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Characterization and Antiviral Effects of Mx Proteins from Various MHC Haplotype Chickens Showing Different Susceptible to Marek's Disease Virus

  • Chang, Kyuug-Soo
    • Biomedical Science Letters
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    • v.16 no.4
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    • pp.229-238
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    • 2010
  • Chicken Mx protein (cMx) induced interferon (IFN) is an antiviral protein to inhibit replication of RNA virus, particularly negative stranded RNA virus, through blockage of transfortation of viral RNA and proteins. In order to determine antiviral effects of cMx from different MHC haplotype chicken, we characterized cMx gene by studying on nucleotide sequencing, antiviral effects to Newcastle disease virus, VSV and MDV, and transcription activities. Three types of eMx genes (2,118 bp) were detected from the different MHC haplotype chickens [B19 (N), B15(F) and B21 (GSP)] chickens, which have showed different susceptible to Marek's disease (MD). Several amino acid substitutions were showed in the cMx. The amino acid 548 and 631 in the cMxs from N and F, chickens susceptible to MD, was Val and Asn which was important on antiviral effects, and showed in resistant cMx. Those in the cMx from GSP, chicken resistant to MD, were same that showed in susceptible cMx. Though every cMx transactivated the expression of the reporter gene, the transcription activation by resistant cMx from N and F was lower compared to that by susceptible cMx from GSP. The decease of the cell growth in the resistant cMx cloned cells was seen in comparison with another cMx clone cells. Replication of NDV and VSV was suppressed in the clones with resistant cMx from N and F. NMx258-transducted cells lack of antiviral effects, and NMx437 or NMx646-transducted cells was showed 60% of antiviral effects compared to NMx705. Mean death time (MDT) and hemaggutination (HA) titer to NDV was long and low in the eggs of N and F lines, but short and high in the egg of GSP line. Interestingly, strong suppression to NDV was observed in the clone with N-Mx and in the eggs of N line. However, the effects of Mx for replication of vvMDV1 have not been. Thus, resistant types of cMx, N- and F-Mx, have showed the anti-viral effects to only RNA virus including NDV and VSV, but not to DNA virus. Antiviral effects of cMx were required whole length of amino acid including Val and Asn in amino acid 548 and 631.

Species Identification and Monitoring of Labeling Compliance for Commercial Pufferfish Products Sold in Korean On-line Markets (국내 온라인 유통 복어 제품의 종판별 및 표시사항 모니터링 연구)

  • Ji Young Lee;Kun Hee Kim;Tae Sun Kang
    • Journal of Food Hygiene and Safety
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    • v.38 no.6
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    • pp.464-475
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    • 2023
  • In this study, based on an analysis of two DNA barcode markers (cytochrome c oxidase subunit I and cytochrome b genes), we performed species identification and monitored labeling compliance for 50 commercial pufferfish products sold in on-line markets in Korea. Using these barcode sequences as a query for species identification and phylogenetic analysis, we screened the GenBank database. A total of seven pufferfish species (Takifugu chinensis, T. pseudommus, T. xanthopterus, T. alboplumbeus, T. porphyreus, T. vermicularis, and Lagocephalus cheesemanii) were identified and we detected 35 products (70%) that were non-compliant with the corresponding label information. Moreover, the labels on 12 commercial products contained only the general common name (i.e., pufferfish), although not the scientific or Korean names for the 21 edible pufferfish species. Furthermore, the proportion of mislabeled highly processed products (n = 9, 81.8%) was higher than that of simply processed products (n = 26, 66.7%). With respect to the country of origin, the percentage of mislabeled Chinese products (n = 8, 80%) was higher than that of Korean products (n = 26, 66.7%). In addition, the market and dialect names of different pufferfish species were labeled only as Jolbok or Milbok, whereas two non-edible pufferfish species (T. vermicularis and T. pseudommus) were used in six commercial pufferfish products described as JolboK and Gumbok on their labels, which could be attributable to the complex classification system used for pufferfish. These monitoring results highlight the necessity to develop genetic methods that can be used to identify the 21 edible pufferfish species, as well as the need for regulatory monitoring of commercial pufferfish products.

