• 농촌지도와개발
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• 제16권3호
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• pp.643-668
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• 2009

The purpose of this study is to estimate the value of the sociocultural multifunctionality of rural areas in Korea. The sociocultural multifunctionality of rural areas consists of emotional comfort, green landscape, cultural heritage, and rural viability values. The value of the sociocultural multifunctionality was assessed by contingent valuation method incoporating preference uncertainty. The log-logit models indicated that households were willing to pay 14,027~26,757 won per month and the model with preference uncertainty gave the highest WTP. WTP was affected by respondent's sex, location, awareness of relation with multifunctionality and others. The total value of sociocultural multifunctionality of rural areas in Korea estimated to 2,691~5,134 billion won per year.

• 농촌지도와개발
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• 제5권2호
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• pp.153-164
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• 1998

Rural Environmental Planning (REP) is a method used by citizens in small towns and rural areas to plan their own future. REP assumes that the primary social value of rural people is to enhance a community's longterm viability by respecting the carrying capacity of the natural environment. REP derives its strength from the direct participation of those people affected by a plan. They determine its goals, shape its content, and implement its components. And both the rural environmental planner and the citizens involved in planning in their community must have a working knowledge of the legal framework of planning. In the results, REP reduces the cost of planning by drawing public agencies, not-for-profit organizations, volunteers from the private sector, and all vital resources available to rural people when they plan for their future.

• Journal of Biosystems Engineering
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• 제38권4호
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• pp.312-317
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• 2013

Purpose: Conventional methods used to evaluate seeds viability are destructive, time consuming, and require the use of chemicals, which are not feasible to implement to process plant in seed industry. In this study, the effectiveness of Fourier transform near infrared (FT-NIR) spectroscopy to differentiate between viable and nonviable watermelon seeds was investigated. Methods: FT-NIR reflectance spectra of both viable and non-viable (aging) seeds were collected in the range of 4,000 - 10,000 $cm^{-1}$ (1,000 - 2,500 nm). To differentiate between viable and non-viable seeds, a multivariate classification model was developed with partial least square discrimination analysis (PLS-DA). Results: The calibration and validation set derived from the PLS-DA model classified viable and non-viable seeds with 100% accuracy. The beta coefficient of PLS-DA, which represented spectral difference between viable and non-viable seeds, showed that change in the chemical component of the seed membrane (such as lipids and proteins) might be responsible for the germination ability of the seeds. Conclusions: The results demonstrate the possibility of using FT-NIR spectroscopy to separate seeds based on viability, which could be used in the development of an online sorting technique.

• Reproductive and Developmental Biology
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• 제36권4호
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• pp.269-273
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• 2012

The present study was to assess the in vitro viability and sexing rate of bovine embryos. Blastocysts were harvested on day 7~9 day after insemination(in vitro and in vivo), and the sex of the embryos was examined using the LAMP method. Embryo cell biopsy was carried out in a $80{\mu}l$ drop $Ca^{2+}$, $Mg^{2+}$ free D-PBS and, biopsied embryos viability were evaluated after more 12 h culture in IVMD culture medium. The formation of recovered embryo to expanded and hatching stages had ensued in higher of sexed embryo in vivo than in vitro (100% vs. 89%, p<0.05), and in vitro, the rates of degeneration after sexing were significantly (p<0.05) higher in vitro than in vivo(11% vs. 0.0%). The rates of the predicted sex were female 61% vs. 56%, and male 39% vs. 44% in vivo and in vitro, respectively. The rates of survival following different biopsy methods were seen between punching and bisection method in vivo and in vitro (100% vs. 89% and 100% vs, 78% respectively). Biopsy method by punching was significantly (p<0.05) higher than bisection between produced embryos in vivo and in vitro. The present data indicate that with microblade after punching for embryo sexing results in high incidence of survivability on development after embryo biopsy. It is also suggested that LAMP-based embryo sexing suitable for field applications.

• 한국지역사회생활과학회지
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• 제15권4호
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• pp.113-125
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• 2004

The objective of this study was to analyze the evaluation of the rural resident's on their community at view of community life satisfaction. The specific purposes of this study were to identify the degrees of community life satisfaction, consciousness of rural community according to the individual variables. The data were gathered from 1,870 rural residents by the structured interview in the 187 communities sampled by multi-staged cluster stratified sampling method in November, 2003. The major findings of this study were as follows; first, the degree of the rural residents'community life satisfaction was generally low, especially the rural residents were dissatisfied with the opportunities of leisure and education environment in their communities, and by half were satisfied with housing environment. Second, as consciousness of rural community, the degree of rural resident's joining at community meeting was high, and openness to the outside residents' was high. third, the most important factor for consciousness of rural community were community organization, good neighbor. As result of this study, the following conclusion and recommend can be drawn. First, the level of rural resident's community life satisfaction is overall low. Especially it is needed policy efforts and priority investment for advancing satisfaction on leisure and education environment in rural community. Second, to foster rural resident's community consciousness, community orgaruzatlon Is first of all supported and activated. Through viability of these community organization, rural resident's participation in community welfare will be enhanced

