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Reduction of long-term potentiation at Schaffer collateral-CA1 synapses in the rat hippocampus at the acute stage of vestibular compensation

  • Lee, Gyoung Wan;Kim, Jae Hyo;Kim, Min Sun
    • The Korean Journal of Physiology and Pharmacology
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    • v.21 no.4
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    • pp.423-428
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    • 2017
  • Vestibular compensation is a recovery process from vestibular symptoms over time after unilateral loss of peripheral vestibular end organs. The aim of the present study was to observe time-dependent changes in long-term potentiation (LTP) at Schaffer collateral-CA1 synapses in the CA1 area of the hippocampus during vestibular compensation. The input-output (I/O) relationships of fEPSP amplitudes and LTP induced by theta burst stimulation to Schaffer's collateral commissural fibers were evaluated from the CA1 area of hippocampal slices at 1 day, 1 week, and 1 month after unilateral labyrinthectomy (UL). The I/O relationships of fEPSPs in the CA1 area was significantly reduced within 1 week post-op and then showed a non-significant reduction at 1 month after UL. Compared with sham-operated animals, there was a significant reduction of LTP induction in the hippocampus at 1 day and 1 week after UL. However, LTP induction levels in the CA1 area of the hippocampus also returned to those of sham-operated animals 1 month following UL. These data suggest that unilateral injury of the peripheral vestibular end organs results in a transient deficit in synaptic plasticity in the CA1 hippocampal area at acute stages of vestibular compensation.

Development of a New Synthetic Medium Composition for the Submerged Culture of Phellinus linteus (Phellinus linteus의 액체배양을 위한 새로운 합성배지의 개발)

    • KSBB Journal
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    • v.14 no.2
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    • pp.167-173
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    • 1999
  • A new synthetic medium was developed for the submerged mycelial cultures of Phellinus linteus. The medium for maximum mycelial growth of Phellinus linteus (3 days incubation, 28$^{\circ}C$, pH 5) consisted of (per 1 L): glucose, 90 g peptone, 10 g soluble starch, 10 g yeast extract, 3 g KH2PO4, 1 g MgSO4.7H2O, 1 g and CaCl2, 0.1 g. The concentrations of glucose, peptone, yeast extract, KH2PO4, MgSO4.7H2O, and CaCl2 were examined in the ranges of 10~90 g/L, 0~10 g/L, 0~15 g/L, 0~2 g/L, 0~1 g/L, and 0~0.5 g/L, respectively. The dry weight of mycelium in 3 days increased to 16.79 mg/mL using the new synthetic medium. The optimum temperature for mycelial growth of Phellinus linteus was 28$^{\circ}C$. The concentrations of KH2OP4, CaCl2, and yeast extract, which gave the maximum mycelial growth of Phellinus linteus, existed in the concentration ranges examined in this study. But, in the cases of other compositions (MgSO4.7H2O, peptone, and glucose), the mycelial growth of Phellinus linteus increased with the concentration in the ranges.

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Cloning and Site-Directed Mutagenesis of Musca domestica Acetylcholinesterase for Enhancing Sensitivity to Organophosphorus and Carbamate Insecticides

  • Kim, Chung-Sei;Kim, Su-Il
    • Journal of Microbiology and Biotechnology
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    • v.16 no.11
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    • pp.1760-1772
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    • 2006
  • Mature acetylcholinesterase (AChE) gene (gm, 1,836 bp) was cloned from the housefly and successfully expressed in the E. coli CodonPlus (DE3) RIL system (GM-E, 72 kDa) with a yield of 1,630 mU/g fresh cells. Using the gm, 10 kinds of mutants were constructed and expressed for enhancing sensitivity to insecticides. The sensitivity of these mutants to five kinds of organophosphate (OP) and three carbamate insecticides was investigated by measuring the apparent bimolecular inhibition constant ($k_i=k_2/K_d$). Surprisingly, the sensitivity of quadruple mutant IGFT was enhanced as much as 7-fold for acephate, 164-fold for demeton-S-methyl, 484-fold for dichlorvos, 523-fold for edifenphos, 30-fold for ethoprophos, 30-fold for benfuracarb, 404-fold for carbaryl, and 107-fold for furathiocarb, compared with that of GM-E, although the sensitivity of each single point mutant was slightly increased. These mutational studies indicated that (i) contradictory to Walsh et al. [39], the residue 327 is the important key residue for enhancing sensitivity as much as the residue 262, (ii) the residue 82 and additional residues of 234, 236, and 585 are also important, and (iii) sensitivity was cooperatively accelerated as the number of strategic mutations increased.

