• Korean Journal of Food Science and Technology
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• v.27 no.6
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• pp.1017-1027
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• 1995

This study was attempted to investigate physicochemical properties, molecular structural properties of native and acid-treated chestnut starch and chestnut starch gel. The amylose content was 18.9% and X-ray diffraction pattern showed Cb type. Swelling power was increased abruptly in the range of $65^{\circ}C{\sim}75^{\circ}C$ but increased slowly after that and solubility was increased abruptly until $70^{\circ}C$ but increased slowly after that. In amylograms which have different heating temperatures, cooling viscosity at $50^{\circ}C$ was reduced as heating temperature was increased. In molecular structural properties of amylose, ${\lambda}_{max}$ was 640 nm, ${\beta}-amylolysis$ limit was 84.2% and the degree of polymerization was 951 and in those of amylopectin, ${\lambda}_{max}$ was 570 nm, ${\beta}-amylolysis$ limit was 58.2%, the degree of polymerization was 1371 and average chain length was 22.6. In gel chromatography elution profiles of starch and amylose, 4.0% and 11.5% of low molecular weight-molecules($<5{\times}10^5$) were leached out. In gel chromatography elution profiles of soluble starch, the higher heating temperature was, the more high molecular weight-starches were leached out. The elution profiles after debranching amylopectin with pullulanase showed 2.2 of the ratio of peakIII(DP 10-15) to peakII(DP 35-45). Acid hydrolysis extent of 2.2 N HCI-treated starch at $35^{\circ}C$ for 10 days was 96% and hydrolysis rate showed two step pattern which had border line at 4 days. In elution profiles of acid treated chestnut starch, amylopectin peak was disappeared compeletly after 6 hrs and converted short chains of DP 10-15. Amylose content was increased until 6 hrs but decreased after that. Hardness of starch gel made at $75^{\circ}C$ of heating temperature and cohesiveness of starch gel made at $85^{\circ}C$ of heating temperature were the highest. Retrogradation rate of starch gels were relatively high, especially for the starch gel made at $75^{\circ}C$ of heating temperature.

• Tuberculosis and Respiratory Diseases
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• v.41 no.3
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• pp.215-221
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• 1994

Background: It is well known that oxygen free radicals(OFR) play a vital role in the various type of acute lung injury. Among various antioxidant defense mechanisms, the superoxide dismutases(SOD) are thought to be the first line of antioxidant defense by catalyzing the dismutation of two superoxide radicals to yield hydrogen peroxide and oxygen. Eukaryotic cells contain two types of intracellular SOD : cytosolic, dimeric copper/zinc- containing enzyme(CuZnSOD) and mitochondrial, tetrameric manganese-containing enzyme(MnSOD). The purpose of this study is to evaluate the time-dependent gene expression of MnSOD and CuZnSOD in the endotoxin-treated rats, and to compare with the manifestations of LPS-induced acute lung injury in rats. Methods: Total RNA from rat lung was isolated using single step phenol extraction 0, 1, 2, 4, 6, 12, 18, 24 hours after E. coli endotoxin injection(n=3, respectively). RNA was separated by formaldehyde-containing 1.2% agarose gels elctrophoresis, transblotted, baked, prehybridized, and hybridized with $^{32}P$-labeled cDNA probes for rat MnSOD and CuZnSOD, which were kindly donated by Dr. Ho(Duke University, Durham, NC, USA). The probes were labeled by nick translation. Blots were washed and autoradiography were quantitated using laser densitometry. Equivalent amounts of total RNA/gel were assessed by monitoring 28S and 18S rRNA. Results: Endotoxin caused a rise in steady-state MnSOD mRNA levels by 4h with peak mRNA accumulation by 6h. Continued MnSOD mRNA expression was observed at 12h. CuZnSOD mRNA expression was observed from 1h to 24h with peak levels by 18h. Conclusion: These results suggest that SOD palys an important defensive role in the endotoxin-induced acute lung injury in rats.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.24 no.2
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• pp.332-350
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• 2019

