• Title/Summary/Keyword: SEED MORPHOLOGY

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Seed extracts of a Bangladeshi medicinal plant Abrus precatorius L. show antifertility activity in female rats

  • Hannan, M.A.;Hossain, M.A.;Islam, M.T.
    • Advances in Traditional Medicine
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    • v.10 no.2
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    • pp.103-110
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    • 2010
  • The seed powder of Abrus precatorius L. has traditionally been used as oral contraceptive agent by the women in some rural areas in Bangladesh. The present study aimed to examine the antifertility activity of A. precatorius seed extracts in experimental female rats. Finely ground seeds were extracted with aqueous acetone followed by successive partitioning with n-hexane, ethyl acetate (EtOAc), methanol (MeOH) and water. Water suspended crude seed powder, organic fractions of acetone extract and a standard contraceptive drug ($Nordette^{(R)}28$) were separately administered orally to the female rats for 30 days. n-Hexane, EtOAc and MeOH solubles at the doses of 2, 4 and 6 mg/rat/day, respectively and crude seed powder at 100 mg/rat/day exhibited 100% antifertility activity with lowest levels of serum luteinizing hormone (LH), follicle stimulating hormone (FSH) and $17{\beta}$-estradiol. Histological study of ovary and uterus of these rats exhibited reduced number of developing follicles and increased number of atretic follicles in the ovary, and fewer uterine glands with shrunken morphology, reduced endometrial height, poor vascularity and compact stroma in uterus. However, the activities of serum glutamate oxaloacetate transaminase and serum glutamate pyruvate transaminase and the body weight of the rats remained almost unaffected in all the seed extract treated rats compared to control. These results suggest that A. precatorius seed extracts reduced the levels of serum FSH, LH and $17{\beta}$-estradiol probably by affecting hypothalamic-pituitary-gonadal axis. The reduced levels of these hormones might have affected the oestrous cycle, follicular development, and subsequently the establishment of pregnancy in treated rats.

Seed Germination and Dormancy Breaking of Thalictrum rochebrunianum var. grandisepalum (H. Lev.) Nakai

  • Cho, Ju Sung;Kwon, Hyuk Joon;Lee, Cheol Hee
    • Korean Journal of Plant Resources
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    • v.29 no.3
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    • pp.339-346
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    • 2016
  • This study was carried out to develop an effective seed propagation method for Thalictrum rochebrunianum var. grandisepalum (H. Lev.) Nakai by analyzing seed dormancy types and germination characteristics. Seeds were collected between September to October at Gangwon province, and well-selected seeds were used while being dry-stored at 4±1℃. The seed size ranged 4.52 × 1.58 ㎜ and the weight of thousand seeds were 1,603.5 ± 0.02 ㎎. The moisture content was 7.2%. Seeds were achene type, and morphology characters showed an elliptical shape and rough texture, and light brown in color. Moist-chilling treatment was conducted for dormancy breaking because the seeds had an undeveloped embryo of liner type. The embryo had developed during a moist-chilling period, constantly, and fully developed in 10 weeks. Consequently, it seemed to be non-deep complex or intermediate complex type of morphophysiological dormancy, and embryo dormancy was broken by wet-chilling for 10 weeks. After 10 weeks of wet-chilling treatment, seed germination began. Germination percentage was higher in dark condition raher than light condition and recorded the maximum at 25℃ in the dark (16.3%). A pre-soaking treatment with a combined plant growth hormones promoted germination and shortened T50. Specifically, seed germination of 84.5% was achieved by pre-soaking of seeds with a combined solution of 500 ㎎/L GA3 and 10 ㎎/L kinetin for 24 h after a wet-chilling treatment for 10 weeks. Thus the effect of plant growth hormones coupled with chilling temperature on seed breaking dormancy provide asubsequent growth of seedlings for successful plantation.

Effect of Seed Size and Mucilaginous Substance on Seed Germination of Peony(Paeonia lactiflora Pall.) (작약(芍藥) 종자(種子)의 형태적(形態的) 특성(特性)과 점액물(粘液物)이 발아(發芽)에 미치는 영향(影響))

  • Jeong, Yeun Seon;Sohn, Jae Keun
    • Current Research on Agriculture and Life Sciences
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    • v.13
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    • pp.65-70
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    • 1995
  • The experiments were conducted to determine the influence of seed size and mucilaginous substance on seed germination of herbaceous peony collected from farmers' fields in Korea. No significant difference was recognized between the size of seed and the rale of germination. The maximum percentage(68%) of seed germination was obtained from the seeds with thin brown colour treated at $20^{\circ}C$. The amount of mucilaginous substances exuded from peony seeds was the highest in the seeds with black brown colour. Germination rate of peony seeds was negatively correlated with the amount of mucilaginous substance exuded from the seeds. High frequency(93%) of seed germination was obtained from the seeds which treated in the mixture(1:1) of sands and activated charcoal.

