• Title/Summary/Keyword: S.P.H기법

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Study on Rumen Cellulolytic Bacterial Attachment and Fermentation Dependent on Initial pH by cPCR (cPCR 기법을 이용한 초기배양 pH에 의한 반추위 섬유소 분해 박테리아의 부착 및 발효에 관한 연구)

  • Kim, M.S.;Sung, H.G.;Kim, H.J.;Lee, Sang-S.;Chang, J.S.;Ha, J.K.
    • Journal of Animal Science and Technology
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    • v.47 no.4
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    • pp.615-624
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    • 2005
  • The cPCR technique was used to monitor rumen fermentation and attachment of Fibrobacter succinogenes to cellulose at different pH in the in vitro culture medium. The target fragments of 16S rDNA(445 bp) were amplified from genomic DNA of F. succinogenes with specific primers and internal controls(205 bp) were constructed. Cell counts were estimated from the amounts of genomic DNA, which was calculated from cPCR results. F. succinogenes in pH 6.8 and 6.2 showed apparently higher attachment than in pH 5.8 during all incubation time. There were some difference between pH 6.8 and 6.2 in the degree of attachment, but the different was not significant (P>0.05). Cellulose degradation increased in process of incubation time and the increasing rate was higher when initial pH was higher. The pH in culture medium decreased regardless of initial pH in course of incubation time. After 24 h of incubation, medium pH was dropped by 0.24, 0.58 and 0.16 units from original medium pH 6.8, 6.2 and 5.8, respectively. More gas was produced at higher initial pH in the same manner as in cellulose degradation. In summery, Initial pH of rumen culture in vitro significantly influenced cellulose digestion, gas production, pH change and bacterial attachment. Especially, low pH(5.8) resulted in much lower bacterial attachment and fiber digestion compared to higher medium pH.

Study on Spatial Characteristics of Physicochemical Components of Spring Water in Mts. Geumjeong and Baekyang Area Using Kriging (크리깅 기법을 이용한 금정산-백양산 일대 용천수의 물리화학적 성분의 공간적 분포 특성 연구)

  • 함세영;정재열;류상민;강래수
    • The Journal of Engineering Geology
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    • v.12 no.4
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    • pp.439-457
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    • 2002
  • Sping waters at 60 sites and groundwaters at 6 wells in Mts. Geumjeong and Baekyang area were investigated for measuring nine physicochemical components (temperature, pH, Eh, EC, TDS, DO, salinity, alkalinity and discharge rate). The pH of spring waters ranges from 5.06 to 8.38, alkalinity from 7.93 to 102.21 mg/ㅣ, electrical conductance from 9.5 to $270{\;}\mu\textrm{s}/cm$, Eh from 64.2 to 685.9 mV, DO from 4.57 to 12.13 mg/l, and the discharge rate from 4.26 to 182.2 ml/s. General statistics was carried out to analyze statistical characteristics of those components. To compare the components with one another, regression analyses were carried out. And the components of spring waters were compared to those of groundwaters. Kriging was used to estimate the spatial variation of DO, pH, Eh, EC, alkalinity and discharge rate in the study area. The kriged isopleth maps were made using normalized kriged values to find anomalies of the physicochemical components and to compare anomalies of different components.

Design of Efficient Monitoring and Diagnosis System for Motor Condition Monitoring (모터의 상태 감시를 위한 효율적인 모터 감시 및 진단 시스템의 설계 및 구현)

  • Choi, J.H.;Park, H.S.;Lee, D.Z.;Eo, S.Y.;Kang, T.K.;Jeong, H.P.;Choi, J.P.
    • Proceedings of the KIEE Conference
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    • 2006.10a
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    • pp.167-168
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    • 2006
  • 본 논문은 현장에 운전 중인 모터를 효율적으로 고압 모터 감시 및 진단 기법을 검토하고 현장에 보다 적합한 고압모터 감시 및 진단 시스템을 설계 및 개발에 관하여 기술하였다. 고압 모터의 감시 및 진단 방법은 MCSA(Motor Current Signature Analysis) 기법을 기반으로 하여 순시전력, 공극토크, 진동분석 등 다양한 기법이 있으며 이 기법을 적용한 시스템이 출시되고 있다. 그러나 사용자의 요구 및 편이가 반영되지 않아 그대로 적용하기에는 어려움이 있다. 따라서 본 논문에서는 국내의 실정 및 사용자의 편이 맞도록 진단 시스템을 개발 필요성이 요구되어 다양한 검출 요소를 종합적으로 이용한 보다 현장에 적합하고 유용한 모터 감시 시스템을 설계하였다.

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Molecular Design and Characterization of Biodegradable Crosslinked Copolyesters (생분해성 가교 공중합에스테르의 분자설계 및 특성 연구)

  • Sung, Yong-Kiel;Han, Seung-Jun
    • Polymer(Korea)
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    • v.25 no.1
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    • pp.108-114
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    • 2001
  • Crosslinked poly(glycerol-co-malate)s were synthesized from L-malic acid in Krebs cycle and glycerol. The synthesized polymer was identified by FT-IR spectroscopy. Swelling degrees of the copolymer hydrogels were increased with an increase in pH of the aqueous solution. Hydrolytic behaviors of the crosslinked copolymers were investigated in various pH buffer solutions at 37${\circ}C$. The Hydrolysis of the copolymers proceeded faster with increasing pH of the aqueous solution. Releasing behaviors of the model drug such as diclofenac monosodium salt were also measured in various pH aqueous solutions at 37${\circ}C$. The release concentration of diclofenac monosodium salt from the hydrogel systems was increased with increasing pH. These facts indicate that the unreacted carboxyl and hydroxyl groups in the copolymers are greatly affected by pH in the conditions.

