• Journal of Microbiology and Biotechnology
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• v.14 no.4
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• pp.707-714
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• 2004

How to improve the cell culture method on scaffolds is important in the tissue engineering fileld. In this study, we optimized seeding and culture methods to vascular smooth muscle cells (VSMCs) on biodegradable polymer scaffold. The primary culture of VSMCs obtained from canine external jugular vein was accomplished by applying the explant-derived method. The primary cultured VSMCs were seeded into scaffolds and then cultured by using various different methods; static or dynamic seeding, static or dynamic culture. The difference in proliferative response of VSMCs was analyzed with an alamar blue assay. Cell-polymer construct was examined by histochemical method and scanning electron microscopy. Mesh type scaffold ($10 \times 10 \times0.4 mm$) was made of polyglycolic acid (PGA) suture thread. The PGA mesh type scaffold was 45% in porosity, and 0.03 g in weight. The primary cultured VSMCs were confirmed with immunohistochemical staining using monoclonal anti-$\alpha$-smooth muscle actin. The density and distribution of proliferated VSMCs within the scaffold and cellular adherence on the surface of the scaffold showed better results in the static seeding condition than in the dynamic condition. Under the same condition of seeding method as the static condition, the dynamic culture condition showed enhanced proliferation rates of the VSMCs when compared to the static culture condition. In conclusion, to improve the VSMCs proliferation in vitro, static seeding is better than the dynamic condition. In the culture condition, however, culture under the dynamic status is better than the static condition. This was a pilot study to manufacture artificial vascular vessel by tissue engineering.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.47 no.5
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• pp.15-22
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• 2015

The crop production technologies keep in improving and the cultivation becomes more standardization owing to the significant developments of various agricultural materials. The artificial soil and base system for root could be one of the major technologies for the modern cultivation especially for controlled horticulture. Although the perlite, cocopeat, and peat moss are the major components of the artificial soil and are broadly used for various application, there is a great need for the new alternative materials for overcoming the low nutrition and the possible shortage of raw materials. In this study, the application of oil palm EFB fiber as an alternative materials for artificial soil especially for the seeding pad components was evaluated. The changes in the structural properties and the functional properties such as moisture holding properties were compared by laboratory produced seeding pads with different mixture of oil palm EFB fiber. The addition of fibrillated EFB fiber resulted in the significant increase in durability of the seeding pad, which showed the possible application of EFB fiber to the seeding pad instead of the wood fiber (UBKP). The moisture holding properties and the germination condition characteristics of the EFB fiber showed the slight less than those of the cocopeat, which require more sophisticated study for improving the functional properties of seeding pad made of the EFB fiber.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.4
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• pp.409-415
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• 1984

Finding the variations of principal agronomic traits, fourteen malting barley cultivars were tested with both narrow-spaced row seeding and broadcasting on high ridge respectively. The seeding rates were varied as 15, 20, 25, 30 and 35 liter per 10a in the narrow-spaced row seeding and 20, 25, 30, 35, and 40 litre in the broad casting on his ridge respectively. As a result of the test, there were highly significant differences in variation pattern of assessed traits among cultivars. However, the additional seeding rates in both seeding methods caused the drifts of earlier heading and maturity, longer in culm length, linear increase in number of spike and yields per unit area, slight reduction in 1000 grain weight, and slight increase in one litre weight, but no changes in number of grain per spike.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.3
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• pp.195-202
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• 1993

This experiment was carried out to investigate the eft'ects of different seeding dates on the agronomic characteristics, forage yield and quality of fall sown cats(Avena sativa L.). The experiment was conducted at the forage experimental field, Seoul National University. Suweon in 1990. The results obtained are summarized as follows; 1. Present data indicated that plant and ear heights were decreased as the seeding date was delayed. Dry matter accumulation was increased due to the higher plant and ear heights. 1. When oats was seeded on August 20. the first heading and 50% heading dates were recorded on October 4 and 15, respectively. And when oats was seeded on August 25. the first heading and 5 0'!4 heading dates were observed on October 13 and 18. respectively. As seeding date was delayed for one day. the heading date was delayed for two days. 3. The dry mattcr percentage of oats seeded on August 20 was 20.1%, but that of oats seeded after August 10 was 15.1 to 16.7%. 4. According to the contents of CP. ADF and NDF, oats seeded on August 20 and 25 was evaluated to be the 3rd or 4th grade hay. and the other oats sceded after August 15 was recorded the 2nd or 3rd grade hay as proposed by AFGC. 5. There are no significant dry matter yield differences among the seeding dates of oats such as August 20, 25 and 30. but a significant yield difference was recorded between oats seeded in August and September. The same trend as the dry matter yield was observed in in vitro digestible dry matter and crude protein yields of oats seeded in August and September. According to the results. early seeding of early matuaring oats may be a better option than late seeding after August 30 for silage or hay production following silage corn harvest in the middle northern area of Korea.

• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.8-14
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• 2005

This study was carried out to investigate the possibility of double cropping of safflower(Carthamus tinctotious L.) in vinyl house for rainshield culture. Planting methods, i.e., direct seeding as the first crop and direct seeding and transplanting as the second crop, were investigated. Growth characters and establishment were better in the first crop than in the second crop. Between direct seeding and transplanting of the second crop, growth was not significantly different, but establishment was much higher in direct seeding than in transplanting. Seed yield of the second crop was lower $30\~74\%$ than that of the first crop. In planting methods of the second crop, seed yield was higher in direct seeding than in transplanting. Therefore, optimum method of the second crop for double cropping of safflower in the middle parts of Korea was estimated as direct seeding.

