• International Journal of Computer Science & Network Security
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• 제23권11호
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• pp.149-161
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• 2023

Internet of Things (IoT) is a relatively new concept that has gained immense popularity in a short period of time due to its wide applicability in making human life more convenient and automated. As an illustration: the development of smart homes, smart cities, etc. However, it is also accompanied by a substantial number of risks and flaws. IoT makes use of low-powered devices, so secure, less time-consuming and energy-intensive transmission (routing) of messages due to the limited availability of energy is one of the many and most significant concerns for IoT developers. The following paper presents a trust-based routing scenario for the Internet of Things (IoT) that exploits the past transmission record from the cupcarbon simulator's log files. Artificial Neural Network is used to quantify knowledge of trust, calculate the value of trust, and share this information with other network devices. As a human behavioural pattern, trust provides a superior method for making routing decisions. If there is a tie in the trust values and no other path is available, the remaining battery power is used to break the tie and make a forwarding decision; this is also seen as a more efficient use of the available resources. The proposed algorithm is observed to have superior energy consumption and routing decisions compared to conventional routing algorithms, and it improves the communication pattern.

• The Plant Pathology Journal
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• 제39권1호
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• pp.39-51
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• 2023

Common bean (Phaseolus vulgaris L.) is one of the most important crops in human food production. The occurrence of diseases, such as white mold, caused by Sclerotinia sclerotiorum can limit the production of this legume. The use of Trichoderma has become an important strategy in the suppression of this disease. The aim of the present study was to evaluate the effect of volatile organic compounds (VOCs) emitted by Trichoderma azevedoi CEN1241 in five different growth periods on the severity of white mold in common bean. The in vitro assays were carried out in double-plate and split-plate, and the in vivo assays, through the exposure of the mycelia of S. sclerotiorum to the VOCs of T. azevedoi CEN1241 and subsequent inoculation in bean plants. Chemical analysis by gas chromatography coupled to mass spectrometry detected 37 VOCs produced by T. azevedoi CEN1241, covering six major chemical classes. The profile of VOCs produced by T. azevedoi CEN1241 varied according to colony age and was shown to be related to the ability of the biocontrol agent to suppress S. sclerotiorum. T. azevedoi CEN1241 VOCs reduced the size of S. sclerotiorum lesions on bean fragments in vitro and reduced disease severity in a greenhouse. This study demonstrated in a more applied way that the mechanism of antibiosis through the production of volatile compounds exerted by Trichoderma can complement other mechanisms, such as parasitism and competition, thus contributing to a better efficiency in the control of white mold in bean plants.

• 한국자원식물학회지
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• 제32권4호
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• pp.255-263
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• 2019

본 연구에서는 다양한 약리학적 활성을 가지는 것으로 알려진 감초 열수추출물(GRW)의 항암효능을 알아보기 위하여 인체 방광암 T24 세포에서 생존율 및 증식억제에 미치는 영향과 이와 연관된 apoptosis 유발 여부 및 관련 인자들의 발현 변화를 조사하였다. 먼저 GRW의 처리에 따른 증식억제 정도를 조사한 결과, GRW 처리 농도 의존적으로 생존율 및 증식억제 현상이 나타났으며, 핵의 형태 변화, DNA 단편화 및 apoptosis 유발에 관하여 조사한 결과 역시 GRW 처리 농도 의존적으로 증가됨을 확인할 수 있었다. 이는 GRW의 처리에 의한 암세포의 증식억제 및 형태적 변형이 암세포의 apoptosis 유발과 밀접한 관련이 있음을 시사하여 주는 것으로 사료된다. GRW 처리에 의한 apoptosis 유발에 관여하는 유전자의 탐색을 위하여 apoptosis와 연관성을 가지는 Bcl-2 family에 속하는 유전자의 발현을 조사한 결과 GRW 처리 농도 의존적으로 Bax 단백질의 발현증가와 더불어 Bcl-2 및 Bcl-xL 단백질의 발현감소가 관찰되었다(Fig. 3A). 이는 GRW에 의한 T24 세포의 apoptosis 유발에 Bcl-2 family에 속하는 유전자의 발현 조절이 중요한 역할을 하는 것으로 사료된다. 또한 GRW의 처리에 따른 MMP의 소실은 미트콘드리아 막의 교란이 유발되었음을 의미하는 것으로, 이러한 MMP 값의 변동은 Bcl-2 family 단백질의 발현 변화에 의한 것이라 추정된다. 한편 Apoptosis에 중요한 역할을 하는 것으로 알려진 caspase(-3/-8/-9)의 발현과 이들의 활성을 억제하는 IAP family (XIAP, cIAP-1, cIAP-2)의 발현에 GRW이 어떠한 영향을 미치는지를 조사한 결과, caspase-3, -8 및 -9의 활성형 단백질 발현 및 정량적 활성증가를 확인하였으며, IAP family 속한 3가지 단백질 모두 발현이 감소하는 것이 관찰되었다. 이상의 결과에서 GRW은 외인적 및 내인적 경로의 개시에 핵심적인 역할을 하는 caspase-8 및 -9의 활성을 모두 증가시켰으며, 이에 따른 caspase-3의 활성증가에 의하여 apoptosis가 유발되었음을 알 수 있었다. 이러한 두 경로의 동시 활성화에는 미트콘드리아의 기능 소실과 Bcl-2 및 IAP family의 발현 변화가 관여하고 있었으며, 특히 Bid의 발현 감소는 GRW에 의한 내인적 경로를 증폭시키는 효과로 작용했을 것이라 추정된다. 방광암의 치료에 보다 효과적인 생리활성을 갖는 물질을 발굴하고 그와 관련된 분자 및 세포수준에서의 기전을 밝히는 것이 중요하기에 본 연구의 결과는 향후 GRW로 수행될 추가 실험을 위한 기초자료로서 그 가치가 매우 높을 것으로 사료된다.

