• Title/Summary/Keyword: S/Z ratio

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Evaluation of Natural Attenuation by Addition of Fumarate as Carbon Source and Gene Analysis in Groundwater Sample (지하수 중 탄소원으로 fumarate 주입과 유전자분석을 통한 질산성질소 자연저감도 평가)

  • Park, Sunhwa;Kim, Hyun-Gu;Kim, Sohyun;Lee, Min-Kyeong;Lee, Gyeong-Mi;Kim, Young;Kim, Moon-Su;Kim, Taeseung
    • Journal of Soil and Groundwater Environment
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    • v.19 no.4
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    • pp.62-69
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    • 2014
  • In the results of monitoring nitrate concentration in more than 8,000 groundwater wells around agro-livestock, the average and maximum nitrate concentration was 9.4 mg/L and 101.2 mg/L, respectively. Since about 31% of the monitoring wells was exceed the quality standard for drinking water, nitrate control such as remediation or source regulation is required to conserve safe-groundwater in South Korea. Typical nitrate-treatment technologies include ion exchange, reverse osmosis, and biological denitrification. Among the treatment methods, biological denitrification by indigenous microorganism has environmental and economic advantages for the complete elimination of nitrate because of lower operating costs compared to other methods. Major mechanism of the process is microbial reduction of nitrate to nitrite and nitrogen gas. Three functional genes (nosZ, nirK, nirS) that encode for the enzyme involved in the pathway. In this work, we tried to develop simple process to determine possibility of natural denitrification reaction by monitoring the functional gene. For the work, the functional genes in nitrate-contaminated groundwater were monitored by using PCR with specific target primers. In the result, functional genes (nosZ and nirK) encoding denitrification enzymes were detected in the groundwater samples. This method can help to determine the possibility of natural-nitrate degradation in target groundwater wells without multiplex experimental process. In addition, for field-remediation application we selected nitrate-contaminated site where 200~600 mg/L of nitrate is continuously detected. To determine the possibility of nitrate-degradation by stimulated-natural attenuation, groundwater was sampled in two different wells of the site and nitrate concentration of the samples was 300 mg/L and 616 mg/L, respectively. Fumarate for different C/N ratio was added into microcosm bottles containing the groundwater to examine denitrification rate depending on carbon concentration. In the result, once 1.5 times more than amount of fumarate stoichiometry required was added, the 616 mg/L of nitrate and 300 mg/L of nitrate were completely degraded in 8 days and 30 days. The nitrite, byproduct of denitrification process, was also completely degraded during the experimental period.

Growth Performance and Photosynthesis of Two Deciduous Hardwood Species under Different Irrigation Period Treatments in a Container Nursery System (시설양묘과정에서 관수 주기 처리에 따른 두 활엽수종의 생장 및 광합성 기구 변화)

  • Cho, Min-Seok;Lee, Soo-Won;Hwang, Jae-Hong;Kim, Jae-Won
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.14 no.1
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    • pp.28-38
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    • 2012
  • This study was conducted to investigate growth performance, photosynthesis, water use efficiency (WUE), and stomatal conductance ($g_s$) of container seedlings of Liriodendron tulipifera and Zelkova serrata growing under three different irrigation periods (1 time/1 day, 1 time/2 days and 1 time/3 days) for high seedling quality. The root collar diameter and height of L. tulipifera and Z. serrata seedlings were highest with 1 time/1 day irrigation, whereas they were lowest with 1 time/3 days irrigation. The two species showed low drought tolerance. As irrigation period was shortened, biomass and seedling quality index (SQI) of the two species increased. The ratio of height to root collar diameter (H/D) and the ratio of below to aboveground biomass (T/R) of the two species were lower with 1 time/3 days than at other irrigation periods. L. tulipifera and Z. serrata seedlings showed significantly higher photosynthetic capacity with 1 time/1 day irrigation. As irrigation period was shortened, $g_s$ of two species increased, while their WUE decreased significantly (P<0.05) These results show that 1 time/1 day irrigation provides the most optimal water condition for container seedling production of two species and irrigation controlling is very important for growth and quality of container seedlings.

