• Journal of Aquaculture
• /
• v.10 no.4
• /
• pp.403-407
• /
• 1997

Intraspecific sequence variation was detected by polymerase chain reaction (PCR) and direct sequencing of a 350-nucleotide region of the mitochondrial 12S rRNA gene of two natural populations (Han River and Nakdong River) and one hatchery stock (Jinhae Inland Fisheries Institute) of local strain common carp, one Israeli strain of common carp stock from Pukyong National University (PKU), and one hybrid between Israeli strain of common carp female and local strain common carp male from PKU stock. There is little variation in 350 bases of the mitochondrial 12S rRNA gene sequences among 2 natural and 1 hatchery local strain common carp populatins, representing abut 7 to 20 nucleotide differences (less than 6%). The sequence of specimens from Han River was more similar to that from Nakdong River (identity=98.0%) than to that from Jinhae Inland Fisheries Institute (identity=96.3%). Sequence variation between Israeli strain and wild local strain common carp was higher than the variation within natural stocks. The level of variation was ranged from 15.7 to 17.7%. The hybrid showed very similar nucleotide4 sequence of 12S rRNA gene to the sequence of Israeli strain with the identity of 98.9%.

• Journal of the military operations research society of Korea
• /
• v.35 no.2
• /
• pp.63-76
• /
• 2009

The rapid development of IT and Science & Technology has greatly influenced the aspect of future warfare. Accordingly, the Army Forces require efficient logistics support, hence the importance of AS/RS has steadily increased. This research validated mathematical propriety of the management problem of S/R machine in AS/RS with mathematical analysis based on its travel time and simulation analysis using Automod. After all, we could improve the performance of S/R machine based on its dwell point and turn-over distribution.

• Journal of Korean society of Dental Hygiene
• /
• v.13 no.5
• /
• pp.889-900
• /
• 2013

Objectives : Molecular biology techniques were employed to assess diversity of bacterial in children's dental caries. Methods : DNA of germs was extracted and the diversity of the 16S rRNA clones was analyzed by amplified rDNA restriction analysis and sequencing. The experimental samples were pit and fissure caries (PC), deep dentinal caries (DC), smooth surface caries (SC), and supragingival plaque (PQ) from 50 children of age less than 12 years old. The control group was healthy teeth supragingival plaque (HT). Thirty clones from each 16S rRNA clone library of 5 samples were randomly selected, thus a total of 150 clones were analyzed. Results : Amplified rDNA restriction analysis uncovered 18, 20, 11, 17, and 22 phylotypes from healthy teeth, pit and fissure caries, deep dentinal caries, smooth surface caries, and supragingival plaque, respectively. Sequencing analysis found the dominance of Actinomycs naeslundii and Fusobacterium nucleatum in the healthy teeth; Leptotrichia sp. in the pit and fissure caries; Actinomyces sp., Streptococcus mutans, and Rahnella aquatilis in the deep dentinal caries; Streptococcus mutans and Actinomyces sp. in the smooth surface caries; Enterobacter hormaechei and Streptococcus sanguinis in the supragingival plaque. Conclusions : Clonal analysis identified 6 phyla, 20 genera, and 51 species.

• Animal Systematics, Evolution and Diversity
• /
• v.16 no.2
• /
• pp.203-211
• /
• 2000

Partial mitochondrial 16S rDNA and COI gene were amplified using PCR and sequenced for four species of oysters in Korea. Phylogenetic relationships among them were inferred from their aligned sequences by neighbor-joining method. The sequence comparison data of two mitochondrial genes showed that the genetic distinction between two oyster genera (Crassostreo and Ostrea) was obvious. Phylogenetic analysis based on the nucleotide sequences and A+T percentage of two genes indicates that C. gigas and C. nippona strongly formed a sister group and then C. ariakensis was clustered with the clade although that based on amino acid sequences of COI gene by neighbor-joining method represented different phylogenetic tree.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2016.05a
• /
• pp.218-218
• /
• 2016

차량용 강우 센서는 강우측정이 어려운 지역의 강수량 측정이 가능하고, 실시간으로 강우정보를 생성해 내기 때문에 차세대 강우측정기로써 전망이 기대된다. 차량용 강우 센서는 일반적인 강우관측기와 달리 물 입자가 커질수록 빛의 산란이 크게 일어나는 현상을 이용한다. 산란이 크게 일어나면 강우 센서에 입력되는 값이 줄고 이는 강수가 높다는 것을 의미한다. 강우가 발생하면 자동차 전면 유리창에 부착된 강우 센서가 감지하는 우적량을 강우량으로 환산하는 방법을 통해 강우량을 산정한다. 강우가 쌓이고 나서 나중에 그 값을 측정하는 것이 아니라 즉시 그 값을 계산하여 강우량을 산출해야하기 때문에 단계가 복잡하다. 수식이 복잡할수록 오차가 발생할 확률도 크고 처리 속도도 느려지기 때문에 W-S-R 관계식을 이용하여 복잡한 수식을 간단하게 정리할 필요가 있다. AWS 기상관측소와의 비교 분석을 위해 차량에 우량계를 장착하였으며 W-S-R 관계식을 통하여 상관관계 분석하여 회귀식을 도출 한다. W-S-R 관계식이라 함은 와이퍼의 속도관계(W), 강우센서(S), 실제 강우(R)을 의미한다. 여기서 와이퍼의 속도관계는 와이퍼의 한 번 이동 했을 시 실제 강우는 실내강우발생 장비를 제작하여 10~80mm의 강우를 발생시키고 그 값은 우량계로 관측한다. 본 연구에서는 물 입자의 산란과 차량용 강우 센서 간의 관계식 도출과 W-S-R 관계식을 이용하여 실제 강우 정보와의 상관관계를 위한 분석을 통한 회귀식 개발을 목표로 한다.

