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The Establishment of Nitrogen Fixation by Cultured Cell-Rhizobium Association Through Tissue Culture Technique in Soybean (대두조직배양세포(大豆組織培養細胞) - Rhizobium에 의(依)한 질소고정력(窒素固定力))

  • Kang, Sang Jae;Park, Woo Churl
    • Current Research on Agriculture and Life Sciences
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    • v.4
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    • pp.27-35
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    • 1986
  • This experiment was carried out to elucidate the factor of nitrogenase formation and to establish the nitrogen fixation system in mixed culture of cultured cells and rhizobia through tissue culture technique using three soybean varieties, Hwangkeum, Namcheon and D 68-0099 as host plants. The results obtained were as follows; The callus was induced in embryo and radicle, but not in hypocotyl. The most favorable callus induction was caused by the individual application of 2,4-D and NAA at the concentration of 2mg/1 and 4mg/1, respectively, but in case of treating both 2,4-D and kinetin, that was done at the concentration of 0.2mg(2,4-D)/0.05mg(kinetin)per liter. The growth of cultured cell was good at the concentration of 2.0mg(2,4-D)/1 and 0.2mg(2,4-D)/0.05mg(kinetin)per liter. When cultured cells were inoculated with R. japonicum 019 and 011, their growthes were considerably inhibited. The addition of single amino acid inhibited the growth of cultured cells. Hwangkeum was inhibited considerably by methionine and leucine. The inhibition of growth by single amino acid can be abolished by the addition of certain amino acids. The differentiation of adventitious root was good at the concentration of 2.0mg 2,4-D and 0.2mg 2,4-D/0.05mg kinetin per liter. Of three host plants tested with 25 R. japonicum strains, Hwangkeum had affinity for 10 strains, Namcheon for 7 strains and D68-0099 for none. The nitrogen fixing abilities of Hwangkeum and Namcheon caused by cultured cell-Rhizobium association were high in strain 019, 007, and in 007 mixed with 119, respectively.

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Interrelationships and Path Coefficients of Pericarp Characters in Red Pepper(Capsicum annuum L.) (고추 과육중(果肉重) 구성형질(構成形質)의 상관(相關)과 경로계수분석(經路係數分析))

  • Kim, Yang Choon;Park, Gyu Hwan;Choi, Soon Ho
    • Current Research on Agriculture and Life Sciences
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    • v.2
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    • pp.9-14
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    • 1984
  • This study was performed to obtain the effective selection informations for improvement of quality and increase of yield in red pepper. The eight parents and twenty eight crosses from partial diallel were used as materials for estimation of correlations among the pericarp characters, viz, fruit length, fruit width, pericarp thickness (fresh and dry) and pericarp weight (fresh and dry), between pericarp weight and seed weight and between pericarp weight and the percent of seed weight/pericarp weight and path coefficients on fresh and dry pericarp weight. Results were as follows. In $F_1s$, fresh and dry pericarp weight had positive correlations with fruit length, fruit width, and pericarp thickness. Fresh pericarp weight was also positively correlated with dry pericarp weight. Dry pericarp thickness had a negative correlation with fruit length but had positive correlations with fruit width and fresh pericarp thickness. Fresh pericarp thickness had a positive correlation with fruit width. Significantly positive correlations between $F_1s$ and mid-parents were observed in pericarp characters. Pericarp weight had a positive correlation with seed weight but had a negative correlation with the percent of seed weight/pericarp weight. In path coefficient analysis, it was found that fruit length, fruit width and pericarp thickness had direct effects on fresh and dry pericarp weight and that fruit length had the largest direct effect in $F_1s$.

