• Title/Summary/Keyword: SHER

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Polymorphism of the DNA Repair Gene XRCC1 (Arg194Trp) and its role in Colorectal Cancer in Kashmiri Population: a Case Control Study

  • Nissar, Saniya;Sameer, Aga Syed;Rasool, Roohi;Chowdri, Nissar A;Rashid, Fouzia
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.15
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    • pp.6385-6390
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    • 2015
  • Background: Genetic polymorphisms in DNA repair genes may influence individual variation in DNA repair capacity, which may be associated with risk of developing cancer. For colorectal cancer the importance of mutations in mismatch repair genes has been extensively documented. Materials and Methods: In this study we focused on the Arg194Trp polymorphism of the DNA repair gene XRCC1, involved in base excision repair (BER) and its role in colorectal cancer in Kashmiri population. A case-control study was conducted including 100 cases of colorectal cancer, and 100 hospital-based age- and sex-matched healthy controls to examine the role of XRCC1 genetic polymorphisms in the context of colorectal cancer risk for the Kashmiri population. Results: Genotype analysis of XRCC1 Arg194Trp was conducted with a restriction fragment length polymorphism (RFLP) method. The overall association between the XRCC1 polymorphism and the CRC cases was found to be significant (p < 0.05) with both the heterozygous genotype (Arg/Trp) as well as homozygous variant genotype (Trp/Trp) being moderately associated with the elevated risk for CRC [OR=2.01 (95% CI=1.03-3.94) and OR=5.2(95% CI=1.42-19.5)] respectively. Conclusions: Our results suggest an increased risk for CRC in individuals with XRCC1 Arg194Trp polymorphism suggesting BER repair pathway modulates the risk of developing colorectal cancer in the Kashmiri population.

Evaluation of Pheromone Trap Settings for Managing Brinjal Shoot and Fruit Borer (Leucinodes orbonalis) in Brinjal

  • Rahman, Mizanur;Ali, Razzab;Islam, Mohammad Saiful;Wang, Myeong-Hyeon
    • Korean Journal of Plant Resources
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    • v.22 no.3
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    • pp.220-226
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    • 2009
  • An experiment was conducted to find out among 9 trap settings the most appropriate site for trap placement in the Brinjal field based on Brinjal shoot and fruit borer trapping efficiency, shoot and fruit infestation, healthy and total fruit yield, and BCR. The efficiency of different trap setting positions varied significantly. Trapping efficiency observed the $T_{1}$ ensured the minimum shoot and fruit infestation 10.02% and 20.95%, respectively, minimum infested fruit yield (4.75 ton/ha), maximum healthy and total fruit yield (26.72 and 31.47 ton/ha) and the maximum BCR (1.70), which was followed by $T_{2}$ and $T_{4}$. The minimum trapping efficiency of $T_{9}$ treatment led the maximum shoot and fruit infestation 13.89 and 29.26%, respectively, maximum infested fruit yield (7.59 ton/ha), minimum healthy and total fruit yield (17.74 and 25.32 ton/ha) and the minimum BCR (1.00). A correlation between the number of BSFB adults trapped from the most efficient trap setting and the shoot and fruit infestation recorded and found a linear positive correlation between number of BSFB adults trapped and shoot infestation (r = 0.781) and fruit infestation (r = 0.810). The effect of pheromone trap positions observed in this study may be attributed to the easy accessibility of the lures and traps, when they are placed at the canopy.

Explicating morphophysiological and biochemical responses of wheat grown under acidic medium: Insight into to the antioxidant defense and glyoxalase systems

  • Bhuyan, MHM Borhannuddin;Hasanuzzaman, Mirza;Al Mahmud, Jubayer;Hossain, Md. Shahdat;Alam, Mazhar Ul;Fujita, Masayuki
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.236-236
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    • 2017
  • Low soil pH causes from $H^+$ rhizotoxicity results in nutrients unavailability in the growing media, inhibits plant growth, development and reduces crop yields. The present study was carried out to reveal morpholophysiological and biochemical responses of wheat (Triticum aestivum L.) to acidity stress. Four wheat varieties viz. BARI Wheat-21, BARI Wheat-25, BARI Wheat-26 and BARI Wheat-30 were used in the study. Eight-day-old seedlings were exposed to different pH levels (3.5, 4.5, 5.5 and 6.5) of growing media. Acidity stress at any level reduced biomass, water, and chlorophyll contents in all the varieties; whereas BARI Wheat-26 showed the least damage. $H^+$ rhizotoxicity also caused oxidative stress through excess production of reactive oxygen species and methylglyoxal which increase lipid peroxidation in all the varieties but the lowest oxidative damage was observed in BARI Wheat-26 due to better performance of the antioxidant defense and glyoxalase systems. Considering the growth, physiological and biochemical attributes BARI Wheat-26 may be considered as acidity stress tolerant, among the variety examined.

