• Journal of Microbiology and Biotechnology
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• 제16권12호
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• pp.1919-1927
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• 2006

Tumor cells genetically engineered to secrete cytokines are effective in tumor therapy, but various unexpected side effects are observed, which may result from the bulk activation of various bystander cells. In this study, we tested tumor vaccines expressing various membrane-bound forms of IL-2 (mbIL-2) on MethA fibrosarcoma cells to focus antitumor immune responses to CTL. Chimeric forms of IL-2 with whole CD4, deletion forms of CD4, and TNF were expressed on the tumor cell surface, respectively. Tumor clones expressing mbIL-2 or secretory form of IL-2 were able to support the cell growth of CTLL-2, an IL-2-dependent T cell line, and the proliferation of spleen cells from 2C TCR transgenic mice that are responsive to the $p2Ca/L^d$ MHC class I complex. Expression of mbIL-2 on tumor cells reduced the tumorigenicity of tumor cells, and the mice that once rejected the live IL-2/TNF tumor clone acquired systemic immunity against wild-type MethA cells. The IL-2/TNF clone was inferior to other clones in tumor formation, and superior in the stimulation of the CD8+ T cell population in vitro. These results suggest that the IL-2/TNF clone is the best tumor vaccine, and may stimulate CD8+ T cells by direct priming. Expression of IL-2/TNF on tumor cells may serve as an effective gene therapy method to ameliorate the side effects encountered in the recombinant cytokine therapy and the conventional cytokine gene therapy using the secretory form of IL-2.

• 한국수자원학회논문집
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• 제47권10호
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• pp.973-983
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• 2014

본 연구는 밭경지 현장실험을 통해 다양한 초생대 초종을 적용하여 밭의 비점오염저감효과 분석 및 초생대에 적용 가능한 적합한 초종을 선정하고자 하였다. 경북 군위군에 위치한 면적 $1,500m^2$, 경사 5%의 밭경지를 선정하여 시험포를 조성하였으며, 시험포내에는 대조구 1개와 초생대 처리구 6개로 구성된 총 7개의 시험구를 조성하였다. 츄파, 피, 바랭이, 잔디, 톨페스큐, 켄터키블루그라스를 포함하는 6개의 초종을 선정하여 초생대 실험을 수행하였다. 현장실험 결과 대조구 대비 초생대의 유출저감효과는 6.1~77.8%였으며, 비점오염저감효과는 TS의 경우 15.6~90.3%, T-P의 경우 49.9~96.6%, T-N의 경우 6.7~91.1%를 나타내었다. 전체 오염인자 중에 초생대로 인해 전반적으로 높은 비점오염저감 효과를 나타낸 것은 TS, T-P, T-N, SS인 반면, BOD, TOC와 $NO_3^-$는 낮은 저감효과를 보였다. 비점오염저감 및 유지관리측면에서 가장 유리한 초생대 초종은 잔디로 평가되었다. 본 연구결과를 통해 우리나라 농촌지역 밭 비점오염저감을 위한 관리기법으로 초생대가 효과적으로 활용가능하리라 사료되며, 보다 장기적이고 다각적인 현장실험을 통해 초생대 현장적용을 위한 설계 지침서 개발이 요구된다.

• 지역사회간호학회지
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• 제8권1호
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• pp.116-132
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• 1997