Effect of Dietary Supplementation of Fresh Water Algae Euglena on the Performance and Fatty Acid Composition of Breast Muscle of Broiler Chickens (육계에서 담수녹조류 Euglena 첨가사료가 생산성 및 흉근의 지방산 조성에 미치는 영향)

  • Choi S. W.;Park I. K.;Park B. S.
    • Korean Journal of Poultry Science
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    • v.31 no.4
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    • pp.273-281
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    • 2004
  • A feeding trial was conducted with Euglena strains grown under different media. The effect of supplementation of Euglena on the performance, nutrient availability and fatty acid composition of breast muscle was studied. In experiment I, two hundred ten hatched broiler chicks (Ross) were assigned to seven dietary treatments for 5 weeks. Each treatment consisted of 3 replications with 10 birds each. Control diet was formulated to have $22\%$ CP and 3,150 kcal ME/kg for starter diet, $19\%$ CP and 3,200 kcal ME/kg for finisher diet. Euglena gracilis Z. (EG) was added to control diet at the plevel of 0.25, 0.5, $1.0\%$ and Euglena gracilis Z. bleached and DHA enriched (EGBD; a strain mutated by streptomycin and cultivated in DHA enriched medium) at the level of 0.5, 1.0, $2.0\%$ in the diet. In experiment 2, two hundred fifty hatched broiler chicks (Ross) were assigned to five dietary treatments: T1; Control, T2; T1 + Euglena gracilis Z. DHA enriched (EGD; cultivated in DHA enriched medium) $0.5\%$, T3; T1 + EGD $1.0\%$, T4; T1 + EGBD $0.5\%$, T5; T1 + EGBD $1.0\%$. The weight gain and feed consumption were measured weekly. Fatty acid composition of breast muscle was determined. In experiments I and 2, Euglena supplementation had no significant effects on weight gain, feed intake and feed conversion ratio. In experiment 1, EGBD treatments significantly increased DHA concentration but decreased concentration of linoleic acid and arachidonic acid in breast muscle. EGBD 2% treatment showed the highest DHA concentration (14.27%) which is 3.9 times of that of the control ($3.66\%$). In experiment 2, $1.0\%$ EGBD treatment showed highest EPA, lignoceric acid and DHA level in breast muscle (P<0.05). Also, EGD treatments significantly increased DHA and EPA concentration. It was concluded that EGBD and EGD can be supplemented to broiler diet to produce DHA enriched broiler meat.

Wind direction field under the influence of topography, part I: A descriptive model

  • Weerasuriya, A.U.;Hu, Z.Z.;Li, S.W.;Tse, K.T.
    • Wind and Structures
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    • v.22 no.4
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    • pp.455-476
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    • 2016
  • In both structural and environmental wind engineering, the vertical variation of wind direction is important as it impacts both the torsional response of the high-rise building and the pedestrian level wind environment. In order to systematically investigate the vertical variation of wind directions (i.e., the so-called 'twist effect') induced by hills with idealized geometries, a series of wind-tunnel tests was conducted. The length-to-width aspect ratios of the hill models were 1/3, 1/2, 1, 2 and 3, and the measurements of both wind speeds and directions were taken on a three-dimensional grid system. From the wind-tunnel tests, it has been found that the direction changes and most prominent at the half height of the hill. On the other hand, the characteristic length of the direction change, has been found to increase when moving from the windward zone into the wake. Based on the wind-tunnel measurements, a descriptive model is proposed to calculate both the horizontal and vertical variations of wind directions. Preliminarily validated against the wind-tunnel measurements, the proposed model has been found to be acceptable to describe the direction changes induced by an idealized hill with an aspect ratio close to 1. For the hills with aspect ratios less than 1, while the description of the vertical variation is still valid, the horizontal description proposed by the model has been found unfit.

A Study on ZigBee-Based Routing Algorithm (스마트그리드를 위한 지그비 기반의 라우팅 알고리즘에 관한 연구)

  • Kang, Hyung-Seok;Im, Song-Bin;Oh, Young-Hwan
    • Journal of the Korea Society of Computer and Information
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    • v.17 no.12
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    • pp.137-148
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    • 2012
  • In this paper, we proposed the location-aware coordinate routing algorithm for improving the performance of routing algorithm by using ZigBee in Smart Grid environment. A distributed address allocation scheme used an existing algorithm that has wasted in address space. The x, y and z coordinate axes from divided address space of 16 bit to solve this problems. However, coordinate routing does not take account of wireless link condition. If wiress link condition is not considered, when the routing table is updated, the nodes with bad link conditions are updated in the routing table and can be chosen as the next hop. This brings out the retransmissions because of received packet's errors. Also, because of these retransmissions, additional power is consumed. In this paper, we propose the location-aware coordinate routing algorithm considering wiress link condition, where reliable data transmission is made and the consumed enegy is minimize. and we compared and connected region and transition region of ZigBee location based routing in the aspect of average number of multi hops, subordinate packet delivery ratio, delay time, and energy consumption of proposed algorithm. It turned out that there were improvements in performances of each items.

