• Journal of Veterinary Clinics
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• v.34 no.3
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• pp.222-224
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• 2017

A retrospective study was designed to determine whether there were any differences in prepartal blood biochemical parameters between cows developed subclinical ketosis (SCK) and non-SCK cows. Data on blood biochemistry from 33 Holstein parturient cows (15 SCK and 18 non-SCK cows) in one farm for one year, were compared. Plasma beta-hydroxybutyrate, non-esterified fatty acid and glucose concentrations were utilized in the diagnosis of SCK and showed significant differences between SCK and non-SCK cows after calving. Before calving, however, only plasma total cholesterol concentration in SCK cows was significantly lower than that in non-SCK cows. Consequently, it seems that lower plasma total cholesterol concentration before calving is related to the occurrence of SCK immediately after calving.

• BMB Reports
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• v.56 no.11
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• pp.600-605
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• 2023

Intrahepatic cholangiocarcinoma (ICC) is a bile duct cancer and a rare malignant tumor with a poor prognosis owing to the lack of an early diagnosis and resistance to conventional chemotherapy. A combination of gemcitabine and cisplatin is the typically attempted first-line treatment approach. However, the underlying mechanism of resistance to chemotherapy is poorly understood. We addressed this by studying dynamics in the human ICC SCK cell line. Here, we report that the regulation of glucose and glutamine metabolism was a key factor in overcoming cisplatin resistance in SCK cells. RNA sequencing analysis revealed a high enrichment cell cycle-related gene set score in cisplatin-resistant SCK (SCK-R) cells compared to parental SCK (SCK WT) cells. Cell cycle progression correlates with increased nutrient requirement and cancer proliferation or metastasis. Commonly, cancer cells are dependent upon glucose and glutamine availability for survival and proliferation. Indeed, we observed the increased expression of GLUT (glucose transporter), ASCT2 (glutamine transporter), and cancer progression markers in SCK-R cells. Thus, we inhibited enhanced metabolic reprogramming in SCK-R cells through nutrient starvation. SCK-R cells were sensitized to cisplatin, especially under glucose starvation. Glutaminase-1 (GLS1), which is a mitochondrial enzyme involved in tumorigenesis and progression in cancer cells, was upregulated in SCK-R cells. Targeting GLS1 with the GLS1 inhibitor CB-839 (telaglenastat) effectively reduced the expression of cancer progression markers. Taken together, our study results suggest that a combination of GLUT inhibition, which mimics glucose starvation, and GLS1 inhibition could be a therapeutic strategy to increase the chemosensitivity of ICC.

• Journal of the Korean Institute of Intelligent Systems
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• v.7 no.1
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• pp.34-44
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• 1997

The Belgian Nuclear Research Centre(SCK${\cdot}$CEN) has been a pioneer of the peaceful uses of nuclear energy after over forty years of existence. Recently, SCK${\cdot}$CEN's financial support of doctoral and postdoctoral research in close collaboration with universities has been a vital ingredient for securing a quality profile committed to the pursuit of execllence. FLINS, Fuzzy Logic and Intelligent technologies in Nuclear Science, was initially built within one of the postdoctoral research project at SCK${\cdot}$CEN. Among SCK${\cdot}$CEN's activities which will have an important impact on its scientific future, the application of fuzzy logic and intelligent technologies in nuclear science and engineering opens new domains in radiation protection, safety assessment, human reliability, nuclear reactor control, waste and disposal, etc. In this paper, we review the available literature on fuzzy logic in nuclear applications. We then present the initiative of R&D on fuzzy logic applications at SCK${\cdot}$CEN, namely, (1) safety control for a nuclear reactor, and (2) a safety evaluation model for nuclear transmission lines. By these two examples of nuclear applications, we illustrate the potential use of fuzzy logic in nuclear safety issues.

