• 한국식품영양학회지
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• 제17권3호
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• pp.278-285
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• 2004

본 논문은 rye bread 제조에 있어서, water, rye flour 그리고 vital gluten이 rye bread의 관능 특성에 미치는 영향에 대하여 response surface methodology, 관능검사 및 통계적 분석을 통하여 연구하였다. Response surface model은 rye bread의 최적 variation을 결정하고 관찰되어진 결과를 평가하기 위하여 사용되었으며, 본 실험은 water 57, 62, 67%, rye flour 0, 10, 30, 50% 그리고 vital gluten 0, 1, 3, 5%의 12가지 조합을 하여 실험하였다. 실험 결과 water 67%, rye flour 10% 그리고 vital gluten 3%일 때 높은 specific volume의 rye bread가 생산되었으며, water 62%, rye flour 10%그리고 vital 히uten 5%일 때 전체적인 관능치가 높은 rye bread가 생산되었다. 또한 specific volume과 전체적인 기호도도 높은 것으로 나타났으며, 이러한 결과는 bread loaf volume과 소비자의 기호도가 직접적인 관계가 있음을 보여준다. 본 논문은 rye bread의 생산에서 다양한 water, rye flour그리고 vital gluten의 배합에 대한 정보를 제시하였으며, 표준화 최적화 방법에 대한 기초 정보를 제공하는데 큰 의의를 두었다. 또한 이 결과는 water, rye flour 그리고 vital gluten이 rye bread의 생산에서 다양한 방법으로 배합될 수 있으며, 제빵의 기능적 특성을 최적화 하는데 기여할 것으로 기대된다.

• 한국식품조리과학회지
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• 제31권3호
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• pp.264-279
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• 2015

The principal objective of this study was to evaluate the effects of the addition of sourdough to various grain flours in bread, specifically, in regards to the physicochemical characteristics of bread dough, sensory evaluation, and bread storage. As the incubation time of sourdough increased, the total titratable acidity increased. Viable yeast counts of sourdough increased consistently until the third day, while lactic acid bacteria counts increased until the second day. The weight of breads containing sourdough made with rye flour, strong flour, and Korean wheat flour were higher than that of the control. However, the height, volume, and specific volume of control were higher than those of the groups with sourdough made with various grain flours. The pH of breads containing sourdough was lower than that of the control, while the total titratable acidity and moisture content were higher than those of the control. In analyzing the visible mold colony during the five days of storage at $30^{\circ}C$, mold growth in breads containing sourdough made of Korean wheat flour, barely flour, and rye flour was retarded. In the color measurement, the L values of the control and bread containing sourdough made with barley flour were higher than that of the other groups after five days. The a value of bread containing sourdough made of rye flour was higher, and the b values of breads containing sourdough made of Korean wheat flour, barley flour and rye flour were higher than those of the other groups after five days. The hardness of breads containing sourdough increased as storage time increased, where as breads containing sourdough made of Korean wheat flour, Korean whole wheat flour, and rye flour revealed no significant differences with control group. Sensory evaluation scores in terms of after swallowing, taste, and overall preference of bread containing sourdough made of Korean wheat flour was higher than those of the control group.

• Journal of Biosystems Engineering
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• 제35권6호
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• pp.408-412
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• 2010

Rheological properties of cereals such as rye are great important for the design of die for extrusion and the development of models for extrusion process. Therefore, this study was carried out to analyze the rheological properties according to moisture content of rye flour and extrusion temperature. Rheological properties of rye flour were investigated by using a capillary rheometer for moisture content of three levels (30, 35, 40%) and extrusion temperature of three levels (120, 130, $140^{\circ}C$). Determination coefficients for the relationship between apparent shear stress ($\tau_{ap}$) and apparent shear velocity ($\gamma_{ap}$) were relatively high in the range of 0.973 ~ 0.997 under each extrusion condition. The values of consistency index (K) was decreased with increasing moisture content and extrusion temperature. However, the value of flow behavior index (n) presented the highest value at moisture content of 35%, but it was not affected by extrusion temperature.

• Food Science and Biotechnology
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• 제18권2호
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• pp.456-462
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• 2009

In comparison to processed wheat flour products, there is no established method for determining the water absorption of rye flour. The aim of this study was to work out a method using the Planetary mixer P 600 for the determination of water absorption (WA) and the rheological properties in rye dough made from rye flours of 4 different types (I-IV). In the correlation analysis showed that WA had positive correlation with ash, beginning of gelatinization, $125-160{\mu}m$ particles, sedimentation values at 20 and 25 min, bread yield, pH, and total titratable acidity, but had negative correlation with initial viscosity, gelatinization maximum, viscosities on swelling at 25, 28, and $31^{\circ}C$. The WA determined by the Planetary mixer P 600 agreed well with the experimental baking tests.