• Korean Journal of Medicinal Crop Science
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• v.13 no.6
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• pp.241-244
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• 2005

This study was carried out to compare growth characteristics, and extract and crude saponin contents of 4-year-old ginseng cultured by direct seeding and transplanting cultivation at several farms of the main producing district, Geumsan and Eumsung. Though root weight per plant of direct seeding cultivation was lower than that of transplanting cultivation, but yield of the former was higher than that of the latter owing to high rate of survival plant and Leaf Area Index. Dry matter partitioning ratio of direct seeding cultivation was high in primary root and low in secondary root because direct seeding cultivation elongated the length of primary root, while it suppressed the growth of secondary root. Ratio of rusty root was decreased in condition of direct seeding cultivation, while the contents of extract and crude saponin were lower than that of transplanting cultivation.

• Journal of Crop Science and Biotechnology
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• v.11 no.3
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• pp.193-198
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• 2008

To develop a natural re-seeding technology, the optimum soil incorporation time of Chinese milk vetch(CMV) plant residues, seed persistence in soil, biomass production and subsequent rice yield were determined from 2005 to 2007 in rice with CMV green manure in southern Korea. Generally, insufficient seedling stand was regenerated with CMV incorporation to the soil at 20 and 25 days after flowering(DAF), while high regeneration of greater than 400 seedlings per $m^2$ was observed at 35 and 40 DAF. High re-seeding stand of CMV incorporated at 35 DAF or later was related with high seed viability and heavy seed weight. Appreciable number of CMV seeds remained 4 month after burial in soil and a good number of CMV seedlings regenerated from this seed bank at rice harvest time in the fall. Based on the relationships among re-seeding number of CMV plants, seed weight and seed viability, CMV plants should be incorporated into the soil 35 DAF(May 30) or later when CMV seeds were sufficiently matured. The natural re-seeding stand for the 3-year trials was stable ranging from 437 to 700 plants per $m^2$ and the biomass production was sufficient to supply nitrogen for rice growth. The use of re-seeding CMV plant can produce similar rice yield like that of rice without CMV green manure.

• Journal of Biosystems Engineering
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• v.34 no.2
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• pp.106-113
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• 2009

This study was carried out to test feasibility of a granule fertilizer applicator for rapeseed seeding in paddy fields. The metering plate of a conventional applicator was modified for rapeseed seeding. The modified plate had new three seeding openings with total area of $342mm^2$. Average target discharging rates ranged from 6.3 to 21.0 g/s and correlated with opening areas. Application patterns of the rapeseed seeding showed M-shape. The coefficients of variation of the application showed 70.5% at 0.99 m/s of forwarding speed and 14.3 g/s of a discharge rate, and 77.7% at 1.72 m/s of a forwarding speed and 8.23 g/s of a discharge rate. The coefficients of variation in the direction of transverse of the tractor ranged from 7.3 to 47.7%. In rapeseed seeding tests using the applicator, seed damages rates were less than 15% due to the agitator, the seed hopper and the seeding plate break. Therefore, improvements of the applicator blow heads and seeding equipments were required in order to apply rapeseed efficiently.

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.174-177
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• 2011

Green manure crops play an important role in organic farming. Field experiment was conducted at paddy soil (fine loamy, mixed, nonacid, mesic family of Aeric Fluvaquentic Endoquepts) in 2008/2009 to 2009/2010 at the National Institute of Crop Science (NICS), RDA, Suwon, Gyeonggi province, Korea. This experiment was carried out to evaluate the biomass of hairy vetch (Vicia villosa) and growth of rice (Oryza sativa) by different seeding rates. Seeding rates of hairy vetch consisted of 30, 60, and 90 kg $ha^{-1}$ by broadcasting before rice harvesting. The biomass and nitrogen production of hairy vetch were not significantly different between 60 kg $ha^{-1}$ and 90 kg $ha^{-1}$ of seedinq rates. Also, rice yield was not significantly different between seeding rate 60 kg $ha^{-1}$ of hairy vetch and conventional practice for two years. Therefore, we suggested that seeding rate of hairy vetch should be reduced by continuous cropping and incorporation of hairy vetch under rice-based cropping system.

• Journal of The Korean Society of Grassland and Forage Science
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• v.10 no.2
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• pp.65-69
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• 1990

An experiment was carried out ot determine the effects of seeding rates and seedbed preparations on the establishment and yield of surface-sown pasture species. Establishment of orchardgrass was significantly increased by the lowest seeding rate of 20 kg/ha as compared to 30 and 40 kg/ha, but no differences were found between the seeding rates of ladino clover. Both raking and burning significantly improved the establishment of pasture species in comparison with non-treatment. There was a significant difference between the raking and burning for the establishment of orchardgrass, but no significant difference was found between the two treatments for that of ladino clover. Increasing seeding rate of pasture species did not enhance the yield of surface-sown pasture, but both the raking and non-treatment significantly increased the yield of pasture species. The results suggest that both the raking and burning can improve the establishment but increasing seeding rate over 20 kg/ha and burning may not increase the yield.