Kim, Se-Woong;Kim, Dae-Hwan;Jung, Yeon-Gil;Roh, Sang-Ho
Reproductive and Developmental Biology
/
v.35
no.1
/
pp.17-22
/
2011
This study was designed to determine the effect of electric field strength, duration and fusion buffer in fusion parameters on the rate of membrane fusion between the somatic cell and cytoplast for Korean cattle (HanWoo) somatic cell nuclear transfer (SCNT) procedure. Following electrofusion, effect of 5 or $10\;{\mu}M$$Ca^{2+}$-ionophore of activation treatment on subsequent development was also evaluated. Cell fusion rates were significantly increased from 23.1% at 20 V/mm to 59.7% at 26 V/mm and 52.9% at 27 V/mm (p<0.05). Due to higher cytoplasmic membrane rupture or cellular lysis, overall efficiency was decreased when the strength was increased to 30 V/mm (18.5%) and 40 V/mm (6.3%) and the fusion rate was also decreased when the strength was at 25 V/mm or below. The optimal duration of electric stimulation was significantly higher in $25\;{\mu}s$ than 20 and $30\;{\mu}s$ (18.5% versus 9.3% and 6.3%, respectively, p<0.05). Two nonelectrolyte fusion buffers, Zimmermann's (0.28 M sucrose) and 0.28 M mannitol solution for cell fusion, were used for donor cell and ooplast fusion and the fusion rate was significantly higher in Zimmermann's cell fusion buffer than in 0.28 M mannitol (91.1% versus 48.4%, respectively, p<0.05). The cleavage and blastocyst formation rates of SCNT bovine embryos activated by $5\;{\mu}M$$Ca^{2+}$-ionophore was significantly higher than the rates of the embryos activated with $10\;{\mu}M$ of $Ca^{2+}$-ionophore (70.0% versus 42.9% and 22.5% versus 14.3%, respectively; p<0.05). This result is the reverse to that of parthenotes which shows significantly higher cleavage and blastocyst rates in $10\;{\mu}M$$Ca^{2+}$-ionophore than $5\;{\mu}M$ counterpart (65.6% versus 40.3% and 19.5% versus 9.7%, respectively; p<0.05). In conclusion, SCNT couplet fusion by single pulse of 26 V/mm for $25\;{\mu}s$ in Zimmermann's fusion buffer followed by artificial activation with $5\;{\mu}M$$Ca^{2+}$-ionophore are suggested as optimal fusion and activation methods in Korean cattle SCNT protocol.
Background: Medtronic-Hall mechanical valve is a world widely using prosthesis. But, in Korea, the clinical result from Medtronic-Hall valve replacement is not frequenthy available. Materials and methods: From March 1986 to May 1990, 50 patients underwent valve replacement surgery with Medtronic-Hall valve at Pusan National University Hospital. Seventeen were male and thirty three were female and ra nging in age from 16 to 70 years of age(mean=35 years). Results: The causes of valvular lesion were rheumatic in 43 patients, bicuspid aortic valve in 3 patients, degenerative lesion in three patients and bacterial endocarditis in one patient. The operative procedures were mitral valve replacement(MVR) in 38, aortic valve replacement(AVR) in 5 and double valve replacement(DVR) in 7. The most commonly used valve size was 21mm in AVR, 29mm in MVR. Concomitant surgical procedures were performed in 15 patients; left atrial thrombectomy in 9, left atrial auricle obliteration in 6 and tricuspid annuloplasty in 5(Kay: 2, DeVega: 3). New York Heart Association functional class was mostly Class Ⅲ or Ⅳ(91.5%) preoperatively and ClassⅠor Ⅱ(87.2%) after operation. The findings of postoperative echocardiogram of LAD, LVESD, LVEDD were reduced compared with preoperative period and ejection fraction was increased compared with preoperative period. Postoperative complications were massive bleeding in three, low cardiac output syndrome in two, thromboembolism in one and fulminant hepatitis in one patient. There were three hospital deaths and their causes were low cardiac output syndrome in two and rupture of left ventricle in one patient. The 5 year survival rate was 93.65±0.71% and 10 year actuarial survival rate was 88.27±6.42%. Conclusions: Medtronic-Hall mechanical valve has low valve related complication rate. It's durability and hemodynamic performance is comparable to other mechanical valves.
An analysis was completed of the hazards distance of hydrogen accidents such as jet release, jet fire, and vapor cloud explosion(VCE) of hydrogen gas, and simplified equations have been proposed to predict the hazard distances to set up safety distance by the gas dispersion, fire, and explosion following hydrogen gas release. For a small release rate of hydrogen gas, such as from a pine-hole, the hazard distance from jet dispersion is longer than that from jet fire. The hazard distance is directly proportional to the pressure raised to a half power and to the diameter of hole and up to several tens meters. For a large release rate, such as from full bore rupture of a pipeline or a large hole of storage vessel, the hazard distance from a large jet fire is longer than that from unconfined vapor cloud explosion. The hazard distance from the fire may be up to several hundred meters. Hydrogen filling station in urban area is difficult to compliance with the safety distance criterion, if the accident scenario of large hydrogen gas release is basis for setting up the safety distance, which is minimum separation distance between the station and building. Therefore, the accident of large hydrogen gas release must be prevented by using safety devices and the safety distance may be set based on the small release rate of hydrogen gas. But if there are any possibility of large release, populated building, such as school, hospital etc, should be separated several hundred meters.