• 동아시아식생활학회지
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• 제18권1호
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• pp.87-94
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• 2008

In this study, a nanatural fermentation starter formulation was developed for manufacturing bread products by substituting baker's yeast with naturally fermented raisin extract and sourdough. Four experimental groups containing 2.5, 5.0, 7.5, 10% naturally fermented raisin extract per 2,000 g of flour were compared based on quality characteristics, including the fermentation power on dough expansion, specific volume, baking loss, water activity, color, textural characteristics, and internal surface appearance. The activities of the naturally fermented raisin extract were examined in terms of pH changes, total titratable acidity, brix, and viable yeast counts. The raisin extract, which was cultured for 7 days at 30$^{\circ}C$, smelled of alcohol and produced $CO_2$. Yeast were also found in the extract after separation. As the incubation time of the raisin extract and sourdough increased, pH decreased, while total titratable acidity increased. The brix of the raisin extract increased until the $2^{nd}$ day of fermentation, and viable yeast counts increased until the $5^{th}$ day however, these gradually decreased by the $7^{th}$ day. The fermenting power on dough expansion increased in the bread with increasing incubation time. The bread samples containing 7.5% and 10% raisin extract had significantly higher specific volumes than the other samples. Baking loss was minimal with the 2.5% extract substitution. In analyzing the crumb, water activity, redness, and yellowness were highest in the 10.0% raisin extract bread samples, and lightness was maximal in the 5.0% group. In terms of textural characteristics, hardness was lowest with the 2.5% extract substitution. Gumminess, springiness, and chewiness were not significantly different among the bread samples. Cohesiveness was highest at the 7.5% extract substitution level, and resilience was lowest at the 10% level. In conclusion, based on the results, a natural fermentation starter formulated with 2.5% naturally fermented raisin extract (1 part raisins and 1.5 parts water) and 70% sourdough (1 part rye flour and 1 part water) has high potential as a baker's yeast substitute for making naturally fermented bread.

• 한국식품영양과학회지
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• 제37권5호
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• pp.625-632
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• 2008

본 연구에서는 sourdough의 대체량을 20%, 40%, 60% 및 80%로 달리하여 제조한 호밀-밀 혼합빵의 품질 특성을 실험하였다. Sourdough의 pH와 총산도는 배양시간이 길어질수록 pH는 감소하는 경향을 나타내었고, 총산도는 증가하였다. 호밀-밀 혼합반죽의 sourdough 대체량이 증가할수록 pH는 감소하는 경향을 나타내었고, 총산도는 감소하는 경향을 나타내었다. 호밀-밀 혼합빵의 pH는 대조군보다 sourdough 대체군들이 유의적으로 낮게 나타났고, 총산도는 높게 나타났다. 발효팽창력은 발효시간이 길어질수록 유의적으로 증가하는 경향을 나타내었으며, sourdough 20% 대체군이 111.43%로 가장 높게 나타났다. 비용적은 sourdough 20% 대체군이 1.87 mL/g로 가장 높았다. 굽기 손실률은 sourdough 80% 대체군이 7.39%로 가장 낮았다. 수분활성도는 대조군이 0.919로 가장 높았고, 대조군보다 sourdough 대체군들이 유의적으로 낮게 나타났다. 명도, 적색도 및 황색도는 sourdough 대체군들이 대조군보다 유의적으로 높게 나타났다. 견고성, 점착성 및 씹힘성은 대조군이 가장 낮았고, 대조군보다 sourdough 대체군들이 유의적으로 높게 나타났다. 한편, 부착성은 대조군이 가장 높았고, sourdough 대체군들이 대조군보다 유의적으로 낮게 나타났다. 부서짐성, 응집성 및 복원성은 대조군과 sourdough 대체군들 간에는 유의적인 차이가 나타나지 않았다. 탄력성은 sourdough 20% 대체군이 0.746으로 가장 높았고, 40% 대체군이 0.629로 가장 낮았다. Sourdough 20% 대체군을 제외하고, 겉껍질과 로프의 양 옆면 사이에 브레이크(break)와 슈레드(shred)가 관찰되었다. 관능검사 결과에서 빵 속살의 색상, 기공의 균일성, 빵 속살의 경도, 입안에서의 점착성 및 전체적인 기호도는 sourdough 대체량이 증가할수록 유의적으로 감소하는 경향을 보였고, 호밀향과 쓴맛은 이와 반대의 경향을 보였다. 기공의 밀도, 탄력성 및 신맛은 대조군보다 sourdough 대체군들의 점수가 유의적으로 높게 나타났다. 이상의 결과를 종합해 보면, 대조군의 호밀-밀 혼합빵이 관능검사에서 가장 높은 점수를 나타냈고, sourdough 대체량이 많아질수록 관능검사 점수가 다소 떨어지기는 하였지만, 전체적인 품질특성을 고려해 보면 $20{\sim}60%$ 대체는 sourdough의 향과 맛을 지니면서도 상당히 우수한 빵제품을 생산해 낼 수 있을 것으로 사료된다.