Screening of Quinone Reductase Inducers from Agricultural Byproducts Using Mouse Hepatoma Cell Line (Mouse hepatoma 세포를 이용한 농산부산물로부터 quinone reductase활성물질의 탐색)

  • Kim, Jong-Sang;Nam, Young-Jung;Kim, Joo-Won
    • Korean Journal of Food Science and Technology
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    • v.27 no.6
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    • pp.972-977
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    • 1995
  • The induction of phase II enzymes including quinone reductase [NAD(P)H dehydrogenase(quinone): NAD(P)H : (quinone acceptor) oxidoreductase, EC 1.6.99.2] is a major mechanism of whereby a large group of heterogeneous compounds prevent the toxic, mutagenic, and neoplastic effects of carcinogen. Using murine hepatoma cells(Hepalclc7 cells), quinone reductase(QR) inducers as the possible chemopreventive agents were screened from rice bran, wheat bran, soymilk residue, defatted soybean cake, defatted sesame and perilla residues. The 80% methanol extracts of defatted sesame and perilla residues induced quinone reductase significantly while the others did have little effect on the enzyme induction. Thin layer chromatography of the extracts showed that the fastest moving band(Rf=0.70) in the developing solvent of n-butanol : n-propanol : 2N ammonia(10 : 60 : 30) was responsible for the enzyme induction by the 80% methanol extracts of defatted sesame and perilla residues. Further identification of active component(s) is in progress.

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Circumstellar Clumps in the Cassiopeia A Supernova Remnant: Prepared to be Shocked

  • Koo, Bon-Chul;Kim, Hyun-Jeong;Oh, Heeyoung;Raymond, John C.;Yoon, Sung-Chul;Lee, Yong-Hyun;Jaffe, Daniel T.
    • The Bulletin of The Korean Astronomical Society
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    • v.45 no.1
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    • pp.43.1-43.1
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    • 2020
  • Cassiopeia A (Cas A) is a young supernova remnant (SNR) where we observe the interaction of SNR blast wave with circumstellar medium. From the early optical studies, dense, slowly-moving, N-rich "quasi-stationary flocculi" (QSF) have been known. These are probably dense CNO-processed circumstellar knots that have been engulfed by the SNR blast wave. We have carried out near-infrared, high-resolution (R=45,000) spectroscopic observations of ~40 QSF, and here we present the result on a QSF knot (hereafter 'Knot 24') near the SNR boundary of Cas A. The average [Fe II] 1.644 um spectrum of Knot 24 has a remarkable shape with a narrow (~8 km/s) line superposed on the broad (~200 km/s) line emitted from shocked gas. The spatial morphology and the line parameters indicate that Knot 24 has been partially destroyed by a shock wave and that the narrow line is emitted from the unshocked material heated/ionized by the shock radiation. This is the first detection of the emission from the pristine circumstellar material of the Cas A supernova progenitor. We also detected H Br gamma and other [Fe II] lines corresponding to the narrow [Fe II] 1.644 um line. For the main clump where we can clearly identify the shock emission associated with the unshocked material, we analyze the observed line ratios using a shock model that includes radiative precursor. The analysis indicates that the majority of Fe in the unshocked material is in the gas phase, not depleted onto dust grains as in the general interstellar medium. We discuss the non-depletion of Fe in QSF and its implications on the immediate progenitor of the Cas A supernova.

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The Design of the DC traction Protection system and Device for the test line of Light Rail Transit (경전철 시험선로의 직류 보호시스템 및 보호계전기 설계)

  • Jeon, Y.J.;Kim, J.H.;Baek, B.S.;Kim, N.H.;Lee, B.S.;Ahn, J.H.
    • Proceedings of the KIEE Conference
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    • 2002.07b
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    • pp.1229-1231
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    • 2002
  • This paper presents the design of DC protection system of Light Rail Transit system. Especially, the composition and interface for DC Switchgear. Digital protection unit and sort of protection algorithm are focused. DC Switchgear (DCSWGR) for LRT testline consist of 5 different panels with peculiar characteristics are examined. Also Basic actuation principle for DC fault select relay (50F), Line Test Device (LTD), DC Overcurrent(OCR) relay are introduced.

