• 한국식품영양학회지
• /
• 제25권1호
• /
• pp.123-131
• /
• 2012

The objective of the present study was to evaluate the potential antidiabetic and antioxidant effect of the ethanol extract from Chrysanthemum cornarium L. var. spatiosum(CSE) against alloxan-induced oxidative stress in pancreatic ${\beta}$-cells, HIT-T15. In this study, the antidiabetic effect of CSE was examined using the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliu bromide(MTT) cell proliferation assay, lactate dehydrogenase(LDH) release assay, $NAD^+$/NADH ratio and insulin secretion. To further investigate whether CSE is involved in the antioxidant activity of alloxan-damaged HIT-T15 cells, its antioxidant effect against alloxan-induced oxidative stress was measured in HIT-T15 cells by determining the levels of antioxidant enzymes including superoxide dismutase(SOD), glutathione S-transferase(GST), glutathione reductase(GR) and glutathione peroxidase(GPx). The results of this analysis showed that alloxan significantly decreased cell viability, increased LDH leakage, and lowered $NAD^+$/NADH ratio and insulin secretion in HIT-T15 cells. However, CSE significantly increased the viability of alloxan-treated cells and lowered LDH leakage. The intracellular NAD+/NADH ratio and insulin secretion were also significantly increased by 1.7-fold and 1.3-fold, respectively, after treatment with 100 ${\mu}g/m{\ell}$ CSE. The HIT-T15 cells treated with alloxan showed significant decreases in the activities of antioxidant enzymes, while CSE significantly elevated the levels of antioxidant enzymes. These findings suggest that CSE could have a protective effect against cytotoxicity and dysfunction of pancreatic cells in the presence of alloxan-induced oxidative stress.

• 기술혁신연구
• /
• 제14권3호
• /
• pp.117-154
• /
• 2006

This research examines performance evaluatees' opinions regarding the current institutional performance evaluation systems of Government S&T Research Institutes (GRIs). Under the current evaluation systems, twenty GRIs are grouped into three Research Groups and each Group has its own evaluation systems. One problem of the current institutional evaluation systems is that the systems cannot reflect individual GRIs' characteristics. The following methods are used. First, based on four perspectives of Kaplan & Norton(1992)'s Balanced Scorecard(BSC) model, six perspectives appropriate to GRUs' characteristics are derived. Second, experts classify current performance evaluation measures into the six perspectives. This enables different evaluation systems of three GRI Research Groups to be compared under the same evaluation measures. Third, GRIs' evaluatees are asked to allocate appropriate weights on the performance measures. Evaluatees' weights of a GRI are compared with average weights of the related Group. Finally in every BSC's perspective, GRIs that have extraordinarily over-scored or under-scored weights are analyzed in terms of GRIs' missions, customers, capability of human resources, etc. In the Basic Research Group, the Korea Basic Science Institute is deviated in the financial perspective and the strategic direction perspectives. In the Public Research Group, Korea Institute of Construction Technology is significantly different from other GRIs in three perspectives. Five out of eight GRIs in the Industrial Research Group, GRIs are significantly different each other in several perspectives. It could be concluded that the current institutional evaluation systems are least appropriate in measuring performance of the GRIs of the Industrial Research Group.

• 한국의상디자인학회지
• /
• 제25권3호
• /
• pp.1-16
• /
• 2023

The purposes of this study were to investigate bag purchasing behaviors and design preferences of male and female adult consumers, and to find the differences depending on sex and age variable. A survey was conducted on 400 male and female adults from 20s to 50s. The questionnaire consisted of bag purchase behaviors, bag design preferences, and the subjects' demographic characteristics. The data were analyzed by Cronbach's α, factor analysis, x² test and t-test using SPSS. The results were as follows. First, as bag selection criteria, four factors (practicality, symbolism, aesthetics, and economics) were derived, and adult consumers considered economics as the most important among the factors. As for purchasing information sources, three factors (media, human resources, and store) were derived, and adult consumers considered human resources and store information sources more important than media. The main motive for purchasing bags was age and damage of the owned products, and Internet shopping malls were the most common purchasing place. The average annual cost of purchasing bags was 100,000 to 300,000 won, and the frequency of purchase was about once a year. Second, as bag preference images, four factors (individual, romantic, active, and classic image) were derived, and adult consumers preferred classic images the most. The shoulder bag was the most preferred as the bag shape, and black was the most preferred bag color. For the material, natural leather was the most preferred, and for the size, medium size was the most preferred. Third, bag purchasing behaviors and design preferences showed many significant differences according to the sex and age of the consumers. Therefore, the results of this study suggests that bag companies need to establish product development and marketing strategies in consideration of differences according to the sex and age group of adult consumers.

