• Journal of Microbiology and Biotechnology
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• 제21권4호
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• pp.412-420
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• 2011

Secretion of cellobiase occurred in a brefeldin A (BFA) uninhibited manner in the filamentous fungus Termitomyces clypeatus. Fluorescence confocal microscopy revealed that application of the drug at a concentration of 50 ${\mu}g$/ml caused arrest of Spitzenkorper assembly at the hyphal tip. This resulted in greater than 30% inhibition of total protein secretion in the culture medium. However, the cellobiase titer increased by 17%, and an additional 13% was localized in the vacuolar fraction en route secretion. The secretory vacuoles formed in the presence of the drug were also found to be bigger (68 nm) than those in the control cultures (40 nm). The enzyme secreted in the presence and absence of BFA revealed a single activity band in both cases in native PAGE and had similar molecular masses (approx. 120 kDa) in SDS-PAGE. The BFA enzyme retained 72% of native glycosylation. It also exhibited a higher stability and retained 98% activity at $50^{\circ}C$, 93.3% activity at pH 9, 63.64% activity in the presence of 1M guanidium hydrochloride, and 50% activity at a glucose concentration of 10 mg/ml in comparison to 68% activity, 75% activity, 36% activity, and 19% activity for the control enzyme, respectively. The observations collectively aimed at the operation of an alternative secretory pathway, distinct from the target of brefeldin A, which bypassed the Golgi apparatus, but still was able to deliver the cargo to the vacuoles for secretion. This can be utilized in selectively enhancing the yield and stability of glycosidases for a successful industrial recipe.

• 한국소음진동공학회논문집
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• 제22권4호
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• pp.307-317
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• 2012

The LRT(light rail transit) system, which has medium transport capacity between subway and bus is an advanced transportation system. It has many benefits like chap construction and operational cost down through driverless and flexible route planning. The rubber tired AGT(K-AGT) is a kind of LRT, which has rubber wheels and side guides. The side guides has many advantages, but brings vibration and noise problems from the friction between the guide rail and the wheels. This is the major source for the of passengers' discomfort. The purpose of this paper is to analyze dynamic characteries and running stability of the rubber tired AGT localization bogie if the AGT's speed is increased from 70 km/h to 80 km/h. The current design parameters of bogie suspension, as it is designed, was examined to satisfy the comfort index of the railway vehicle in performance test.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제11권12호
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• pp.5729-5744
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• 2017

Vehicular Ad Hoc Networks (VANET) in the large-scale road section usually have typical characteristics of large number of vehicles and unevenly distribution over geographic spaces. These two inherent characteristics lead to the unsatisfactory performance of VANETs. This poor performance is mainly due to fragile communication link and low dissemination efficiency. We propose a novel routing mechanism to address the issue in the paper, which includes a competition-based routing discovery with priority metrics and a local routing repair strategy. In the routing discovery stage, the algorithm uses adaptive scheme to select a stable route by the priorities of routing metrics, which are the length of each hop, as well as the residual lifetime of each link. Comparisons of different ratios over link length and link stability further show outstanding improvements. In the routing repair process, upstream and downstream nodes also compete for the right to establish repair process and to remain as a member of the active route after repair. Our simulation results confirm the improved performance of the proposed algorithm.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2012년도 춘계학술대회
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• pp.241-244
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• 2012

모바일 애드혹 네트워크에서 노드들은 한정된 에너지 자원을 가지고 있기 때문에 에너지 효율적인 라우팅 기법을 통해 통신 에너지를 절약하는 것이 가장 중요하다. 기존의 클러스터 기반 라우팅 프로토콜은 클러스터 헤드가 통신의 대부분에 참여하게 된다. 따라서 클러스터 헤드의 수명이 줄어들고 효율적인 경로를 가지지 못하게 된다. 본 논문에서 제안하는 새로운 클러스터 기반 라우팅 프로토콜은 클러스터 헤드가 멤버 노드들에게 경로를 알려주는 제어 패킷을 전송한다. 따라서 클러스터 헤드의 통신 참여가 줄어들고, 멤버 노드들은 반드시 클러스터 헤드를 거쳐 통신하는 과정을 생략하게 되므로 클러스터 헤드의 수명이 연장되고 효율적인 경로를 가지게 된다. 본 논문에서 제안한 프로토콜은 기존 클러스터 기반 라우팅 프로토콜과 비교하여 클러스터의 수명, 라우팅의 효율성, 경로의 안정성이 향상 되었다.