• 농업과학연구
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• 제47권3호
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• pp.657-665
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• 2020

The objective of this study was to investigate whether supplementation of pentoxifylline (PTX; phosphodiesterase inhibitor) to thawed boar semen improves the post-thaw motility of sperm and affects the efficiency of artificial insemination (AI) and further development. To determine the concentration of PTX for AI, frozen-thawed semen was incubated with 0, 5, 10, and 20 mM PTX in an extender freezing medium, respectively, after thawing. Kinematic properties of sperm were examined with a computer-assisted semen analysis (CASA) system. In addition, viability and mitochondrial activity were also tested by LIVE/DEAD and a MitoTracker kit. There were no significant differences in the kinetic parameters of thawed sperm between control and treatment groups, but overall assessment parameters such as motility and rapid progressive were higher in the 10 mM PTX group. In the viability and mitochondrial assay, there were no significant differences observed in the PTX treatment, compared to the control. For further analysis, artificial inseminations were performed using frozen semen and 10 mM PTX treated cryopreserved semen, respectively. There were no differences in pregnancy rates and fetus weights among the groups until 30 and 40 days, but litter size was reduced and relatively low-birth weight was observed in the PTX group. In summary, our findings suggest that enhancement of in vitro sperm quality or non-toxicity supplemented by PTX may have detrimental effects on fetus development.

• Asian-Australasian Journal of Animal Sciences
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• 제32권2호
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• pp.257-264
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• 2019

Objective: Dairy cattle nutrient requirement systems acknowledge amino acid (AAs) requirements in aggregate as metabolizable protein (MP) and assume fixed efficiencies of MP used for milk protein. Regulation of mammary protein synthesis may be associated with AA input and milk protein output. The aim of this study was to evaluate the effect of nanoemulsified methionine and cysteine on the in-vitro expression of milk protein (casein) in bovine mammary epithelial cells (MAC-T cells). Methods: Methionine and cysteine were nonionized using Lipoid S 75 by high-speed homogenizer. The nanoemulsified AA particle size and polydispersity index were determined by dynamic light scattering correlation spectroscopy using a high-performance particle sizer instrument. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to determine the cytotoxicity effect of AAs with and without nanoionization at various concentrations (100 to $500{\mu}g/mL$) in mammary epithelial cells. MAC-T cells were subjected to 100% of free AA and nanoemulsified AA concentration in Dulbecco's modified Eagle medium/nutrient mixture F-12 (DMEM/F12) for the analysis of milk protein (casein) expression by the quantitative reverse transcription polymerase chain reaction method. Results: The AA-treated cells showed that cell viability tended to decrease (80%) in proportion to the concentration before nanogenesis, but cell viability increased as much as 90% after nanogenesis. The analysis of the expression of genetic markers related to milk protein indicated that; ${\alpha}_{s2}$-casein increased 2-fold, ${\kappa}$-casein increased 5-fold, and the amount of unchanged ${\beta}$-casein expression was nearly doubled in the nanoemulsified methionine-treated group when compared with the free-nanoemulsified methionine-supplemented group. On the contrary, the non-emulsified cysteine-administered group showed higher expression of genetic markers related to milk protein ${\alpha}_{s2}$-casein, ${\kappa}$-casein, and ${\beta}$-casein, but all the genetic markers related to milk protein decreased significantly after nanoemulsification. Conclusion: Detailed knowledge of factors, such nanogenesis of methionine, associated with increasing cysteine and decreasing production of genetic markers related to milk protein (casein) will help guide future recommendations to producers for maximizing milk yield with a high level of milk protein casein.

• 한국작물학회지
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• 제68권2호
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• pp.47-58
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• 2023