Zonal hydrographic section measurements at $39.7^{\circ}N$ were conducted between $129.0^{\circ}E$ and $131.3^{\circ}E$ from August 7 to 25 in 2017 using an underwater glider. The glider traveled about 440 km for about 18 days along the 106 line of the regular shipboard measurements in the National Institute of Fishery Science (NIFS) and obtained twice a hydrographic section with high horizontal resolution. Even under the strong East Korea Warm Current with maximum speed of 0.8 m/s across the section, the glider successfully maintained the designated path within an RMS distance of 400 m. By comparing with the NIFS shipboard hydrographic section, it is confirmed that high spatial resolution measurements obtained from a glider were necessary to properly observe front and eddy variability in the East Sea where a typical spatial scale is smaller than the open oceans. From the glider section measurements, a new lens-shaped eddy was found in the thermocline. The lens-shaped anticyclonic eddy had 10~13 km in horizonal width and about 200 m in height like a typical submesoscale eddy resided within the thermocline, which was firstly named as Korea intrathermocline eddy (Keddy). The Keddy has the distinguishing characteristics of a typical intrathermocline eddy, such as a central core with anomalously weak stratification, a convex shaped lens bounded by the stratification anomaly, an interior maximum of velocity at 170 m, no surface appearance of the geopotential field, a small or comparable horizontal width relative to the first baroclinic Rossby radius of deformation, and the Rossby nubmer of 0.7.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.29 no.1
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• pp.56-76
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• 2024

We for the first time made a successful longest continuous sectional observation in the East Sea by an underwater glider during 95 days from September 18 to December 21 2020 in the Korea along the 106 Line (129.1 °E ~ 131.5 °E at 37.9 °N) of the regular shipboard measurements by the National Institute of Fishery Science (NIFS) and obtained twelve hydrographic sections with high spatiotemporal resolution. The glider was deployed at 129.1 °E in September 18 and conducted 88-days flight from September 19 to December 15 2020, yielding twelve hydrographic sections, and then recovered at 129.2 °E in December 21 after the last 6 days virtual mooring operation. During the total traveled distance of 2550 km, the estimated deviation from the predetermined zonal path had an average RMS distance of 262 m. Based on these high-resolution long-term glider measurements, we conducted a comparative study with the bi-monthly NIFS measurements in terms of spatial and temporal resolutions, and found distinguished features. One is that spatial features of sub-mesoscale such as sub-mesoscale frontal structure and intensified thermocline were detected only in the glider measurements, mainly due to glider's high spatial resolution. The other is the detection of intramonthly variations from the weekly time series of temperature and salinity, which were extracted from glider's continuous sections. Lastly, there were deviations and bias in measurements from both platforms. We argued these deviations in terms of the time scale of variation, the spatial scale of fixed-point observation, and the calibration status of CTD devices of both platforms.

• Journal of Life Science
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• v.34 no.9
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• pp.609-619
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• 2024

Neurodegenerative diseases are marked by the accumulation of toxic misfolded proteins in neurons. Therefore, strategies for the effective prevention and clearance of aggregates are crucial for therapeutic interventions. Cytoplasmic dynein plays a crucial role in the clearance of aggregates by transporting them to the cell center, where lysosomes are enriched and the aggregates undergo extensive autophagic degradation. Previously, we reported evidence for the activation of dynein by N-acetylglucosamine kinase (NAGK) and Reln. In the present study, we explored the effects of NAGK and Reln upregulation on the clearance of aggregates. To upregulate NAGK and Reln genes in HEK293T cells (a human embryonic kidney cell line), CRISPR/dCas9 activation systems (CASs) were used with specific plasmids encoding target-specific 20 nt guide RNA. The effects of this genetic modulation were analyzed in Huntington's disease cellular models, including HEK293T cells and primary mouse cortical cells, where external mutant huntingtin (mHtt, Q74) aggregates were induced. The results showed that the CAS activation of NAGK or Reln, or their combination, significantly reduced the proportion of cells with Q74 aggregates (aggresomes). This effect was reversed by Ciliobrevin D (a dynein inhibitor) and chloroquine (an autophagy inhibitor), indicating the role of dynein-mediated autophagy in aggregate clearance. These findings provide the basis for therapeutic strategies aimed at enhancing neuronal health through targeted gene activation.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.10
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• pp.1391-1399
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• 2009