• BMB Reports
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• 제42권9호
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• pp.561-567
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• 2009

Although many plant-derived phenolic compounds display antioxidant effects in biological systems, their mechanism of action remains controversial. In this study, the mechanism by which p-coumaric acid (p-CA) performs its antioxidant action was investigated in bovine aortic endothelial cells under oxidative stress due to high levels of glucose (HG) and arachidonic acid (AA), a free fatty acid. p-CA prevented lipid peroxidation and cell death due to HG+AA without affecting the production of reactive oxygen species. The antioxidant effect of p-CA was not decreased by buthionine-(S,R)-sulfoximine, an inhibitor of cellular GSH synthesis. In contrast, pretreatment with p-CA caused the induction of peroxidases that decomposed t-butyl hydroperoxide in a p-CA-dependent manner. Furthermore, the antioxidant effect of p-CA was significantly mitigated by methimazole, which was shown to inhibit the catalytic activity of 'p-CA peroxidases' in vitro. Therefore, it is suggested that the induction of these previously unidentified 'p-CA peroxidases' is responsible for the antioxidant effect of p-CA.

• 대한임상검사과학회지
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• 제47권3호
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• pp.112-116
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• 2015

산화질소(Nitric Oxide, NO)는 생리학적, 병리학적으로 주요한 역할을 하고 있는 것으로 알려져 있다. 본 실험에서는 NO donor인 sodium nitroprusside (SNP)가 HaCaT 세포에서 apoptosis를 유도한다는 것을 DAPI염색과 PARP, caspase-3 단백질 절단을 western blot으로 확인하였다. SNP는 ER stress와 관련 있는 Bip, CHOP 유전자 발현에는 영향이 없었다. 최근 NAD+ 의존 deacetylase인 sirt1이 세포의 생존 및 사멸에 매우 중요한 단백질이라는 보고가 있다. 본 실험에서 SNP는 HaCaT 세포의 sirt1 유전자 발현을 감소시켰으며 이는 apoptosis와 관련이 있을 수 있다.

• STI Policy Review
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• 제4권2호
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• pp.96-112
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• 2013

The need for designing and implementing integrated policy was further emphasized in tandem with the increase in interest concerning policy coordination and interactions. An active discussion is taking place in the field of innovation policy concerning "integrated innovation policy," which considers innovation along with financial, regional development, social, and environmental policies together in a holistic manner. In Korea since the beginning of the 2000s, there were many attempts at implementing integrated innovation policy through the restructuring of the overall S&T administration system. For the purposes of taking an integrated approach to S&T policies as well as to S&T-related human resources, industrial, and regional development policies, the Roh Administration (February 2003~February 2008) elevated the S&T Minister to the level of Deputy Prime Minister as well as launching the Office of Science, Technology, and Innovation (OSTI) (October 2004 ~ February 2008) under the Ministry of Science and Technology. This study investigates the policy coordination activities of the OSTI from the perspective of policy integration. It deals with the background of the OSTI, its roles and responsibilities, the coordination process, and its achievements and limitations while discussing the important implications for developing effective policy measures with the hope of contributing to the development of theories of integrated innovation policy.