• Molecules and Cells
• /
• 제28권2호
• /
• pp.111-117
• /
• 2009

The CHRM3 gene is a member of the muscarinic acetylcholine receptor family that plays important roles in the regulation of fundamental physiological functions. The evolutionary mechanism of exon-acquisition and alternative splicing of the CHRM3 gene in relation to transposable elements (TEs) were analyzed using experimental approaches and in silico analysis. Five different transcript variants (T1, T2, T3, T3-1, and T4) derived from three distinct promoter regions (T1: L1HS, T2, T4: original, T3, T3-1: THE1C) were identified. A placenta (T1) and testis (T3 and T3-1)-dominated expression pattern appeared to be controlled by different TEs (L1HS and THE1C) that were integrated into the common ancestor genome during primate evolution. Remarkably, the T1 transcript was formed by the integration event of the human specific L1HS element. Among the 12 different brain regions, the brain stem, olfactory region, and cerebellum showed decreased expression patterns. Evolutionary analysis of splicing sites and alternative splicing suggested that the exon-acquisition event was determined by a selection and conservation mechanism. Furthermore, continuous integration events of transposable elements could produce lineage specific alternative transcripts by providing novel promoters and splicing sites. Taken together, exon-acquisition and alternative splicing events of CHRM3 genes were shown to have occurred through the continuous integration of transposable elements following conservation.

• 성인간호학회지
• /
• 제23권3호
• /
• pp.255-266
• /
• 2011

Purpose: The purpose of this study was to explore nurses' access and use of information resources and to identify the barriers and competency to evidence based practice (EBP). This study used descriptive method to identify baseline data for the purpose of developing strategies for establishing EBP in clinical nursing practice. Methods: Participants in this study were 278 nurses from five hospitals in Daegu and Kyungsangbukdo. The data were collected by self administered questionnaires and SPSS/WIN 15.0 program was used to analyze the data with descriptive statistics, t-test, Pearson's correlation coefficients, and ANOVA. Results: Nurses reported $^*most$ frequently using paper and human resources. The mean score of barriers was $3.02{\pm}0.41$, and competency was $2.70{\pm}0.50$. The reported competency was correlated with nurse factors (r=-.31, p<.001) organization factors (r=-.20, p<.001) and research factors (r=-.12, p<.040) as the barriers to evidence based practice. Conclusion: To promote competency in EBP and to decrease the barriers, it would be necessary to develop the organizational culture that encourages nurses to be involved in research activities. In addition, the development of systemic methods to introduce and establish an education program for facilitating EBP in the clinical settings is needed.

• 한국보건간호학회지
• /
• 제21권2호
• /
• pp.206-216
• /
• 2007

Purpose: The purpose of this study was to investigate the perceived social support of mothers with disabled children. Method: This study was a cross-sectional, descriptive study using a questionnaire survey which included the standardized measure: personal resource Questionnaire 2000. Results: The participants were 98 mothers, each with one with disabled children. Of the 98 participant's children, 39.8% suffered mental retardation, 34.7% was developmentally disability, 16.3% was brain diseases and 9.2% was multiple disabled. The mean age of the disabled children was 8.2 years and of their mothers was 38 years. The order of the life events for which the need help for the subjects required help were 'when anger and frustration arise happened', 'difficulties in human relationship' and 'emergency situations'. The personal resources were spouse (38.1%), friends (20.9%) and parent (12.9%). The mean score of perceived social support was 5.48 out of a total of 7, indicating a slightly high on social support score. Significant differences were found in the level of perceived social support according to the school grade of disabled child's school grade (F=4.04, p= .02) and the mother having a job presence of mothers (t=2.49, p= .01). Conclusion: These findings indicate a need for nursing intervention programs for mothers with disabled children, including anger management, methods of human relationship, support for leisure time and long term illness and providing information.