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Hydrothermal Synthesis of Rod-like Copper Oxide Crystals

  • Pee, Jae-Hwan;Lee, Dong-Wook;Kim, Ung-Soo;Choi, Eui-Seok
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 2006.09a
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    • pp.167-168
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    • 2006
  • A hyrdrothermal synthesis has been developd to prepare rod-like crystals of copper oxide using copper nitrate trihydrate as a function of synthesis temperature, stirring speed and solution pH value. The properties of the fabricated crystals were studied using scanning electron microscopy, X-ray diffraction and particle size analysis. The morphology of the synthesized CuO was dependent on both the pH value of the solution and the morphology of the seed materials. Synthesized particles have regular morphologies and a uniform size distribution.

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Morphology and distribution of antennal, maxillary palp and labial palp sensilla of the adult bruchid beetles, Callosobruchus chinensis (L.) (Coleoptera: Bruchidae)

  • WANG, Hongmin;ZHENG, Haixia;ZHANG, Yaowen;ZHANG, Xianhong
    • Entomological Research
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    • v.48 no.6
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    • pp.466-479
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    • 2018
  • Callosobruchus chinensis (Coleoptera: Bruchidae) is the main pest of mungbean (Vigna radiata) especially during seed storage. To provide background information for chemical ecology studies, we examined the externalmorphology of the antennal, maxillary palp and labial palp sensilla of this insect with scanning electron microscopy. The antennae of adult C. chinensis exhibited sexual dimorphism. The female have serrated antenna and the male have pectinate morphology. Eight sensillum types have been observed on the antennae of both sexes: $B\ddot{o}hm$ bristles, sensilla chaetica, sensilla trichodea, sensilla basiconica, sensilla styloconicum, sensilla coeloconica, sensillum campaniformia, sensilla cavity. There are no differences on the morphology of maxillary palp and labial palp between female and male. Four different types of sensilla are present on the labial and maxillary palps of both sexes of C. chinensis: sensilla chaetica, sensilla twig basiconica, sensilla basiconica, sensilla digitiformia.

Contour detection of hippocampus using Dynamic Contour Model and Region Growing (영역확장법과 동적외곽선모델을 이용한 해마(hippocampus)의 외곽선 검출)

  • Jang, D.P.;Kim, H.D.;Lee, D.S.;Kim, S.I.
    • Proceedings of the KOSOMBE Conference
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    • v.1997 no.05
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    • pp.116-118
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    • 1997
  • In hippocampal morphology Abnormalities, including unilateral or bilateral volume loss, are known to occur in epilepsy, Alzheimer's disease, and in certain amnestic syndromes. To detect such abnormalities in hippocampal morphology, we present a method that combines region growing and dynamic contour model to detect hippocampus from MRI brain data. The segmentation process is performed two steps. First region growing with a seed point is performed in the region of hippocampus and the initial contour of dynamic contour model is obtained. Second, the initial contour is modified on the basis of criteria that integrate energy with contour smoothness and the image gradient along the contour. As a result, this method improves fairly sensitivity to the choice of the initial seed point, which is often seen by conventional contour model. The power and practicality of this method have been tested on two brain datasets. Thus, we have developed an effective algorithm to extract hippocampus from MRI brain data.

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Antimicrobial Effect of Grapefruit Seed Extract on Vibrio parahaemolyticus Isolated from the Southern Adjacent Sea of Korea (남해안연안에 분포되어 있는 Vibrio Parahaemolyticus의 성상 및 Grapefruit Seed Extract 처리에 의한 향균효과)

