• Asian-Australasian Journal of Animal Sciences
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• 제9권1호
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• pp.75-81
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• 1996

The dry matter (DM) disappearance and ruminal solubility of calcium (Ca), phosphorus (P), magnesium (Mg) and potassium (K) in eight Philippine forages were studied. The forages were: paragrass (Brachiaria mutica (Forsk.) Stapf), stargrass (Cynodon plectostachyum Pilger), napiergrass (Pennisetum purpureum Schumach), clopo (Calopogonium mucconoides Desv.), centrocema (Centrocema pubescens Benth.), gliricidia (Gliricidia sepium (Jacq.) Walp.), leucaena (Leucaena leucocephala (Lam.) de Wit.) and sesbania (Sesbania grandiflora (L.) Poir. Nylon bags with samples were incubated for 0, 3, 6, 12, 24, 48 and 72 h in rumen cannulated sheep. The 0-h bags were washed with deionized water. For the 0-h samples, 20.4, 17.2, 50.7, 52.2 and 80.1% of the DM, Ca, P, Mg and K was solubilized, respectively. At 3-h incubation period, DM disappearance was 10 percentage units higher than that of 0-h incubation whereas mineral disappearance increased by 43, 21, 30 and 13% for Ca, P, Mg and K, respectively. At 72-h incubation period, greater proportion of DM, Ca, especially in P, Mg and K was solubilized with a value of 73.8, 71.5, 85.6, 91.4 and 98.2%, respectively. The average particulate passage rate obtained in the present study was 1.9%/h where as the range of disappearance rates of various mineral elements were : 0.4 to 1.2%/h for Ca, 0.1 to 1.6%/h for P, 0.7 to 2%/h for Mg and 0.1 to 2%/h for K. The effective ruminal solubilization (ERS) of the macrominerals was calculated where particulate passage rate and disappearance rate of the various elements were included in the equation. The ERS of Ca, P, Mg and K was 50.0, 72.6, 83.9 and 94.5%, respectively. Species differences (p<0.05) on the various mineral solubilities were also observed. This study shows that ruminal solubility of macrominerals in selected Philippine forages is K > Mg > P > Ca.

• Asian-Australasian Journal of Animal Sciences
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• 제13권5호
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• pp.641-644
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• 2000

In vitro true digestibility of cup-plant (Silphium perfoliatum L.) is higher than other alternative forages and comparative to alfalfa (Medicago sativa L.) even at the high neutral detergent fiber (NDF) concentration. This study was conducted to determine whether the digestion kinetic parameters of cup-plant could explain high in vitro true digestibility of cup-plant at the several NDF levels. Cup-plant and alfalfa were both collected in Arlington and Lancaster, Wisconsin to meet the NDF content within 40 to 50% range. The collected samples were incubated with rumen juice to investigate the digestion kinetics at 3, 6, 9, 14, 20, 28, 36, 48, and 72 h. Kinetics was estimated by the model $R=D_0\;e-k(t-L)+U$ where R is residue remaining at time t, and $D_0$ is digestible fraction, k is digestion rate constant, L is discrete lag time, and U is indigestible fraction. Parameters of the model were estimated by the direct nonlinear least squares (DNLS) method. Digestion rate and potential extent of digestion were not statistically different in either forage. However, alfalfa had shorter lag time (p<0.05). The indigestible fraction increased with maturation in alfalfa and in cup-plant (p<0.05). The ratio of indigestible fraction to acid detergent lignin (ADL) was higher in cup-plant than in alfalfa (p<0.05). From the results, alfalfa is probably digested more rapidly than cup-plant, however, cup-plant maintains higher digestibility with maturation due to a relatively slower increase of indigestible fraction in NDF.

• Asian-Australasian Journal of Animal Sciences
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• 제17권1호
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• pp.53-62
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• 2004