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Changes of Weed Populations Affected by Herbicide Treatment in Apple Fields (제초제(除草劑) 처리(處理)에 의(依)한 과수원(果樹園) 잡초군락(雜草群落)의 변화(変化))

  • Kim, Kil Ung;Shin, Dong Hyun;Cho, Young Woo
    • Current Research on Agriculture and Life Sciences
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    • v.2
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    • pp.34-41
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    • 1984
  • This study was conducted to investigate yearly changes of weeds occurring in orchard and to evaluate an effect of continuous herbicide application on weed population in apple fields. Digitaria sanguinalis, Cephalonoplos segetum, Calystegia hederacea, Polygonum aviculare, Echinochloa crus-galli, and Portulaca oleracea were observed to be the problem weeds in orchard with yearly similar species for a three-year, showing D. sanguinalis was single dominant species for a three-year experimental period. An increase of Simpson's index from 0.22 in the first year to 0.33 in the 3 rd year was observed in the oxyfluorfen treated plot, indicating that most of the weed species were well controlled, while in the untreated plot, D. sanguinalis was relatively single dominant species showing Simpson's index, 0.56 in the 1st year and decreased to 0.32 in the 3 rd year meaning diversification of weed species, and the same trend was also observed in community dominance. Dissimilarity coefficient, 41.6, observed in the weed populations of 1st year and 2nd year, increased up to 58.8 between 1st year and 3 rd year's weed populations in the oxyfluorfen treated plot, indicating that weed community was greatly affected by oxyfluorfen treatment. Based on 3 year's dry weight oxyfluorfen treatment controlled 95 % of weeds such as $149.3g/m^2$ dry weight in the untreated control plot, with a little yearly changes of dry weight. Composition of perennial and biennial weeds was about 59% in the herbicide treated plots and 30% in the untreated control, showing a relative increase of these weeds in the treated plot. However, total dry weight of these weeds was markedly low, showing less than 5% of the untreated one. An annual, D. sanguinalis composed of 70% of total dry weight in the untreated control.

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Estimation of Combining Abilities for Traits of Mice from Diallel Crosses -II. Estimation of Combining Abilities for Baby Weights at Weaning and at the Age of 60 Days (양면교잡(兩面交雜)에 의(依)한 Mouse 주요(主要) 형질(形質)의 결합능력(結合能力) 추정(推定) -II. 이유시(離乳時) 체중(體重)과 60일령(日齡) 체중(體重)에 대한 결합능력(結合能力) 추정(推定))

  • Hyun, Byung Hwa;Choi, Kwang Soo
    • Current Research on Agriculture and Life Sciences
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    • v.4
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    • pp.119-123
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    • 1986
  • The study was conducted to find out the gene effects on body weights at weaning and at the age of 60 days in mice, with 343 progenies from full-dialell crosses of four lines of BALB/c, CBA, C3H and C57BL. The progenies were reared at the Experimental Animal Farm, College of Agriculture, Kyungpook National University from November, 1984 to February, 1985, and data collected from the progenies were analyzed into general combining ability, maternal effects, specific combining ability and reciprocal effects with Harvey's model. General combining ability effects estimated in line-crosses were -0.6033~0.5298 for weaning weights and -0.5086~1.0012 for body weights at the age of 60 days. General combining ability for BALB/c and C57BL were significantly better than general combining ability for CBA and C3H for both traits (P<0.05). Maternal effects for C3H were significantly larger than the maternal effects of BALB/c for both traits (P<0.05). The estimates of maternal effects were -0.9678~0.4609 for weaning weights and -1.1886~0.0729 for body weights at the age of 60 days. Specific combining ability effects were estimated to be significant (P<0.05), and the estimates were -0.1999~0.3380 for weaning weights and -0.4056~0.3317 for body weights at the age of 60 days. Reciprocal effects were found to be largest in BALB/c${\times}$C57BL and BALB/c${\times}$C3H. The estimates were -0.5049 from BALB/c${\times}$C57BL and 0.4972 from BALB/c${\times}$C3H form weaning weights, and -1.0336 from BALB/c${\times}$C57BL and 1.2793 from BALB/c${\times}$C3H for body weights at the age of 60 days.

