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Analysis of Somatostatin-Secreting Gastric Delta Cells according to Upper Abdominal Symptoms and Helicobacter pylori Infection in Children

  • Kim, Dong-Uk;Moon, Jin-Hwa;Lee, Young-Ho;Paik, Seung Sam;Kim, Yeseul;Kim, Yong Joo
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.23 no.3
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    • pp.243-250
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    • 2020
  • Purpose: Gastric delta cells (D-cells), which are somatostatin-secreting cells, are the main paracrine inhibitor of acid secretion. The number of D-cells was studied in children presenting with upper gastrointestinal (UGI) disease. Methods: We retrospectively investigated the number of D-cells in the gastric body and antrum through immunofluorescence examinations according to symptoms, endoscopic findings, and Helicobacter pylori infection in 75 children who visited Hanyang University Hospital Pediatrics. Results: The mean patient age was 12.2±3.3 years. The male-to-female ratio was 1:1.4. The mean D-cell number per high-power field in the antrum and body was 20.5 and 12 in children with substernal pain, 18.3 and 10.3 in vomiting, 22.3 and 6 in diarrhea, and 9.3 and 6 in abdominal pain, respectively (p>0.05). According to endoscopic findings, the mean D-cell number in the antrum and body was 14.3 and 6 with gastritis, 14 and 9.3 with reflux esophagitis, 16.7 and 8.7 with duodeno-gastric reflux, 19.3 and 12.7 with gastric ulcer, 16 and 13.7 with duodenitis, and 12.3 and 4 with duodenal ulcer, respectively (p>0.05). The D-cell number in the gastric body was 2.7 and 8.7 in children with current H. pylori infection and non-infected children, respectively (p=0.01), while those in the antrum were 15.5 and 14, respectively, with no statistical significance. Conclusion: The D-cell number was lower in the gastric body of children with current H. pylori infection. Further studies concerning peptide-secreting cells with a control group would provide information about the pathogenic pathways of UGI disorder.

Studies on the Production of Fermented Feeds from Agricultural Waste Products (Part IV) -On the Production of Cellulase by Aspergillus niger and Trichoderma viride- (농산폐기물(農産廢棄物)에서 발효사료(醱酵飼料)의 생산(生産)에 관(關)한 연구(硏究)[제4보(第四報)] -Aspergillus niger와 Trichoderma viride에 의(依)한 Cellulase의 생산성(生産性)에 관(關)하여-)

  • Lee, Ke-Ho;Koh, Jeong-Sam;Lee, Kang-Hup
    • Applied Biological Chemistry
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    • v.19 no.3
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    • pp.139-144
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    • 1976
  • In order to investigate the properties of enzymes from two strains of mold, reported in the previous paper, (1) studies have been made concerning the characteristics of cellulase of Aspergillus niger-SM6 and Trichoderma viride-SM10, and summarized as follows. 1. In the semi-purification the recovery of ${\beta}-glucosidase$ was the highest when 80-90% ethanol was used and 0.8 saturation of $(NH_4)_2SO_4$. 2. The characteristics of the semi-purified enzyme were as follows. Aspergillus niger-SM6 Trichoderma viride-SM10 Optimum pH 3.5 4.0 pH stability 3.0-6.0 3.0-6.0 Optimum temperature $60^{\circ}C$ $60^{\circ}C$ Heat stability below $60^{\circ}C$ below $50^{\circ}C$ Optimum reaction time 30 min. 60 min. Optimum CMC concentration 3% 3% 3. The Km values of CMCase were 0.8% and 1.01 for Aspergillus niger-SM6 and Trichoderma viride-SM10, respectively. 4. In the strain of Aspergillus niger-SM6, there were high activity of xylanase and pectinase.

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Antimicrobial Resistance and Virulence Genes Presence in Escherichia coli Strains Isolated from Gomso Bay, Korea