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Development of technical guidelines for hydrologic cycle in urban catchment (도시유역 물순환 해석 기술지침 개발)

  • Kim, Hyeon-Jun;Jang, Cheol-Hee;Noh, Seong-Jin;Cho, Han-Bum
    • Proceedings of the Korea Water Resources Association Conference
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    • 2007.05a
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    • pp.1630-1634
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    • 2007
  • 도시유역에서의 장기간에 대한 물순환 해석 기술은 도시하천의 복원 및 건천화 방지 사업의 점증적인 요구에 따라 필수적으로 확보하여야 하는 기술이지만, 관련 연구와 실무에서의 이해도는 미진하다. 따라서, 사업의 계획과 실무 설계를 위하여 도시유역의 물순환에 대한 이해와 기술을 지원할 수 있는 체계적인 지침이 필요하다. 외국의 사례를 살펴보면 일본의 경우 1995년 건설성의 주요 정책으로 "도시에서 적정한 물순환계 재생에의 노력"을 추진하였으며, "도시의 물순환 재생 구상 책정 매뉴얼"을 작성하여 사례 연구로 6개 유역을 대상으로 검토하였고, 2000년에는 "도시유역 물순환계의 정량화 방안"을 우수저류침투기술협회에서 주관하여 물순환계 재생구상 또는 물순환계 마스터플랜을 수립함에 있어서 필요로 하는 물순환계의 해석적인 평가방법의 해설서를 발간하였다. 호주의 경우는 1999년부터 WSUD(Water Sensitive Urban Design)란 개념을 도입하여, 정부 및 각 지방별로 프로그램을 진행하고 있으며, 지침서와 기술지도서를 작성하여 교육을 하고 실무에 활용하고 있고, 사례 연구와 학술회의를 통하여 기술 교류를 활발히 하고 있다. 자연적인 요소와 인공적인 요소가 복잡하게 조합되어있는 도시지역의 물순환의 상태를 파악하기 위해서는 유역의 자연특성과 사회특성 등에 관한 기초 자료의 수집이 선행되어야 하며, 다양한 관측결과를 기초로 물 순환계의 구조, 인과관계를 알기 위해, 또 물순환계 구성요소의 일부가 변화한 경우 다른 부분에 미치는 영향을 정량적으로 평가하기 위해서는 모델링이 필요하다. 또한 이 같은 해석 모형을 이용하여 여러 가지 물순환 개선 정책의 효과를 평가하고 그 결과를 시각적으로 나타내는 것이 가능하다면 정책의 입안에 관계하는 사람들에 있어 공통의 의사결정 지원 도구가 될 것이다. 따라서, 본 연구에서는 도시유역의 물순환 해석을 위한 일련의 과정, 즉 자료의 조사 및 취득에서부터 물순환 해석 모형을 이용한 정량적 현황파악, 물순환 개선 기법 및 평가를 수행함에 있어 주요 착안점 및 실무에서의 기술적 가이드를 제공하고자 하였으며, 보다 세밀한 도시유역의 물순환 해석을 위하여 우리나라와 일본에서 적용이 활발한 물리적 기반의 분포형 모형(WEP, SHER, SWMM)의 적용사례를 통하여 국내 도시하천의 물순환 해석에 활용함에 있어서의 실질적인 적용절차 등을 제시하고자 하였다.

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Effects of Tannery Wastes on the Fattening of Growing Cattle, Carcass, and Meat Quality

  • Alam, Jahangir;Hossain, Mufazzal;Beg, Anwarul Haque;Nam, Ki-Chang;Lee, Sang-Suk
    • Food Science of Animal Resources
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    • v.30 no.2
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    • pp.190-197
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    • 2010
  • The present study was conducted to determine the effect of tannery waste protein concentrate (TWPC) on fattening of cattle and the carcass and meat quality, with the aim of replacing the costly commercial protein concentrate (Jasoprot) with a more economical and effective alternative. Twelve young cattle (six male and six female) were fed during the study period on a control diet (T1) with 10% Jasoprot and on two test diets: 5% TWPC + 5% Jasoprot (T2) and 10% TWPC (T3). The test diets significantly affected (p<0.05) live weight gain and profitability compared to the control diet, perhaps due to the increased protein and essential amino acid content, relative to Jasoprot. TWPC was free of aflatoxin. Sensory-evaluated organoleptic scores did not differ among the groups. Chemical composition was normal as other beef and was non toxic especially within recommended chromium level ($1.90{\pm}0.6{\mu}g$) Total lipid contents were higher (p<0.05) in T3, and moisture, ash and crude protein contents were almost similar (p>0.05) among the three groups. It is concluded that TWPC or an equal mixture of TWPC and Jasoprot may be an economic and efficient alternative protein source to Jasoprot in the cattle industry, which minimizes adverse effects on carcass and sensory meat quality.