The Calculation Method of Apparent Earth Pressure in Multi-Layered Ground with Clay and Sand (점토와 모래가 포함된 다층지반의 경험토압 산정방법에 관한 연구)

  • Kim, Byung-Il;Hong, Kang-Han;Kim, Jin-Hae;Han, Sang-Jae
    • Journal of the Korean Geosynthetics Society
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    • v.20 no.1
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    • pp.21-34
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    • 2021
  • In this study, to solve a problem that cannot consider the contribution effect of each layers when the apparent earth pressure in homogeneous ground is applied to multi-layered ground, the measured earth pressures at World were investigated and analyzed. It has been confirmed that the apparent earth pressure in mulit-layered ground is different from single ground and that the extra layer's contribution to the earth pressure cannot be considered. The conventional method of calculating the apparent earth pressure for single ground was extended to mulit-layered ground, and proposed and verified the applicable method for both single and mulit-layered ground. The proposed methods predicted the earth pressure closer to the measurements at the excavation depth of 0.1Z/H or below, and the prediction reliability was evaluated to be better than the conventional method. Among the proposed methods, the method of considering the area ratio of the active failure has a geotechnical validity and predicts the most similar results to the actual earth pressure. To confirm the applicability of the proposed methods, it was presented by comparing and analyzing the results of the proposed methods with the conventional method for the actual case.

A comprehensive review of the Fenton-based approaches focusing on landfill leachate treatment

  • Hussain, Mujtaba;Mahtab, Mohd Salim;Farooqi, Izharul Haq
    • Advances in environmental research
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    • v.10 no.1
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    • pp.59-86
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    • 2021
  • Landfilling is the most commonly adopted method for a large quantity of waste disposal. But, the main concern related to landfills is the generation of leachate. The leachate is high strength wastewater that is usually characterized by the presence of high molecular recalcitrant organics. Several conventional methods are adopted for leachate treatment. However, these methods are only suitable for young leachate, having high biodegradability and low toxicity levels. The mature and stabilized leachate needs advanced technologies for its effective treatment. Advanced oxidation processes (AOPs) are very suitable for such complex wastewater treatment as reported in the literature. After going through the literature survey, it can be concluded that Fenton-based approaches are effective for the treatment of various high/low strength wastewaters treatment. The applications of the Fenton-based approaches are widely adopted and well recognized due to their simplicity, cost-effectiveness, and reliability for the reduction of high chemical oxygen demand (COD) as reported in several studies. Besides, the process is relatively economical due to fewer chemical, non-sophisticated instruments, and low energy requirements. In this review, the conventional and advanced Fenton's approaches are explained with their detailed reaction mechanisms and applications for landfill leachate treatment. The effect of influencing factors like pH, the dosage of chemicals, nature of reaction matrix, and reagent ratio on the treatment efficiencies are also emphasized. Furthermore, the discussion regarding the reduction of chemical oxygen demand (COD) and color, increase in biodegradability, removal of humic acids from leachate, combined processes, and the pre/post-treatment options are highlighted. The scope of future studies is summarized to attain sustainable solutions for restrictions associated with these methods for effective leachate treatment.

Risk Factors and Clinical Outcomes of Unplanned Reintubation after Planned Extubation in Adult Patients admitted to the Intensive Care Unit after Cardiac Surgery (성인 심장수술 후 중환자실에 입실한 환자의 계획된 발관 후 비계획적 기관 재삽관 위험요인과 임상결과)