• 한국정보전자통신기술학회논문지
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• 제13권2호
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• pp.129-137
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• 2020

현재 자율주행 차량 시장은 3레벨 자율주행 차량의 상용화를 넘어 4레벨 자율주행 차량을 연구, 개발하고 있다. 4레벨 자율주행 차량에서 가장 주목되는 부분은 차량의 안정성이다. 3레벨과 다르게 4레벨의 자율주행 차량은 긴급상황을 차량이 직접 대처해야 하기 때문이다. 본 논문에서는 긴급상황에서의 즉각적인 반응보다는 차량의 목적지가 정해진 순간 사고 가능성이 가장 낮은 경로를 결정하는 Optimized Vehicle Routing System (OVRS)을 제안한다. OVRS는 RSU 통신으로 수집한 도로와 주변 차량 정보를 분석하여 도로의 위험성을 예측하여 주행 중인 차량이 더 안전하고 빠른 길로 주행할 수 있도록 경로를 설정한다. OVRS는 네트워크 라우팅 방식처럼 도로에 있는 RSU를 통하여 도로 상황에 따른 경로 안내를 실행하기 때문에 차량의 안정성을 더욱 높일 수 있다. 실험 결과, OVRS모듈 중 하나인 ASICM의 RPNN은 CNN보다 약 17%, LSTM보다 약 40% 더 좋은 연산 시간을 보였다. 그러나 해당 연구가 PC를 이용한 가상환경에서 실행되었기 때문에, VPDM의 사고 가능성을 실제로 검증하지 못했다. 따라서 향후 사고 데이터 수집으로 인한 VPDM의 정확도 높은 실험과 실제 차량 및 RSU에서 실제 도로를 대상으로 한 실험이 진행되어야 한다.

• 한국전기전자재료학회논문지
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• 제31권3호
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• pp.141-145
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• 2018

Ni-InGaAs shows promise as a self-aligned S/D (source/drain) alloy for n-InGaAs MOSFETs (metal-oxide-semiconductor field-effect transistors). However, limited thermal stability and instability of the microstructural morphology of Ni-InGaAs could limit the device performance. The in situ deposition of a Pd interlayer beneath the Ni layer was proposed as a strategy to improve the thermal stability of Ni-InGaAs. The Ni-InGaAs alloy layer prepared with the Pd interlayer showed better surface roughness and thermal stability after furnace annealing at $570^{\circ}C$ for 30 min, while the Ni-InGaAs without the Pd interlayer showed degradation above $500^{\circ}C$. The Pd/Ni/TiN structure offers a promising route to thermally immune Ni-InGaAs with applications in future n-InGaAs MOSFET technologies.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제8권12호
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• pp.4411-4431
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• 2014

