• Proceedings of the Korean Aquaculture Society Conference
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• 2004.05a
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• pp.156-157
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• 2004

• ALGAE
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• v.30 no.2
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• pp.171-179
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• 2015

Organic light-emitting materials in marine macrophytes from various coastal environments were identified. Twentyeight species from the solvent fractions were examined and identified as candidates for bioorganic light-emitting materials using photoluminescence (PL) spectra and gas chromatography-mass spectrometry. We selected 16 solvent fractions from a total of 1,221 prepared from Ishige okamurae, Sargassum confusum, Grateloupia elliptica, Chondracanthus intermedius, Porphyra yezoensis, Meristotheca papulosa, Gelidium amansii, and Scytosiphon lomentaria. The maximum light-emitting PL spectra appeared at various colors, mainly between blue and green, based on chromaticity coordinates, from solvent fractions of M. papulosa, G. amansii, G. elliptica, P. yezoensis, S. lomentaria, I. okamurae, and C. intermedius. These results will contribute to the development of novel organic light-emitting materials.

• Proceedings of the Korean Aquaculture Society Conference
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• 2006.05a
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• pp.212-213
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• 2006

• The Korean Journal of Ecology
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• v.27 no.3
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• pp.173-177
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• 2004

The number and abundance of epiphytic algae growing on Sargassum thunbergii and their functional forms were examined. Thalli of S. thunbenii were collected on the nine islands of southern coast and at Gyeokpo on the western coast of Korea in summer 2001. A total of 25 species, 6 green (24%), 2 brown (8%), 17 red algae (68%) were identified in the present study. Caulacanthus okamurae, Ulva pertusa, Sphacelaria furcigera were found on the thalli of S. thunbegii that collected at the all regions. Caulacanthus okamurae, Champia bifida and Laurencia venusta grew on the lower parts of S. thunbergii thalli whereas, Polysiphonia spp. attached to the apical parts of the plants. Four functional forms, such as sheet-form (8%), filamentous-form (52%), coarsely branched-form (32%) and articulated-calcareous algal form (8%) were distinguished. The epiphytic algae on the thalli of S. thunbergii are mainly annual opportunistic algae, filamentous-form algae and smaller red algae. Also, most epiphytic seaweeds of S. thunbergii produce and release spores before pseudoperennial host plants are necrotic in late autumn.

• Development and Reproduction
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• v.17 no.3
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• pp.275-287
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• 2013

Although, one of the etiologies of localized lipodystrophy of the subcutaneous connective tissue (cellulite) is the histological alternation of adipose tissue, the characteristics of expression of the components of extracellular matrix (ECM) components during adipogenesis are not uncovered. In this study, the effects of caffeine and Ishige okamurae originated diphlorethohydroxycarmalol (DPHC) on the expression of extracellualr fibers was analyzed with quantitative RT-PCR during differentiation induction of mouse subcutaneous adipose derived stem cells (msADSC) into adipocyte. The expression levels of Col1a, Col3a1, and Col61a were decreased by the adipogenci induction in a time-dependent manners. However, Col2a mRNA and Col4a1 mRNA expressions were oposit to them. Caffeine and DPHC stimulated the changes of the expression of these collagens. Eln mRNA expression was increased by induction. DPHC stimulated the expression of it. Mfap5 mRNA expression was deceased in both adipogenic cell and matured adipocytes. Caffeine suppressed the expression of Mfap5 but the effect of DPHC was different by the concentration. The expression of bioglycan, decorin, and lumican were also modified by caffeine and DPHC in a concentration-dependent manner. Based on this study, we revealed firstly the effects of caffeine and DPHC on the expression of collagens, elastin, and glycoproteins during adipogenesis of msADSCs. Those results suggest that DPHC may have antiadipogenic effect and has more positive effets on normal adipose tissue generation and work as suppressor the abnormality of ECM structure. Such results indicate that DPHC can be applied in keeping the stability of the ECM of adipogenic tissues.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.17 no.1
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• pp.1-8
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• 2012

The objective of the present study was carried out to clarify the effects of deep sea water on the growth and maturation of $Porphyra$ (Rhodophyta, Bangiales). Foliose thalli for indoor culture were collected from Yeongok ($P.$ $okamurae$) in Gangwon Prefecture, Tongyeong ($P.$ $suborbiculata$ f. $latifolia$) and Namhae ($P.$ $yezoensis$ f. $narawaensis$) in Gyongnam Prefecture respectively. Monospores were cultured at five temperatures (5, 10, 15, 20 and $25^{\circ}C$) with a photon irradiance of $80{\mu}m^{-2}s^{-1}$ under photoperiods of 14L:10D and 10L:14D in surface, deep and mixed seawater in respectively. The fast growth of foliose thalli were observed in $P.$ $suborbiculata$ f. $latifolia$ cultured at deep seawater under $15^{\circ}C$ and 10L:14D. In three species, the optimum growth occurred at 10 and $15^{\circ}C$ under deep and mixed seawater and short day-length. In general, monospores from the cultured thalli were liberated within three weeks after incubation under $10-25^{\circ}C$ and both photoperiods. From the result of this study, deep seawater is considered that the natural species of the genus $Porphyra$ can be useful for the development as the new cultivars.