  • Park, Kwon-Sam
    • Fisheries and Aquatic Sciences
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    • v.16 no.4
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    • pp.221-227
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    • 2013
  • In total, 131 Escherichia coli isolates from surface seawater of the Gomso Bay, of Korea, were analyzed for their susceptibility to 22 different antimicrobials and for genes associated with antimicrobial resistance and virulence. According to the disk diffusion susceptibility test, the resistance to tetracycline was most prevalent (33.6%), followed by that to ampicillin (22.1%), ticarcillin (22.1%), and trimethoprim (16.8%). More than 46.6% of the isolates were resistant to at least one antimicrobial, and 22.9% were resistant to three or more classes of antimicrobials; these were consequently defined as multidrug resistant. We further found that 29 ampicillin-resistant isolates possessed genes encoding TEM-type (93.1%) and SHV-type (6.9%) ${\beta}$-lactamases. Among the 44 tetracycline-resistant isolates, tetA and tetC were found in 35 (79.5%) and 19 (43.2%), respectively, whereas tetB was detected in only three isolates (6.8%). With regard to virulence genes, merely 0.8% (n = 1) and 2.3% (n = 3) of the isolates were positive for the enteroaggregative E. coli-associated plasmid (pCVD432) gene and the enteropathogenic E. coli-specific attaching and effacing (eae) gene, respectively. Overall, these results not only provide novel insight into the necessity for seawater sanitation in Gomso Bay, but they help reduce the risk of contamination of antimicrobial-resistant bacteria.

Advanced Treatment Efficiency of Biological String Media and Oenanthe javanica for Effluent Quality Improvement in the Sedimentation Model Plant (하수의 최종 유출수 수질 개선을 위한 침전조 모형실험에 적용한 끈상 미생물접촉재와 미나리의 고도처리효율에 관한 연구)

  • Bae Joon-Sam;Lee Sang-Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.7 no.3
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    • pp.440-447
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    • 2006
  • This study was aimed at the improvement of the effluent quality of municipal wastewater treatment plant by Oenanthe javanica and Bio-string media. The results were compared with conventional sedimentation tank and pilot sedimentation plant. In this study BOD as organic pollutants, SS, Nitrogen and Phosphorus were tested to evaluate the removal efficiencies for the advanced treatment. The result were as follow: in the Pilot Plant experiment, Tank 2 was fairly high removal efficiency as compared with Tank 1. The removal efficiency of BOD and SS in Tank 2 were as a range of $7.9%{\sim}38.8%,\;14.3%{\sim}60.0%$ and the removal efficiency of T-N, T-P were a range of $9.6%{\sim}31.5%,\;33.0%{\sim}80.0%$ respectively. The removal efficiency of T-P and SS were much higher than BOB and T-N.

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Statistical Verification of Acoustic Emissions Detected during Polymerization Shrinkage of Resin Restoration in Dental Ring (치아/복합레진 수복부의 중합 수축시 검출된 음향방출의 통계적 검증)

  • Gu, Ja-Uk;Choi, Nak-Sam;Arakawa, Kazuo
    • Composites Research
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    • v.23 no.6
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    • pp.39-46
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    • 2010
  • Acoustic emission (AE) signals are detected during the polymerization shrinkage of composite resin restoration in artificial dental ring according to various interfacial treatment conditions. AE amplitudes and the number of AE hit events were compared through the non-parametric statistics of Mann-Whitney method and Kruskal-Wallis method. The AE amplitudes detected from the PMMA and human tooth ring specimens were not significantly different according to adhesive conditions. The stainless steel ring specimen, meanwhile, had a difference in AE amplitude (p<0.05). The quantity of hit events for the human molar dentin specimens of the good bonding state was much less than that for the steel ring specimen but more than that for the PMMA ring specimen. For the same substrate, the better the bonding state, the less the AE hit events (p<0.05). The degree of marginal disintegration measured by SEM was proportional to the amount of AE hit events detected.

Assessment in Habitat Stability of Halophyte by using Mesocosm Experiment (메조코즘 실험에 의한 염생식물의 서식안정성 평가)

  • Ryu, Sung-Hoon;Lee, In-Cheol;Kim, Kyung-Hoi;Yoon, Han-Sam
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.20 no.6
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    • pp.619-626
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    • 2014
  • In this paper, it was constructed the halophyte Mesocosm experimental which was used tidal flat and dredged sediment as a substrate material. Depending on the vegetation and substrate material of Mesocosm, Mesocosm A(tidal flat sediment + Salicornia herbacea), Mesocosm B (only dredged sediment), Mesocosm C(dredged sediment + Salicornia herbacea). Monitoring was carried out of Warter quality factots(Chemical Oxygen Demand(COD), Total Nitrogen(T-N), Total Phosphorus(T-P), water temperature, salinity), Sediment factors(Chemical Oxygen Demand(COD), Total Nitrogen(T-N), Total Phosphorus(T-P)) and growth of Salricornia herbacea. Habitat Stability Index of vegetation was calculating by using the monitoring results. HSI of Mesocosm C was calculated from 0.87 to 0.95 as compared to the relatively high HSI in Mesocosm A, it was evaluated to be able to be used in the restoration and construction of the coastal salt marsh with dredged sediment.