Dong-won, Shin;Min-kyung, Lee;Jeong Hwan, Kim;Ho-seong, Seo;Jae-hun, Lee
Journal of the Korean Institute of Gas
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v.26
no.6
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pp.16-23
/
2022
In this study, characteristics of effect on hydrogen gas was investigated to hydrogen embrittlement by disk and tensile tests. The developed and commercial alloy was fabricated to a plate material made from an alloy ingot. The prepared materials were processed in the form of a disk to measure rupture pressure by hydrogen and helium gas at a rate of 0.1 to 1,000 bar/min. In the hydrogen pre-charged tensile test, a specimen was hydrogenated using an anode charging method, and the yield strength, ultimate tensile strength, elongation, and reduction in area rate were carried by a strain rate test. Also, the microstructure was observed to the fracture surface of the tensile test specimen. As a result, the developed materials satisfied endurable hydrogen embrittlement, and the fractured surface showed a brittleness fracture surface with a depth of several ㎛, but dimple due to ductile fracture could be observed.
Karadag, Burak;Kocak, M.;Kayikcioglu, F.;Ercan, F.;Dilbaz, B.;Kose, M.F.;Haberal, A.
Asian Pacific Journal of Cancer Prevention
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v.15
no.19
/
pp.8489-8493
/
2014
Objective: To verify the basic preoperative evaluation in the discrimination between benign and malignant adnexal masses in our clinical practice. Materials and Methods: Data were collected on the records of 636 women with adnexal masses who had undergone surgery either by open or endoscopic approaches. Those with obvious signs of malignancy, any history of cancer, emergency surgeries without basic evaluation were excluded. The preoperative features by age, ultrasound and serum Ca125 level were compared with final histopathological diagnosis at the four departments of the institution. These are the general gynecology (Group 1: exploratory laparotomy), the gynecologic endoscopy (Group 2: laparoscopy and adnexectomy), the gynecological oncology (Group 3: staging laparotomy) and the gynecologic endocrinology and infertility (Group 4: laparoscopy and cystectomy). Results: There were simple and complex cyst rates of 22.3% and 77.2%, respectively. There were 86.3% benign, 4.1% (n:20) borderline ovarian tumor (BOT) and 6.4% (n:48) malignant lesions. There were 3 BOT and 9 ovarian cancers in Group 1 and one BOT and two ovarian cancer in the Group 2. During the surgery, 15 BOT (75%) and 37 ovarian cancer (77%) were detected in the Group 3, only one BOT was encountered in the Group 4. The risk of rate of unsuspected borderline or focally invasive ovarian cancer significantly increased by age, size, complex morphology and Ca125 (95% CI, OR=2.72, OR=6.60, OR=6.66 and OR=4.69, respectively). Conclusions: Basic preoperative evaluation by comprehensive ultrasound imaging combined with age and Ca125 level has proved highly accurate for prediction of unexpected malignancies. Neither novel markers nor new imaging techniques provide better information that allow clinicians to assess the feasibility of the planned surgery; consequently, the risk of inadvertent cyst rupture during laparoscopy may be significantly decreased in selected cases.
Piao, Cheng Xu;Kim, Tae Hyeung;Yang, Jun Ho;Li, Xiao Yu;Ha, Hyun Chul;Jung, Jae Hun
Journal of Korean Society of Occupational and Environmental Hygiene
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v.18
no.2
/
pp.149-160
/
2008
Bag-filter system has been widely used in industrial field to remove the particulate matters from the exhaust gas. The cylindrical type of bag-filter has been generally used. But it has many shortcomings. The reattachment of separated particles on the surface of bags could result in high pressure drop of bag-filter system and subsequent decrease of air flow rate since the cylindrical type bag-filter system should have the upward flow pattern. In addition, the supply of very high pressure pulse air jet to remove particulate matters on the surface of filter could result in a frequent rupture of bags. To overcome these shortcomings of the cylindrical type, the rectangular type was developed in the developed countries and imported to Korea. But, there was not many design data available to understand the mechanisms. Thus, the fundamental experiments were conducted in this study to get some ideas about the pulse jet cleaning of rectangular type bag filter system. The experimental factors are as follows; pulse distance, pulse duration, pulse interval, pulse pressure and pulse nozzle type. Experiments followed the factorial design method. With the shorter pulse distance, the distribution of pressure drops was relatively not uniform while the particulate removal efficiency was higher. With the longer duration of pulsing and the more number of pulse nozzle, the removal efficiency was higher and the pressure drop distribution was more uniform.