Mobile ad hoc networks (MANETs) have recently gained increased interest due to the widespread use of smart mobile devices. Group communication applications, serving for better cooperation between subsets of business members, become more significant in the context of MANETs. Multicast routing mechanisms are very useful communication techniques for such group-oriented applications. This paper deals with multicast routing problems in terms of stability and scalability, using the concept of stable core. We propose LMRSC (Lightweight Multicast Routing Based on Stable Core), a lightweight multicast routing technique for MANETs, in order to avoid periodic flooding of the source messages throughout the network, and to increase the duration of multicast routes. LMRSC establishes and maintains mesh architecture for each multicast group member by dividing the network into several zones, where each zone elects the most stable node as its core. Node residual energy and node velocity are used to calculate the node stability factor. The proposed algorithm is simulated by using NS-2 simulation, and is compared with other multicast routing mechanisms: ODMRP and PUMA. Packet delivery ratio, multicast route lifetime, and control packet overhead are used as performance metrics. These metrics are measured by gradual increase of the node mobility, the number of sources, the group size and the number of groups. The simulation performance results indicate that the proposed algorithm outperforms other mechanisms in terms of routes stability and network density.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.317.2-317.2
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• 2013

Ultrathin oxide encapsulated metal-oxide hybrid nanocatalysts have been fabricated by a soft chemical and facile route. First, SiO2 nanoparticles of 25~30 nm size have been synthesized by modified Stobber's method followed by amine functionalization. Metal nanoparticles (Ru, Rh, Pt) capped with polymer/citrate have been deposited on functionalized SiO2 and finally an ultrathin layer of TiO2 coated on surface which prevents sintering and provides high thermal stability while maximizing the metal-oxide interface for higher catalytic activity. TEM studies confirmed that 2.5 nm sized metal nanoparticles are well dispersed and distributed throughout the surface of 25 nm SiO2 nanoparticles with a 3-4 nm TiO2 ultrathin layer. The metal nanoparticles are still well exposed to outer surface, being enabled for surface characterization and catalytic activity. Even after calcination at $600^{\circ}C$, the structure and morphology of hybrid nanocatalysts remain intact confirm the high thermal stability. XPS spectra of hybrid nanocatalyst suggest the metallic states as well as their corresponding oxide states. The catalytic activity has been evaluated for high temperature CO oxidation reaction as well as photocatalytic H2 generation under solar simulation. The design of hybrid structure, high thermal stability, and better exposure of metal active sites are the key parameters for the high catalytic activity. The maximization of metal-TiO2 interface interaction has the great role in photocatalytic H2 production.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2005년도 지반공학 공동 학술발표회
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• pp.601-612
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• 2005

In mountainous area, Two parallel tunnels have been usually recognized as a road tunnel which has benefits in aspects of cost and stability. However, Design and construction of 2-Arch road tunnel are growing recently due to environmental destruction, compensation of land and difficulty of route separation. As studies are mainly undergoing on only guaranteeing stability and developing a waterproofing-drainage system to avoid water leakage through comprehension for characteristics of 2-arch tunnel behaviors, there is a tendency to evaluate quantity of support by empirical method with a tunnel which has a complicated cross-section and lack of construction ability. In this study, therefore, we made a plan of tunnel cross-section which had shown good construction ability and developed the waterproofing-drainage system which is able to solve the water leakage problem fundamentally by analyzing precedented 2-arch tunnels and investigating their sites in and out of nation. We also determined fixed quantity of support by a large-scale model test and numerical analysis. We want to contribute to 2-arch tunnel design hereafter introducing design procedure and method applied here.

• 융합보안논문지
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• 제14권7호
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• pp.37-43
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• 2014

오늘날에는 기후, 전파천문, 고성능 컴퓨팅 자원 공동 활용 등의 첨단 기술을 요구하는 그룹별 연구 활동이 강화되고 있다. 이에 따라 이와 같은 오늘날의 첨단 응용 연구를 뒷받침하기 위해 첨단 연구망 역시 첨단 응용연구를 위한 수단으로서 점차적으로 자리매김하고 있다. 따라서 이러한 추세를 반영해서 첨단 연구망 네트워크의 관리적 측면 역시 집중적으로 조명되어야 할 필요성이 있다. 그렇기 때문에, 본 논문에서는 제시하고자 하는 가장 큰 맥락에서의 목표가 '여러 첨단 응용 연구의 도구적 수단으로서의 네트워크를 안정적으로 관리할 수 있도록 하는 연구'이다.