The aim of this study was to predict the energy value and dynamic degradation of roughage in Taiwan using the $Daisy^{(R)}$. in vitro fermentation method to provide information on one of the very important nutrients for ration formulation. The second objective was to study the effects of Aspergillus oryzae (AFE) inclusion on nutrient utilization. Three ruminal fistulated dry dairy cows were used for rumen fluid and fifteen conventional forages used in dairy cattle were collected around this island. The degradability of these feedstuffs with and without AFE ($Amaferm^{(R)}$.) treatment was measured using the $Daisy^{(R)}$. in vitro method. The roughage energy values, including TDN and NEL, were calculated according to Robinson (2000). Results from the 30 h in vitro neutral detergent fiber (NDF) degradability and predicted energy evaluations showed that alfalfa (among the forages) contained the highest degradability and energy values, Bermuda straw having the lowest. Peanut vines and corn silage contained higher energy values and the lowest value found in Pangola and Napier grasses among the locally produced forages. Pangola and Napier grasses had lower values than most imported forages except Bermuda straw. Among the by-products, wheat middling contained the highest NDF degradability, while rice bran contained the richest energy value due to its high oil content. From the dynamic dry matter (DM), organic matter (OM), acid detergent fiber (ADF) and neutral detergent fiber (NDF) degradation, corn silage contained the highest effective degradation among the local forages; wheat middling (among the by-products) degraded the fastest in DM, OM, ADF and NDF and showed the highest effective degradability. AFE inclusion was inconsistent among the forages. Alfalfa hay showed significantly increased 30 h NDF degradability and energy values, Pangola hay, Napier grass and brewer's grains showed decreased degradability and energy values. AFE inclusion increased the DM, OM and NDF degradation rate in most forage, but only increased the DM degradation rate in sorghum distiller's grains, the OM degradation rate in bean curd pomace and the NDF and ADF degradation rates in soy pomace (among the by-products).

• Asian-Australasian Journal of Animal Sciences
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• 제4권1호
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• pp.59-65
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• 1991

From the same harvest of Italian ryegrass (Lolium multiflorum, Lam.), hay(H), wilted silage(WS), pressed cake hay (PCH) and pressed cake silage (PCS) were prepared. These four preserved roughages were restrictedly fed to four goats attached with rumen fistula by $4{\times}4$ Latin square design to determine the effect of different physical and chemical properties of the roughages on the ruminal acid concentrations. Each goat was given a diet at 2% of the body weight daily in dry matter basis by separating into two equal portions. Half was given at 9 AM and the other half at 5 PM. Ruminal pH was reduced to around 5.5 within 30 minutes after feeding PCS and it was recovered above 6 in 1-2 hours after feeding. By feeding WS, ruminal pH was also reduced but never fell below 6. The two hays rather increased ruminal pH after feeding. The reduction of ruminal pH in the silage feedings was due to the high lactic acid content of the silages, because the highest ruminal lactic acid concentration was observed 30 minutes after feeding when the lowest ruminal pH was attained. While the ruminal VFA concentrations became the highest 1-2 hours after feeding. The ruminal acetic acid concentration fluctuated so much that no significant tendency was observed among the four dietary treatments. The ruminal propionic acid concentration was higher in feeding silages reflecting the initial high lactic acid concentration. As the result, acetic/propionic acid ratio was lower in the silage feedings than in hay feedings. Higher ruminal butyric acid concentration was observed in WS than in others.

• Journal of Animal Science and Technology
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• 제61권1호
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• pp.18-27
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• 2019

With South Korea's limited capability of feed production because of its relatively small cultivable area, the country is pushed to depend on foreign feed imports despite the immensely fluctuating price of corn. Hence, intensive efforts to increase the total cultivable area in Korea like extending of farming to mountainous area is being practiced. Corn was planted in Goesan County, a mountainous area in the country. Grain and stover were harvested separately in three harvest periods: early-harvest (Aug 8), mid-harvest (Aug 18), and late-harvest (Aug 28). The nutrient composition such as dry matter (DM), crude protein (CP), crude fat (EE), organic matter (OM), neutral detergent fiber (NDF), acid detergent fiber (ADF), and non-fibrous carbohydrates (NFC) was determined after harvest. Effective degradability (ED) of the major nutrients (DM, NDF, ADF, and CP) were measured through in vitro fermentation of rumen fluid from Hanwoo (Korean cattle). pH, ammonia-N concentration, volatile fatty acid (VFA) concentration, and gas production were periodically measured at 0, 3, 6, 12, 24, 48, and 72 h. Corn grain showed higher nutrient content and ED than stover. It also had higher gas production but its pH, ammonia-N, and total VFA concentration were lower than corn stover. The best nutrient composition of corn grain was observed in early-harvest (high CP, EE, NDF, OM, NFC, and low ADF). Early-harvest of corn grain also had high effective degradability of dry matter (EDDM), effective degradability of neutral detergent fiber (EDNDF), effective degradability of acid detergent fiber (EDADF), and total VFA concentration. On the other hand, the best nutrient composition of stover was observed in mid-harvest (high DM, CP, NDF, and low ADF). EDDM, EDNDF, and EDADF were pronounced in early-harvest and mid-harvest of stover but the latter showed high total VFA concentration. Hence, early and mid-harvested corn stover and grain in a mountainous area preserved their nutrients, which led to the effective degradation of major nutrients and high VFA production.