  • 조성환;강동훈;전상수;정덕화
    • Journal of Food Hygiene and Safety
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    • v.9 no.3
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    • pp.141-149
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    • 1994
  • To investigate the distribution of Vibrio parahaemopyticus in the southern 4 coastal areas, Vibrio parahaemolyticus was isolated from seawater, shellfish and sediment from May to October in 1991, and antimicrobial effect of grapefruit seed extract (GFSE) on the growth of isolated strains were examined. In the 120 sample from 4 coastal areas, 16 strains of Vibrio parahaemolyticus were isolated and identified. The distribution serotype of isolated strains was 10 types of monovalent k-antiserum. Especially k-5 and k-28 were highly distribyted with 3 and 4 strains. 31.3% of isolated strains showed positive on Kanagawa phenomenon test. All isolated Vibrio parahaemolyticus were sensitive to chloramphenicol and gentamycin, 5 and 6 strains were resistant to streptomycin and colistin, respectively. Isolated strains were compared with geographical, month and sample. The distribution of 16 isolated Vibrio parahamolyticus was high at Hadong with 50%(8 strains), on July with 43.8%(7 strains) and from seawater with 37.5%(6 strains) respectively. Minimal inhibitory level of GFSE to Vibrio parahaemolyticus was 50 ppm. With 100 ppm treatment of GFSE, the destroy of cell membrane function, outflow of cell ingredients and ghost morphology of cell were investigated.

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Control of Grain Size of PZT Thin Film through Seed Layers (Seed Layer를 통한 PZT 박막의 결정립 크기 조절)

  • Kim, Tae-Ho;Kim, Ji-Young;Lee, In-Sup
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2000.05b
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    • pp.273-278
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    • 2000
  • In order to study effects of interface layers between PZT films and electrodes for MFM(Metal-Ferroelectric-Metal) structure capacitors, we have fabricated the capacitors with the Pt/PZT/interface-layer/Pt/$TiO_2/SiO_2/Si$ structure. $PT(PbTiO_3)$ interface layers were formed by sol-gel deposition and PbO, $ZrO_2$ and $TiO_2$ thin layers were deposited by reactive sputtering. $TiO_2$ interface layers result in the finest grains of PZT films compared to $PbO_2$ and $ZrO_2$ layers. On the other hand, PT interface layers result in improved morphology of PZT films and do not significantly change ferroelectric properties. It is also observed that seed layers at the middle and top of PZT films do not give significant effects on grain size but the PT seed layer at the interface between the bottom electrode and the PZT films results in the small grain size.

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Novel Environmentally Benign and Low-Cost Pd-free Electroless Plating Method Using Ag Nanosol as an Activator

  • Kim, Jun Hong;Oh, Joo Young;Song, Shin Ae;Kim, Kiyoung;Lim, Sung Nam
    • Journal of Electrochemical Science and Technology
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    • v.8 no.3
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    • pp.215-221
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    • 2017
  • The electroless plating process largely consists of substrate cleaning, seed formation (activator formation), and electroless plating. The most widely used activator in the seed formation step is Pd, and Sn ions are used to facilitate the formation of this Pd seed layer. This is problematic because the Sn ions interfere with the reduction of Cu ions during electroless plating; thus, the Sn ions must be removed by a hydrochloric acid cleaning process. This method is also expensive due to the use of Pd. In this study, Cu electroless plating was performed by forming a seed layer using a silver nanosol instead of Pd and Sn. The effects of the Ag nanosol concentration in the pretreatment solution and the pretreatment time on the thickness and surface morphology of the Cu layer were investigated. The degrees of adhesion to the substrate were similar for the electroless-plated Cu layers formed by conventional Pd activation and those formed by the Ag nanosol.

Growth Characteristics of Salix nipponica (선버들의 생장 특성)

  • Lee, Pal-Hong;Son, Sung-Gon;Kim, Cheol-Soo;Oh, Kyung-hwan
    • Journal of Wetlands Research
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    • v.4 no.2
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    • pp.1-11
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    • 2002
  • The morphology, seed germination, and life history of Salix nipponica were investigated in the wetland of the Nam-River, Jinju, Gyeongsangnam-do, Korea from 2000 to 2001 to provide the basic data necessary for riverine ecosystem restoration through understanding the growth characteristics of Salix nipponica. Salix nipponica had stomata on only lower side and stomata type was paracytic. Salix nipponica produced many small and light seeds. The seed number per mature ament was 1599.4, seed mass of 0.04 mg, and fertilization rate of 66.1%. Seed germination was little affected by light. Germination rate was high and mean germination time was short Under flooding condition, seeds were germinated normally and were viable after as much as 14 days of flooding. But there were no differences under various water depths on germination rates. Tree age was closely correlated with more stem diameter than tree height and there was no difference of growth rate between male and female tree. Growth rate was most rapid for 2 to 3 years after germination, and length growth was almost stopped for more than 11 years even though mass growth was done. Besides even a second-year indivisual was flowered.

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