• Korean Journal of Microbiology
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• v.20 no.2
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• pp.89-97
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• 1982

Mutants of plasmid pKM101 modified to enhance mutagenesis were induced and characterized in Salmonella typhimurium. The pKM101 mutant plasmid were transferred normally and stably maintained in cells. They had modified in their ability (i) to enhance the reversion of both point and frameshift mutations, (ii) to protect the cell against UV-irradiation and chemical mutagen treatment, (iii) of ampicillin resistance. A similar modification in enhancement of reversion was also observed in a $uvrB^-$ strains. These results indicated that mutator effect of pKM101 was coded by one plasmid gene.

• Journal of Ginseng Research
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• v.22 no.1
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• pp.11-17
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• 1998

To develop a new ginseng varieties with good quality and high yielding, a lot of individual ginseng plants were selected in the farmer's fields in 1972. Among them, a promising line, 7259-3-1, has been developed through comparative cultivation of several lines selected with pure line separation of local races in Korea Ginseng '||'&'||' Tobacco Research Institute. Preliminary and advanced yield trials were performed for 8 years. It was then designated as "KG 101" and tested in the regional yield and adaptation trials for 10 years (1981-1990). KG101 has a green stem with light violet and orange-yellow fruit and flowers 3-7 days later than local race, Takyungjong. Taproot of KG101 was longer than local race Jakyungjong, and root yield of KG101 was 9% higher than local race Jakyungjong. In red ginseng quality, the rates of Chun-Jeesam (Chun and Jee means 1st and 2nd grade, respectively) were 22.3% and 9.4% for KG101 and Jakyungjong, respectively. In these results, it was clarified that KG101 was superior ginseng line with good quality.y.

• Journal of Ginseng Research
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• v.22 no.4
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• pp.244-251
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• 1998

KG101 was developed by the Korea Ginseng & Tobacco Research Institute. We reported about yield trials and local adaptability test. Growth characteristics and red ginseng quality of KG101 in local performance test can be summarized as follows. Aerial parts growth, stem and leaf, was similar about KG101 and Jakyungjong, 4-year old and 6-year old ginseng. Emergence rate of KG101 was higher than Jakyungjong in the 3 location of local performance test. Taproot diameter of KG101 was smaller than Jakyungjong and taproot shape of KG101 had fitted characteristics, radish type and human body type, to manufactured red ginseng. Raw ginseng grade, 1st and 2nd of KG101 was higher than Jakyungjong's. Chun, Jeesam ratio to manufacture of 2nd grade of raw ginseng of KG101 was 25%, Jakyungjong was 6-13% in 3 location of local performance test. Distribution of red ginseng weight of Jakyungiong was heavier than KG101 in 2 location, Kongju and Jungup, but KG101 was heavier than Jakyungjong in Ichon. Red ginseng quality of KG101 was better than Jakyungiong. Main degrade factor of red ginseng quality was inside cavity and inside white, was not different lines and location.

• Journal of Embryo Transfer
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• v.19 no.2
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• pp.133-145
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• 2004

Serum-contain is commoly used for the production of in vitro-derived bovine embryos. However, were biological activity of serum varies from lot to lot, time consuming to choose better serum with good quality and risks of virus, bacteria and mycoplasma infection. This study established serum-free culture systems of in vitro embryo development to efficiently obtain a large number of blastocysts from ovaries of Hanwoo and oocytes maturation, cell number, tlerance of cryopreservation. Secondly, serum-contain medium is suspected of contributing to the large calf size, dystocia, cersarean sections, calf mortality and confirmed these blastocysts are high quality in terms of cyotolerance, high rates of pregancy and normal birth. For these reasons, Culture media (IVMD101 and IVD101) designed specifically for the preimplantation bovine embryo are rather simplistic, being based on salt solutions with additional energy substrates and growth factors. An improved serum-free medium (IVMD101) was developed for bovine oocytes maturation in vitro. Proportions of embryos developing to the blastocyst stage cultured in both IVD101(32.4%) and IVD101(34.5%) serum-free media were higher than in TCM199+10% FBS(12.4%) serumcontaining medium. Futhermore, the cell numbers per blastosyst obtained in the serum-free media were superior to those of blastocysts developed in serum-supplemented medium. Also, cell numbers of blastocysts obtained in the serum-free media were similar with blastocysts derived in vivo. Survival rate blastocysts after 24 hr incubation after thawing, the blastocysts cultured in both IVD101(94.5%) and IVD101(95.8%) serum-free media were higher than in TCM199+10% FBS (52.5%) serum-containing medium. After 72 hr incubation after thawing, hatching rates of blastocysts developed in IVD101(78.4%) and IVMD101(83.7%) were sighnificantly higher than that developed in the serum-supplemented medium(32.0%). The pregnancy rates almost not different between fresh blastocysts(38.2%) and frozen blastocysts(34.9%). The results suggested that the improved serum-free media(IVMD101 and IVD101) offer several advantages over culture in serum-cotaining medium, including increased rates of blastocyst formation and high cel numbers. Additionally, the survival and hatching rates of embryos product in serum-free media after post-thaw culture were superior to those of embryos produced in the serum-containg medium and useful for the production of high quality bovine embryos for cryo-preservation. These improved serum-free media are beneficial not only for the study of the mechanisms of early embryogenesis but also for mass production of good quality embryos for embryo transfer, cloning and transgenesis.

