• Journal of Microbiology and Biotechnology
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• v.21 no.4
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• pp.412-420
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• 2011

Secretion of cellobiase occurred in a brefeldin A (BFA) uninhibited manner in the filamentous fungus Termitomyces clypeatus. Fluorescence confocal microscopy revealed that application of the drug at a concentration of 50 ${\mu}g$/ml caused arrest of Spitzenkorper assembly at the hyphal tip. This resulted in greater than 30% inhibition of total protein secretion in the culture medium. However, the cellobiase titer increased by 17%, and an additional 13% was localized in the vacuolar fraction en route secretion. The secretory vacuoles formed in the presence of the drug were also found to be bigger (68 nm) than those in the control cultures (40 nm). The enzyme secreted in the presence and absence of BFA revealed a single activity band in both cases in native PAGE and had similar molecular masses (approx. 120 kDa) in SDS-PAGE. The BFA enzyme retained 72% of native glycosylation. It also exhibited a higher stability and retained 98% activity at $50^{\circ}C$, 93.3% activity at pH 9, 63.64% activity in the presence of 1M guanidium hydrochloride, and 50% activity at a glucose concentration of 10 mg/ml in comparison to 68% activity, 75% activity, 36% activity, and 19% activity for the control enzyme, respectively. The observations collectively aimed at the operation of an alternative secretory pathway, distinct from the target of brefeldin A, which bypassed the Golgi apparatus, but still was able to deliver the cargo to the vacuoles for secretion. This can be utilized in selectively enhancing the yield and stability of glycosidases for a successful industrial recipe.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.22 no.4
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• pp.307-317
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• 2012

The LRT(light rail transit) system, which has medium transport capacity between subway and bus is an advanced transportation system. It has many benefits like chap construction and operational cost down through driverless and flexible route planning. The rubber tired AGT(K-AGT) is a kind of LRT, which has rubber wheels and side guides. The side guides has many advantages, but brings vibration and noise problems from the friction between the guide rail and the wheels. This is the major source for the of passengers' discomfort. The purpose of this paper is to analyze dynamic characteries and running stability of the rubber tired AGT localization bogie if the AGT's speed is increased from 70 km/h to 80 km/h. The current design parameters of bogie suspension, as it is designed, was examined to satisfy the comfort index of the railway vehicle in performance test.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.12
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• pp.5729-5744
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• 2017

Vehicular Ad Hoc Networks (VANET) in the large-scale road section usually have typical characteristics of large number of vehicles and unevenly distribution over geographic spaces. These two inherent characteristics lead to the unsatisfactory performance of VANETs. This poor performance is mainly due to fragile communication link and low dissemination efficiency. We propose a novel routing mechanism to address the issue in the paper, which includes a competition-based routing discovery with priority metrics and a local routing repair strategy. In the routing discovery stage, the algorithm uses adaptive scheme to select a stable route by the priorities of routing metrics, which are the length of each hop, as well as the residual lifetime of each link. Comparisons of different ratios over link length and link stability further show outstanding improvements. In the routing repair process, upstream and downstream nodes also compete for the right to establish repair process and to remain as a member of the active route after repair. Our simulation results confirm the improved performance of the proposed algorithm.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2012.05a
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• pp.241-244
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• 2012

In mobile ad-hoc networks, it should be the most important issue to reduce the power consumption of communications, because the resource of a node is limited in these networks. In existing cluster-based routing protocols, cluster headers participate in almost all communication processes. Therefore the lifetime of the cluster header is shortened and it does not have the effective route. In the proposed cluster-based routing protocol, the cluster header transmits a control packet which gives the route information to member nodes. This makes that the cluster header decreases the number of participating in communications, and that node members do not have to communicate trough the cluster header. This results in extending the lifetime of the cluster header, and having the effective route, data transmission rate and improved stability of routes.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.13 no.2
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• pp.129-137
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• 2020

Currently, The autonomous vehicle market is researching and developing four-level autonomous vehicles beyond the commercialization of three-level autonomous vehicles. Because unlike the level 3, the level 4 autonomous vehicle has to deal with an emergency directly, the most important aspect of a four-level autonomous vehicle is its stability. In this paper, we propose an Optimized Vehicle Routing System (OVRS) that determines the route with the lowest probability of an accident at the destination of the vehicle rather than an immediate response in an emergency. The OVRS analyzes road and surrounding vehicle information collected by The RSU communication to predict road hazards, and sets the route for the safer and faster road. The OVRS can improve the stability of the vehicle by executing the route guidance according to the road situation through the RSU on the road like the network routing method. As a result, the RPNN of the ASICM, one of the OVRS modules, was about 17% better than the CNN and 40% better than the LSTM. However, because the study was conducted in a virtual environment using a PC, the possibility of accident of the VPDM was not actually verified. Therefore, in the future, experiments with high accuracy on VPDM due to the collection of accident data and actual roads should be conducted in real vehicles and RSUs.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.31 no.3
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• pp.141-145
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• 2018