• Animal Systematics, Evolution and Diversity
• /
• v.37 no.3
• /
• pp.235-241
• /
• 2021

To provide better taxonomic information of the genus Nectoneanthes, the two DNA barcode regions of mitochondrial cytochrome c oxidase subunit I (COI) and 16S ribosomal DNA (rDNA) sequences of Nectoneanthes oxypoda and N. uchiwa were determined. In addition, the respective sequences of four nereidid species closely related to Nectoneanthes were retrieved from GenBank for comparison and to estimate intra- and inter-specific genetic distances. The aligned sequence lengths of COI and 16S rDNA were 570 bp and 419 bp long, respectively. The mean intraspecific variation in both markers was less than 1% in all species except for that in COI of H. diadroma (1.87%). The mean interspecific variation between N. oxypoda and N. uchiwa was 12.02% regarding COI and 1.85% regarding 16S rDNA. In contrast, the mean interspecific variation between species of other genera was comparably higher(i.e., genus Perinereis: 20.5% in COI and 8.3% in 16S rDNA; genus Hediste: 13.18% in COI and 2.64% in 16S rDNA), compared with that between the two Nectoneanthes species. This result indicated that these Nectoneanthes species are genetically more closely related than other congeneric species of different genera. The DNA barcoding information on Nectoneanthes species generated in this study provides valuable insights for further biodiversity studies on nereidid species.

• Journal of Species Research
• /
• v.11 no.4
• /
• pp.321-329
• /
• 2022

Four species of cyanobacteria that are unrecorded in Korea were isolated from freshwater and brackish water. These four species are Laspinema thermale of Laspinemaceae, Planktothricoides raciborskii and Planktothrix spiroides of Microcoleaceae, and Cephalothrix lacustris of Phormidiaceae, all belonging to the order Oscillatoriales. Laspinema thermale is morphologically characterized as apical cells that are longer than other cells. In this strain, the similarity of the 16S rRNA gene sequence with the previously reported L. thermale strains were 99.30-99.50%. Planktothricoides raciborskii, which is characterized by bluntly conical morphology of apical cells, showed 98.80-99.50% of similarity of the 16S rRNA gene sequence to the previously reported P. raciborskii strains. Planktothrix spiroides are characterized by floating due to gas vacuoles. In this strain, the similarity of the 16S rRNA gene sequence with the previously reported P. spiroides strains were 99.80-99.90%. Cephalothrix lacustris, characterized by having calyptra in apical cells, showed 99.80-99.90% similarity of the 16S rRNA gene sequence to previously reported C. lacustris strains. Also, these species were clustered in the same clade in phylogenetic analysis using 16S rRNA gene sequences with each corresponding species.

• Journal of Intelligence and Information Systems
• /
• v.29 no.2
• /
• pp.303-321
• /
• 2023

Metaverse shopping has emerged as a new phenomenon in social commerce. This study aims to investigate the user experience of metaverse stores shopping based on the S-O-R model. The results of the study show that telepresence, entertainment, personalized recommendation, and social interaction have significant positive effects on flow experience and satisfaction in metaverse shopping. Additionally, satisfaction and flow experience are shown to have significant positive effects on user intentions. This study provides valuable implications for the design and management of metaverse stores to improve user experience and increase user intention.

• Journal of muscle and joint health
• /
• v.31 no.2
• /
• pp.117-125
• /
• 2024

Purpose: This study was intended to evaluate the effect of nurse image, satisfaction with one's major and career decision-making self-efficacy on career preparation behavior of first-year nursing students. Methods: A total of 134 first-year nursing students were enrolled in this study. Data were collected from March 25 to April 5, 2024. Data collected prospectively were analyzed by t-test, ANOVA, Pearson's correlation coefficients, and multiple regression analysis. Results: Career preparation behavior showed a significantly positive correlation with nurse image (r=.28, p=.001), satisfaction with one's major (r=.41, p<.001) and career decision-making self-efficacy(r=.61, p<.001). Career decisionmaking self-efficacy showed a significantly positive correlation with nurse image (r=.59, p<.001), satisfaction with one's major (r=.70, p<.001). Satisfaction with one's major showed a significantly positive correlation with nurse image (r=.66, p<.001). Career preparation behavior was significantly affected by nurse in the family and relatives (β=-.21, p=.003) and career decision-making self-efficacy (β=.64, p<.001), which had an explanatory power of 41.0%. Conclusion: To establish career preparation behavior in first-year nursing students, it is necessary to develop a variety of systematic educational programs to enhance career decision-making self-efficacy.

• Bulletin of the Korean Mathematical Society
• /
• v.44 no.3
• /
• pp.483-492
• /
• 2007

In this paper, we study the map projections from pseudo-sphere $S_1^2$ onto the non-lightlike surfaces in the 3-dimensional Lorentzian space, $L^3$, with curvature zero. We show geometrical means and properties of $\mathbb{R}{\times}S_1^1-cylindrical$, $S^1{\times}L-cylindrical$ and $\mathbb{R}{\times}H_0^1-cylindrical$ projections defined on $S_1^2$ to cylinders $\mathbb{R}{\times}S_1^1,\;S^1{\times}L$ and $\mathbb{R}{\times}H_0^1$, respectively, and orthographic and stereographic projections on $S_1^2$ to Lorentzian plane, $L^2$.