Trauma to the thorax represents a significant portion of injuries seen in an inner-city emergency room. Although most of these patients may be sucessfully managed without thoracotomy, a certain percentage requires operative intervention either immediately or within several hours. 126 records of patients who had early thoracotomy for chest trauma from March 1986, to June 1997, in the Department of Thoracic and Cardiovascular Surgery in Masan Samsung General Hospital were reviewed. There were 96 males and 30 females whose ages ranged from 4 to 72 years, with a mean age of 32.8 years. The modes of injury were as follows : stab wounds, 55 cases(44%), blunt trauma, 70 cases(55%), and gunshot wound, 1 case(1%). Immediate operation was performed in 105 cases(84%) and delayed operation in 21 cases(16%). Indications that operation was necessary were hemorraging and shock in 66 cases(52%), cardiac tamponade in 27 cases(21%), and rupture of the diaphragm in 33 cases(27%). Most of these patients were sucessfully treated but 21 cases were resulted in death. The mortality rate was 16.6% and common causes of death were irreversible shock and hypoxia.
Purpose: To evaluate the integrity of repair & technical tip in use of Massive Cuff Stitch at arthroscopic repair of rotator cuff tear. Materials and Methods: Twenthy-nine cases of arthroscopically repaired full thickness tear of rotator cuffs which are medium sized were evaluated. Between December 2004 to September 2005 we have studied, the average age 53($42{\sim}69$) years old, mean follow-up was 15($12{\sim}21$) months. We analyzed the results statistically by paired t-test. The integrity of repair were verified by follow up MRI which were checked 11 cases and ultrasound which were checked 10 cases. Results: VAS of pain improved preoperative average 7.1 to postoperative 0.9, ADL improved 11.2 to 25.6 respectively, UCLA improved 13.7 to 32.9(all, P<0.05). 89.7% showed excellent & good results at the final follow-up. The satisfied rate was 93.1%(27 cases). There was re-rupture of the repaired rotator cuff in one case out of 11 cases which were checked by MRI, and in one case out of 10 cases which were checked by ultrasound, and there was one case of thinning of cuff which were checked by ultrasound. Conclusion: The use of Massive Cuff Stitch in arthroscopic repair of rotator cuff tear have been reduced technical failure. It will be good surgical technique which maintain the integrity of repairs.
After initial structure of AISI 9260 steel is changed into pearlite and martensite, one is isothermally annealed at $700^{\circ}C$ below of $A_1$ transformation point and the other is isothermally annealed at the same condition after 3 cycles of heating and cooling between $680^{\circ}C$ and $780^{\circ}C$ of $A_1$ transformation point. Analyzing the changes of microstructure, mechanical properties and fractography of tension test, we obtained result as follows. The fastest spheroidization rate by changes of initial structure and heat treatment cycles is appeared at the heat treatment cycle which is isothermally annealed after 3 cycles of heating and cooling at below and above $A_1$ transformation point for martensite. At the above condition, the perfect spheroidization structure is appeared after 60hrs and after then, globular carbide is being coarsened. The mean diameter of globular carbide is $2.4{\times}10^{-3}mm$ after 90hrs. The changes of tension strength during spheroidization heat treatment follows Orwan function, ${\sigma}_o={\sigma}_i+Gb/l$, where l is interspacing of carbide particles and at the above condition, ${\sigma}_o=70.48+2.5{\times}10^{-3}/l(kg/mm^2)$. Fractography of fracture of spheroidization structure in tension test is appeared as dimple which is ductile rupture type by nucleation and growth of void, size of dimple is larger and deeper with increasing of heat treatment time.
The Korean nuclear industry developed the SPACE (Safety and Performance Analysis Code for nuclear power plants) code and this code adpots two-phase flows, two-fluid, three-field models which are comprised of gas, continuous liquid and droplet fields and has a capability to simulate three-dimensional model. According to the revised law by the Nuclear Safety and Security Commission (NSSC) in Korea, the multiple failure accidents that must be considered for accident management plan of nuclear power plant was determined based on the lessons learned from the Fukushima accident. Generally, to improve the reliability of the calculation results of a safety analysis code, verification work for separate and integral effect experiments is required. In this reason, the goal of this work is to verify calculation capability of SPACE code for multiple failure accident. For this purpose, it was selected the experiment which was conducted to simulate a Multiple Steam Generator Tube Rupture(MSGTR) accident with Passive Auxiliary Feedwater System(PAFS) operation by Korea Atomic Energy Research Institute (KAERI) and focused that the comparison between the experiment results and code calculation results to verify the performance of the SPACE code. The MSGR accident has a unique feature of the penetration of the barrier between the Reactor Coolant System (RCS) and the secondary system resulting from multiple failure of steam generator U-tubes. The PAFS is one of the advanced safety features with passive cooling system to replace a conventional active auxiliary feedwater system. This system is passively capable of condensing steam generated in steam generator and feeding the condensed water to the steam generator by gravity. As the results of overall system transient response using SPACE code showed similar trends with the experimental results such as the system pressure, mass flow rate, and collapsed water level in component. In conclusion, it could be concluded that the SPACE code has sufficient capability to simulate a MSGTR accident.
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