What Kind of Job Characteristics are Important for Improving Students' Competencies in Co-op Education : KoreaTech IPP Case

  • Lee, Ji-young;Lee, Sang-kon;Lee, Ji-yeon
    • Journal of Practical Engineering Education
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    • v.11 no.2
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    • pp.219-229
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    • 2019
  • The purpose of this study was to examine the effect of on-site training environments and goals on learning outcomes of trainees. For this purpose, the study analyzed survey data collected from 752 students who participated in KOREATECH's Industry Professional Practice (IPP) on-site training program from 2017 to 2018. The following summarizes some important findings from the study results: First, on examining whether the effect of job characteristics on the academic competencies differs according to the characteristics of the group, it was found that the factors that affect the whole group were job content, coaching, and welfare benefits, but the effect of job scope was statistically significant. Second, on examining whether the effect of job characteristics on job competencies differs according to the characteristics of the group, it was found that the factors that affect the whole group were job content, coaching, and job scope, but the effect of welfare was statistically significant. Analyzing by training goals, it was found that the major influencers were job content in the Recruitment-intended group; and job content, coaching and job scope in the Training-intended group. Based on the results, the implications of the study and follow-up studies are suggested.

Intelligent AGC Circuit Design (지능형 AGC 회로 설계)

  • Zhang Liang;Kim Jong-Won;Seo Jae-Yong;Cho Hyun-Chan;Jeong Goo-Chul
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 2006.05a
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    • pp.302-305
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    • 2006
  • A problem that arises in most communication receivers concerns the wide variation in power level of the signals received at the antenna. These variations cause serious problems which can usually be solved in receiver design by using Automatic Gain Control (AGC). AGC is achieved by using an amplifier whose gain can be controlled by external current or voltage. However, the AGC circuit does not respond to rapid changes in the amplitude of input. If input changes instantaneously, then even if op-amps could follow the change, the envelope detector capacitor could not, since the capacitor's voltage could not change instantaneously. To alleviate this deficiency, we present Improved Automatic Gain Control Circuit (IAGCC) replacing AGC circuit to FLC.

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A Study on an Utilization Method of Marine Information - on the basic of the Area, Tong-Yeong - (수산정보 활용방안에 관한 연구 - 통영지역을 중심으로-)

  • 박연식;이상욱;김영수;정근회;임재홍
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.1 no.1
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    • pp.3-12
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    • 1997
  • In this study, in Tongyeong area the distribution of marine products that were produced and treated through fishing and cultivating industry and the trend of main fisheries were investigated for fisheries distribution system and fisheries official circles, and then a structural model of fisheries distribution system for direct transaction was suggested under internet environment, which is an important scheme offering a necessary information rapidly and practically to both producers and consumers. Database system was structured according to distributed process. An op-elative scheme were suggested for sewers of local area information center, complementing functions to exceed each server's ability.

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Standardized Progress Measurement Package (건설진도율 산정을 위한 진도관리단위에 관한 연구)

  • Jung Youngsoo;Kang Seunghee;Chin Sangyoon;Kim Yeasang;Chung Moonhun;Park Soonchan
    • Proceedings of the Korean Institute Of Construction Engineering and Management
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    • 2004.11a
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    • pp.565-570
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    • 2004
  • The construction Progress is widely used 3s a critical index for effective project management. However, the methods, structure, data, and accuracy of progress measurement may vary depending on specific characteristics of the project, organization, or location. Even in an organization, different projects may utilize different measurement methods to effectively achieve their own management purpose. The excessive effort required to manipulate very detailed progress data is also an issue Therefore the purpose of this study is to dove]op an automated progress measurement model utilizing standard progress measurement package (SPMP).