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Wideband Microstrip Slot Array Antenna for Radar Applications

  • Rakluea, P.;Anantrasirichai, N.;Wakabayashi, T.
    • 제어로봇시스템학회:학술대회논문집
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    • 2004.08a
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    • pp.211-214
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    • 2004
  • Microstrip slot array antenna fed by microstrip line is introduced. Slot antenna is designed to operate at 10 GHz for using in radar systems. Antenna have dielectric constant of the substrate is 2.17 (PTFE). In fact, it is study to analyze slot array antenna including feeding line with wide bandwidth. The characteristics of antenna is proposed and analyzed for instance input impedance, $S_{11}$ parameter and far field radiation patterns which these characteristics can also be calculated efficiently and accurately by using FDTD Method.

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A method of extracting edge line from range image using recognition features (거리 영상에서 인식 특정을 이용한 경계선 검출 기법)

  • 이강호
    • Journal of the Korea Society of Computer and Information
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    • v.6 no.2
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    • pp.14-19
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    • 2001
  • This paper presents a new method of 3-D surface feature extraction using a quadratic pol expression. With a range image, we get an edge map through the modified scan line technique this edge map, we label a 3-dimensional object to divide object's region and extract cent corner points from it's region. Then we determine whether the segmented region is a planar or a curved from the quadric surface equation. we calculate the coefficients of the planar su the curved surface to represent regions. In this article. we prove performance of the metho synthetic and real (Odetics) range images.

A Novel Two-party Scheme against Off-line Password Guessing Attacks using New Theorem of Chaotic maps

  • Zhu, Hongfeng
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.11 no.12
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    • pp.6188-6204
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    • 2017
  • Over the years, more password-based authentication key agreement schemes using chaotic maps were susceptible to attack by off-line password guess attack. This work approaches this problem by a new method--new theorem of chaotic maps: $T_{a+b}(X)+T_{a-b}(X)=2T_a(X)T_b(X)$,(a>b). In fact, this method can be used to design two-party, three-party, even in N-party intelligently. For the sake of brevity and readability, only a two-party instance: a novel Two-party Password-Authenticated Key Agreement Protocol is proposed for resisting password guess attack in this work. Compared with the related literatures recently, our proposed scheme can be not only own high efficiency and unique functionality, but is also robust to various attacks and achieves perfect forward secrecy. For capturing improved ratio of security and efficiency intuitively, the paper firstly proposes a new parameter called security/efficiency ratio(S/E Ratio). The higher the value of the S/E Ratio, the better it is. Finally, we give the security proof and the efficiency analysis of our proposed scheme.

n Analysis of Elastohydrodynamic lubrication in Line Contacts-the effect of temperature variation (유체 온도 변화를 고려한 선 접촉면사이의 탄성유체윤활 해석)

  • 서민호;임윤철
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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    • 2001.11a
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    • pp.364-373
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    • 2001
  • This paper describes the variation of lubricant's temperature effects on elastohydrodynamic lubrication. The Newton-Raphson technique was used to solve the simultaneous system of Reynolds and elasticity equations. To show effects of lubricant's temperature, average temperature across the oil film was calculated using the energy equation. Pressure distribution, film shape, and temperature distribution were obtained for fully flooded conjunctions, and various dimensionless speed parameters while load and material parameters were held constant. Minimum film thickness were obtained for various material properties while load and velocity were held constant. It is drawn that the thermal effects have a strong influence on a minimum film thickness under high rolling velocity and slip ratio.

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A Rise in Electric Potential of Telecommunication Line for Electric Power System by Lightning Surges (뇌서지에 의한 전력통신선로의 전위상승)

  • Lee, Bok-Hee;Chang, Sug-Hun
    • Proceedings of the KIEE Conference
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    • 1997.07e
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    • pp.1718-1720
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    • 1997
  • This paper deals with n rise in electric potential of telecommunication line for electric power system by lightning surges. When $1.2/50{\mu}s$ and $8/20{\mu}s$ impulse voltage and current were applied on telecommunication cable, the voltage waveforms at the end terminal and equipment were measured. The telecommunication lines, which consist of 60 conductors and 3.5km in length, have some surge protectors such as preposition lightning arrester and TVSS. In each case, the rise in electric potential was measured for differential mode and common mode, respectively. Also, the rise in ground potential and the induced voltages were measured and analyzed. As a result, a significant rise in ground potential was observed for lightning surge. It can cause failure or malfunction of telecommunication systems.

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