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Influence of $SiO_2$ Treatment on Guttation in Rice Seedlings Stage (규산(珪酸)이 수도(水稻) 유묘(幼苗)의 일액(溢液)에 미치는 영향(影響))

  • Jeh, Sang Yull;Choi, Jang Soo
    • Current Research on Agriculture and Life Sciences
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    • v.4
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    • pp.1-6
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    • 1986
  • This study was conducted to find out the effect of $SiO_2$ treatment on the $SiO_2$ concentration in guttation liquid, the amount of guttation liquid exudated from the margin of rice leaves, and the relationships between $SiO_2$ concentration in guttation liquid and foliar burn during the rice seedling stage, using two rice varieties;Nagdong-byeo (a Japonica) and Samgang-byeo (a - Indica - Japonica hybrid). Seedlings were cultivated in water solution and enveloped in polyvinyl case ($50cm{\times}150cm{\times}70cm$). $SiO_2$ treatment increased the amount of guttation liquid, the amount of guttation liquid (Nagdong-byeo:4.30mg/plant, Samgang-byeo:4.34mg/plant)) treated with 200ppm of $SiO_2$ was greater than any other level, decreased over 200ppm of $SiO_2$. $SiO_2$ concentration in guttation liquid was increased as application rates of $SiO_2$ increased, showing the positive correlation, and $SiO_2$ in guttation liquid/$SiO_2$ in culture ratio decreased over 100ppm of $SiO_2$. The highest concenitration of $SiO_2$ (Nagdong-byeo:172.6ppm/lcc, Samgang-byeo:199.8ppm/lcc) in guttation liquid was obtained at 12 hrs after $SiO_2$ applied. Foliar burn seemed to be closely related with $SiO_2$ concentration in guttation liquid.

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Soil Analysis on Prediction of Consolidation Settlement in Marine Clays (항만점토(港灣粘土)의 압밀심하량(壓密沈下量) 예측(預測)을 위(爲)한 토질분석(土質分析))

  • Kwon, Moo Nam;Son, Kwang Sik;Lee, Sang Ho
    • Current Research on Agriculture and Life Sciences
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    • v.4
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    • pp.87-94
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    • 1986
  • This study was performed in order to contemplate their correlations between physical and mechanical properties of the marine clays which were collected from main harbors in Korea. The results obtained are as follows: 1. Most of the soils in experimental districts consist of CH. CL. and ML. and they are considered to be still proceeding. 2. The equations of the relationship between compression index and liquid limit are as, follows: CH : $C_c=0.0137$ (LL-22.60) CL : $C_c=0.0123$ (LL-14.64) 3. The relationship between compression index and initial void ratio appears that the higher the plasticity, the easier the slope of the regression line. The equations are as follows : CH : $C_c=0.431$ ($e_o-0.504$) CH : $C_c=0.471$ ($e_o-0.235$) ML : $C_c=0.641$ ($e_o-0.393$) 4. The equations of the relationship between compression index and natural water content are as follows: CH : $C_c=0.0133$ ($W_n-28.27$) CL : $C_c=0.0225$ ($W_n-23.56$) ML : $C_c=0.0106$ ($W_n-16.42$) 5. The relationship between initial void ratio and natural water content, and compression index is highly positive correlation and the equations are as follows : CH : $C_c=0.301$ ($e_o+0.017W_n-1.05$) CL : $C_c=0.141$ ($e_o+0.0567W_n-1.054$) ML : $C_c=0.421$ ($e_o+0.0214W_n-1.121$) 6. The equations of the relationship between initial void ratio and liquid limit, and compression index are as follows : CH : $C_c=0.36$ ($e_o+0.08LL-0.819$) CL : $C_c=0.269$ ($e_o+0.026LL-0.929$) 7. The cohesion of marine clays is no concerned with the increment of depth. The equations of relationship between cohesion and unconfined compression strength are as follows. CH : qu=1.896C+0.0107 CL : qu=1.849C+0.04.

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Effect of Some Essential Oils on Gastric Secretion in Rats and Gastric Motility in Pigeons (몇가지 정유가 Rat의 위액분비 및 비둘기의 위운동에 미치는 영향)