• 한국식품과학회지
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• 제46권3호
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• pp.269-275
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• 2014

메밀로부터 분리한 메밀단백질(BWP)을 토끼에게 면역하여 생산한 특이항체를 이용하여 가공식품 중 메밀의 검출을 위한 간접경합 효소면역측정법(ciELISA)을 확립하였다. 이때, BWP의 검출범위는 $0.05-100{\mu}g/mL$이었다. 특이항체 교차반응 결과, BWP, 메밀가루, 통메밀에 대한 반응성은 각각 100, 17.9, 11.8%를 나타내었으며 그 외 곡물 14종에 대한 반응성은 거의 나타나지 않아 메밀에 대한 특이성이 매우 높았다. 메밀가루는 $60-90^{\circ}C$에서 반응성이 평균 83.0%를 나타내었으며 $100^{\circ}C$에서는 44.5%로 반응성이 감소하였다. 메밀가루에 대한 spike test에서 메밀 생면, 삶은 면, 묵, 메밀곡물가루에서 평균 분석회수율이 각각 99.1, 98.6, 81.1, 104%로 매우 높게 나타났다. ciELISA에 의하여 22점의 시판시료 중 메밀의 함유량을 조사한 결과, 메밀 함유 표시된 12점에서 평균 분석회수율은 31.6%로 나타났으나 시료 중 메밀의 정성적 검출은 100%로 매우 우수하였다. 본 연구에서 개발한 ciELISA는 가공식품에 함유된 메밀을 검출하는데 매우 효과적으로 활용할 수 있을 것이다.

• 한국식품영양학회지
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• 제21권4호
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• pp.492-498
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• 2008

This study investigated the quality characteristics of the white pan bread prepared with pomegranate powder. Three different powder concentration levels of 1%, 2% and 3% were added to flour to make the bread. The contents of moisture and crude fat contents of the bread added with to which pomegranate powder was added were lower than that of the control group. However, the ash content was not did not significantly different differ significantly among groups. The pH increased with increasing concentrations of pomegranate powder concentration. The weight of bread increased with increasing concentrations of pomegranate powder concentration, while whereas the volume and baking loss rate of bread concomitantly decreased. In terms of color values, with increase increases in of the concentration of pomegranate powder concentration, induced a reduction in the L value decreased, but and a concomitant increase in the a and b values increased. The water activity in the bread decreased by with increasing pomegranate powder concentration, and was the highest in the control group. In the Upon texture analyzer measurement analysis, the hardness of the bread was shown to increased increase with increases of in the concentration of pomegranate powder concentration, but the springiness decreased. In the With regard to the results of sensory evaluation, the quality of the 1% pomegranate powder bread showed the evidenced the highest in taste, flavor, and overall acceptability. The color, appearance, mouth feel, and texture of the bread decreased with the increasing pomegranate powder content concentrations. From According to the results of this study, the white bread prepared with 1% pomegranate powder content was shown judged to have the best highest quality.

• Asian-Australasian Journal of Animal Sciences
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• 제28권6호
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• pp.788-795
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• 2015

