• 생약학회지
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• 제43권1호
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• pp.59-65
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• 2012

Atopic dermatitis (AD) is a common chronic inflammatory disease of preceding the development of allergic disorders. The incidence of AD is increasing and it causes problems with administrative costs. Therefore, no side effects, easyto- use development of AD treatment is needed. The aim of this study was to evaluate whether PBMCAT, a functional extract from herbal complex was effective to treat the AD mice. AD was induced by patching ovoalbumin on the backside in BALB/ c mouse and then mice were topically treated with PBMCAT. Elidel $cream^{(R)}$ (pimecrolimus, PL) was used as a control. Scratching counts (SC) and clinical skin severity (CSS) were measured, and total serum IgE level was also measured. After inducing AD, SC and CSS were increased. The total serum IgE level was also increased in AD-induced mice. Treatment with PBMCAT significantly decreased SC, CSS, and serum IgE concentration in mice. Especially, treatment of PBMCAT 0.1% in BALB/c mice more effected than PL. These results suggest that the ointment of PBMCAT may enhance the process of AD healing by influencing phase of allergic reacting.

• Environmental Engineering Research
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• 제24권2호
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• pp.324-330
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• 2019

To recycle raw fly ash (RFA), a waste from thermal power plants, it was used to prepare solid catalysts which have many advantages compared with homogenous catalysts. When biodiesel was produced from soybean oil using RFA, only 1.2% of biodiesel conversion was obtained. A metal hydroxide, NaOH, KOH or $Ca(OH)_2$, was mixed with the acid-treated fly ash (ATFA), and the mixture was calcined at $700^{\circ}C$ for 3 h to prepare the solid catalyst. The solid catalyst prepared by mixing ATFA with NaOH, designated as SC-Na, showed a better performance than those prepared by mixing ATFA with KOH or $Ca(OH)_2$, respectively. The optimal mass ratio of ATFA with NaOH was 1:3, at which the proportion of $Na_2O$ increased to 60.2% in SC-Na, and 97.8% of biodiesel conversion was achieved under optimal reaction conditions (2 w% SC-Na relative to oil and 5 mL-methanol/g-oil at $50^{\circ}C$ for 4 h). Finally, a batch operation was repeatedly carried out to test the feasibility of reusing the solid catalyst, and more than 96% biodiesel conversion was stably achieved for the third round of operations. This study shows that RFA was successfully recycled to solid catalysts through a simple preparation method, and the solid catalyst was reused for the production of biodiesel with high conversion.

• 한국초전도ㆍ저온공학회논문지
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• 제11권1호
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• pp.64-68
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• 2009

To keep the superconducting (SC) magnet coils of KSTAR at proper operating conditions, not only the coils but also other cold components, such as thermal shields (TS), magnet structures, SC bus-lines (BL), and current leads (CL) must be maintained at their respective cryogenic temperatures. A helium refrigeration system (HRS) with an exergetic equivalent cooling power of 9kW at 4.5K without liquid nitrogen $(LN_2)$ pre-cooling has been manufactured and installed for such purposes. In this proceeding, we will present the commissioning and initial operation results of the KSTAR HRS. Circuits which can simulate the thermal loads and pressure drops corresponding to the cooling channels of each cold component of KSTAR have been integrated into the helium distribution system of the HRS. Using those circuits, the performance and the capability of the HRS, to fulfill the mission of establishing the appropriate operating condition for the KSTAR SC magnet coils, have been successfully demonstrated.