• Communications of Mathematical Education
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• v.26 no.3
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• pp.301-316
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• 2012

Ball, Thames & Phelps(2008) introduced the idea of Mathematical Knowledge for Teaching(MKT) teacher. Specialized Content Knowledge(SCK) is one of six categories in MKT. SCK is a knowledge base, useful especially for math teachers to analyze errors, evaluate alternative ideas, give mathematical explanations and use mathematical representation. The purpose of this study is to analyze the elementary teacher's SCK. 29 six graders made word problems with respect to division fraction $9/10{\div}2/5$. These word problems were classified four sentence types based on Sinicrope, Mick & Kolb(2002) and then representative four sentence types were given to 10 teachers who have taught six graders. Data analysis was conducted through the teachers' evaluation of the answers(word problems) and revision of students' mathematical errors. This study showed how to know meanings of fraction division for effective teaching. Moreover, it suggested several implications to develop SCK for teaching and learning.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 1993.06a
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• pp.1066-1069
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• 1993

The presence of older installations, in particular nuclear facilities, demands extra studies concerning the safety evaluation of those installations. One of the aspects to deal with is the safety of the several transmission lines in a nuclear installation, for instance the safety of control, safety against fire, etc.. . This paper investigates the use of fuzzy set theory in the inspection of transmission lines of nuclear installations at SCK/CEN, Belgium.

• The Korean Journal of Zoology
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• v.30 no.3
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• pp.311-323
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• 1987

생쥐의 섬 유아세포(MEP)와 종양세포(SCK)를 이용하여 정상세포와 종양세포 사이에 열 감수성의 차이가 있는지의 여부 및 환경의 pH가 이 세포들의 열감수성과 heat shock protein(HSP) 합성에 미치는 영향을 생존곡선과 HSP합성 kinetics등을 써서 검토하였다. MEF와 SCK 세포를 정상 pH(7.4) 또는 산성 pH(6.7)에서 42"C에서 2시간 온열처리 후 3일간에 걸쳐 생존을을 비교해 븐 결과, ME선와 SCK세포 사이에 생득적 열강수성의 차이는 없었고 산성 P광에서는 세포의 종류에 관계없이 열감수성 이 증감되었다. 온열처리의 결과 유도되는 내일성이 conditioning Leat의 크기와 어떤 관계가 있는지를 보기 위해서 45"C에서 5분 또는 20분을 주어본 결과 체은 conditioning heat를 주었을 때 내일성이 신속히 그리고 높은 수준으로 발생하였고, 이러한 열 감수성의 kinetics는 HSP의 합성 kinetics와 잘 일치하였다. 단백질, 특히 HSP 합성에 미치는 PH의 영 향을 알아보기 위해서 46"C에서 6분간의 heat shock를 주어 본 바 전반적인 단백질 및 major HSP의 합성양상에는 별로 차이를 보이지 않았다. 그러나 SCK 세포에 43"C에서 30분의 온열처리를 주고 새로 합성되는 HSPSP의 kinetics를 검토해 본 결과 정상 P반에서는 0-5시간에 합성이 일어나나 산성 PH에서는 3-9시간에 합성이 일어나서 몇시간의 합성지연이 관찰되었다. 아울러 HSP68, HSPTC, HSP87을 Peptidemapping하여 본 결과 HSP68과 HSP70 은 유사한 peptide fragment pattern을 보여 amino acid sequence는 유사하고 기능도 같을 것으로 추론되었으나 HSP87은 전혀 다른 pattern을 보였다. 전혀 다른 pattern을 보였다.

• Nuclear industry
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• v.18 no.7 s.185
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• pp.55-59
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• 1998

• Nuclear Engineering and Technology
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• v.52 no.10
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• pp.2274-2284
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• 2020

The Nuclear Fuel Cycle Code "ANICCA" has been developed by SCK•CEN to answer particular questions about the Belgian nuclear fleet. However, the wide range of capabilities of the code make it also useful for international or regional studies that include advanced technologies and strategies of cycle. This paper shows the main features of the code and the facilities that can be simulated. Additionally, a comparison between several codes and ANICCA has also been made to verify the performance of the code by means of a simulation proposed in the last NEA (OECD) Benchmark Study. Finally, a case study of the Belgian nuclear fuel cycle phase out has been carried out to show the possible impact of the transmutation of the minor actinides on the nuclear waste by the use of an Accelerator Driven System also known as ADS. Results show that ANICCA accomplishes its main purpose of simulating the scenarios giving similar outcomes to other codes. Regarding the case study, results show a reduction of more than 60% of minor actinides in the Belgian nuclear cycle when using an ADS, reducing significantly the radiotoxicity and decay heat of the high-level waste and facilitating its management.