The purpose of the study is to find out how laws related to the nursing profession can be improved by analyzing the rules and regulations concerning nursing. Furthermore, to help settle legal matters in the process of doing nursing work. The data used for the study are the Health and Medical Act, the Maternal and Child Health Act, the School Health Act, the Special Act for Health and Medical Service in Rural Areas, the Industrial Health & Safety Act and the Notice on Nursing Professional Courses analyzed by age and content. The results of the study are as follows : First, basic nursing practice includes 'nursing care for recuperation and assistance in medical treatment and in special areas including the pre-vention of disease, maintenance of health, control of environment, and other therapeutic activities. It is suggested that the phrase 'assistance in medical treatment' should be eliminated as it limits the basic nursing practice to the assistance of the medical treatment. Second, Article 56 of the Health & Medical Act prescribes a special nurse but it does not prescribe a specific job. Accordingly, the new provison concerning the specific jobs of a special nurse should be added or a job guide should be inseated. Third, it is prescribed that those who have completed the training course after obtaining a license are qualified to be a midwife, a special nurse and a nurse practitioner working in special areas. However, school nurses, occupational health nurses and maternal and health workers are required to obtain a nurse license, but not to take an additional training course. Nurses working in special areas should be legally recognized as nurse specialists. The regulations to control various qualification standards consistently should be established. Fourth, the qualifications and types of nurses by area prescribed by Article 54 of the Health and Medical Act are not consistent with those of special nurses as recognized by affiliated organizations of the Korean Nurse Association and some hospitals. Accordingly, the qualifications and types of special nurses should be adjusted in consideration of special nurses. Fifth, as Article 16, Paragraph 2 of the Health and Medical Act does not prescribe the type and scope of first - aid treatment that nurses can provide, the first-aid treatment of nurses might be considered as an unlicensed practice. The specific regulations regarding these matters should be established. Sixth, the contents of the nursing record, which are prescribed by Article 21 of the Health and Medical Act as a duty, include 1) matters concerning body temperature, pulse, breath and blood pressure 2) matters concerning drug prescription 3) matters concerning input and output 4) matters concerning the treatment and nursing care (Article 17 of the Enforcement Regulations, Health and Medical Act). However, these matters are limited to basic nursing care and assistance in medical treatment. The new recording methods on nursing process are suggested to be adopted legally. Seventh, the prescription right entrusted to nurses which are prescribed by the School Health Act, the Special Act on Health and Medical Service for Rural Areas, and the Industrial Health and Safety Act are not consistent with the rights of nurses as prescribed by the Health and Medical Act. New regulations prescribing the partial right for medical treatment entrusted to nurses in consideration of the restraint of time and place in emergency situations should be established.

• 한국산업보건학회지
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• 제23권4호
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• pp.402-411
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• 2013

Objectives: This study aims to assess the concentrations of ammonia and hydrogen sulfide according to task unit area at swine farms. Methods: A total of six swine farms were selected for this study. Ammonia and hydrogen sulfide were monitored using a real-time multi-gas monitor which could sample the gases simultaneously. The sampling was done in the pig building, manure storage facility and composting facility of each farm. Results: The concentration of ammonia in the pig buildings(GM 22.6 ppm, GSD 2.3) was significantly higher(p<0.0001) than in the manure storage facilities(GM 10.4 ppm, GSD 2.7) and composting facilities(GM 8.6 ppm, GSD 2.8). The concentration of hydrogen sulfide in the manure storage facilities(GM 9.8 ppm, GSD 3.2) was higher(p<0.0001) than in the pig buildings(GM 2.3 ppm, GSD 2.3) and composting facilities(GM 1.9 ppm, GSD 2.5). In particular, the levels of hydrogen sulfide in the confined manure storage facilities were higher than those in open-type facilities and the peak concentration(98 ppm) in the confined facilities was approximate to 100 ppm, at the value of Immediately Dangerous to Life or Health(IDLH). Conclusions: Suffocation accidents caused by hazardous gases at a swine farm have occurred annually. Real-time monitoring of the hazards should be done in order to protect farm workers and livestock from the sudden accidents.

• 농촌의학ㆍ지역보건
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• 제24권1호
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• pp.1-11
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• 1999

본 연구는 농약에 직업적으로 폭로되고 있는 농부들의 말초신경 기능을 평가하고자 농약살포 남성 농민 34명을 대상으로, 1998년 5월부터 1999년 2월에 걸쳐 실시하였다. 연구내용으로는 말초신경병변을 평가하기 위한 방법으로 현재 가장 많이 사용되고 있는 신경전도 검사를 우세 상하지의 청중신경, 척골신경, 비골신경, 후경골신경, 비복신경 대해 각 구간별로 나누어 실시하였다. 조사결과 조사대상자들은 평균 60세로서 농약살포 경력은 평균 33년이었다. 농사 유형은 주로 논, 밭, 고추 농사를 경작하고 있었으며 년간 평균 약 35일간 농약을 살포하였고 1일 살포시간은 약 9시간이었다. 또한 농약 중독 관련 증상을 경험한 적이 약 82%이상에서 보고되었다. 신경전도 검사상 조사원 모든 신정에서의 값이 정상범위에는 포함되었지만, 일부구간(척골감각 신경의 wrist-elbow, 후경골신경의 terminal latency)을 제외하고는 모두 참고치의 평균값보다 유의하게 낮은 것으로 나타났다. 또한 상대적 고폭로군과 저폭로군 간의 신경전도 검사치 비교에 의하면 정중감각 및 운동, 비골신경의 일부 구간 값이 고폭로군에서 높은 것을 제외하고는 유의한 차이를 발견할 수 없었다. 이것은 고폭로군의 연령이 저폭로군보다 유의하게 낮기 때문으로 판단되며, 조사 대상자들의 값이 참고치 평균보다 떨어진 것도 연령이 높음으로 인한 것임을 연령군별 비교에 의해 확인할 수 있었다. 따라서 향후 농약 폭로로 인한 미세한 말초신경 기의 이상여부를 조기에 파악하기 위해서는 감각역치 검사등 다론 신경학적 검사들을 함께 실시하는 것이 바람직하다고 판단되었다.