• Animal Bioscience
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• 제34권1호
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• pp.36-47
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• 2021

Objective: This study determined the optimal ratio of whole plant corn silage (WPCS) to corn stover (stems+leaves) silage (CSS) (WPCS:CSS) to reach the greatest profit of dairy farmers and evaluated its consequences with corn available for other purposes, enteric methane production and milk nitrogen efficiency (MNE) at varying milk production levels. Methods: An optimization model was developed. Chemical composition, rumen undegradable protein and metabolizable energy (ME) of WPCS and CSS from 4 cultivars were determined to provide data for the model. Results: At production levels of 0, 10, 20, and 30 kg milk/cow/d, the WPCS:CSS to maximize the profit of dairy farmers was 16:84, 22:78, 44:56, and 88:12, respectively, and the land area needed to grow corn plants was 4.5, 31.4, 33.4, and 30.3 ha, respectively. The amount of corn available (ton DM/ha/yr) for other purposes saved from this land area decreased with higher producing cows. However, compared with high producing cows (30 kg/d milk), more low producing cows (10 kg/d milk) and more land area to grow corn and soybeans was needed to produce the same total amount of milk. Extra land is available to grow corn for a higher milk production, leading to more corn available for other purposes. Increasing ME content of CSS decreased the land area needed, increased the profit of dairy farms and provided more corn available for other purposes. At the optimal WPCS:CSS, MNE and enteric methane production was greater, but methane production per kg milk was lower, for high producing cows. Conclusion: The WPCS:CSS to maximize the profit for dairy farms increases with decreased milk production levels. At a fixed total amount of milk being produced, high producing cows increase corn available for other purposes. At the optimal WPCS:CSS, methane emission intensity is smaller and MNE is greater for high producing cows.

• Asian-Australasian Journal of Animal Sciences
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• 제29권2호
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• pp.159-169
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• 2016

The goats raised in the barn are usually fed on fresh grass. As dry forage can be stored for long periods in large amounts, dry forage feeding makes it possible to feed large numbers of goats in barns. This review explains the physiological factors involved in suppressing dry forage intake and the cause of drinking following dry forage feeding. Ruminants consume an enormous amount of dry forage in a short time. Eating rates of dry forage rapidly decreased in the first 40 min of feeding and subsequently declined gradually to low states in the remaining time of the feeding period. Saliva in large-type goats is secreted in large volume during the first hour after the commencement of dry forage feeding. It was elucidated that the marked suppression of dry forage intake during the first hour was caused by a feeding-induced hypovolemia and the loss of $NaHCO_3$ due to excessive salivation during the initial stages of dry forage feeding. On the other hand, it was indicated that the marked decrease in feed intake observed in the second hour of the 2 h feeding period was related to ruminal distension caused by the feed consumed and the copious amount of saliva secreted during dry forage feeding. In addition, results indicate that the marked decreases in dry forage intake after 40 min of feeding are caused by increases in plasma osmolality and subsequent thirst sensations produced by dry forage feeding. After 40 min of the 2 h dry forage feeding period, the feed salt content is absorbed into the rumen and plasma osmolality increases. The combined effects of ruminal distension and increased plasma osmolality accounted for 77.6% of the suppression of dry forage intake 40 min after the start of dry forage feeding. The results indicate that ruminal distension and increased plasma osmolality are the main physiological factors in suppression of dry forage intake in large-type goats. There was very little drinking behavior observed during the first hour of the 2 h feeding period most water consumption occurring in the second hour. The cause of this thirst sensation during the second hour of dry forage feeding period was not hypovolemia brought about by excessive salivation, but rather increases in plasma osmolality due to the ruminal absorption of salt from the consumed feed. This suggests the water intake following dry forage feeding is determined by the level of salt content in the feed.

• Asian-Australasian Journal of Animal Sciences
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• 제25권2호
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• pp.240-247
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• 2012

