• Title/Summary/Keyword: SINE-R.C2

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Identification and Phylogenetic Analysis of SINE-R Retroposon Family in cDNA Library of Human Fetal Brain

  • Yi, Joo-Mi;Shin, Kyung-Mi;Lee, Ji-Won;Paik, In-Ho;Jang, Kyung-Lib;Kim, Heui-Soo
    • Animal cells and systems
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    • v.5 no.3
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    • pp.231-236
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    • 2001
  • SINE-R retroposons have been derived from human endogenous retrovirus HERV-K family and found to be hominoid specific. Both SINE-R retroposons and HERV-K family are potentially capable of affecting the expression of closely located genes. From cDNA library of human fetal brain, we identified seven SINE-R retroposons and compared them with sequences derived from GenBank database. The SINE-R retroposons from human feta1 brain showed 85∼97% sequence similarities with the human-specific retroposon SINE-R.C2. They also showed 88∼96% sequence similarities with the sequence of the schizo-cDNA clone that derived from postmortem frontal cortex tissue of a schizophrenic patient. Phylogenetic analysis using the neiqhbor-joining method revealed that the seven new SINE-R retroposons from cDNA library of the human feta1 brain have proliferated independently during human evolution. The data indicate that such SINE-R retroposons are expressed in human fetal brain and deserve further investigation as potential leads to understanding of neuropsychiatric diseases.

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Localization of a Human-Specific Retroposon (SINE-R.C2) to Chromosome 6p21.31 by Radiation Hybrid Mapping

  • Kim, Heui-Soo;Timothy J. Crow
    • Journal of Life Science
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    • v.10 no.2
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    • pp.12-13
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    • 2000
  • A human-specific retroposon SINE-R.C2 has been derived from a human endogenous retrovirus HER V-K 10. It is absent in the genome of nonhuman primates and present within the third intron of the human C2 gene that is located in the class III region of the major histocompatibility complex. In the present study, we determined the regional location of the human C2 gene. The analysis of the Genebridge 4 radiation hybrid mapping panel using PCR amplification located the C2 gene between D6S1422 (10.1 cR) and CHLC.GATA4A03 (21.3) with a lod score of>3.0. This allowed us to localize C2 gene on the human chromosome 6 band p21.31.

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Damage Prediction of Reinforced Concrete Structures due to Ground Motion (지반진동으로 인한 R/C 구조물의 손상에 관한 연구)

  • Rhim, Hong-Chul;Kim, Ji-Yeon
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.6 no.2
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    • pp.195-202
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    • 2002
  • Urbanization and development of industry makes people concerned about quality of circumstances. Problems of vibration are on the rise. Vibration makes inhabitants feel unpleasant and involves structural damage. The purpose of this study is to assess damage of reinforced concrete structures due to ground motions as the parameters of frequency, duration time and aspect ratio of structures are changed. Ground motions were modeled as sine waves. To compare sine waves with real ground motions, two cases are selected; one is blast loading case and the other is earthquake loading. It was intended to provide means to assess R/C structure damage due to ground motions.

Modeling Temperature-Dependent Development and Hatch of Overwintered Eggs of Pseudococcus comstodki (Homoptera:Pseudococcidae) (가루깍지벌레(Pseudococcus comstocki (Kuwana))월동알의 온도발육 및 부화시기예찰모형)

  • Jeon, Heung-Yong;Kim, Dong-Soon;Yiem, Myoung-Soon;Lee, Joon-Ho
    • Korean journal of applied entomology
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    • v.35 no.2
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    • pp.119-125
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    • 1996
  • Temperature-dependent development study for overwintered eggs of Pseudococcus comstocki (Kuwana) wasconducted to develop a forecasting model for egg hatch date. Hatch times of overwintered eggs were comparedat five constant temperatures (10, 15, 20, 25, 27$^{\circ}$C) and different collection dates. A nonlinear, four-parameterdevelopmental model with high temperature inhibition accurately described (R2=0.9948) mean developmentalrates of all temperatures. Variation in developmental times was modeled(~~=0.972w9)it h a cumulative Weibullfunction. Least-squares linear regression (rate=O.O06358[Temp.]-0.07566)d escribed development in the linearregion (15-25$^{\circ}$C) of the development curve. The low development threshold temperature was estimated 11.9"Cand 154.14 degree-days were required for complete development. The linear degree-day model (thermal summation)and rate summation model (Wagner et al. 1985) were validated using field phenology data. In degreedaymodels, mean-minus-base method, sine wave method, and rectangle method were used in estimation of dailythermal units. Mean-minus-base method was 18 to 28d late, sine wave method was 11 to 14d late, rectanglemethod was 3 to 5d late, and rate summation model was 2 to 3d late in predicting 50% hatch of overwinteredeggs. hatch of overwintered eggs.