• Journal of Preventive Medicine and Public Health
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• 제42권5호
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• pp.315-322
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• 2009

Objectives : Knowledge about the management status of diabetic melitus (DM) is essential to improve diabetic management. Moreover, low income is associated with poor adherence to treatment and increased mortality. This study was performed to evaluate the management status of DM in low-income patients in a rural area. Methods : We enrolled 370 patients with type 2 DM living in Gokseong county, JeollaNamdo. A well-trained examiner measured the height, weight, waist circumference, blood pressure, total cholesterol, triglyceride, high density lipoprotein cholesterol, fasting blood sugar and glycosylated hemoglobin (HbA1c) levels. Carotid ultrasonography was used to measure carotid artery carotid artery intima media thickness (IMT) and plaque. anklebrachial index (ABI) was used to evaluate peripheral artery disease. A fundoscopic examination was performed to evaluate diabetic retinopathy. A history of diabetes complications and health-related questionnaires were also completed. Results : The age of diabetic subjects was 68.7$\pm$8.7 years and the duration of diabetes was 8.9$\pm$8.2 years. Most (63.5%) had hypertension, and 45.7% had triglycerides below 150 mg/dl, 38.1% had low density lipoprotein cholesterol (LDL) cholesterol below 100 mg/dl, 48.7% had urine albumin to creatinine ratio (UACR) below 30 mg/g. Less than half (45.9%) achieved the goal of HbA1c less than 7% suggested by the American Diabetes Association (ADA). 10.6% had peripheral vascular disease, 11.9% had retinopathy, and 60.8% had chronic kidney disease. Conclusions : DM management in low income patients is very poor and requires further work to improve.

• 보건행정학회지
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• 제18권4호
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• pp.66-84
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• 2008

Objectives: This study aimed to. offer some fundamental evidences for the stroke management policy by investigating the trends of medical care utilization and regionalization in stroke inpatients. Methods: We used the National Health Insurance claims and registry data for stroke inpatients from 1998 to 2005. Among all stroke inpatient claims data, self-employed insured and their dependents were only included in this study. The classification of stroke was based on ICD-10(I60-I69) and its subtype was divided by hemorrhage(I60-I62) and infarction(I63-I64) type. To evaluate regionalization of medical care utilization, relevance index was calculated by regions. The regions were classified 8 large catchment areas and 163 self authorized areas. Results: The overall medical care utilization rate of stroke inpatient has been increased, especially infarction subtype. Among medical care institutions, the utilization of hospital has been the most rapidly increased. Although considered annual rate of interest, total medical cost of stroke inpatients has been increased, Totally, more than 84% of stroke inpatient were admitted to medical care institutions in their own large catchment area during 1998-2005. The relevance indices in their own large catchment area (self sufficiency rates) were more than 70% in most areas regardless of stroke subtype except Chungbuk catchment area. Self sufficiency rates of stroke inpatients among 163 self authorized areas in 1998 and 2005 were 84.2% and 83.1% in metropolitan, 46.7% and 45.5% in urban, and 19.5% and 22.6% in rural areas, respectively. Conclusion: Stroke management policy for improvement of distribution at the district level, especially in rural areas, may be helpful for reducing regional inequality in stroke.

