• Asian-Australasian Journal of Animal Sciences
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• v.33 no.12
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• pp.2021-2030
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• 2020

Objective: Quantitative polymerase chain reaction (qPCR) has been extensively used in the field of mesenchymal stem cell (MSC) research to elucidate their characteristics and clinical potential by normalization of target genes against reference genes (RGs), which are believed to be stably expressed irrespective of various experimental conditions. However, the expression of RGs is also variable depending on the experimental conditions, which may lead to false or contradictory conclusions upon normalization. Due to the current lack of information for a clear list of stable RGs in bovine MSCs, we conducted this study to identify suitable RGs in bovine MSCs. Methods: The cycle threshold values of ten traditionally used RGs (18S ribosomal RNA [18S], beta-2-microglobulin [B2M], H2A histone family, member Z [H2A], peptidylprolyl isomerase A [PPIA], ribosomal protein 4 [RPL4], succinate dehydrogenase complex, subunit A [SDHA], beta actin [ACTB], glyceraldehyde-3-phosphate dehydrogenase [GAPDH], TATA box binding protein [TBP], and hypoxanthine phosphoribosyltrasnfrase1 [HPRT1]) in bovine bone marrow-derived MSCs (bBMMSCs) were validated for their stabilities using three types of RG evaluation algorithms (geNorm, Normfinder, and Bestkeeper). The effect of validated RGs was then verified by normalization of lineage-specific genes (fatty acid binding protein 4 [FABP4] and osteonectin [ON]) expressions during differentiations of bBMMSCs or POU class 5 homeobox 1 (OCT4) expression between bBMMSCs and dermal skins. Results: Based on the results obtained for the three most stable RGs from geNorm (TBP, RPL4, and H2A), Normfinder (TBP, RPL4, and SDHA), and Bestkeeper (TBP, RPL4, and SDHA), it was comprehensively determined that TBP and RPL4 were the most stable RGs in bBMMSCs. However, traditional RGs were suggested to be the least stable (18S) or moderately stable (GAPDH and ACTB) in bBMMSCs. Normalization of FABP4 or ON against TBP, RPL4, and 18S presented significant differences during differentiation of bBMMSCs. However, although significantly low expression of OCT4 was detected in dermal skins compared to that in bBMMSCs when TBP and RPL4 were used in normalization, normalization against 18S exhibited no significance. Conclusion: This study proposes that TBP and RPL4 were suitable as stable RGs for qPCR study in bovine MSCs.

• Journal of Ginseng Research
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• v.42 no.4
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• pp.485-495
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• 2018

Background: The geographical origin of Panax ginseng Meyer, a valuable medicinal plant, is important to both ginseng producers and consumers in the context of economic profit and human health benefits. We, therefore, aimed to discriminate between the cultivation regions of ginseng using the stable isotope ratios of C, N, O, and S, which are abundant bioelements in living organisms. Methods: Six Korean ginseng cultivars (3-yr-old roots) were collected from five different regions in Korea. The C, N, O, and S stable isotope ratios in ginseng roots were measured by isotope ratio mass spectrometry, and then these isotope ratio profiles were statistically analyzed using chemometrics. Results: The various isotope ratios found in P. ginseng roots were significantly influenced by region, cultivar, and the interactions between these two factors ($p{\leq}0.001$). The variation in ${\delta}^{15}N$ and ${\delta}^{13}C$ in ginseng roots was significant for discriminating between different ginseng cultivation regions, and ${\delta}^{18}O$ and ${\delta}^{34}S$ were also affected by both altitude and proximity to coastal areas. Chemometric model results tested in this study provided discrimination between the majority of different cultivation regions. Based on the external validation, this chemometric model also showed good model performance ($R^2=0.853$ and $Q^2=0.738$). Conclusion: Our case study elucidates the variation of C, N, O, and S stable isotope ratios in ginseng root depending on cultivation region. Hence, the analysis of stable isotope ratios is a suitable tool for discrimination between the regional origins of ginseng samples from Korea, with potential application to other countries.