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Preparation of Paraelectric PLT Thin Films Using Reactive Magnetron Sputtering of Multicomponent Metal Target

  • Kim, H.H.;Sohn, K.S.;Casas, L.M.;Pfeffer, R.L.;Lareau, R.T.
    • Electrical & Electronic Materials
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    • v.11 no.10
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    • pp.53-59
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    • 1998
  • Paraelectric lead landthanum titanate(PLT) thin films have been prepared by a reactive dc magnetron sputtering system using a multicomponent metal target. The surface area control of each element on the target markedly facilitates the fabrication of thin films of complex ceramic compounds. A postdeposition heat-treatment was applied to all as-deposited PLT thin films at annealing temperatures up to 75$0^{\circ}C$ for crystalization. The composition of the PLT(28) thin filmannealed at $650^{\circ}C$ was: Pb, 0.73; La, 0.28; Ti, 0.88; O, 2.9. The dielectric constant and dissipation factor of the thin film(200 nm) at low filed measurements (500 Vcm-1) are 1216 and 0.018, respectively. The charge storage density using a typical Sawyer-Tower circuit with a 500 Hz sine wave was 12.5 $\mu$Ccm-2 at the electric field of 200 kVcm-1.

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Design of a piezovibrocone and calibration chamber

  • Samui, Pijush;Sitharam, T.G.
    • Geomechanics and Engineering
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    • v.2 no.3
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    • pp.177-190
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    • 2010
  • This paper presents the details of indigenous development of the piezovibrocone and calibration chamber. The developed cone has a cylindrical friction sleeve of $150cm^2$ surface area, capped with a $60^{\circ}$ apex angle conical tip of $15cm^2$ cross sectional area. It has a hydraulic shaker, coupled to the cone penetrometer with a linear displacement unit. The hydraulic shaker can produce cyclic load in different types of wave forms (sine, Hover sine, triangular, rectangular and external wave) at a range of frequency 1-10 Hz with maximum amplitude of 10 cm. The piezovibrocone can be driven at the standard rate of 2 cm/sec using a loading unit of 10 ton capacity. The calibration chamber is of size $2m{\times}2m{\times}2m$. The sides of the chamber and the top as well as the bottom portions are rigid. It has a provision to apply confining pressure (to a maximum value of $4kg/cm^2$) through the flexible rubber membrane inlined with the side walls of the calibration chamber. The preliminary static as well as dynamic cone penetration tests have been done sand in the calibration chamber. From the experimental results, an attempt has been made to classify the soil based on friction ratio ($f_R$) and the cone tip resistance ($q_c$).

Correlation Analysis of Binder Stiffness with Deformation Strength and Dynamic Creep Property of Asphalt Mixtures for Evaluation of Kim Test Applicability (바인더 Stiffness와 혼합물의 변형강도 및 동적크리프 특성과의 상관성 분석을 통한 김테스트 적용성 고찰)

  • Kim, Kwang-Woo;Doh, Young-Soo;Cho, Mun-Jin
    • International Journal of Highway Engineering
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    • v.7 no.4 s.26
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    • pp.91-102
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    • 2005
  • This study dealt with correlation analysis of binder stiffness with deformation strength and dynamic creep property of asphalt mixtures for evaluation of applicability of deformation strength $(S_D)$. Two aggregates, with maximum size of 13mm and eight binders were used to produce 16 different mixtures. The stiffness of binder $(G^*/sin\delta)$ was measured using DSR at $64^{\circ}C$. Final deformation(FD) and dynamic stability(DS) were measured by dynamic creep (DC) test, and SD was measured by Kim test for each mixture. Results of correlation analysis between $G^*/sin\delta$, and $S_D$, m and DS showed that correlation with binder stiffness and deformation strength was the highest $(R^2>0.88)$. There was good correlation between DS, FD with $S_D$. The results indicated that rut-resistance property of mixture is better reflected in $S_D$ test than FD or DS of dynamic creep test. Therefore, it is concluded that $S_D$ can be possibly used for evaluation of rut resistance of asphalt concretes with a good reliability if the procedure is standardized.

