• Journal of Life Science
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• v.18 no.10
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• pp.1348-1354
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• 2008

mi transcription factor (MITF) is important in regulating the differentiation of mast cells. In particular, MITF regulates the transcription of the mouse mast cell-specific serine protease (mMCP)-6 gene, which is generally expressed by the connective tissue-type of mast cells. In this study, we investigated alternative isoforms of MITF that regulate transcription of the mMCP-6 gene in bone marrow-derived cultured mast cells in mice. The expression of MITF isoforms was examined by RT-PCR. We observed that MITF-A, -E, -H and -Mc were expressed by mucosal-type mast cells cultured in the presence of IL-3, whereas the connective tissue-type mast cells cultured in the presence of stem cell factor (SCF) expressed MITF-A. Overexpression of MITF isoforms increased luciferase activity through the mMCP-6 promoter in NIH-3T3 cells and elevated the level of mMCP-6 expression in the MC/9 mast cell line. Moreover, mMCP-6 expression in mast cells was significantly inhibited by the depletion of MITF. The transcriptional activity and DNA binding of MITF-A was comparable to that of MITF isoforms, including MITF-E, -H, and -Mc. Our results therefore suggest that MITF-A may be an important isoform of MITF in regulating the transcription of mMCP-6 in mouse connective tissue mast cells.

• Preventive Nutrition and Food Science
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• v.19 no.3
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• pp.136-144
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• 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This study was conducted to evaluate the effects of Platycarya strobilacea S. et Z. (PSE) extract on mouse hair growth and to determine the mechanism of action of PSE. PSE was purchased and its antioxidant activities, such as electron donating ability, total polyphenol content, and flavonoid content were tested. Toxicity during topical treatment was determined by the CCK-8 assay, a cell viability test. Fifteen 4-week-old male C57BL/6 mice were assigned to receive one of three treatments: dimethyl sulfoxide (negative control), minoxidil (positive control) or PSE. Test materials were topically applied to the shaved dorsal skin of each mouse daily for 3 weeks. After 21 days, we observed skin tissue hair follicle morphology and length, mast cell number, and stem cell factor (SCF) expression using hematoxylin and eosin (H&E), toluidine blue, and immunohistochemical staining, respectively. Furthermore, the expression of cytokines involved in hair growth [i.e., insulin-like growth factor (IGF)-1, keratinocyte growth factor (KGF), and transforming growth factor (TGF)-${\beta}1$] was determined by PCR. PSE was found to have very high antioxidant activity. The cell viability rate of PSE-treated mice was markedly higher than that of mice in the control group. We also observed an increase in hair follicle length, strong SCF staining, and a decrease in mast cell number in the PSE group. In addition, PSE-treated mice had higher IGF-1 and KGF expression and lower TGF-${\beta}1$ expression than mice in the minoxidil-treated group. These results suggest that topical application of PSE promotes hair growth by intensifying SCF, suppressing mast cell production, and increasing hair growth-promoting cytokine expression.

• The Korea Journal of Herbology
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• v.28 no.4
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• pp.7-16
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• 2013

Objectives : Brassica campestris var narinosa (BCN), Canavalia gladiata DC semen (CGD) and their combinational prescription (BCN+CGD) have been use to demonstrate to regulate hematopoiesis. In the current study, we investigated whether Brassica campestris var narinosa, Canavalia gladiata DC semen and their combinational prescription is related to hemato-potentiating function using Sca-$1^+$ hematopoietic stem cells (Sca-$1^+HSCs$) as a testing system. Methods : Sca-$1^+HSCs$ isolated from femur in C57bl/6 mice with leukopenia and thrombocytopenia induced by cyclophosphamide (CTX). Then, Real-time PCR was performed to measure the mRNA expression, ELISA and haematopoiesis-related gene (EPO, TPO, IL-3, SCF, c-kit, GM-CSF), the phosphorylation of JAK2, GATA-1 and STAT-5a/b were observed by western blot, and the numbers of $CD117^+/Sca-1^+$ cell and the number of granulocyte erythrocyte monocyte macrophage colony-forming units (CFU-GEMM) and erythroid burst forming units (BFU-E), semisolid clonogenic assay was performed. Result : When Sca-$1^+HSCs$ were treated with Brassica campestris var narinosa, Canavalia gladiata DC semen and their combinational prescription with rIL-3/rSCF, the expression of haematopoiesis-related (EPO, TPO, IL-3, SCF, c-kit, and GM-CSF) were significantly increased at the levels of mRNA as well as production in Sca-$1^+HSCs$. Additionally, CGS enhanced phosphorylation of JAK2, GATA-1, and signal transducer and activator of transcription-5a/b (STAT-5a/b) in Sca-$1^+HSCs$. Furthermore, their combinational prescription (BCN+CGD) significantly enhanced the growth rate of granulocyte erythrocyte monocyte macrophage colony-forming units (CFU-GEMM) and erythroid burst forming units (BFU-E) in vitro. Conclusion : These result suggest that Brassica campestris var narinosa (BCN) and Canavalia gladiata DC have hematopoietic enhancement via hematopoietic cytokine-mediated JAK2/GATA-1/STAT-5a/b pathway, and their combinational prescription (BCN+CGD) has superior hematopoietic enhancement to those of individual extracts.