• Journal of The Korean Society of Integrative Medicine
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• v.1 no.2
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• pp.93-104
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• 2013

Purpose : The purpose of this study is balanced exercise program according to the supporting surface any affect women's ability to balance high heels. Methods : This study subjects were normal 20 females in their twenties. They were divided into stable supporting surface(n=10), unstable supporting surface(n=10), and they exercised three times a week for 5 weeks. In order to compare the difference of balance, the subjects were measured before and after training. Balance was examined using the balance master 7.0 version systems. Results : 1. There was significant difference values between pre-exercise and post-exercise of unstable surface group on the FORM-EO and FORM EC of MCTSIB and all of Unilateral Stance variables. 2. There was significant difference values between pre-exercise and post-exercise of stable surface group on the mean EPE, mean MXE, and mean DCL of LOS. 3. There was significant difference values between pre-exercise and post-exercise of unstable surface group of the rhythmic weight shift. 4. There was significant difference value in the slow directional control of rhythmic weight shift between groups after exercise. Conclusion : Training on stable supporting surface group and unstable supporting surface group improve balance.

• Korean Journal of Plant Resources
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• v.20 no.6
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• pp.504-510
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• 2007

GE interaction is the expression of differential genotypic adaptation across environments. GE interactions through different stability parameters and performance of the traits of genotypes were studied. The traits were days to maturity, pod length, number of pods/ plant, 100-seed weight and seed yield/plant in ten soybean genotypes across five environments. Significant differences were observed for genotypes, environments and GE interactions. Stability analysis after Eberhart and Russell's model suggested that the genotypes used in this study were all more or less responsive to environmental changes. Most of the genotypes perform better in Env.3. Based on phenotypic indices(Pi), regression ($S^2di$) genotype Garurab was found fairly stable for days to maturity. BS-23 and G-2120 may be considered as stable genotype for pod length. All the genotypes except G-2120 showed that the genotypes were relatively unstable under environmental fluctuation for the number of pod/plant. Genotype BS-23 was found most stable among all the genotypes for 100-seed weight. BS-3 and Gaurab was the most stable and desirable genotypes for seed yield in soybean.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.1060-1067
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• 2005

This paper attempts to derive and analyze the dynamic system of pinching a rigid object by means of two multi-degrees-of-freedom robot fingers with soft and deformable tips. It is shown firstly that a set of differential equation describing dynamics system of the manipulators and object together with geometric constraint of tight area-contacts is formulated by Lagrange's equation. It is shown secondly that the problems of controlling both the forces of pressing object and the rotation angle of the object under the geometric constraints are discussed. In this paper, the control method for dynamic stable grasping and enhancing dexterity in manipulating things is proposed. It is illustrated by computer simulation that the control system gives the performance improvement in the dynamic stable grasping of the dual fingers robot with soft tips.

• Korean Journal of Applied Biomechanics
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• v.20 no.3
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• pp.311-318
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• 2010

This study examined the double piked dismount among the landing techniques of parallel bars based on three-dimensional motion analysis. Four male national gymnasts were the subjects. This study was performed to provide quantitative data highlighting players strengths and weaknesses to enable more stable landing technique. The variables analyzed were the position and velocity of center of gravity(CG) and angles of shoulder joints, hip joints, and trunk. The results are as follows: S1 secured the height of flight with fast vertical rise. After the easy spin in the air, he conducted a stable landing maintaining a proper hip joints angle. S2, S3, and S4, however, began the backward somersault already before leaving the bars, so they moved backward greatly making it more difficult to achieve a higher flight path. As a result, they couldn't control the velocity of their backward movement at landing. For a stable landing, they have to maintain the negative shoulder angle when rising, minimize both antero-posterioror side-to-side movements by doing a strong tap using hip joints, to secure the height of flight before the somersault. Results also show that at the descent, they should conduct rapid spinning by increasing their shoulder and hip joints to the maximum while controlling their velocity.