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SEVERAL RESULTS ASSOCIATED WITH THE RIEMANN ZETA FUNCTION

  • Choi, Junesang
    • Journal of the Chungcheong Mathematical Society
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    • v.22 no.3
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    • pp.467-480
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    • 2009
  • In 1859, Bernhard Riemann, in his epoch-making memoir, extended the Euler zeta function $\zeta$(s) (s > 1; $s{\in}\mathbb{R}$) to the Riemann zeta function $\zeta$(s) ($\Re$(s) > 1; $s{\in}\mathbb{C}$) to investigate the pattern of the primes. Sine the time of Euler and then Riemann, the Riemann zeta function $\zeta$(s) has involved and appeared in a variety of mathematical research subjects as well as the function itself has been being broadly and deeply researched. Among those things, we choose to make a further investigation of the following subjects: Evaluation of $\zeta$(2k) ($k {\in}\mathbb{N}$); Approximate functional equations for $\zeta$(s); Series involving the Riemann zeta function.

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Effect of Epidermal Growth Factor (EGF) on Meiotic Maturation and Pronuclear Formation of Porcine Oocytes Produced In Vitro

  • Song S. H.;Kim J. G.;Song H. J.;Kumar B. Mohana;Cho S. R.;Choe C. Y.;Choi S. H.;Rho G. J.;Choe S. Y.
    • Reproductive and Developmental Biology
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    • v.29 no.2
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    • pp.127-131
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    • 2005
  • The objective of this study was to examine the effect of EGF on meiotic maturation and pronuclear (PN) formation of porcine oocytes. Prepubertal gilt cumulus-oocyte-complexes (COCs) aspirated from $2\~6mm$ follicles of abbatoir ovaries were matured in TCM199 containing 0.1mg/ml cysteine, $0.5{\mu}/ml$ FSH and LH, and EGF (0, 5, 10, 20, 40 ng/ml) for 22 hr at $39^{\circ}C$ in a humidified atmosphere of $5\%$ $CO_2$ in air. They were then cultured for an additional 22hr without hormones. In Experiment 1, to examine the nuclear maturation at 44hr of culture, the expanded cumulus cells were removed by vortexing for 1 min in 3 mg/ml hyaluronidase. The oocytes were fixed in acetic acid: methanol (1:3, v/v) at least for 48 hr and stained with $1\%$ orcein solution for 5 min. Nuclear status was classified as germinal vesicle (GV), germinal vesicle breakdown (GVBD), prophase-metaphase I (PI-MI), and PII-MII under microscope. In Experiment 2, to investigate PN formation, oocytes were fertilized with Percoll-treated freshly ejaculated sperm $(1\times10^5\; cells/ml)$ in mTBM with $0.3\%$ BSA and 2mM caffeine for 5hr, and cultured in NCSU-23 medium with $0.4\%$ BSA. At 6hr of culture, the embryos were fixed in $3.7\%$ formaldehyde for 48hr and stained with 10ug/ml propidium iodide for 30 min. PN status was classified as no or one PN (unfertilized), 2 PN (normal fertilized) and $\geq3$ PN (polyspermy). Differences between groups were analyzed using one-way ANOVA after arc-sine transformation of the proportional data. The rate of oocytes that had reached to PII-MII were significantly (P<0.05) higher in all groups added EGF than that of non-treated group $(67\%)$, but it did not differ among the all added groups $(86\%,\;85\%,\;79\%\;and\;81\%$, in 5, 10, 20 and 40 ng/ml EGF, respectively). No differences on the incidence of 2PN were observed in all treated groups $(25\%,\;30\%,\;33\%,\;29\%\;and\;29\%$, in 0, 5, 10, 20 and 40 ng/ml EGF, respectively), however, in non-treated group, polyspermy tended to be increased ($66\%\;vs\;. 58\%,\;54\%,\;52\%\;and\;55\%$, 0 vs. 5, 10, 20, 40 ng/ml EGF, respectively). These results suggest that EGF can be effectively used as an additive for enhancing oocyte maturation and reducing the incidence of polyspermy in pig.