Spinoside, New Coumaroyl Flavone Glycoside from Amaranthus spinosus

  • Azhar-ul-Haq,;Malik, Abdul;Khan, Anwar-ul-Haq Sher Bahadar;Shah, Muhammad Raza;Muhammad, Pir
    • Archives of Pharmacal Research
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    • v.27 no.12
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    • pp.1216-1219
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    • 2004
  • Spinoside, new coumaroyl flavone glycoside was isolated from the n-butanol fraction of the mathanolic extract of the whole plant of Amaranthus spinosus and assigned the structure 7-pcoumaroyl apigenin 4-O-${\beta}$ -D-glucopyranoside (1) on the basis of spectroscopic techniques including 1D and 2D NMR spectroscopy. In addition ${\alpha}$ - xylofuranosyl uracil (2), ${\beta}$ -D-ribofuranosyl adenine (3) and ${\beta}$ -sitosterol glucoside (4) have also been isolated for the first time from this species.

Enterohepatic Recycling of Estrogen and its Relevance with Female Fertility

  • Sher, Alam;Rahman, M.Ataur
    • Archives of Pharmacal Research
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    • v.23 no.5
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    • pp.513-517
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    • 2000
  • Enterohepatic recycling of estrogen after oral administration of 1 mg non-radioactive estriol was studied in fourteen women selected as the control subjects and ten infertile women in whom the infertility was appearing to be of endocrine origin. The extent of enterohepatic recycling of estriol ($E_3$) during the early follicular phase of menstrual cycle was assessed by monitoring during 48 h the urinary excretion of its two major metabolites i.e; estriol 16 $\alpha$-glucuronide ($E_3-16$$\alpha-G$) and estriol-3 glucuronide ($E_3$-3-G). The change in urinary level of $E_3$-3-G with respect to ($E_3-16$$\alpha-G$G was considered to reflect the extent of enterohepatic recycling of estriol. Lower values of urinary output of both metabolites in the infertile women as compared with the control subjects and the urinary excretion profile of both metabolites during 48 h after estriol ingestion reveal that the reduced extent of enterohepatic recycling could possibly be one of the factors which contribute towards the incidence of infertility in women.

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Heterojunction of FeOOH and TiO2 for the Formation of Visible Light Photocatalyst

  • Rawal, Sher Bahadur;Chakraborty, Ashok Kumar;Lee, Wan-In
    • Bulletin of the Korean Chemical Society
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    • v.30 no.11
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    • pp.2613-2616
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    • 2009
  • FeOOH/$TiO_2$, a heterojunction structure between FeOOH and $TiO_2$, was prepared by covering the surface of the $\sim$100-nm-sized FeOOH particles with Degussa P25 by applying maleic acid as an organic linker. Under visible light irradiation (${\lambda}{\geq}$ 420 nm), FeOOH/$TiO_2$ showed a notable photocatalytic activity in removal of gaseous 2-propanol and evolution of $CO_2$. It was found that FeOOH reveals a profound absorption in the spectral range of 400 - 550 nm, and its valence band (VB) level is located relatively lower than that of $TiO_2$. The considerable photocatalytic efficiency of the FeOOH/$TiO_2$ under visible light irradiation was therefore deduced to be caused by the hole transfer between the VB of FeOOH and $TiO_2$.

Transient and stable expression of hepatitis B surface antigen in tomato (Lycopersicon esculentum L.)

  • Srinivas, L.;Sunil Kumar, G.B.;Ganapathi, T.R.;Revathi, C.J.;Bapat, V.A.
    • Plant Biotechnology Reports
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    • v.2 no.1
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    • pp.1-6
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    • 2008
  • Cotyledonary leaves of tomato cv. Megha were transformed with the hepatitis B virus 's' gene, which encodes surface antigen. Six plant expression cassettes (pHBS, pHER, pEFEHBS, pEFEHER, pSHER and pEFESHER) were used to assay the possible expression levels by agroinfiltration. The maximum transient expression level of 489.5 ng/g D.W. was noted in pEFEHER-infiltrated cotyledonary leaves. Transgenic tomato plants with pEFEHBS and pEFEHER expression cassettes were regenerated and characterized by molecular analysis. The expression of the antigen in the fruits was confirmed by RT-PCR and ELISA analysis. This is the first report on the expression of hepatitis B surface antigen in tomato.

Sequencing and annotation of the complete mitochondrial genome of a threatened labeonine fish, Cirrhinus reba

  • Islam, Mohammad Nazrul;Sultana, Shirin;Alam, Md. Jobaidul
    • Genomics & Informatics
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    • v.18 no.3
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    • pp.32.1-32.7
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    • 2020
  • The mitochondrial genome of a species is an essential resource for its effective conservation and phylogenetic studies. In this article, we present sequencing and characterization of the complete mitochondrial genome of a threatened labeonine fish, Cirrhinus reba collected from Khulna region of Bangladesh. The complete mitochondrial genome was 16,597 bp in size, which formed a circular double-stranded DNA molecule containing a total of 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes) with two non-coding regions, an origin of light strand replication (OL) and a displacement loop (D-loop), similar structure with other fishes of Teleostei. The phylogenetic tree demonstrated its close relationship with labeonine fishes. The complete mitogenome of Cirrhinus reba (GenBank no. MN862482) showed 99.96% identity to another haplotype of Cirrhinus reba (AP013325), followed by 90.18% identity with Labeo bata (AP011198).