• Journal of Environmental Health Sciences
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• v.16 no.1
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• pp.55-65
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• 1990

This study was carried out to investigate the effects on sister chromatid exchanges (SCEs) and chromosome aberrations in PHA or LPS stimulated mouse spleen and bone marrow lymphocytes after an acute whole body irradiation. Frequencies of sister chromatid exchanges were significantly increased with the increased dose(from zero to 400tad) but there was no differences between B-cell and T-cell. By times, the maximum induced SCE levels was observed at 12 hours after irradiation and then returned to base level at one day in 100rad group and three day in 400rad group. There was a significant difference in chromosome aberration with increasing exposure. X-ray irradiated chromosome aberration was long lived relative to SCE. This results show that counting the incidence of SCE may not provide a sensitive system for detecting X-ray exposure.

• Proceedings of the Korea Water Resources Association Conference
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• 2002.05a
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• pp.128-133
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• 2002

• The Journal of Internal Korean Medicine
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• v.32 no.2
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• pp.217-231
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• 2011

Objectives : This study aimed to evaluate the effects of Saengmaekcheongpye-eum (SCE) on a LPS-induced COPD (chronic obstructive pulmonary disease) model. Materials and Methods : The extract of SCE was treated to A549 cells and and LPS-induced COPD mouse model. Then, various parameters such as cell-based cyto-protective activity and histopathological finding were analyzed. Results : SCE showed a protective effect on LPS-induced cytotoxicity in A549 cells. This effect was correlated with analysis for caspase 3 levels, elastin contents, protein levels of cyclin B1, Cdc2, and phospho-Erk1/2, and gene expression of TNF-${\alpha}$ and IL-$1{\beta}$ in A549 cells. SCE treatment also revealed a protective effect on LPS-induced lung injury in COPD mouse model. This effect was evidenced via histopathological findings including immunofluorescence stains against elastin and caspase 3, and protein levels of cyclin B1, Cdc2, and Erk1/2 in lung tissue. Conclusions : These data suggest that SCE has pharmaceutical properties on lung injury. This study thus provides scientific evidence for the efficacy of SCE for clinical application to patients with COPD.

• Korean Journal of Medicinal Crop Science
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• v.25 no.1
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• pp.45-51
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• 2017

Background: This study was performed to evaluate the protective effect of Saururus chinensis ethanol extract (SCE) against styrene toxicity in mouse spermatocyte cells [GC-2spd (ts) cell line]. Methods and Results: Cytotoxicity in mouse spermatocyte cells was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Generation of reactive oxygen species (ROS) was determined using 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA) assay. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and western blotting were performed to quantify the mRNA and protein expression levels, resepectiviely, of stress or apoptosis-related genes including p21, p53, heat shock protein 70 (Hsp70), heat shock protein 90 (Hsp90), Bax, Bcl-2, and caspase-3. The results of the MTT assay showed that $50 {\mu}g/m{\ell}$ SCE did not affect cell viability. ROS generation in mouse spermatocyte cells increased by treatment with $100{\mu}M$ styrene, and decreased by co-treatment with SCE. SCE repressed the mRNA expression of stress-related genes, which increased by styrene treatment. In addition, SCE inhibited the apoptosis of mouse spermatocyte cells by ameliorating mRNA and protein levels of apoptotic genes that were altered by styrene treatment. Conclusions: These results suggest that SCE may alleviate styrene toxicity in mouse spermatocyte cells by reducing ROS stress and regulating genes related to styrene toxicity.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.226-226
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• 2011