• Journal of Biomedical Engineering Research
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• v.9 no.2
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• pp.179-184
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• 1988

In this study, we improve the performance of telemetry ECG modem using acoustic coupler. The results are as follows : (1) General active filter is replaced by SCF(switched capaciror filter) improving the LPF and BPF characteristics. (2) ECG Pre-amplifier is installed in ECG modem. (3) The interface circuit is designed to store the ECG waveform in casette recorder.

• The Korean Journal of Ceramics
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• v.4 no.4
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• pp.311-322
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• 1998

American Society for Testing and Materials (ASTM) standard test method PS 070-97 has been created for measuring fracture toughness of advanced ceramics. PS 070-97 includes three test methods which use beams in bending: chevron notch (CNB), single-edged precracked beam (SEPB), and surface crack in flexure (SCF). Supporting data has been collected through several Versailles Advanced Materials and Standards round robins. This paper discusses the evolution of the standard including the rationale for the choice of the three methods and the specifications in the standard. Progress on Standard Reference material 2100 which will have certified values of fracture toughness is presented.

• Proceedings of the Korean Fiber Society Conference
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• 2001.10a
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• pp.83-86
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• 2001

Seed coat fragments (SCF's) are small portions of seed coat that have been broken from the cotton seeds during ginning, a mechanical process that separates the cotton lint from the seed： Seed coat fragments have drawn attention because they are one of the major imperfections that affect the appearance and quality of cotton yarn and fabrics [1,2]. (omitted)

• Electronics and Telecommunications Trends
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• v.31 no.5
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• pp.90-98
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• 2016

컴퓨터 수준의 스마트 디바이스를 이용하는 현재의 이동통신 환경은 처리해야 할 정보량의 급속한 증가에 효율적으로 대응하기 위해서 새로운 시스템 및 네트워크 구조와 기술들을 연구개발하고 있고 여러 가지 후보 기술 중 대규모 고밀도 액세스 네트워크(UDN) 구조 연구에 대한 관심이 높아지고 있다. UDN 환경의 CAPEX 및 OPEX 문제를 해결하기 위해서 최근 액세스 네트워크의 공유 방안에 대한 논의 및 표준화가 진행되어 기술적으로는 인터넷 환경에서 검증된 가상화 기술의 액세스 네트워크 적용을 추진하고 있다. 이러한 동향을 반영하여 최근 SCF 주도로 완성된 소형 셀 기지국 가상화 규격이 현실적이고 실질적인 연구 결과로 평가되어 세부 사항을 분석한다.

• International Journal of Human Ecology
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• v.11 no.2
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• pp.51-61
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• 2010

Using data from the 2007 SCF, this study examined the use of information source for investment decisions of elderly consumers. The results indicated that many elderly consumers (about 88%) involved savings /investment decisions. The elderly used 'Experts' (39.48%) as a major information source for their investment decisions, followed by 'Friends' (24.18%). The results of the multinomial logit analysis suggested that the perceived value, the cost for search, knowledge, risk and some of the demographic factors were significantly related to the choice of the information sources for investments by elderly consumers.

• Proceedings of the Korean Society of Rheology Conference
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• 2006.06a
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• pp.153-156
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• 2006

• Bulletin of the Korean Chemical Society
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• v.10 no.6
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• pp.488-491
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• 1989

Theoretical studies on the proton transfer reaction in a formamide dimer have been done by Ab initio SCF calculation. In this study, we have shown several effects on the potential energy profile of the proton transfer in a formamide dimer, such as the effect of a basis set, the effect of a geometry optimization, and the effect of a distance between proton-donor and proton-acceptor.