  • Wie, Myung Bok;Park, Joon Hyoung
    • Current Research on Agriculture and Life Sciences
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    • v.5
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    • pp.185-192
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    • 1987
  • Anethole, eugenol, isoeugenol, safrole and isosafrole are ingredients of refined oils which are obtained from some plants and their chemical structures are very similar. They are mainly used as a flavoring agent, food additive, carminative. dental analgesics and for many drugs. But, there is no report about their effect on gastric secretion and gastric motility. To examine the effect of anethole, eugenol, isoeugenol, safrole and isosafrole on gastric secretion in rats and gastric motility in pigeons, this paper was investigated. The results were as follows ; 1. All of 5 essential oils showed significant inhibitory effect compared with control group on gastric secretion at the rate of 1.00 ml/100 g, 0.50 ml/100 g B. W. in the rat. 2. Eugenol, isoeugenol and isosafrole showed significant inhibitory effect on gastric secretion at the rate of 0.25 ml/100 g B. W. in the rat. 3. Isosafrole showed the most inhibitory effect on gastric secretion in 5 essential oils. 4. All of 5 essential oils, in a more or less degree. showed temporary inhibitory effect on gastric motility in the pigeon. 5. In conclusion, all of 5 essential oils showed inhibitory action on gastric secretion in the rat and gastric motility in the pigeon.

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Analysis on Probable Rainfall Intensity in Kyungpook Province (경북지방(慶北地方)의 확률(確率) 강우강도(降雨强度)에 대(對)한 분석(分析))

  • Suh, Seung Duk;Park, Seung Young
    • Current Research on Agriculture and Life Sciences
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    • v.4
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    • pp.77-86
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    • 1986
  • The purpose of this study is to estimate an optimum formula of rainfall intensity on basis of the characteristics for short period of rainfall duration in Kyungpook province for the design of urban sewerage and small basin drain system. Results studied are as follows; 1. The optimum method for Taegu and Pohang, Iwai's and Gumbel-Chow's method are recommended respectively. 2. The opotimum type of rainfall intensity for these area, $I=\frac{a}{\sqrt{t}+b}$ (Japanese type), is confirmed with 2.52~4.17 and 1.86~4.54 as a standard deviation for Taegu and Pohang respectively. The optimum formula of rainfall intensity are as follows. Taegu : T : 200 year - $I=\frac{824}{\sqrt{t}+1.5414}$ T : 100 year - $I=\frac{751}{\sqrt{t}+1.4902}$ T : 50 year - $I=\frac{678}{\sqrt{t}+1.4437}$ T : 30 year - $I=\frac{623}{\sqrt{t}+1.4017}$ T : 20 year - $I=\frac{580}{\sqrt{t}+1.3721}$ T : 10 year - $I=\frac{502}{\sqrt{t}+1.3145}$ T : 5 year - $I=\frac{418}{\sqrt{t}+1.2515}$ Pohang : T : 200 year - $I=\frac{468}{\sqrt{t}+1.1468}$ T : 100 year - $I=\frac{429}{\sqrt{t}+1.1605}$ T : 50 year - $I=\frac{391}{\sqrt{t}+1.1852}$ T : 30 year - $I=\frac{362}{\sqrt{t}+1.2033}$ T : 20 year - $I=\frac{339}{\sqrt{t}+1.2229}$ T : 10 year - $I=\frac{299}{\sqrt{t}+1.2578}$ T : 5 year - $I=\frac{257}{\sqrt{t}+1.3026}$ 3. Significant I.D.F. curves derived should be applied to estimate a suitable rainfall intensity and rainfall duration.

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The Analysis of Forest Vegetation in Mt. Kumjeong (금정산(金井山) 일대(-帶)의 삼림식생분석(森林植生分析))