최적 저수지운영을 위한 운영률 도출이나 강우-유출 및 수질 모형의 매개변수 추정 문제처럼 비선형적이고 추정해야할 변수의 수가 많은 경우, 수학적으로 모형화하기에 너무 복잡해서 선형계획법, 비선형계획법, 동적계획법 등을 사용하여 최적해를 구할 수 없는 경우도 있다. 이러한 문제에 대해서는 구조적 진화를 통해 최적해를 구하는 방법들이 사용된다. 일반적으로 미지수의 개수가 많아지면 전역최적해를 찾기가 어려워진다. 전역최적해를 찾는 여러 가지 방법들이 수자원 분야에서는 강우-유출모형의 매개변수를 추정하는데 많이 사용되고 있으며, 특히 유전자 알고리즘, SCE-UA 알고리즘 등 전역최적해를 찾는 메타휴리스틱 방법이 많이 사용되고 있다. 전역최적화 방법을 개발하는 연구자들은 최적화방법의 성능을 평가하기 위해 다양한 검사함수(test function)를 만들어 성능을 평가하고 있다. 본 연구에 사용한 검사함수는 Mishra의 연구(2006a, 2006b)에서 사용한 중요하고 복잡한 검사함수이다. 유전자 알고리즘, SCE-UA 알고리즘, DDS 알고리즘을 검사함수 중 전역해를 찾기 어려운 2 차원 함수 2 가지, 다차원 함수 4 가지 함수에 적용하여 각각의 탐색 성능을 평가하였다. 2차원 함수인 Bukin 함수에 대해서는 모든 최적화 방법이 전역최적해를 찾을 수 없었지만, 유전자 알고리즘이 가장 전역최적해에 가까웠고 다음으로 DDS 알고리즘 순서였다. 지역수렴 영역이 많을 것으로 판단되는 10, 30, 50 차원 Michalewicz 함수에 대해서는 DDS 알고리즘으로 구한 최적해가 전역최적해와 매우 근접하였고 다음으로 SCE-UA 알고리즘, 유전자 알고리즘 순이었다. 지역수렴 영역이 상대적으로 다른 함수보다 넓은 10 차원 Schwefel 함수에 대해서는 DDS 알고리즘으로 구한 최적해가 전역최적해와 거의 근접하였고 유전자 알고리즘과 SCE-UA 알고리즘은 매우 큰 편차를 보였다. 40, 80 차원 Schwefel 함수에 대해서는 3 가지 알고리즘 모두 전역최적해와 편차를 보였지만 DDS 알고리즘에 의한 최적해와 다른 두 알고리즘에 의한 최적해는 1 오더(order) 정도의 차이가 났다. 지역수렴 영역이 큰 Michalewicz 함수와 Schwefel 함수에 대한 결과는 매우 흡사한 결과이다. 이상과 같은 결과로, 유전자 알고리즘은 매개변수의 수가 적을 경우 우수한 탐색성능을 가졌으며, SCE-UA 알고리즘은 Griewank, Rastrigin 함수와 같은 형태인 경우 우수한 성능을 보였다. DDS 알고리즘은 전체적으로 우수한 탐색 능력을 가진 것으로 판단된다. 그러므로 수위구간 영역별 저수지운영률 도출을 위한 적절한 최적화방법으로 DDS 알고리즘을 선정하였다.

• Korean journal of food and cookery science
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• v.22 no.1 s.91
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• pp.22-29
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• 2006

Yogurt base was prepared from milk powder $(14\sim18%)$ to which was added 0.4% Saururus chinensis (Lour.) Bail water extract (SCE) and fermented with lactic acid bacteria (a mixed strain of Streptococcus thermophilus and Lactobacillus bulgaricus) at $37^{\circ}C$ for 24 hr. The viscosity of the yogurt added SCE (SCE yogurt) made from 18% milk was higher than that of SCE yogurt containing $14\sim16%$ milk, whereas the pH and titratable acidity of the SCE yogurt were not significantly different on the range of milk contents. The optimal milk concentration for SCE yogurt manufacture was 18%. In order to optimize the preparation of dressing from SCE yogurt, the central composite design was conducted in terms of the yogurt (30, 40, 50, 60, 70 g), the mayonnaise (6, 12, 18, 24, 30 g) and the salt (0.1, 0.3, 0.5, 0.7, 0.9 g) contents. Sensory evaluation was performed and evaluated using a response surface methodology. The optimum ingredient ratio for SCE yogurt dressing was determined to be 61.2% of yogurt, 23.5% of mayonnaise, 0.58% of salt, 0.58% of honey, 1.75% of mustard, 0.23% of Tabasco pepper sauce, 0.94% of wine and 0.04% of white pepper.

• The KIPS Transactions:PartC
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• v.12C no.3 s.99
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• pp.457-464
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• 2005

This paper describes an Adaptive Context-aware Service (ACS) using Model Driven Architecture (MDA)-based Service Creation Environment (SCE) on PARLAY X based service delivery platform in ubiquitous computing environments. It can be expected that both the context-awareness and adaptation in ubiquitous computing environments will be deployed. But the existing context-aware middleware lacks in considering adaptation. Therefore, the object of this paper is to support the architecture and the Application Programming Interface (API) of the network service for both the context-awareness and adaptation in ubiquitous computing environment. ACS is to provide users with the adaptive network service to the changing context constraints as well as detecting the changing context. For instance, ACS can provide users with QoS in network according to the detected context, after detecting the context such as location and speed. The architecture of ACS is comprised of a Service Creation Environment (SCE), Adaptive Context Broker and PARLAY gateway. SCE is to use Context-based Constraint Language (CCL) for an expression of context-awareness and adaptation. Adaptive Context Broker is to make a role of the broker between SCE and PARLAY G/W. PARLAY G/W is to support API for PARLAY X-based service delivery platform.