Two dimensional-fluorescence difference gel electrophoresis (2D DIGE) is an emerging technique for comparative proteomics, which improves the reproducibility and reliability of differential protein expression analysis between samples. The purpose of this study was to investigate bovine pregnancy-specific proteins in the proteome between bovine pregnant and non-pregnant serum using DIGE technique. Serums of 2 pregnant Holstein dairy cattle at day 21 after artificial insemination and those of 2 non-pregnant were used in this study. The pre-electrophoretic labeling of pregnant and non-pregnant serum proteins were mixed with Cy3 and Cy5 fluorescent dyes, respectively, and an internal standard was labeled with Cy2. Labeled proteins with Cy2, Cy3, and Cy5 were separated together in a single gel, and then were detected by fluorescence image analyzer. The 2D DIGE method using fluorescence CyDye DIGE flour had higher sensitivity than conventional 2D gel electrophoresis, and showed reproducible results. Approximately 1,500 protein spots were detected by 2D DIGE. Several proteins showed a more than 1.5-fold up and down regulation between non-pregnant and pregnant serum proteins. The differentially expressed proteins were identified by MALDI-TOF mass spectrometer. A total 16 protein spots were detected to regulate differentially in the pregnant serum, among which 7 spots were up-regulated proteins such as conglutinin precursor, modified bovine fibrinogen and IgG1, and 6 spots were down-regulated proteins such as hemoglobin, complement component 3, bovine fibrinogen and IgG2a three spots were not identified. The identified proteins demonstrate that early pregnant bovine serum may have several pregnancy-specific proteins, and these could be a valuable information for the development of pregnancy-diagnostic markers in early pregnancy bovine serum.

• 한국식품영양과학회지
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• 제36권6호
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• pp.785-793
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• 2007

본 연구에서는 홍국의 첨가량을 달리하여 sourdough starter를 전통적 방법으로 제조하고, 발효시간에 따른 sourdough starter의 이화학적 특성을 분석하고, sourdough bread의 texture 특성 및 관능검사를 실시하여 이의 품질특성을 조사하였다. 홍국, 강력분, 호밀분의 수분 함량은 6.15, 12.53, 8.56%로 나타났으며, 회분 함량은 0.15, 0.44, 1.64%로 나타났다. 조지방 함량은 0.97, 1.16, 2.49%로 나타났으며, 조단백질 함량은 7.30, 12.57, 11.18%로 나타났다. 탄수화물 함량은 85.42, 73.29, 76.14%로 나타났다. Sourdough starter의 pH는 모든 시료가 3일까지 저하되었으며, 홍국의 첨가량이 증가할수록 pH는 저하되는 경향을 나타내었다. 환원당과 총당의 경우 SD1은 3일, $SD2{\sim}4$는 1일까지 유의적으로 증가한 후 감소하였으며, 홍국의 첨가량이 증가할수록 높게 나타났다. 젖산균의 균수는 모든 시료들이 0일에서 나타나지 않았고, 2일에서 최대를 나타냈다. 효모의 균수는 SD1이 4일의 발효시간 동안 나타나지 않았으며, $SD2{\sim}4$는 3일까지 증가하였다. Mixograph 측정 결과 peak time, peak value 및 width of tail은 CSB가 가장 높게 나타났으며, $SDB1{\sim}3$은 홍국의 함유량이 증가할수록 낮게 나타났다. 발효 팽창력은 $SDB1{\sim}3$이 CSB보다 크게 나타났으며, 반죽 직후부터 45분간의 발효팽창력은 SDB1, SDB2가 SDB3보다 크게 나타났다. 비용적과 굽기 손실률의 측정 결과 SDB1이 가장 크게 나타났으며, 각각의 시료들은 비용적이 증가할 때 굽기 손실이 비례하여 증가하는 것으로 나타났다. 색도의 측정 결과 L값은 CSB가 가장 높은 값을 나타내었으며, 홍국의 함유량이 증가할수록 유의적으로 감소하였다. a값은 CSB가 가장 낮은 값을 나타내었으며, 홍국의 함유량이 증가할수록 유의적으로 증가하였다. b값은 CSB가 가장 낮은 값을 나타내었으며, 홍국의 함유량이 증가할수록 유의적으로 증가하였다. 물성측정 결과 경도와 응집성은 각 시료들 간의 유의적인 차이가 나타나지 않았다. 탄력성과 부서짐성은 CSB가 가장 낮았으며, 홍국의 함유량이 증가할수록 증가하였다. 점착성은 SDB1이 가장 낮았으며, 홍국의 함유량이 증가할수록 증가하였다. 관능검사 결과 기공의 균일성은 SDB1이 가장 균일한 것으로 나타났으며, 색은 홍국의 함유량이 증가할수록 높게 나타났다. 경도, 탄력성, 단맛 및 신맛 등은 홍국 함유량이 증가할수록 증가하는 것으로 나타났다. 이취는 SDB1이 가장 적게 나는 것으로 나타났으며, 전반적인 기호도는 SDB1이 가장 높았다. 따라서 홍국을 10% 첨가한 sourdough starter를 3일 동안 발효한 후 반죽에 첨가하여 sourdough bread를 제조할 때 품질이 가장 우수한 제품을 얻을 수 있었다.