Dietary supplementation with conventional linted cottonseed hulls (LCSH) is a common practice in livestock production all over the world. However, supplementation with mechanically delinted cottonseed hulls (DCSH) and cottonseed linter residue (CLR) is uncommon. Cottonseed by-products, including LCSH, DCSH and CLR, were assessed by chemical analysis, an in situ nylon bag technique, an in vitro cumulative gas production technique and in vitro enzyme procedure. The crude protein (CP) content of CLR (302 g/kg dry matter (DM)) was approximately 3 times that of LCSH and 5 times that of DCSH. The crude fat content was approximately 3 times higher in CLR (269 g/kg DM) than in LCSH and 4 times higher than in DCSH. Neutral detergent fibre (311 g/kg DM) and acid detergent fibre (243 g/kg DM) contents of CLR were less than half those of DCSH or LCSH. Metabolisable energy, estimated by in vitro gas production and chemical analyses, ranked as follows: CLR (12.69 kJ/kg DM)>LCSH (7.32 kJ/kg DM)>DCSH (5.82 kJ/kg DM). The in situ degradation trial showed that the highest values of effective degradability of DM and CP were obtained for CLR (p<0.05). The in vitro disappearance of ruminal DM ranked as follows: CLR>LCSH>DCSH (p<0.05). The lowest digestibility was observed for DCSH with a two-step in vitro digestion procedure (p<0.05). The potential gas production in the batch cultures did not differ for any of the three cottonseed by-product feeds. The highest concentration of total volatile fatty acids was observed in CLR after a 72 h incubation (p<0.05). The molar portions of methane were similar between all three treatments, with an average gas production of 22% (molar). The CLR contained a higher level of CP than did LCSH and DCSH, and CLR fermentation produced more propionate. The DCSH and LCSH had more NDF and ADF, which fermented into greater amounts of acetate.

• 한국축산식품학회지
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• 제31권3호
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• pp.477-483
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• 2011

본 연구는 유가공품에 적용할 수 있는 트랜스 지방산 분석조건을 확립하고 우리나라로 수입되는 유제품의 트랜스 지방산 함량 수준을 확인하기 위해 실시되었다. 조지방 추출은 Rose-Gottlieb법으로 조제분유 표준인증물질을 분석하고 그 인증값과 비교하였던 바, CODEX 가이드에 부합되는 정확도를 나타냈다. 유제품의 트랜스 지방산 분석을 위해 가스크로마토그래프 오븐 온도 상승 조건을 다양하게 변형하여, 트랜스 지방산들이 양호한 분해능을 보이면서 분석되는 적합한 온도 조건을 제시하였고, 최적화된 분석법을 사용하여 우리나라로 수입되는 유가공품(13개 유형, 45제품)에 적용하였다. 수입되는 유제품의 트랜스 지방산 함량은 제품 100 g 당 0.1-5.4 g 범위를 나타내었다. 가장 많이 수입이 되는 치즈류는 0.1-2.4 g/100 g 제품(평균값 1.1 g/100 g, n=35)으로 확인된 반면, 치즈를 제외한 유가공품은 1.7-5.4 g/100 g 제품 수준이었다. 이러한 트랜스 지방산 함량은 반추동물의 제1위 미생물에 의해서 생성되어 우유 중에 존재하는 트랜스 지방산 함량수준이며, 원료로 사용되는 우유의 트랜스 지방산 함량이나 부가적으로 첨가되는 식물성 지방에서 유래되는 트랜스 지방산의 함량에 따라 검사대상 유제품에서 함량의 차이는 편차가 있을 것으로 평가하였다

• Asian-Australasian Journal of Animal Sciences
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• 제9권5호
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• pp.557-562
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• 1996

The effects of diets differing in protein content through soybean meal supplementation on ruminal fractionation of selenium (Se) were studied. A $3{\times}3$ Latin square design was used with three Japanese Corriedale wethers (45 kg average body weight), three periods, and three dietary treatment. The three dietary treatments were : Diet 1, without soybean meal supplementation (14% crude protein, CP); Diet 2, with 10% soybean meal supplementation (16.5% CP); and Diet 3, with 20% soybean meal supplementation (19% CP). All the diets had a Se supplementation in the form of sodium selenite at 0.2 mg Se/kg dietary DM. The Se supplement and the concentrate mixture were fed only in the morning before the hay was given. Daily feeding schedule for gay was set at 09:00 and 17:00 h. On the final day of collection period, ruminal fluid samples were obtained at 0.5, 2, 6, 12 and 24 h post-feeding starting at 09:00 h. Total ruminal fluid Se was markedly higher (p<0.05) in Diet 3 than those in Diets 1 and 2 at almost all sampling time except at 24 h. The proportion of Se in soluble protein to the total ruminal Se was higher (p< 0.05) in Diet 3 (40%) followed by Diet 2 (28%) and Diet 1 (21%). The proportion of free inorganic Se to the total ruminal Se was the reverse, especially after two hours where Diet 1 (p<0.05) was higher than the other diets. Bacterial Se was lower (p < 0.05) in Diet 1 than those in Diets 2 and 3 at any sampling time. The highest was observed at 2 h postprandially in all diets with a value of 421, 556, $655{\mu}g/kg$ bacterial DM for Diet 1, 2 and 3, respectively. No differences (p>0.05) were observed on ruminal pH, ammonia and total nolatile fatty acids although increasing protein supplementation tended to decline the ruminal pH and increase ruminal ammonia. This study concludes that increasing dietary protein content by soybean meal supplementation can affect the ruminal Se metabolism.