• Physical Therapy Rehabilitation Science
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• v.11 no.4
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• pp.579-584
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• 2022

Objective: To identify the effect of unstable surface kettlebell exercises on muscle activity and balance in middle and high school baseball players. Design: Randomized controlled trial. Methods: The participants were 29 middle and high school baseball players (Unstable surface kettlebell exercises group, USKE: 15; Stable surface kettlebell exercise group, SSKE: 14). The players in the USKE group performed kettlebell exercises for 60 mins on a stable surface thrice a week for 6 weeks, and the return rack was solidified on a stable surface in the same way as the experimental group. Before and after the experiment, the participant's muscle activity of the tibialis anterior and soleus was measured. In addition, the participant's balance was evaluated by measuring the distance moved from the foot's center of pressure (COP). The paired t-test was used to compare groups before and after the experiment. The independent t-test was conducted to assess differences in the degree of change between the two groups before and after the experiment. Results: Compared to the SSKE group, the USKE group showed significant differences in the muscle activity of the tibialis anterior and soleus and the balance with the eyes open and closed. Conclusions: The players in the USKE group showed a significant difference in muscle activity and balance than in those in the SSKE group. Thus, effectively including an unstable surface in the kettlebell exercise program will help improve physical function in athletes and people with musculoskeletal disorders.

• The korean journal of orthodontics
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• v.27 no.5 s.64
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• pp.669-682
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• 1997

[$Bj\ddot{o}rk$] and Skieller concluded after following facial growth patterns using implants in upper and lower jaw bones that stable structures, which are not influenced by the growth do exist and so these must be the reference landmarks in the process of superimposition. In spite of such facts, for the last 40 years since the discovery of stable structures, most orthodontists have preferred best fit superimposition method to structural method. Cases such as Angle's Class II division 2 malocclusion that show characteristic forward and upward growth or one under long period of post-treatment observation demonstrate that distinct differences exist between the two methods of superimposition. Today, ethical concerns prohibit further growth studies that use implants, md so there is no choice but to use stable structural superimposition method based on $Bj\ddot{o}rk's$ data. Thus, to encourage clinical use of stable structural superimposition method, logic for the use of stable structural superimposition method will be demonstrated, and its technical methods of application will be introduced step by step.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.4
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• pp.419-427
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• 2023

This study, analyzed the stomach contents, stable isotope signatures (δ¹³C and δ¹⁵N) in muscle tissue, and potential food sources (particulate organic matter, Zostera marina, epiphytes, and zooplankton) of Sardinops melanostictus in the southern coast of Korea to evaluate its dietary changes across seasons (summer and autumn 2022). The diet of S. melanostictus predominantly comprised organic detritus (99%) regardless of the season. The isotope results showed that S. melanostictus had mean δ¹³C and δ¹⁵N values of -17.7±0.4‰ and 10.6±0.5‰, respectively and seasonal variations were observed (t-test, P=0.010, P=0.002). However, the isotopic mixing model showed that particulate organic matter was a major nutritional source for S. melanostictus in summer and autumn (88.7% and 90.2%, respectively). Based on the stomach contents and stable isotope signatures, these results may help improve the understanding of S. melanostictus and establish the management measures for their conservation.

• Journal of Korea Water Resources Association
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• v.51 no.3
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• pp.183-193
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• 2018

Channel-forming discharge for downstream section of Yeongju dam in Naesung stream was calculated to analyze stable channel geometry. Determined channel-forming discharge was applied to design stable channel slope, depth, and base width at Yonghyeol station. Used data for channel-forming discharge and stable channel analysis were collected in downstream section of Yeongju dam in Naesung stream before the dam construction. Specified recurrence interval discharge, effective discharge, and bankfull discharge were analyzed and compared to decide final channel-forming discharge which was $260m^3/s$ of bankfull discharge. Stable channel analysis and design program was applied to predict stable channel section of width, depth, and slope with various sediment transport equations of Ackers and White, Brownlie, Engelund and Hansen, and Yang's equations. As a result, all equations of sediment transport produced milder slopes compared to current bed slope of 0.00177 and Ackers and White equation presented the most similar flow depth of current section with the design constraint of current channel width.