Ni-InGaAs shows promise as a self-aligned S/D (source/drain) alloy for n-InGaAs MOSFETs (metal-oxide-semiconductor field-effect transistors). However, limited thermal stability and instability of the microstructural morphology of Ni-InGaAs could limit the device performance. The in situ deposition of a Pd interlayer beneath the Ni layer was proposed as a strategy to improve the thermal stability of Ni-InGaAs. The Ni-InGaAs alloy layer prepared with the Pd interlayer showed better surface roughness and thermal stability after furnace annealing at $570^{\circ}C$ for 30 min, while the Ni-InGaAs without the Pd interlayer showed degradation above $500^{\circ}C$. The Pd/Ni/TiN structure offers a promising route to thermally immune Ni-InGaAs with applications in future n-InGaAs MOSFET technologies.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.8 no.12
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• pp.4411-4431
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• 2014

Mobile ad hoc networks (MANETs) have recently gained increased interest due to the widespread use of smart mobile devices. Group communication applications, serving for better cooperation between subsets of business members, become more significant in the context of MANETs. Multicast routing mechanisms are very useful communication techniques for such group-oriented applications. This paper deals with multicast routing problems in terms of stability and scalability, using the concept of stable core. We propose LMRSC (Lightweight Multicast Routing Based on Stable Core), a lightweight multicast routing technique for MANETs, in order to avoid periodic flooding of the source messages throughout the network, and to increase the duration of multicast routes. LMRSC establishes and maintains mesh architecture for each multicast group member by dividing the network into several zones, where each zone elects the most stable node as its core. Node residual energy and node velocity are used to calculate the node stability factor. The proposed algorithm is simulated by using NS-2 simulation, and is compared with other multicast routing mechanisms: ODMRP and PUMA. Packet delivery ratio, multicast route lifetime, and control packet overhead are used as performance metrics. These metrics are measured by gradual increase of the node mobility, the number of sources, the group size and the number of groups. The simulation performance results indicate that the proposed algorithm outperforms other mechanisms in terms of routes stability and network density.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.317.2-317.2
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• 2013

Ultrathin oxide encapsulated metal-oxide hybrid nanocatalysts have been fabricated by a soft chemical and facile route. First, SiO2 nanoparticles of 25~30 nm size have been synthesized by modified Stobber's method followed by amine functionalization. Metal nanoparticles (Ru, Rh, Pt) capped with polymer/citrate have been deposited on functionalized SiO2 and finally an ultrathin layer of TiO2 coated on surface which prevents sintering and provides high thermal stability while maximizing the metal-oxide interface for higher catalytic activity. TEM studies confirmed that 2.5 nm sized metal nanoparticles are well dispersed and distributed throughout the surface of 25 nm SiO2 nanoparticles with a 3-4 nm TiO2 ultrathin layer. The metal nanoparticles are still well exposed to outer surface, being enabled for surface characterization and catalytic activity. Even after calcination at $600^{\circ}C$, the structure and morphology of hybrid nanocatalysts remain intact confirm the high thermal stability. XPS spectra of hybrid nanocatalyst suggest the metallic states as well as their corresponding oxide states. The catalytic activity has been evaluated for high temperature CO oxidation reaction as well as photocatalytic H2 generation under solar simulation. The design of hybrid structure, high thermal stability, and better exposure of metal active sites are the key parameters for the high catalytic activity. The maximization of metal-TiO2 interface interaction has the great role in photocatalytic H2 production.

• Proceedings of the Korean Geotechical Society Conference
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• 2005.10a
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• pp.601-612
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• 2005

In mountainous area, Two parallel tunnels have been usually recognized as a road tunnel which has benefits in aspects of cost and stability. However, Design and construction of 2-Arch road tunnel are growing recently due to environmental destruction, compensation of land and difficulty of route separation. As studies are mainly undergoing on only guaranteeing stability and developing a waterproofing-drainage system to avoid water leakage through comprehension for characteristics of 2-arch tunnel behaviors, there is a tendency to evaluate quantity of support by empirical method with a tunnel which has a complicated cross-section and lack of construction ability. In this study, therefore, we made a plan of tunnel cross-section which had shown good construction ability and developed the waterproofing-drainage system which is able to solve the water leakage problem fundamentally by analyzing precedented 2-arch tunnels and investigating their sites in and out of nation. We also determined fixed quantity of support by a large-scale model test and numerical analysis. We want to contribute to 2-arch tunnel design hereafter introducing design procedure and method applied here.

• Convergence Security Journal
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• v.14 no.7
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• pp.37-43
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• 2014

Today, group research efforts are increasing for cutting edge technologies related to climate, radio astronomy, co-utilization of high performance computing resources and more. Accordingly, networks for cutting-edge researches a re also regarded as means for cutting-edge researches nowadays. Therefore, it is necessary for administrating cutti ng-edge network to be considered, reflecting such a current trend. In this sense, 'Research which can help to admin istrate the networks which are usually used as means for a lot researches' was set as a goal of this paper.