• Natural Product Sciences
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• v.18 no.2
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• pp.130-136
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• 2012

In the present work, we have collected 19 species of macroalgae (9 Phaeophta and 10 Rhodophyta) f rom all around of Korea: Dictyopteris divaricata, D. prolifera, Myelophycus cavus, Papenfussiella kuromo, Petalonia zosterifolia, Petrospongium rugosum, Rugulopteryx okamurae, Sargassum fulvellum, S. muticum, Callophyllis japonica, Gloiopeltis tenax, Gracilaria longissima, Gracilaria vermiculophylla, Grateloupia asiatica, Grateloupia lanceolata, Grateloupia sparsa, Grateloupia turuturu, Grateloupia sp, and Polyopes affinis. The macroalgal species were extracted by 70% ethanol (EtOH) for 24 h and evaluated its inhibitory effects on ${\alpha}$-glucosidase. Among ethanol extracts, Myelophycus cavus showed the most effectively inhibitory activity ($IC_{50}$, 2.17 ${\mu}g/ml$) against ${\alpha}$-glucosidase, followed by Sargassum fulvellum (<$IC_{50}$, 8.13 ${\mu}g/ml$), Dictyopteris prolifera ($IC_{50}$, 16.66 ${\mu}g/ml$), Rugulopteryx okamurae ($IC_{50}$, 50.63 ${\mu}g/ml$), and Petrospongium rugosum ($IC_{50}$, 101.62 ${\mu}g/ml$). Furthermore, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay showed no cytotoxicity on mouse pre-adipocytes cell line (3T3-L1). These results suggest that some edible macroalgae merit further evaluation for clinical usefulness as anti-diabetic functional foods.

• Journal of Aquaculture
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• v.13 no.3
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• pp.253-258
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• 2000

Monthly samplings were made at Yongho-dong, Pusan, on the south eastern coast of Korea from May 1998 to April 1999 to investigate population growth and regeneration pattern of C. okanurae. The growth of erect branches was dependent mainly on the habitat water temperature. Maximum length of an erect branch was 13.4 cm in July and the minimum was 5.1 cm in March; during the corresponding months maximum and minimum weights of the alga were 2.2 and 0.7 g, respectively. During this investigation, gametangia did not occur. Regeneration of excised ramuli was dependent on irradiance. Regeneration rate was the highest under 50 ${\mu}$molm$^{-2}$s$^{-1}$ at 2$0^{\circ}C$. Under the conditions of different combinations of temperature (18, 22, 25, 28 and 1$^{\circ}C$) and irradiance (10, 20, 40, 60 and 100 ${\mu}$molm$^{-2}s$ $^{-1}$) regimes, regeneration of excised erect branch was highly affected by temperature and irradiance. The highest regeneration occurred at 25 $^{\circ}C$ and 20 ${\mu}$molm$^{-2}s$$^{-1}$, whereas the highest growth in length (4.5${\pm}$1.0 cm) and fresh weight (1.2${\pm}$0.7 g) was attained after 15 days of culture.

• ALGAE
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• v.19 no.1
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• pp.69-78
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• 2004

This study was to clarify the variations in a marine ecosystem through examining the species composition of benthic algae in the coast of Dokdo Island during summer (June & August, 2003). The total number of species found in benthic algae was 45 composed of 11% green algae, 29% red algae and 60% brown algae. The dominant species were Enteromorpha linza, Cladophora spp. and Codium frgile for green algae, Ecklonia cave, Eisenia bicyclis, Sargassum confusum, S. patens, S. horneri, Myagropsis myagroides for brown algae and Amphiroa cphedrace. A pusilla, Lithophyllum okamurae, Corallina spp. Laurencia pinnata, L. hamata, Chondria crassicaulit, C. dasyphylla, Gelidium amansii, Neosiphonia yendoi and Polysiphonia yendoi for red algae. The number of benthic algal species decreased more in this study than in previous researches. The whitening of the rocky shore might provoke the decrease of benthic algal biomass and species.

• Journal of Life Science
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• v.21 no.11
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• pp.1501-1510
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• 2011

In recent years novel potential pharmocological candidates have been looked for in animal, seaweed, sponge, fungi and marine bacteria resources. In this study, matrix metalloproteinases (MMPs) that play an important role in metastasis, arthritis, chronic inflammation and wrinkle formation were used as target enzymes to screen therapeutic agents. The inhibitory effects of several marine algae including green algae (5 species), red algae (18 species) and brown algae (4 species) methanolic extracts on MMPs were investigated in human dermal fibroblasts and human fibrosarcoma cell line (HT1080 cells) using gelatin zymography. In human dermal fibroblasts, the inhibition of MMP-2 was observed in Laurencia okamurae, Polysiphonia japonica, Grateloupia lanceolate and Sinkoraena lancifolia of red algae. In contrast, MMP-2 activation was enhanced in Enteromorpha compressa and E. linza of green algae, and Peltaronia bighamiae and Sargassum thunbergii of brown algae. In human fibrosarcoma cells, MMP-9 activation was decreased in the presence of S. thunbergii of brown algae, Polysiphonia japonica in red algae and E. compressa and E. linza of green algae. The interesting finding is that E. compressa and E. linza of green algae, and S. thunbergii of brown algae exhibited a positive effect on MMP-2 in normal cells, but a negative effect on MMP-9 in cancer cell lines. These results suggest that E. compressa and E. linza of green algae, and S. thunbergii of brown algae contain potential therapeutic ingredients for cancer treatment.