CF20-PF relative to SCT-CF20-F, while the number of interconnected porous structures was enhanced in SCT-CF20-PF. Furthermore, a high level of SK-MEL-2 cells viability was observed in SCT-CF20-PN and SCT-CF20-PF cultured group. These results show that SCT-CF may have high absorption and biocompatibility when prepared from SCT cellulose solution dissolved at $80^{\circ}C$ for 20min after addition of porogen and then subjected to freeze drying.