• KSBB Journal
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• 제16권3호
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• pp.241-245
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• 2001

Mass transfer rates have been measured for the extraction of enzyme from aqueous solution into a reverse-micelle phase at $25^{\circ}C$. The 420 mL vessel was carefully designed to maintain a planar interface between the aqueous and solvent phases, so allowing precise measurement of interfacial area, has been investigated. Sodium di-2-ethylhexyl sulfosuccinate(AOT) was the surfactant used. Factors varied included: agitator speed, pH, ionic strength and surfactant concentration. Samples were taken from the solvent phase at 15min intervals, and the amount of enzyme extracted was measured by UV absorption at 280 nm. The observed Sherwood numbers for the aqueous phase $Sh_1$were correlated interms of the aqueous phase Reynolds number $Re_1$, and modified Schmidt number $Sc_1$. $Sh_1=0.664Re_1^{0.5}Sc_1^{0.33}$

• 한국정보통신학회논문지
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• 제20권9호
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• pp.1755-1762
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• 2016

본 논문에서는 입력전압 범위가 향상된 저면적 이중출력 스위치드 커패시터 DC-DC 변환기를 제안하였다. 기존의 스위치드 커패시터는 면적이 작고 저렴하지만, 효율적인 전압변환을 하는 입력전압의 범위가 좁고 다중출력의 경우 면적이 커지고 전력효율이 낮아진다. 제안된 스위치드 커패시터 DC-DC 변환기는 입력전압에 따라 커패시터 어레이 구조를 변경하여 최적의 효율을 갖는 입력 범위를 증가시켰다. 그리고 두 개의 스위치 어레이를 공유함으로써 스위치와 커패시터 수를 32개에서 25개로 줄였다. 제안된 변환기는 $0.18{\mu}m$ CMOS 공정에서 제작하였다. 시뮬레이션 결과 입력전압 범위는 0.7~1.8V이고, 최대 전력 효율은 90%이며, 칩의 면적은 $0.255mm^2$이다.

• 생명과학회지
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• 제20권12호
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• pp.1801-1806
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• 2010

Klebsiella sp. Sc로부터 birchwood xylan을 분해하는 $\beta$-xylosidase를 분리하였다. 이 $\beta$-xylosidase는 63 kDa의 분자량을 가지는 559개의 아미노산을 암호화하며 1,680개의 뉴클레오타이드로 구성 되는 것으로 밝혀졌다. 기존에 밝혀진 세균성 $\beta$-xylosidase와 상동성을 비교해 보았을 때, Klebsiella oxytoca (KOX)와 90% identities와 95% positives를 나타내었으며 Lactobacillus lactis (LAC, 82%, 90%), Bacillus longum (BLON, 69%, 81%) 그리고 Escherichia coli (ECOLI, 47%, 63%)를 나타내었다. 분리된 $\beta$-xylosidase는 GST-fusion 정제 시스템을 이용하여 순수 정제하였다. 이 효소 활성의 최적 pH는 6.6이었으면 최적 온도는 $55^{\circ}C$였다. TLC를 통해 효소 분해 산물을 관찰한 결과, xylobiose를 분해하여 xylose를 생산하는 것을 관찰 할 수 있었다.

• Journal of Korean Neurosurgical Society
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• 제61권1호
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• pp.10-18
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• 2018

Objective : To investigates the effect of curcumin on proliferation of spinal cord neural stem/progenitor cells (SC-NSPCs) and functional outcome in a rat spinal cord injury (SCI) model. Methods : Sixty adult male Sprague-Dawley rats were randomly and blindly allocated into three groups (sham control group; curcumin treated group after SCI; vehicle treated group after SCI). Functional recovery was evaluated by the Basso, Beattie, and Bresnahan (BBB) scale during 6 weeks after SCI. The expression of SC-NSPC proliferation and astrogliosis were analyzed by nestin/Bromodeoxyuridine (BrdU) and Glial fibrillary acidic protein (GFAP) staining. The injured spinal cord was then examined histologically, including quantification of cavitation. Results : The BBB score of the SCI-curcumin group was better than that of SCI-vehicle group up to 14 days (p<0.05). The coimmunoreactivity of nestin/BrdU in the SCI-curcumin group was much higher than that of the SCI-vehicle group 1 week after surgery (p<0.05). The GFAP immunoreactivity of the SCI-curcumin group was remarkably lower than that of the SCI-vehicle group 4 weeks after surgery (p<0.05). The lesion cavity was significantly reduced in the curcumin group as compared to the control group (p<0.05). Conclusion : These results indicate that curcumin could increase the expression of SC-NSPCs, and reduce the activity of reactive astrogliosis and lesion cavity. Consequently curcumin could improve the functional recovery after SCI via SC-NSPC properties.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제7권1호
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• pp.77-86
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• 2001