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Determination factors of Purchase Expansion for Environment friendly Agricultural Products on Consumers (소비자의 친환경농산물 구매확대 결정요인 분석)

  • Ro Chae Yeong;Ahn Pyong Ryol
    • Journal of Family Resource Management and Policy Review
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    • v.9 no.1
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    • pp.1-15
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    • 2005
  • This study was designrd to determination factors of purchase expansion for environment-friendly agricultural products on consumers. The 310 subjects formatted questionnaires were analyzed to frequency, percentage, descriptives, T-test and logistic regression by SPSS WIN 10.0. The results were as follows : First, it is proved that degree of cognition, standard of information acquisition, basis of valuation were significant variables, Second, it is revealed that the producer and the direct deal, the consumer organization's co-op and the food floor of large-sized discount hoarded goods for purchase places of environment-friendly agricultural products were significant variables. Third, it is proved that reduction in a price the improvement matter for purchase expansion of environment-friendly agricultural products was determination factors. Forth, it is proved that age and income for purchase expansion of environment-friendly agricultural products was determination factors. Accordingly, we should find the positive factors of purchase and consumption that it will become an important subject for environment-friendly agricultural products on consumers.

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ARPES study of Ultrathin Fe Grown on Cu (001) surface

  • Poornima, L.;Oh, Y.R.;Park, Y.S.;Kim, W.;Kim, C.G.;Hong, J.;Hwang, Chan-Yong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.350-350
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    • 2011
  • The spin structure of Fe over layers on Cu (001), especially in region II is one of the unsolved problem for many years. We study the out - of - plane (OP) Fermi surfaces (FSs) of 7 monolayer Fe/Cu (001) films using angle resolved photo emission spectroscopy (ARPES). Ultrathin Fe was grown on Cu (001) substrate at room temperature and the experimental measurements were carried out at room temperature and low temperature. Fermi surfaces measured about $\frac{1}{4}$ of the Brillouin Zone (BZ) using photon energies ranging from 170 eV to 280 eV. Our results confirmed that ferromagnetic signal at 7 ML Fe on Cu (001) is nearly zero. These results are consistent with our recent x-ray magnetic circular dichroism (XMCD) and surface magneto - optic Kerr effect (SMOKE) experiments. Based on our observations we have made a simple model of this system, which explains all the experimental results.

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Unambiguous Identification of Fugitive Pollutants and Determination of Annual Emission Flux as Diurnal Monitoring Mode

  • Chang, Shih-Yi;Tso, Tai-Ly;Lo, Jiunn-Gung;Huang, Jer-Luen;Lin, Cheng Ming
    • Analytical Science and Technology
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    • v.8 no.4
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    • pp.731-738
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    • 1995
  • Toxic air pollutants are investigated in a petrochemical industrial park in Taiwan by using a movable open path FTIR. The results show the qualitative and quantitative analysis of emission gases from plants, and also provide the emission rate of different compounds. More than twenty compounds under usual operation are found from this industrial park. The concentration variation with time can be correlated exactly with wind direction. It means that by changing the measuring points, the source of emission can be unambiguously identified. An EPA proved PAL model is applied to estimate the emission rate of either a point or an area source. Local atmospheric stability is determined by releasing the $SF_6$ tracer. The origins of errors come mainly from the uncertainty of source's configuration and the variation of meteorological condition. Through the continuous measurement (half an hour base in this study) of OP-FTIR sensor, the maximum value of emission rate and the annual amount of emission can be derived. The emission rate of the measured toxic gases are derived by the model technique and the results show that the emission amount are in the order of ten to hundred tons per year.

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