The Effect of Trunk Control Training Using Virtual Reality Game-based Training Program on Balance and Upper Extremity Function of Subacute Stroke Patients (가상현실 게임기반 훈련프로그램을 이용한 체간조절훈련이 아급성기 뇌졸중 환자의 균형 및 상지기능에 미치는 영향)

  • Park, Sam-Ho;Kim, Byeong-Soo;Lee, Myung-Mo
    • Journal of Convergence for Information Technology
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    • v.9 no.5
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    • pp.172-179
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    • 2019
  • The purpose of this study was to investigate the effects of virtual reality game based training on balance and upper limb function in subacute stroke patients. Thirty patients with subacute stroke were randomly assigned to experimental groups(n=15) and control groups(n=15) applying virtual reality game-based training programs. Intervention is applied three times a week for 6 weeks, 30 minutes for 1 time. In the study group, there was a significant improvement in balance ability, upper extremity function, and trunk impairment scale(p<.05), and the difference between the two groups was significant in the BBS, TUG, TIS(p<.05). Based on these results, the virtual reality game based training program is clinically useful exercise program for subacute stroke patients.

Effect of Virtual Reality Based Ring Fit Adventure Core Exercise on the Thickness of the Transverse Abdominis, Internal Oblique and External Oblique Muscle (가상현실 기반의 링 피트 어드벤처 코어 운동이 배가로근, 배속빗근, 배바깥빗근의 두께에 미치는 영향)

  • Yoon, Sam-Won;Yoon, Sung-Young;Park, Han-Kyu
    • Journal of The Korean Society of Integrative Medicine
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    • v.10 no.4
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    • pp.93-102
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    • 2022
  • Purpose : The purpose of this study was to analyze the change in thickness of transvers abdominis, internal oblique, and external oblique when virtual reality based ring fit adventure is applied to young adults in order to investigate the effect of ring fit adventure on core stabilization. Methods : 30 subjects participated in the experiment. Subjects were randomly assigned to two groups. 15 subjects performed ring fit adventure core exercise (experimental group) and 15 subjects bridge and dead bug exercise (control group). The ring fit adventure core exercise program consists of 6 types, 1) bow pull, 2) overhead lunge twist, 3) pendulum bend, 4) seated ring raise, 5) plank, 6) warrior III pose. Each exercise was performed for 5 minutes, for a total of 30 minutes. The bridege and dead bug exercise were performed for 15 minutes each for a total of 30 minutes. All interventions were performed 3 times a week for 4 weeks. Thickness of the abdominal muscles was measured with a ultrasound. The paired t-test was used to compare the thickness of the transverse abdominis, internal oblique, and external oblique before and after intervention, and the comparison between groups was analyzed using the independent t-test. Results : As a result, in the experimental group, thickness of transverse abdominis and internal oblique increased significantly (p<.05), but external oblique decreased significantly (p<.05), and in the control group, thickness of transverse abdominis, internal oblique, and external oblique increased significantly (p<.05). There was a significant difference in external oblique in the difference between groups (p<.05). Conclusion : These study results showed that core exercise using ring fit adventure can reduce external oblique and increased selective muscle activity of transverse abdominis and internal oblique of the deep abdominal muscles, so it is meaningful as an effective intervention for core stabilization.

Studies on Derivatives of 2,2′-Methylene bis (3,4,6-trichloroacetoxy benzene) Part 1. Synthesis and Antimicrobial Sensitivity (2,2-Methylene bis (3,4,6-trichloroacetoxy benzene) 유도체에 관한 연구 제 1보 합성 및 항균성)

