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http://dx.doi.org/10.5352/JLS.2010.20.12.1801

Isolation, Purification and Characterization of the β-Xylosidase from Klebsiella sp. Sc.  

Lee, Yong-Seok (Department of Biotechnology, College of Natural Resources and Life Science, Dong-a University)
Park, In-Hye (Department of Biotechnology, College of Natural Resources and Life Science, Dong-a University)
Ahn, Soon-Cheol (Department of Microbiology and Immunlogy, Pusan National University school of Medicine)
Choi, Yong-Lark (Department of Biotechnology, College of Natural Resources and Life Science, Dong-a University)
Publication Information
Journal of Life Science / v.20, no.12, 2010 , pp. 1801-1806 More about this Journal
Abstract
A $\beta$-xylosidase encoding gene from Klebsiella sp. Sc was cloned in Escherichia coli. The $\beta$-xylosidase gene consisted of an open reading frame of 1,680 nucleotides and encodes 559 amino acids with a deduced molecular weight of 63 kDa. The deduced amino acid sequence of the $\beta$-xylosidase from Klebsiella sp. Sc exhibits 90% identities and 95% positives compared to those from Klebsiella oxytoca (KOX), Lactobacillus lactis (LAC, 82%, 90%), Bacillus longum (BLON, 69%, 81%) and Escherichia coli (ECOLI, 47%, 63%). The $\beta$-xylosidase was purified by GST-fusion purification system. The pH and temperature optima of the enzyme were 6.6 and $55^{\circ}C$, respectively. The $\beta$-xylosidase hydrolyzes xylobiose to xylose.
Keywords
Klebsiella sp.; $\beta$-xylosidase; glycosyl hydrolase family 43;
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