• Title/Summary/Keyword: S. sobrinus B13

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Effects of the Medicinal Plants Against Streptococcus Sobrinus B13 in Hexane Fraction (수종의 생약 헥산분획 추출물에 의한 Streptococcus sobrinus 억제 효과)

  • Youn, Hye-Jeong;Ha, Myung-Ok;Lee, Kyung-Hee;Park, Young-Nam;Cho, Min-Jeong
    • Journal of dental hygiene science
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    • v.7 no.3
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    • pp.197-200
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    • 2007
  • This study was carried out to assess anticariogenic activity from natural products. The hexane extracts of 11 medicinal plants were tested for the antimicrobial activities against a cariogenic bacterium, S. sobrinus B13. The hexane extracts of 11 medicinal plants such as Alisma orientale, Cyperus rotundus, Saussurea lappa, Atractylodes japonica, Kalopanax pictus, Angelica gigas, Salvia miltorrhiza, Lonicera japonica, Gardenia jasmoides, Aurantii nobilis percarpium and Bupleurum lorgiradiatum were used for this study, and S. sobrinus B13 was cultured with 0.5% extracts of the medicinal plants, in trypticase soy broth medium under 5% $CO_2$ for 0, 6, 12, 24 hours at $37^{\circ}C$. The obtained results were as follows. Immediately after culturing for inoculation, Kalopanax pictus and Cyperus rotundus showed better antimicrobial activities than those of others. After culturing for 6 hours, the order of effective antimicrobial activity was Salvia miltorrhiza, Angelica gigas. After incubation for 12 hours, Salvia miltorrhiza showed the best antimicrobial activity. After incubation for 24 hours Salvia miltorrhiza, Cyperus rotundus, Saussurea lappa, Atractylodes japonica had better antimicrobial activity than others. In conclusion, among Hexane extracts of 11 medicinal plants, Cyperus rotundus, Saussurea lappa, Atractylodes japonica showed effective antimicrobial activity.

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EFFECTS OF POLYPHOSPHATE MIXED IN ACRYLIC RESIN ON THE ATTACHMENT AND GROWTH OF ORAL BACTERIA (Acrylic Resin에 혼합된 Polyphosphate가 미생물의 부착 및 성장에 미치는 영향)

  • Hong, Sun-Hee;Choi, Yeong-Chul
    • Journal of the korean academy of Pediatric Dentistry
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    • v.30 no.1
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    • pp.69-79
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    • 2003
  • The purpose of this present study was to develop a new way of self-curing acrylic resin, using commercially available polyphosphate, Calgon, which is known to be antimicrobial and safe. For the study, polyphosphate(polyP) was blended with acrylic powder and devided into four groups as follows: no polyP(control), 1% polyP, 2% polyP, and 3% polyP. For the experiment, Streptococcus mutans GS5, Streptococcus sobrinus 6715, Streptococcus gordonii G9B and Challis, Porphyromonas gingivalis 2561, and Candida albicans ATCC 90027 were used. Resin specimens in each group were tested in vitro for the purpose of investigating the effect of polyP on the microbial attachment, growth and hydrophobicity of the resin surface. The results were as follows. 1. PolyP added to the acrylic resin decreased attachment of S. mutans GS5, S. sobrinus 6715, S. gordonii G9B. The greater binding inhibition was found in acrylic resin polymerized with polyP at higher concentrations. 2. The addition of polyP to acrylic resin failed to significantly affect the growth of the tested microorganisms. 3. The addition of polyP to acrylic resin seemed to reduce hydrophobicity of the acrylic resin. PolyP in acrylic resin does not seem to exert a direct antibacterial activity, but rather inhibit attachment of oral bacteria, especially mutans streptococci to saliva-coated acrylic resin. The acrylic resin reduces attachment of streptococci may be due to the decreased hydrophobicity caused by polyP added to the resin. PolyP may be included to acrylic resin to inhibit dental caries which often occurs when removable acrylic resin appliance is placed.

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