  • Yoo, Ju-Hyun;Kim, Jong-Ho;Sa, Hye-Soon;Yoon, Hye-Jung;Yang, Yung;Kim, Yu-Sam
    • Microbiology and Biotechnology Letters
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    • v.1 no.1
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    • pp.51-57
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    • 1973
  • 2,2'-Methylene bis (3,4,6-trichloroacetoxy benzene) had been synthesized from Hexachlorophene. The eleven new derivatives -(2,2'Methylene bis [3,4,6-trichloro o-(${\beta}$-anilinopropionoxy) benzene]: m. p 173∼4$^{\circ}C$, C$\_$31/H$\_$24/N$_2$O$_4$Cl$\_$6/, 2,2' Methylene bis [3,4,6-trichloro (${\beta}$-Cyclohexylaminopropionoxy) benzene]: M. P, 187∼8$^{\circ}C$, C$\_$31/H$\_$36/N$_2$O$_4$Cl$\_$6/, 2,2'-Methylene bis [3,4,6-trichloro (${\beta}$-phenyl hydrazinopropionoxy) benzene]; M. P. 151∼3$^{\circ}C$, C$\_$33/H$\_$28/N$_2$O$_4$Cl$\_$6/, 2,2'-Methylene bis [3,4,6-trichloro (${\beta}$-o-toluidinopropionoxy) benzene]: M. P. 172∼3$^{\circ}C$, C$\_$33/H$\_$28/N$_2$O$_4$Cl$\_$6/, 2,2'-Methylene bis [3,4,6-trichloro (${\beta}$-p-todudinopropionoxy) benzene]: 153∼4$^{\circ}C$, C$\_$33/H$\_$28/N$_2$O$_4$Cl$\_$6/, 2,2'-Methylene bis [3,4,6-trichloro (${\beta}$-o-chloro anilinopropionoxy) benzene]: 170∼2$^{\circ}C$, C$\_$31/H$\_$27/N$_2$O$_4$Cl$\_$8/, 2,2'-Methylene bis [3,4,6-trichloro (${\beta}$-p-sulfamilinopropionoxy) bengene]: M. P. 310-5$^{\circ}C$, C$\_$31/H$\_$24/N$_2$S$_2$O$\_$10/Cl$\_$8/, 2,2'-Methylene bis [3,4,6-trichloro (${\beta}$-piperidinopropionoxy) benzene]: M. P. 168∼2$^{\circ}C$, C$\_$29/H$\_$32/N$_2$O$_4$Cl$\_$6/, 2,2'-Methylene bis (3,4,6-trichloro (${\beta}$-morpholinopropionoxy) benzene]: M. P. 226∼8$^{\circ}C$, C$\_$27/H$\_$28/N$_2$O$\_$6/Cl$\_$6/, 2,2'-Methylene bis (3,4,6-trichloro (${\beta}$-2-amino pyridino propionoxy) benzene]; M. P. 145∼6$^{\circ}C$, C$\_$29/H$\_$22/N$_4$O$_4$Cl$\_$5/-were synthesized by Mannichs reaction as potential antimicrobial agents and their antimicrobial activity were tested against Bacillus subtilis, Pseudomonas ovalis, Escherichia coli, Staphylococcus aureus, aerogenes, Bacillus Acerobacter Polymyxa, Bacillus brevis, Streptomyces griseus, Candida troficalis, Rhodotorula glutinis, Candida utilis, Hansenula anamola, Saccharomyces cerevisiae in vitro. Among them, compounds of benzylamine and p-toludine showed an effective antimibrobial activity againt Bacillus subtilis and Pseudomonas ovalis.

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Studies on the Toxic Effect of Sesame Hulls in the Diet of Albino Rats (참깨피(皮)의 독성효과(毒性效果)에 관(關)한 연구(硏究))

  • Park, Won-Oack;Lee, Yul;Sung, Nak-Eung
    • Korean Journal of Food Science and Technology
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    • v.6 no.3
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    • pp.147-157
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    • 1974
  • This study was undertaken to observe the effect of sesame in the diet of rats (Splague Dowley). Comparisons were made of weight gains, organ weights and the cholesterol, phospholipid and triglyceride content of the blood serum and liver tissue. Rats weighing 67-80 g were used for the study, while rats weighing 160-165 g were used in the study. In the both studies, the experimental animals were fed on sesame mixtures for 24 weeks. The study was conducted to determine the effect of sesame hulls which are known to contain a high proportion of oxalic acid and phytic acid, which are toxic substances causing various physical disorders. The rats were divided into four groups, a control group which was fed on a standard diet and three groups fed with 20%, 10% and 5% mixtures of sesame seed hulls respectively. The study was designed to observe the same effect, but in this case the rats were divided into two groups, one fed with a 5% mixture of whole sesame seeds and the other with a 5% mixture of dehulled seeds. The results are as follows: 1) An examination of weight gains showed that group I and II which were fed on 20% and 10% mixture of sesame hulls were significantly retarded in comparison with the control group(P<0. 05). 2) Comparisons of organ weights, group I (20% mixture) showed relatively lower weights(P<0.05). 3) The cholesterol content of the blood serum and liver tissue of group I (20% mixture) and group II (10% mixture) were significantly higher than that of the control. 4) The group fed on the whole sesame seed diet and that fed on the dehulled seed diet differed significantly from each other. 5) With the results stated above, the investigators could observe that a high content of sesame hulls in the diet caused retardation in growth and might be the cause of many physical disorders. Though these effects are not important in Korea at present, the increasing intake of sesame seeds and oils indicates that it may become an important problems.

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