• Journal of Korea Water Resources Association
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• v.39 no.8 s.169
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• pp.677-690
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• 2006

The LH-OAT (Latin Hypercube One factor At a Time) method for sensitivity analysis and SCE-UA (Shuffled Complex Evolution at University of Arizona) optimization method were applied for the automatic calibration of SWAT model in Bocheong-cheon watershed. The LH-OAT method which combines the advantages of global and local sensitivity analysis effectively identified the sensitivity ranking for the parameters of SWAT model over feasible parameter space. Use of this information allows us to select the calibrated parameters for the automatic calibration process. The performance of the automatic calibration of SWAT model using SCE-UA method depends on the length of calibration period, the number of calibrated parameters, and the selection of statistical error criteria. The performance of SWAT model in terms of RMSE (Root Mean Square Error), NSEF (Nash-Sutcliffe Model Efficiency), RMAE (Relative Mean Absolute Error), and NMSE (Normalized Mean Square Error) becomes better as the calibration period and the number of parameters defined in the automatic calibration process increase. However, NAE (Normalized Average Error) and SDR (Standard Deviation Ratio) were not improved although the calibration period and the number of calibrated parameters are increased. The result suggests that there are complex interactions among the calibration data, the calibrated parameters, and the model error criteria and a need for further study to understand these complex interactions at various representative watersheds.

• Journal of Preventive Medicine and Public Health
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• v.23 no.1 s.29
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• pp.1-10
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• 1990

The protective effect of sodium selenite($Na_2SeO_3$) against the cytogenetic toxicity of heavy metals was investigated on human whole-blood cultures in relation to induction of sister chromatid exchange (SCE) in secondary metaphase chromosome. Methylmercury chloride($CH_3HgCl$), cadmium chloride($CdCl_2$), potassium dichromate($K_2Cr_2O_7$), and sodium selenite caused to the typically dose-dependent increase in sister chromatid exchanges (SCEs) by the concentrations ranging from $0.3{\mu}M\;to\;10{mu}M$. However, the inductions of sister chromatid exchanges by methylmercury chloride or cadmium chloride were inhibited by the simultaneous addition of sodium selenite $1.2{mu}M$. The frequencies of SCE were decreased to the level of control in the molar ratios as 2:1, 1:1, 1:2, and 1:4 of selenium selenite vs. methylmercury chloride, and as 1:1 and 1:2 of selenium selenite vs. cadmium chloride, while the frequencies of SCE induced by potassium dichromate were not changed by the addition of sodium selenite in culture condition. Mitotic indices were decreased in the higher concentrations of chemicals and not significantly changed by the simultaneous addition of sodium selenite to the culture condition containing each chemicals.

• Journal of Preventive Medicine and Public Health
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• v.20 no.1 s.21
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• pp.1-9
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• 1987

Sister chromatid exchanges (SCEs) observed by means of bromodeoxyuridine substitution and fluorescence plus Giemsa (FPG) technique were proposed as a sensitive and quantitative assay for mutagenicity and cytotoxicity in short-term cultures of phytohaemagglutinin (PHA)-stimulated human lymphocytes. Therefore, this study was carried out to investigate the relation between anticancer agents and cytotoxic effects. Chromosomal analysis was performed on metaphase cells that had divided one, two, or three or more times after treatment for SCEs, mitotic indices (MI) and cell cycle kinetics by FPG technique. The results indicate that anticancer agents led to a dose dependent increase in SCE frequency except methotrexate. But, highly inhibited mitotic indices and delayed cell cycle kinetics were observed except for cyclophosphamide. The author suggest that the difference of SCE frequency is due to the differences in the cytotoxic action of anticancer agents, but although the induction of SCEs has a correlation with cell cycle delay, in some cases the induction of SCEs is not always related to cell cycle delay because of different cytotoxic action of anticancer agents.