  • Lee, Ju-Hee;Choi, Hye-Ran
    • Journal of Korean Critical Care Nursing
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    • v.15 no.3
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    • pp.88-100
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    • 2022
  • Purpose : This study aimed to identify risk factors for unplanned reintubation after planned extubation and to analyze the clinical outcomes in patients admitted to the intensive care unit after cardiac surgery. Methods : The study examined patients who underwent intubation and planned extubation admitted to the intensive care unit after cardiac surgery between January 1, 2017, and December 31, 2021. The reintubation group comprised 58 patients underwent unplanned reintubation within 7 days of planned extubation. The maintenance group comprised 116 patients who did not undergo reintubation and were matched with the reintubation group using the rational for matching criteria. Data were collected retrospectively from electronic medical records. We used the independent t-test, Mann-Whitney U test, 𝑥2-test, Fisher's exact test, and logistic regression analysis with SPSS/WIN 27.0. Results : The multivariate logistic regression analysis demonstrated that albumin (odds ratio [OR]=0.38, 95% confidence interval [CI]=0.20-0.72), surgery time (OR=1.54, 95% CI=1.20-1.97), PaO2 before extubation (OR=0.85 per 10 mmHg, 95% CI=0.75-0.97), postoperative arrhythmia (OR=2.82, 95% CI=1.22-6.51), reoperation due to bleeding (OR=4.65, 95% CI=1.27-17.07), and postoperative acute renal failure (OR=2.97, 95% CI=1.09-8.04) were risk factors for unplanned reintubation. The reintubation group had a higher in-hospital mortality rate (𝑥2=33.74, p<.001), longer intensive care unit stay (Z=-7.81, p<.001), and longer hospital stay than the maintenance group (Z=-8.29, p<.001). Conclusion : These results identified risk factors and clinical outcomes of unplanned reintubation after planned extubation after cardiac surgery. These findings should be considered when developing and managing an intervention program to prevent and reduce the incidence of unplanned reintubation.

Heterotic Expression in Silk Productivity of Newly Evolved Thermo-tolerant Bivoltine Hybrids of Silkworm Bombyx mori L.

  • Rao, P.Sudhakara;Datta, R.K.;Vijaya Kumari, K.M.;Palit, A.K.;Haque Rufaie, S.Z.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.9 no.1
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    • pp.53-58
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    • 2004
  • Studies on heterotic expression in the silk productivity of 66 thermo-tolerant bivoltine hybrids reared under high temperature $(36{\times}1^{\circ}C)$ and low humidity (60${\times}$5%) conditions revealed that manifestation of heterosis was highly significant for majority of the quantitative traits contributing to more cocoon productivity. Observations were made on 8 economically important traits such as fecundity, pupation rate, cocoon yield, cocoon weight, cocoon shell weight, cocoon shell ratio, cocoon filament length and raw silk. Analysis of variance results revealed great deal of variability indicating significant (P < 0.001) variations. Out of 66 hybrids, 7 hybrids exhibited positive heterosis for seven traits and 4 hybrids expressed significant hetero-beltiosis for 6 traits, out of 8 traits evaluated. Based on results, the hybrids ${SR_1} {\times} {SR_5}and {CSR_2} {\times} {SR_5}$ are adjudged as most promising ones and can be exploited commercially in tropical climates to increase the cocoon productivity.

Cytotoxicity of Cytosine Deaminase (CD) Adenoviral Vectors(AV) with a Promoter (L-plastin) for Epithelial Cancer Cells.

  • Chung, Injae;Jung, Kihwa;Deisseroth, Albert B.
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1997.04a
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    • pp.80-80
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    • 1997
  • The object of this study was to develop a gene therapy strategy for ovarian cancer. We have previously shown that AV with a L-plastin (LP) promoter infects breast and ovarian cancer cells and expressed ${\beta}$-galactosidase cDNA in preference to normal fibroblast cells and hematopoietic cells. We now report on the cytotoxicity of Ad.LP.CD, an AV carrying a CD cDNA which converts the pro-drug, 5-Fluorocytosine (5-FC) into the toxic drug 5-Fluorouracil (5-FU). Infection of Ad.LP.CD into either 293 cells or ovarian cancer cells generated the functional CD as measured by HPLC analysis. Using a ratio of AV to OVCAR3 cell of 100 and a 5-FC concentration of 100 ${\mu}$M, we achieve an over 95 % of cell growth inhibition. We are using flow cytometry analysis for ${\beta}$ -galactosidase and ovarian cancer associated folate receptor to screen primary ascites samples for infectivity after infection with an adenoviral vector, i.e., Ad.LP.LacZ. This vector system may be of value in the treatment of microscopic disease of ovarian cancer in the peritoneal cavity.

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