• Radiation Oncology Journal
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• v.17 no.1
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• pp.70-77
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• 1999

Purpose : The expression of p53, P211WAF/CIP, Bcl-2, and Bax underlying the radiation-induced apoptosis in different pH environments using SCK mammary adenocarcinoma cell line was investigated. Materials and Methods Mammary adenocarcinoma cells of hi) mice (SCK cells) in exponential growth phase were irradiated with a linear accelerator at room temperature. The cells were irradiated with 12 Gy and one hour later, the media was replaced with fresh media at a different pHs. After Incubation at 37Microbioiogy, College of Medicine Dong A University for 0$\~$48 h, the extort of apoptosis was determined using agarose gel electrophoresis and flow cytometry. The progression of cells through the cell cycle after irradiation in different pHs was also determined with flow cytometry. Western blot analysis was used to monitor p53, p211WAFfCIP, Bcl-2, and Bu protein levels. Results : The induction of apoptosis by irradiation in pH 6.6 medium was markedly less than that in pH 7.5 medium. The radiation-induced G2IM arrest in pH 6.6 medium lasted markedly longer than that in pH 7.5 medium. Considerable amounts of p53 and p21 proteins already existed at pH 7.5 and increased the level of p53 and p21 significantly after 12 Gy X-irradiation. An incubation at pH 6.6 after 12 Gy X-irradiation did not change the level of p53 and p21 protein levels significantly. Bcl-2 proteins were not significantly affected by radiation and showed no correlation with cell susceptibility to radiation-induced apoptosis in different pHs. An exposure to 12 Gy of X-rays increased the level of Bax protein at pH 7.5 but at pH 6.6, it was slight. Conclusions : The molecular mechanism underlying radiation-induced apoptosis in dinerent pH environments using SCK mammary adenocarcinoma cell line was dependent of the expression p53 and P211YVAF/CIP proteins. We may propose following hypothesis that an acidic stress augments the radiation-induced G2iM arrest, which inhibiting the irradiated cells undergo post-mitotic apoptosis. The effects of environmental acidity on anti-apoptotic and pro-apoptotic function of Bcl-2 family was unclear in SCK mammary adenocarcinoma cell line.

• Korean Journal of Microbiology
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• v.46 no.3
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• pp.270-277
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• 2010

In order to manufacture Bacillus cereus-free fermented soybean products, an antimicrobial agentproducing isolate against B. cereus was obtained from 150 traditionally fermented soybean products. The morphological and biochemical tests and the phylogenetic relationship among 16S rRNA gene sequences indicated that the isolate named as the strain SCK 121057 was most closely related to Bacillus licheniformis. The B. licheniformis isolate began to produce the antimicrobial agent after 48 h of incubation. The agent was nonproteinaceous and insensitive to heat, long term storage and protease K. Electron microscopic observation indicated that the agent attacked the membrane of B. cereus, leaving the ghost cell. The isolate inhibited growth of B. subtilis, Lactobacillus brevis and various types of pathogenic strains including Escherichia coli, E. faecalis, Micrococcus luteus, Staphylococcus aureus, Aspergillus flavus, A. ochraceus, and A. parasiticus as well as B. cereus. After coinoculation of B. licheniformis SCK 121057 and B. cereus in the ratio (as the basis of CFU/g sample) of 10 to 1 on the surface of cooked soybeans, cell numbers of B. cereus had been dramatically reduced after 31 days of incubation compared to those of single inoculation of B. cereus.