• 한국융합학회논문지
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• 제7권4호
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• pp.33-38
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• 2016

DDoS 공격은 네트워크나 서버 대역이 감당할 수 없는 수많은 양의 트래픽을 일으켜 서버를 마비시키는 공격수단으로 최근까지 지속적으로 이용되고 있다. 대부분 DDoS의 가장 큰 원인은 NTP라고 생각하지만, 최근 일어난 DDoS 공격들을 본다면 증폭기법을 이용한 SSDP 공격을 많이 사용하였음을 알 수 있다. SSDP의 특성상 소스 IP주소 위조와 증폭 요소를 가능하게 해주는 연결이 없는 상태 때문에 공격에 많이 활용되고 특히 웹캠, 공유기, 미디어, 스마트TV, 프린터 등 스마트 홈을 구성하는 IoT기기들에서 DDoS공격을 유발하는 도구로 주로 사용되는 프로토콜이기 때문에 점차 공격을 위한 서버들이 증가될 것으로 예상한다. 이는 단순한 IoT기기의 위협만이 아닌 사람의 생명이나 주요 정부기관 및 기업 시스템의 중요정보에 큰 위험을 가져올 수 있다. 본 논문에서는 IoT기기에서 발생하는 SSDP 프로토콜의 취약점을 이용한 DDoS공격기법을 알아보고 공격시나리오 및 대응 방법을 제안한다.

• 한국소프트웨어감정평가학회 논문지
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• 제15권2호
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• pp.129-140
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• 2019

In the IoT environment, there is a huge amount of heterogeneous devices with limited capacity. Existing trust evaluation methods are not adequate to accommodate this requirement due to the limited storage space and computational resources. In addition, since IoT devices are mainly human operated devices, the trust evaluation should reflect the social relations among device owners. There is also a need for a mechanism that reflects the tendency of the trustor and environmental factors. In this paper, we propose an adaptable trust evaluation method for SOA-based IoT system to deal with these issues. The proposed model is designed to minimize the confidence bias and to dynamically respond to environmental changes by combining direct evaluation and indirect evaluation. It is expected that it will be possible to secure trust through quantitative evaluation by providing feedback based on social relationships.

• 약학회지
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• 제54권2호
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• pp.112-121
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• 2010

The cytotoxic activity of 33,4,5-trihydroxybenzoic acid methylester and related compounds on the growth of normal cell lines, human skin melanoma cells and human oral epithelioid cell line were evaluated by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and 2,3-bis-[2-methoxy-4-nitro-5-sulfo-phenyl]-2-H-tetrazolium-5-caboxanilide (XTT) methods. 3,4,5-Trihydroxybenzoic acid methylester decreased the cell viability of human skin melanoma cells and human oral epithelioid cells shown by the MTT method and the cell adhesion activity of human skin melanoma cells and human oral epithelioid cells shown by the XTT method. In light microscopy, 100 ${\mu}M$ 3,4,5-trihydroxybenzoic acid methylester showed the highest cytotoxic activity. These results suggest that 3,4,5-trihydroxybenzoic acid methylester has a potential anticancer activity.

• 보건교육건강증진학회지
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• 제20권2호
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• pp.197-216
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• 2003

This study has been done in order to examine elementary school pupil's knowledge and attitudes on sex. The subjects of the study were 1904 elementary school pupils in the 5th and 6th grade of three elementary schools in Kyonggi-do, Korea. A guide book for the teachers on sexual education published by the Ministry of Education and Human Resources has been used as the tool for the study. Data analysis has been performed by using SPSS Win(l0.0) with mean, standard deviation, t-test, F-test(ANOVA), and Pearson's Correlation. The results show that the human relationship area showed the highest, next were physical development, sexual culture and sexual ethics, and the last were sex health and sexual psychology. The Pearson's correlation between each area of knowledge and attitude on sex show a significant correlation statistically in the level of p＜.001 in all areas. When examined individually the highest correlation was demonstrated between physical development and sexual health and human relationship by r＝.573, while it demonstrated only r＝.265 the lowest between sexual ethics and physical development.