• 농촌의학ㆍ지역보건
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• 제35권2호
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• pp.124-133
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• 2010

본 연구는 농촌지역 고등학생 299명을 대상으로 2008년 5월에 구조화된 설문지를 이용하여 정신건강, 우울정도 및 인터넷중독 실태를 파악하고, 제변수간의 관계를 파악하여 청소년 정신건강관리 프로그램을 개발하기 위한 기초자료를 제공하기 위해 실시하였다. 그 결과는 다음과 같다. 첫째, 본 연구 대상자의 정신건강 및 문제행동검사(AMPQ) 결과는 비교적 낮은 문제행동을 나타냈으며, 학교유형별 통계적으로 유의하게 차이가 있게 나온 항목은 외재화 문제와 총문제 행동이었으며, 성별에서 남학생이, 외재화 문제군은 남학생에서 더 문제가 있는 것으로 나왔다. 둘째, 우울정도는 비교적 낮은 점수를 나타냈으며, 잠재적 우울군 5.1%, 고위험 우울군 0.3%이었다. 셋째, 인터넷중독정도는 전체적으로 정상군 96.9%, 잠재적 위험군 1.7%, 고위험군 1.4%이었으며, 제변수간에 통계적으로 유의한 차이는 없었다. 넷째, 대상자의 AMPQ와 우울정도 및 인터넷 중독과는 매우 상관성이 있는 것으로 나왔다. 전반적인 정신건강 문제는 우울정도가 심할수록, 인터넷중독이 심할수록 있는 것으로 나왔다. 이상과 같은 결과에서 청소년 정신건강의 심각성을 인식하고 문제가 발생하기 전에 사전예방하고, 조기에 치료할 수 있는 프로그램의 개발과 제도적 장치 마련이 필요하다. 특히 청소년기 우울증은 성인과 달리 발견이 매우 어려우므로, 가장 중요한 것은 조기 발견하여 적절한 관리 및 치료 방향을 설정하는 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제33권10호
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• pp.1633-1641
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• 2020

Objective: The objective of this study was to develop a model for estimating the carcass weight of Hanwoo cattle as a function of body measurements using three different modeling approaches: i) multiple regression analysis, ii) partial least square regression analysis, and iii) a neural network. Methods: Data from a total of 134 Hanwoo cattle were obtained from the National Institute of Animal Science in South Korea. Among the 372 variables in the raw data, 20 variables related to carcass weight and body measurements were extracted to use in multiple regression, partial least square regression, and an artificial neural network to estimate the cold carcass weight of Hanwoo cattle by any of seven body measurements significantly related to carcass weight or by all 19 body measurement variables. For developing and training the model, 100 data points were used, whereas the 34 remaining data points were used to test the model estimation. Results: The R² values from testing the developed models by multiple regression, partial least square regression, and an artificial neural network with seven significant variables were 0.91, 0.91, and 0.92, respectively, whereas all the methods exhibited similar R² values of approximately 0.93 with all 19 body measurement variables. In addition, relative errors were within 4%, suggesting that the developed model was reliable in estimating Hanwoo cattle carcass weight. The neural network exhibited the highest accuracy. Conclusion: The developed model was applicable for estimating Hanwoo cattle carcass weight using body measurements. Because the procedure and required variables could differ according to the type of model, it was necessary to select the best model suitable for the system with which to calculate the model.

• 한국습지학회지
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• 제16권4호
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• pp.319-325
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• 2014

연못형습지(Pond Wetland)는 우리나라 농업경관에서 많이 분포하고 있다. 이러한 연못형습지는 생물다양성을 위한 중요한 자원으로 평가 받는다. 하지만 연못형습지(Pond Wetland)에 대한 연구는 유형분류에 그치고 있다. 따라서 본 연구는 연못형습지의 생태계서비스(Ecosystem Service) 기능을 알아보았다. 연구방법은 총 3단계로 기능분석, 기능도출, 중요도 분석의 순으로 진행하였다. 선행연구를 분석하여 총 22가지의 기능을 도출하였다. 전문가 설문 결과 22개 항목 중 생물, 환경, 사회문화 분야에 각 4 기능씩 총 12개 기능으로 선별하였다. 항목간 AHP(Analytic Hierarchy Process)를 실시하여 기능별 중요도를 산출하였다. 중복된 기능은 통합하여 연못형습지의 생태계서비스 기능 10가지(1. Water Storage & Irrigation, 2. Vegetation Diversity, 3. Amphibian & Reptile Habitat, 4. Water Purification, 5. Aquatic Insect Habitat, 6. Nutriments Control, 7. Groundwater Recharge, 8. Fishery Habitat, 9. Eco-Experience & Education, 10. Aesthetic landscape)를 도출하였다. 향후 기능별 중요도 분석과 경제적 가치 지표를 적용하여 연못형습지의 생태계서비스 평가에 활용 할 수 있을 것으로 판단된다.