  • Yun, Chung Weon;Bae, Kwan Ho;Hong, Sung Cheon
    • Current Research on Agriculture and Life Sciences
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    • v.13
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    • pp.31-44
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    • 1995
  • Mt. Kumjeong is located in south-eastern part of the Korean Peninsular($129^{\circ}$01' to $129^{\circ}$05' E, $35^{\circ}$03' to $35^{\circ}$17' N) and the altitude of the summit is 802 meter. In order to find out the vegetation units which made it possible to classify subtropical forest and south-temperate forest, Mt. Kumjeong (which is located in a border of between subtropical forest and south-temperate forest) was selected as a study area. The primary result of actual vegetation analysis in Mt. Kumjeong based upon the vegetation community analysis methods by the ZM-school, and the relationships between vegetation community and two parameters(altitude, topography) analyzed by using coincidence analysis method were summarized as followings. 1. Based upon analytic methods of the vegetation community by the ZM-schools, the primary analytic result of the analysis of vegetation community in Mt. Kumjeong was divided into 10 communities, 9 groups, 2 subgroups. 2. Eurya japonica group of Pinus densiflora community out of 16 vegetation units was thought as a indicator vegetation group which made it possible to border subtropical forest and south-temperate forest. 3. Natural regeneration of Stewartia koreana group was thought to be difficult because seedling and sapling was rarely showed, 4. In relationship between vegetation units and altitude, Carpinus tschonoskii community, Quercus variabilis community, Quercus serrata community, Wisteria floribunda community,. Eurya japonica group of Pinus densiflora community, Pinus thunbergii community mainly distributed below altitude 500 meter, and Quercus mongolica community, Typical group of Pinus densiflora community, Quercus dentata community, Carpinus coreana community, Quercus acutissima community mainly distributed above altitude 500 meter. 5. In relationship between vegetation units and topography, Carpinus tschonoskii community, Quercus variabilis community, Quercus serrata community, Wisteria floribunda community,. Eurya japonica group of Pinus densiflora community distributed below middle slope, Quercus mongolica community, Typical group of Pinus densifiora community, Quercus dentata community, Carpinus coreana community, Pinus thunbergii community Quercus acutissima communily clistributed above midclle slope.

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A research on the introducing the waterproof corrugated cardboard box for the efficient shipment of chinese cabbages and radishes: Focusing on Garak-dong wholesale market as the center

  • Lee, Rae-Hyup;Sun, Il-Suck
    • Asian Journal of Business Environment
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    • v.2 no.1
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    • pp.25-34
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    • 2012
  • It is possible to use pallet for forwarding as chinese cabbages and radishes are general large-scale trading items at the agricultural wholesale market though, however, most of these are forwarded as it have packed in net bags or in P·E bags. Thus, it is still hard for palletizing. The type of packing the product in the net bag makes it difficult for palletizing. It is not a stable shape enough and easily collapsed for pallet loading. Because of this collapsibility, the corrugated cardboard box is being used to enhance forwarding efficiency, but the existing corrugated cardboard box could be crushed easily by moist what is from the agricultural product's property and it also could be squashed by the mass of the loaded box layers on itself. In contrary, the functional waterproof corrugated cardboard box is not collapsed through palletizing and it is efficient for product management with it's ventilation function in respond to pre-cooling effect. Furthermore, because it has various functional shapes as the open type, the partition type and so on, it is effective for maintaining freshness of the product and standardizing the distribution of agricultural product. It is well-known that it is possible to introduce this box to cargo-works of agricultural product. Consequently, the recognition of main distributors about the pallet distribution of the chinese cabbage and the radish was apprehended in this study for activating mechanization of loading and unloading. The survey was conducted to the main distributors such as the forwarder, the auction dealer and the commission merchant with Garak-dong wholesale market as the center. The appropriate packing materials and problems of the existing method for loading and unloading were derived through the survey. Especially, it was focused on analyzing the difference of recognition between the subject groups for the way of using waterproof cardboard corrugated box to deal with the difficult product for packing in normal corrugated box because of the box's absorption of moist from the agricultural product like a chinese cabbage and a radish. Total In the cases of the forwarders and the commission merchants, the net was highly responded as 45%, 74% from each groups for the best packing material for mechanization of distribution and the waterproof corrugated cardboard box was responded as 20%, 22% from each groups as much preferable than multi-stage wooden box. However, for the radish, the waterproof corrugated cardboard box was the best material as 56%, and the auction trader group supported it for 80%. So, the using the waterproof corrugated cardboard box for mechanization of distribution was negative for the chinese cabbage, but it was positive for the radish. The average was 2.42, the standard deviation was 1.24. The negative response(about 55%) was prevailing more than positive response(about 23%). It could be analyzed that even there was the positive recognition for using the waterproof corrugated cardboard box for the radish though the preference for low price of net bag in the chinese cabbage forwarding procedure. Still now, it seems that is a burden for using the waterproof corrugated cardboard box with high price. In the analysis on the recognition differences about using the waterproof corrugated cardboard box for the chinese cabbages and the radish between the forwarders and the commission merchants, generally the negative recognition was prevailing, but the forwarders(2.696) were more positive for using the waterproof corrugated cardboard box than the commission merchants(2.145).

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