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Anaerobic Acid Tolerance Response in Salmonella typhimurium (Salmonella typhimurium의 혐기적 산내성도 평가)

  • Kim, Young-Chan;Lee, Sun;Lee, Kyung-Mi;Im, Sung-Young;Park, Yong-Geun;Baek, Hyung-Seok;Park, Kyung-Ryang;Lee, In-Soo
    • Journal of Life Science
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    • v.9 no.2
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    • pp.169-175
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    • 1999
  • Salmonella typhimurium can encounter a wide variety of environments during its life cycle. In nature, S. typhimurium can experience and survive dramatic acid stresses that occur in diverse ecological niches ranging from pond water to phagolysosomes. These survival mechanism is aquired by the Acid Tolerance Response(ATR) in Salmonella. The ATR of S. typhimurium is a complex inducible phenomenon in which exposures to slight or moderate low pH will produce a stress response capable of protecting the organism against more severe acid challenges. ATR in Salmonella has two different systems that are called RpoS dependent and independent. We found that ATR in anaerobic was showed RpoS independent because rpoS$\Omega$AP had ATR as S. typhimurium UK1. Using the P22 MudJ(Km, lacZ) operon fusion technique and a lethal selection procedure combining low pH(pH4.5) and sodium acetate(10mM, pH4.5), we isolated LF487 aatA::MudJ which showed acid sensitive in anaerobic condition. aatA locus was determined at 12 min on Salmonella Genetic Map. The survival rate of aatA mutant was showed significantly diminished at pH4.3 than virulent wild type Salmonella in anaerobic condition(5% $CO_2$, 5% H$_2$, 90% $N_2$). Therefore isolated gene was confirmed important gene for anaerobic ATR system.

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A Playing Module under UPnP framework for Various Audio/Video Service (다양한 오디오/비디오 서비스를 위한 UPnP 프레임워크의 재생 모듈 연구)

  • Yun H.;Lee H.;Kim S.;Lee K.;Kim S.;Mah P.;Cho C.;Bae S.
    • Proceedings of the Korean Information Science Society Conference
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    • 2005.11a
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    • pp.247-249
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    • 2005
  • 최근 디지털 TV, DVD 플레이어, 홈시어터와 같은 디지털 A/V 기기들을 통합하여 멀티미디어 컨텐츠를 서비스 받는 홈 네트워크 기술에 대한 관심이 높아지고 있다. 이러한 환경에서 UpnP를 이용한 디지털 기기들의 상태를 모니터링하고, 사용자의 요청과 제어 명령을 전달하여, 멀티미디어 컨텐츠를 서비스 받을 수 있도록 하는 연구는 매우 중요하다. 그러나 UPnP는 TCP/IP 기반의 프로토콜을 사용하기 때문에 사용자 인터랙션에 의한 전송 지연 시간이 발생한다. 그러므로 본 논문에서는 셋탑 박스에 최적화된 UPnP AV 시스템을 구현하고 AV 컨텐츠의 효율적인 재생을 위한 UPnP프레임워크의 재생 기법을 제안한다. UPnP AV 시스템은 미디어 서버가 있고, 미디어 서버에 저장된 비디오/오디오 파일 등을 전송받아 재생하기 위한 셋탑 박스에는 컨트롤 포인트와 UPnP 미디어 렌더러의 통합 모델이 탑재된다. 이러한 기법은 다양한 기기를 제어하고 호환성을 제공하는 UPnP프레임워크가 될 수 있다.

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The Characteristics of Groundwater Quality in the Youngsan and Sumjin River Basins Using Geostatistical Methods (지구통계 기법을 이용한 영산강.섬진강 유역의 지하수 수질특성 연구)

  • 정상용;심병완;김규범;강동환;박희영
    • Journal of the Korean Society of Groundwater Environment
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    • v.7 no.3
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    • pp.125-132
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    • 2000
  • pH, EC and TDS are basic components in the investigation of groundwater quality, and are very important to the preliminary assessment of groundwater quality. These three chemical components investigated at the Youngsan and Sumjin river basins in 1998 suggest that the groundwater quality is generally good in these basins. Linear regression analysis shows that TDS versus EC has an linear correlation, but EC versus pH, and TDS versus pH have nearly no correlation. The relation of TDS and EC is 1.0 mg/1=1.52 $mu\textrm{S}$/cm, and it is the quality of natural water. In geostatistical analysis. three kinds of data are stationary random functions and they have exponential variograms. According to the isopleth maps of the groundwater quality, the groundwater quality of the Youngsan river basin is more contaminated than that of the Sumjin river basin. The isopleth maps of TDS and EC show very similar patterns because of the strong correlation between TDS and EC. The minimum and maximum values of the groundwater quality data are not reflected on the isopleth maps because kriging produces smooth distributions with minimum estimation variances.

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