• Proceedings of the Korean Magnestics Society Conference
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• 2003.12a
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• pp.101-101
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• 2003

• Journal of Periodontal and Implant Science
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• v.16 no.1
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• pp.101-101
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• 1986

• The Plant Pathology Journal
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• v.24 no.4
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• pp.469-473
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• 2008

We have previously obtained a representative isolate KU101 of the predominant Penicillium species from rice under indoor storage conditions. In this study we attempted to characterize isolate KU101 using its morphological and molecular characteristics. When the micro- and macroscopic characteristics of isolate KU101 were compared with the P. islandicum reference isolate KCCM 34763, isolate KU101 was generally identical to those of isolate KCCM 34763, however, isolate KU101 grew faster and produced more orange to red pigments than isolate KCCM 34763. In a molecular-based identification, the nuclear sequence of the ITS1-5.8S-ITS2 region of isolate KU101 was most closely related to that of P. islandicum. Therefore, these results indicated that isolate KU101 from stored rice could be identified as P. islandicum, some isolates of which are known to produce mycotoxins.

• Archives of Pharmacal Research
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• v.18 no.6
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• pp.423-426
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• 1995

SM-101 is a mixture of metampicillin and sulbactam(2:1). The antibacterial activities of SM-101 were compared with those of metampicillin, piperacillin and Augmentin. It showed powerful antibacterial activities against major strains. Except P. anruginosa and S. marcescens, the in vitro antibacterial activity of SM-101 was higher than those of metampicillin, piperacillin and Augmentin against Staphylococcus spp., Streptococcus spp., Moganella morganii, E. Coil, and Proteus spp. The $ED_{50}$ values of SM-101 were two-fold or greater than those of metampicillin, piperacillin and Augmentin against $\beta-lactamase$ producing strains, p. mirabilis GN79 and M. morganiii MB4-11. The in vivo efficacy of SM-101 was more active than metampicillin and pipeeracillin and similar to Augmentin against S. aureus Smith, E coli MB4-01 and K. pneumoniae MB4-02.

• Journal of the Korean Society of Tobacco Science
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• v.10 no.1
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• pp.27-36
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• 1988

This experiment was tarried out to investigate the effect of plant spacing and amount of side dressing on the yield, quality and protein pattern in burley 21 and KB 101. The results obtained were summarized as follow. 1 Yield and alkaloid content are increased In high plant population and side dressing. 2 . Quality is not affected by plant population and side dressing. 3 . Nitrogen content is decreased by late growing stage. 4. The bands of burley 21 seed are fewer than KB 101 seed bands in protein pattern. 5. In protein pattern, the bands of KB 101s leave are thicker than that of burley21s leave between 18,000∼14,000 of molecular weight.

• Journal of Korea Entertainment Industry Association
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• v.13 no.5
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• pp.37-46
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• 2019

The PRODUCE 101 (Chinese version《创造101》) program is an "Idol Nurture" reality show bought by China's Tencent Video from the copyright of the Korean PRODUCE 101 program. The program's "Idol Nurture" pattern is concentrated in the four aspects of the "Semi-finished" training objects, the complementary nurturing goals, the key points of storytelling, and the extreme empowerment of training subjects. The "Idol Nurture" process is fully presented through the programming of the training processes inside entertainment companies, and the foregrounding of the back stages of the recording, trainees and entertainment companies. The "Idol Nurture" pattern of the program has attracted great attention and has been applauded by many because it better satisfies audiences' diversion utility, personal relation utility, and personal identity utility. This pattern of "Idol Nurture" program can provide insightful reference and valuable experience to the development of other reality talent shows.