Two hundred and sixteen 68-week-old Hy-Line brown laying hens were used in a 6-week feeding trial to compare the efficacy of phytases Optiphos (OPT) and Natuphos (NAT), which were isolated from Escherichia coli and Aspergillus niger, respectively. Hens were randomly allotted into six treatments with six replications (six layers in three adjacent cages) per treatment according to their initial BW. The hens were then subjected to one of the following dietary treatments: i) Positive Control (PC; available phosphorus (AP) 0.4%); ii) Negative control (NC; AP 0.2%); iii) NAT1 (NC+250 FTU/kg NAT); iv) NAT2 (NC+500 FTU/kg NAT); v) OPT1 (NC+250 FTU/kg OPT); vi) OPT2 (NC+500 FTU/kg OPT). Feed intake, egg production, egg quality, apparent nutrient digestibility and serum P and Ca concentration were evaluated to compare the effect of the two phytases. Feed intake and eggshell thickness were not affected by the treatments. Superior effects (p<0.05) of OPT were only observed in egg production and egg weight compared with NAT. Characteristics such as eggshell breaking strength, apparent digestibility of N, Ca and P and serum P concentration were equally increased with the supplementation of both phytases (p<0.05), where no significant difference was observed in those characteristics between PC and phytase supplementation at 500 FTU/kg. Equally effective improvements (p<0.05) were also observed in egg production and DM digestibility, where no improvements were observed (p<0.05) between the PC group and the groups with phytase supplementation at 500 FTU/kg. Equal increases in the serum Ca level were observed when the groups with phytase supplementation were compared to the PC group. Overall, the results of this study suggest that NAT and OPT are equally effective at liberating phytate-bound complexes when included in 0.2% available phosphorus diets for 68-week laying hens; either source of phytase can be fed to commercial 68-week laying hens at 500 FTU/kg to correct the negative effects associated with a 0.2% available phosphorus diet. In conclusion, either source of phytase can be fed to commercial first cycle laying hens at 500 FTU/kg to effectively replace inorganic phosphorus when economically justified.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.57-57
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• 2017

Water line은 UV 처리된 호스의 내부에 압력보상 기능이 첨부된 경질의 미로 튜브를 끼워 사용하도록 제작되어 있다. 점적 호스의 외경은 15.8mm이며 대부분 0.5~2.5bar 사이의 압력에 1.5~3.0 L/hr의 유량을 공급한다. 따라서 전적 호스의 내부에 위치한 경질 미로 튜브의 설계는 유량의 압력보상에 밀접한 관계가 있다. 본 연구에서는 경질 미로 튜브를 통과하는 작은 돌기의 수가 압력과 유량에 어떤 영향을 미치는지에 대한 기초적인 실험을 수행하였으며 경질 미로의 압력과 유량 특성에 따른 이론적 해석과의 비교분석을 통하여 새로운 미로의 형상 설계에 활용할 기초적 연구를 수행하고자 하였다. 점적 호스의 성능은 길이(보통 300~400m)에 대한 유량의 균등성을 유지하는 것이며 균등성은 압력보상에 의해 이루어진다. 원통형 미로는 점적 호스의 내부에 삽입되어 원통 부분에 형성된 미로의 길이에 따른 유량의 균등성을 유지하도록 설계되어 있다. 점적 호스의 원통 주변에는 8개의 미로로 구성되어 있으며 미로의 길이를 금속 평면으로 금형 제작하여 투명판에서 미로의 형상을 보이게 하였다. 또한 입구부분에 물을 공급하고 미로의 각 위치별 배출구를 설치하여 압력에 따른 유출량을 전자저울에 측정하여 압력별 유량의 균등성에 대한 데이터를 측정하였다. 유입구의 압력을 0.5, 1.5, 2.5, 3.0bar로 각각 공급하였으며 유출량은 미로의 8개 구간에 따라 측정하였다. 경질 미로의 유출량은 미로의 길이가 짧을수록, 압력이 커질수록 유출량이 높게 나타났다. 또한 미로의 길이가 짧을 경우에는 유출량의 차이가 비교적 크게 나타났으나 미로의 길이가 길수록 압력에 대한 유출량에 대한 차이가 크지 않음을 알 수 있었다. 따라서 경질 미로에서 미로의 길이를 길게 할 경우에는 유출량을 비교적 균일하게 할 수 있음을 보여주고 있다. 반면에 미로의 길이가 길어질수록 압력변화에 따른 유출량이 지수적으로 감소함을 나타내고 있다. 미로의 길이가 7번과 8번에서는 상당히 균일한 성능을 보여주므로 경질 미로의 배출구를 전체적으로 모두 활용함으로써 압력보상을 비교적 균일하게 할 수 있음을 보여주고 있다. 압력보상은 탄성을 가진 실리콘과 미로로 구성될 경우에 가장 큰 효과를 나타내지만 점적 호스와 같이 경질 미로만으로 압력보상을 해야 할 경우에는 경질 미로의 길이가 매우 중요하다.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.56-56
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• 2017