전자 상거래의 발전과 더불어 인터넷을 통한 소프트웨어의 분배 및 구매가 활성화되고 있다. 전자 소프트웨어 분배(ESD: Electronic Software Distribution) 모델중 선지불(Buy-first) 방법과 후지불(Try-before-buy) 방법은 소프트웨어의 불법 복제 문제를 해결하지 못한다. 최근 연구된 전자 사용권(EL: Electronic License) 모델은 소프트웨어와 사용권을 분리하여 불법 복제 문제를 해결한다. 그러나 이 방법 역시 소프트웨어에 대하여 다양한 지불 방법을 해결하지 못하다. 따라서 본 논문에서는 새로운 형태의 ESD 모델인 소프트웨어 사용권 관리 에이전트 시스템을 제안한다. 제안된 시스템은 새로운 형식의 사용권(NL: New License)을 제안하여 사용자가 원하는 다양한 지불 방법을 해결한다. 또한 사용 내역서(SC: Software Charge)를 제안하여 사용자가 사용한 소프트웨어 값을 판매자가 받는 것을 보장한다. 제안된 시스템의 에이전트는 NL과 SC를 관리하여 불법 복제 방지 효과 및 시스템간의 확장성을 제공한다.

• 한국수산과학회지
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• 제43권2호
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• pp.117-124
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• 2010

To examine the effective use of seaweeds, sea tangle (Laminaria japonica) was extracted with water and the resultant extracts were fermented with Saccharomyces cerevisiae. Four strains of S. cerevisiae were cultured in aqueous extracts from sea tangle. S. cerevisiae SC-2, which was isolated from a traditional Korean fermented food (Meju), was selected for further study based on the results of a sensory evaluation. No significant differences in proximate compositions, such as moisture, crude protein, crude fat, and crude ash, of the sea tangle extracts before and after fermentation were observed. The reducing sugar decreased as the fermentation period increased, and the contents of some free amino acids were also affected by S. cerevisiae SC-2 fermentation. However, the content of glutamic acid, which is a major taste compound in sea tangle extract, was not affected by fermentation for up to 36 hr by the SC-2 strain. To determine the antioxidant activity of fermented sea tangle extract (fermented for 36 hr by SC-2 strain), the radical scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, and nitric oxide were investigated and xanthin oxidase inhibition assay was performed. The antioxidant activity increased by 8 to 35%. The greatest enhancement of antioxidant activity was seen in the superoxide radical scavenging assay with $100\;{\mu}g/mL$ of raw and fermented sea tangle extract. The anti-inflammatory activity of fermented sea tangle extract was also enhanced. The fermented sea tangle extract showed 34.2% inhibitory activity against nitric oxide synthesis versus 11.9% for raw sea tangle extract at $100\;{\mu}g/mL$ concentration. These results suggest that fermented aqueous extracts from sea tangle are a useful resources.

• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.376-380
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• 1999

Constructs, encoding a single-chain variable fragment of a catalytic antibody 4f4f (scFv-4f4f) with glycosidase activity, were made by combining the coding sequences for the heavy and light chain variable domains with a sequence encoding a linker (GGGGS). Using three different plasmid systems, single-chain antibodies were expressed separately in Escherichia coli, demonstrating significant differences in the expression level and amounts in soluble form of the recombinant protein. The protein expression from pET3a-scFv-4f4f was up to 20% of the total soluble proteins and, more importantly, the proteins were mostly found in a soluble form. An SDS-PAGE analysis of the purified single-chain proteins, yielding higher than 5mg from a 1-1 culture, showed a single band corresponding to its molecular weight of 29,100. A preliminary study shows that the expressed scFv-4f4f is catalytically active. The catalytic parameters for the hydrolysis of p-nitrophenyl-$\beta$-D-glucopyranoside by scFv-4f4f are being investigated.