• 디지털융복합연구
• /
• 제7권1호
• /
• pp.157-164
• /
• 2009

This research focuses on entrepreneurial orientation(EO) of technology spin-offs as they are expected to reduce the gap between their technology and the market. Entrepreneurial orientation is an organizational activity or process that redistributes or combines resources in an innovative way and takes risk to create new values. It helps improve the level of organizational innovation to deal with uncertainty. An empirical study was performed to analyze the EO of spin-offs established by members of university faculties and research institutes in Korea. Antecedent variables to EO are hypothesized to include public policy for university faculty members and researchers at research institutes, the relationship with incubating organizations, and the level of social networking with other firms. The EO and technological performance relationships are also hypothesized. Data from a total of 121 spin-off organizations were collected and a series of multiple regressions were performed. The performance variable included both technological performance, such as the number of newly marketed products and new technology and subjective performance, such as the level of satisfaction with sales amount and profitability. Several important conclusions were drawn from this study. First, while government policy is not related to EO, the policy of a university or a research institute has a significant effect on the level of innovativeness. Second, a high percentage of human resources applied at the incubating organization results in a negative effect on risk taking. The level of cooperation with the incubating organization too is not related to Entrepreneurial Orientation. Third, the intensity of cooperative networking with other firms has a significant effect on risk-taking. Network relationship with government related organizations is not related to Entrepreneurial Orientation. This study analyzes the importance and role of policy of the university and research institute and networking with other firms to improve EO. It also suggests that a high composition of human resources at the incubating organization has a negative relationship with EO.

• 한국축산식품학회지
• /
• 제35권2호
• /
• pp.164-170
• /
• 2015

The objective of this study was to evaluated the photoprotective effects of porcine placenta extract (PPE) on ultraviolet B (UVB)-induced oxidative stress in human keratinocytes (HaCaT) to evaluate its functional activities as a skin food ingredient. PPE prepared by subcritical water extraction was termed SPE, and subsequently digested by enzymes to prepare E-SPE. Increased intracellular reactive oxygen species (ROS) levels (192.0%) induced by UVB were decreased by SPE and E-SPE. SPE had more effective ROS scavenging activity than E-SPE treatment. UVB treatment increased expression of tissue inhibitor of metalloproteinase 1 (TIMP-1), and this elevated expression was decreased by E-SPE treatment. High-dose treatment with E-SPE (50 and 100 µg/mL) reduced TIMP-1 expression levels of UVB-C (control) to 33.5 and 34.6%, respectively. In contrast, at low SPE doses (1 and 10 µg/mL), the treatment slightly decreased TIMP- 1 expression levels to 73.3% and 71.3% of UVB-C, respectively. In conclusion, the present study demonstrated the protective effect of SPE and E-SPE against UVB damage in keratinocytes via ROS scavenging, down-regulating MMP-2 expression and up-regulating TIMP- 1 expression. This highlights the potential for SPE as an ingredient in the preparation of functional food against photoaging.

• 아동학회지
• /
• 제32권6호
• /
• pp.63-79
• /
• 2011

The aim of this study is to examine the pathways from attachment to children's psychological well-being in addition to the mediating role of children's ego-resilience and peer support. The participants, 297 fifth and sixth grade students from Seoul and other cities, completed questionnaires on attachment, ego-resilience, peer support and psychological well-being. Data were analyzed by mean, standard deviation, Pearson's correlation coefficients, factor analysis, and path analysis. The findings are as follows. Attachment didn't appear to directly influence children's psychological well-being, but showed a number of indirect effects in two ways. First, attachment indirectly affected children's psychological well-being by ego-resilience as a simple form of mediation. Second, attachment influenced children's psychological well-being through children's ego-resilience and peer support as a dual mediation. Ego-resilience demonstrated the strongest effect on psychological well-being among all the factors examined. These results highlight the way in which children's personal internal resources play an important role in the pathways from attachment to their psychological well-being.

• 디지털융복합연구
• /
• 제13권11호
• /
• pp.481-491
• /
• 2015

본 연구는 대학의 인성교육을 목표로 개발된 교양교과목의 효과성을 검증하고 결과를 논의함으로써, 향후 개선된 인성교육 교과를 개발하기 위함에 목적이 있다. 연구는 지방의 K대학교의 마음나눔교육부에서 대학의 교육 목표에 따라 개발된 '마음교양' 교양교과를 대상으로 효과성을 검증하였다. 교과는 15주로 진행했으며 구체적인 인성교육의 내용은 '감사'의 가치관 증진으로 구성되었다. 효과성을 검증하기 위해 켄터키 마음챙김기술 척도, 게슈탈트접촉경계진단검사, 나의식우리의식 척도, 자존감 척도를 사용하였다. 연구대상자는 '마음교양' 교양교과를 수강한 학생(실험군)과 수강하지 않은 학생(통제군) 229명의 자료이며, 연구방법은 집단간 차이분석을 하였다. 분석결과, 게슈탈트접촉경계진단검사의 하위요인인 연결성 척도에서만 유의미한 집단간 차이를 보였다(t=2.323, p=.021). 이는 대인관계에서의 연결성 회복을 시사하며, 인성교육의 일부 효과성이 검증되었음을 의미한다. 추후 연구에서는 감사의 마음이외에 K대학교의 교육이념을 반영한 교과 내용을 구체화하고, 교육방법에서도 K여자대학의 교육 목표를 구체적으로 반영한 교과개발이 필요함이 논의되었다.