점적 호스 중 PC 드립은 경질 호스의 내부에 Micro-path가 부착된 유로장치와 압력을 보상하는 연질의 실리콘으로 구성되어 있다. 이때 일정압력에서 적정 길이까지 동시에 내부 압력을 유지하며 균등하게 유출구를 통하여 유량을 공급하는 압력보상 기능이 매우 중요하다. 그러나 이 부분에 대한 기술은 선진국에 비해 아직 부족하여 수입에 의존하는 경향이 매우 높다. 뿐만 아니라 선진국 제품에 비해 유로장치 및 연질 실리콘의 탄성이 균일하지 못해 압력에 따른 유량의 편차가 큰 편이다. 본 연구에서는 우리나라 제품의 PC 드립 압력 균등성 기술을 향상시키기 위해 PC 드립의 핵심기술인 연질 실리콘의 성능에 대한 평가와 유량의 편차를 분석하여 문제점을 통한 개선방안을 제시하고자 하였다. 연질 실리콘의 규격은 $17{\times}8{\times}0.85mm$ (가로${\times}$세로${\times}$두께)로서 Water line의 내부에 결합되어 구성된다. PC 드립용 실리콘은 국내 A사 제품을 중심으로 하여 성능을 측정하였다. 측정을 정밀하게 하기 위해 Microtester를 사용하여 일정하게 하중이 부과되도록 하였다. 연질의 실리콘의 경도는 압력 보상에 중요한 기능을 하고 있으며 또한 PC 드립의 경질 미로와 같이 압력을 제어하는 역할을 하고 있다. 국내 A사의 실리콘 경도는 이상적인 실리콘 경도에 비하면 매우 불균일하였다. 세부적으로는 40 이하의 낮은 경도에서는 비교적 일치하였으나 경도 45 이상에서는 큰 차이를 보여주고 있었다. 국내 A사의 실리콘 경도에 따른 공급유량의 특성에서는 실리콘의 경도가 낮을수록 실리콘은 무르며 탄성이 줄어든다. 반면에 경도가 높을수록 탄성이 커지고 딱딱한 특성을 가진다. 동일한 PC 드립에 실리콘의 경도별 특성은 경도에 따른 유량이 반드시 일정하지 않으며 대체로 경도가 높을수록 동일 압력에 대해 유량이 많아지는 것을 알 수 있었다. 압력의 변화에 대해 유량이 일정하게 유지하는 특성을 가질수록 사용범위가 넓어지고 안정성이 높은 것으로 판단되며 실리콘 경도 35~55 범위에서 1~2bar의 공급압력에서 사용하는 것이 적합할 것으로 보여진다. 경도 45의 실리콘을 사용하여 수입 드립, 국내 A사의 드립 및 수입과 국내 혼용시 압력에 따른 공급유량의 특성을 분석하였다. 최대 유량과 최소 유량에서 발생되는 오차는 수입 실리콘이 비교적 작았으나 3.6 bar 이상에서는 유량이 급격히 떨어지는 양상을 보였다. 국내 A사의 제품은 0.8~2.0 bar 까지 성능이 비슷했으나 0.8 bar 이하에서는 오차가 있었으나 높은 압력에서는 오히려 성능이 우수하였다. 수입 드립과 다소 유사한 성능을 나타낸 것이 A사 드립과 수입 실리콘을 혼용하여 사용한 것은 전체적으로 비슷한 양상을 나타내었다. 따라서 실리콘 경도 오차를 고려하면 압력별 유량의 변화는 실리콘의 경도와 밀접한 관계가 있는 것으로 판단되었다.