본 연구는 최근 빈번한 고온 노지 재배 피해가 빈번함에 따라 팥 대표 품종 '아라리'의 2021과 2022년 고온구배온실에서 수행된 고온 반응 실험이다. 적정 온도 보다 높은온도 범위에서 팥의 노지 재배시 고온 연구로 국내 품종 '아라리'의 생육 반응, 수량, 화분 활력, 종실 성분 등을 최초로 비교분석 하였다. 1. 세부적인 기상 조건은 2021과 2022년의 최저, 평균, 최고기온 연차간 차이는 2022년 보다 2021년이 전생육기는 0 - 1.0℃로 차이가 적었고 영양생장기 0 - 3.7℃, 개화기는 0.4 - 2.4℃로 높았다. 2021년 보다 2022년에서 등숙기는 최저, 평균 온도는 낮았지만 최고온도는 1.7 - 3.9℃로 높아 연차별 온도에 따라 생육특성이 다르게 나타났다. 2. 연차별 생육기간을 비교해 보면 개화기는 평균 기온차에 의해 2021년 13일, 2022년 27일로 차이가 있었으며 등숙기는 2021년 30일, 2022년 19일로 차이가 있었다. 영양생장기에는 최고 온도가 40.3서도 생육이 양호하였지만 개화기 고온 처리는 평균온도가 27.0℃ 이상이 되면 생육이 불량하여 수량이 낮아지는 경향을 보였다. 2021년에 비해 2022년 등숙기 온도가 최저 온도 1.6 - 1.9℃ 낮고 최고 온도 1.7 - 3.9℃로 높고 기간이 늘어나니 팥의 수량성이 높았다. 3. 전생육기와 영양생장기의 고온 스트레스시 총폴리페놀과 총플라보노이드 함량이 증가하였고 화분 형태와 활력은 대조구와 전생육기T1에서는 통계적으로 차이가 없었지만 T4 (최저온도 22.9℃; 평균온도 28.8℃; 최고온도 36.9℃)에서는 비정상 화분 형태가 45.62%, 급격히 화분 활력이 40.75%로 떨어졌다. 4. 고온 스트레스시 수량은 2021년 개화기 T4 (최저온도 23.6℃; 평균온도 28.5℃; 최고온도 35.8℃)에 2022년은 수량과 화분 임성은 전생육기 T4 (최저온도 22.9℃; 평균온도 28.8℃; 최고온도 36.9℃)에 가장 낮았으며 반면 총폴리페놀과 총플라보노이드는 영양생장기 T4 고온 스트레스가 높은 시기일 때 가장 높았다.

• 한국조경학회지
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• 제30권3호
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• pp.35-45
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• 2002

This research is related to the evaluation of rural amenities. Since the UR negotiations, the wave of market liberalization has brought many difficulties to the rural areas. This paper serves to identify a number of important elements such as socialization, cultural and ecological resources and investigate the viability of rural areas. The purpose of this study is to evaluate rural amenities by evaluation indices and establish planning direction of amenities for rural areas. The data have been collected by methods of a field survey and a questionnaire survey. The evaluation indices have been defined as socialization, safety, amenity and convenience by several researches. Villages have been divided into two groups. Seven(Shimchon, Ibaek, Muan, Dokpyong-li, Imchin-li Hyangyang-li, Wonwol-li) have been newly constructed, six(baeksa, Mokmyon, yongho-li, shindyae-li, hwangdun·songgae) have been redeveloped. There are considerable differences in the degree of satisfaction between he two groups. The results are as follows: 1) socialization is very necessary for amenities in rural villages. Especially the values of the resident's interactions and management of the village are most important in the preparation of an amenity plan in rural areas. So the plans and establishment of public facilities are requisite for the residents community. 2) The convenience of rural public facility plans is necessary for the improvement of the farmers'living conditions. For planning the rural villages, special regards are paid to characteristics of the village, such as the former place of residence and occupations. 3) Newly constructed village should improve their socialization and the redeveloped types should try to get a better life for amenity and safety, The residents of the redeveloped types show relatively high degree of satisfaction with indices of resident community, living convenience facilities, and management of village. On the contrary, amenity and safety are good in newly constructed types. This study has taken into consideration the characteristics of rural villages. It makes a contribution to the redevelopment of rural villages and improves amenities in rural villages. It is recommended that more studies classify the resources of rural areas and measure amenities which are significant to city dwellers in the future.

• 한국작물학회지
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• 제59권2호
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• pp.188-193
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• 2014

Seed viability testing provides valuable information for assessing seed lot germinability. However, most testing methods require destruction of seed prior to test. Because the dissected seeds for viability test cannot be used further evaluation, the nondestructively X-ray photography technique that can be applied for the evaluation of seed quality has been developed. In order to know the validity and accuracy of X-ray photography technique in seed evaluation test that conducted to remove the abnormal seed from a seed lot, we have compared the results from tetrazolium viability test, germination test and X-ray contrast method in cotton. Metallic salts treatment increased the efficiency of X-ray photographic method by enhancing the penetration of X-ray in abnormal or damaged seeds rather than normal seeds that have strong and well-organized tissues in seed. Cotton seeds presoaked for 16 hr in distilled water followed by soaking into metallic salt solution (5% NaI in water) for 60 min were easily classified seeds into dead seed and viable seed based on the radiography images obtained by X-ray radiation. We concluded that soft X-ray photography was reliable to find out the various defective characters due to heat and mechanical damage of seeds.