• Journal of KIISE:Computer Systems and Theory
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• v.26 no.11
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• pp.1373-1381
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• 1999

이 논문에서는 통신망 설계 응용분야의 문제를 그래프 이론 문제로써 고려해 보았다. 개별 기업체가 서로 떨어진 두 곳을 연결하고자 할 때 공용통신망의 회선을 빌려 통신망을 구축하게 되는데 많은 경우 여러 종류의 회선들이 공급됨으로 어떤 회선을 선택하느냐의 문제가 생긴다. 일반적으로 빠른 회선(low delay)은 느린 회선(high delay)에 비해 비싸다. 그러나 서비스의 질(Quality of Service)이라는 요구사항이 종종 종단지연(end-to-end delay)시간에 의해 결정되므로, 무조건 낮은 가격의 회선만을 사용할 수는 없다. 결국 개별 기업체의 통신망을 위한 통로를 공용 통신망 위에 덮어씌워(overlaying) 구축하는 것의 여부는 두 개의 상반된 인자인 가격과 속도의 조절에 달려 있다. 따라서 일반적인 최소경로 찾기의 변형이라 할 수 있는 다음의 문제가 본 논문의 관심사이다. 두 개의 지점을 연결하는데 종단지연시간의 한계를 만족하면서 최소경비를 갖는 경로에 대한 해결을 위하여, 그래프 채색(coloring) 문제와 최단경로문제를 함께 포함하는 그래프 이론의 문제로 정형화시켜 살펴본다. 배낭문제로의 변환을 통해 이 문제는 {{{{NP-complete임을 증명하였고 {{{{O($\mid$E$\mid$D_0 )시간에 최적값을 주는 의사선형 알고리즘과O($\mid$E$\mid$)시간의 근사 알고리즘을 보였다. 특별한 경우에 대한 {{{{O($\mid$V$\mid$ + $\mid$E$\mid$)시간과 {{{{O($\mid$E$\mid$^2 + $\mid$E$\mid$$\mid$V$\mid$log$\mid$V$\mid$)시간 알고리즘을 보였으며 배낭 문제의 해결책과 유사한 그리디 휴리스틱(greedy heuristic) 알고리즘이 그물 구조(mesh) 그래프 상에서 좋은 결과를 보여주고 있음을 실험을 통해 확인해 보았다.Abstract This paper considers a graph-theoretic problem motivated by a telecommunication network optimization. When a private organization wishes to connect two sites by leasing physical lines from a public telecommunications network, it is often the cases that several categories of lines are available, at different costs. Typically a faster (low delay) lines costs more than a slower (high delay) line. However, low cost lines cannot be used exclusively because the Quality of Service (QoS) requirements often impose a bound on the end-to-end delay. Therefore, overlaying a path on the public network involves two diametrically opposing factors: cost and delay. The following variation of the standard shortest path problem is thus of interest: the shortest route between the two sites that meets a given bound on the end-to-end delay. For this problem we formulate a graph-theoretical problem that has both a shortest path component as well as coloring component. Interestingly, the problem could be formulated as a knapsack problem. We have shown that the general problem is NP-complete. The optimal polynomial-time algorithms for some special cases and one heuristic algorithm for the general problem are described.

• Journal of Veterinary Clinics
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• v.28 no.6
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• pp.549-554
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• 2011

FK506 is a widespread immunosuppressive drug after liver transplantion in patients with advanced-stage hepatocellular carcinoma. Dexamethasone is frequently used as co-treatment in cytotoxic cancer therapy, e.g. to prevent nausea, to protect normal tissue or for other reasons. Our aim was to investigate antitumor effects of FK506 in Hep3B cells, one of differentiated human hepatocellular carcinoma cell lines and inhibitory effects of dexamethsone on FK506- induced antitumor effects. Cell injury was evaluated by biochemical assays as cell viability, lactate dehydrogenase (LDH) and reactive oxygen species (ROS) in Hep3B cells. Intracellular calcium concentration ([$Ca^{2+}$]i) and the level of activation of the c-Jun-N-terminal kinase (JNK) and the Bax protein in cultured Hep3B cells was measured. Exposure of 0.1 ${\mu}M$ FK506 to Hep3B cells led to cell death accompanied by a decrease in cell viability and an increase in LDH, ROS and [$Ca^{2+}$]i. FK506 induced an increase in activity of Bax and JNK protein but inhibited the activity of Bcl-2 protein. Treatment of dexamethsone, per se, had no effects on cell viability, LDH and ROS. However, co-treatment of FK506 and dexamethasone diminished the FK506-induced LDH release, ROS generation and JNK activation. These results demonstrate that FK506 has antitumor effect in Hep3B cells but the combination of FK506 and dexamethasone antagonizes the FK506-induced antitumor effects.