• 제목/요약/키워드: Rotary system

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Integrated Rotary Genetic Analysis Microsystem for Influenza A Virus Detection

  • Jung, Jae Hwan;Park, Byung Hyun;Choi, Seok Jin;Seo, Tae Seok
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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    • pp.88-89
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    • 2013
  • A variety of influenza A viruses from animal hosts are continuously prevalent throughout the world which cause human epidemics resulting millions of human infections and enormous industrial and economic damages. Thus, early diagnosis of such pathogen is of paramount importance for biomedical examination and public healthcare screening. To approach this issue, here we propose a fully integrated Rotary genetic analysis system, called Rotary Genetic Analyzer, for on-site detection of influenza A viruses with high speed. The Rotary Genetic Analyzer is made up of four parts including a disposable microchip, a servo motor for precise and high rate spinning of the chip, thermal blocks for temperature control, and a miniaturized optical fluorescence detector as shown Fig. 1. A thermal block made from duralumin is integrated with a film heater at the bottom and a resistance temperature detector (RTD) in the middle. For the efficient performance of RT-PCR, three thermal blocks are placed on the Rotary stage and the temperature of each block is corresponded to the thermal cycling, namely $95^{\circ}C$ (denature), $58^{\circ}C$ (annealing), and $72^{\circ}C$ (extension). Rotary RT-PCR was performed to amplify the target gene which was monitored by an optical fluorescent detector above the extension block. A disposable microdevice (10 cm diameter) consists of a solid-phase extraction based sample pretreatment unit, bead chamber, and 4 ${\mu}L$ of the PCR chamber as shown Fig. 2. The microchip is fabricated using a patterned polycarbonate (PC) sheet with 1 mm thickness and a PC film with 130 ${\mu}m$ thickness, which layers are thermally bonded at $138^{\circ}C$ using acetone vapour. Silicatreated microglass beads with 150~212 ${\mu}L$ diameter are introduced into the sample pretreatment chambers and held in place by weir structure for construction of solid-phase extraction system. Fig. 3 shows strobed images of sequential loading of three samples. Three samples were loaded into the reservoir simultaneously (Fig. 3A), then the influenza A H3N2 viral RNA sample was loaded at 5000 RPM for 10 sec (Fig. 3B). Washing buffer was followed at 5000 RPM for 5 min (Fig. 3C), and angular frequency was decreased to 100 RPM for siphon priming of PCR cocktail to the channel as shown in Figure 3D. Finally the PCR cocktail was loaded to the bead chamber at 2000 RPM for 10 sec, and then RPM was increased up to 5000 RPM for 1 min to obtain the as much as PCR cocktail containing the RNA template (Fig. 3E). In this system, the wastes from RNA samples and washing buffer were transported to the waste chamber, which is fully filled to the chamber with precise optimization. Then, the PCR cocktail was able to transport to the PCR chamber. Fig. 3F shows the final image of the sample pretreatment. PCR cocktail containing RNA template is successfully isolated from waste. To detect the influenza A H3N2 virus, the purified RNA with PCR cocktail in the PCR chamber was amplified by using performed the RNA capture on the proposed microdevice. The fluorescence images were described in Figure 4A at the 0, 40 cycles. The fluorescence signal (40 cycle) was drastically increased confirming the influenza A H3N2 virus. The real-time profiles were successfully obtained using the optical fluorescence detector as shown in Figure 4B. The Rotary PCR and off-chip PCR were compared with same amount of influenza A H3N2 virus. The Ct value of Rotary PCR was smaller than the off-chip PCR without contamination. The whole process of the sample pretreatment and RT-PCR could be accomplished in 30 min on the fully integrated Rotary Genetic Analyzer system. We have demonstrated a fully integrated and portable Rotary Genetic Analyzer for detection of the gene expression of influenza A virus, which has 'Sample-in-answer-out' capability including sample pretreatment, rotary amplification, and optical detection. Target gene amplification was real-time monitored using the integrated Rotary Genetic Analyzer system.

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SEA에 의한 회전 압축기의 소음 진동 해석 (Noise and Vibration Analysis of Rotary Compressor by SEA)

  • 황선웅;안병하;정의봉;김규환
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 2003년도 춘계학술대회논문집
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    • pp.964-968
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    • 2003
  • Hermetic rotary compressor is one of the most Important components for air conditioning system since it has a great effect on both the performance and the noise and vibration of He system. Noise and vibration of rotary compressor is occurred due to gas pulsation during compression process and unbalanced dynamic force. In order to reduce noise and vibration. it is necessary to identify sources of noise and vibration and effectively control then. Many approaches have been tried to identify noise sources of compressor. However, compressor noise source identification has proven to be difficult since the characteristics of compressor noise are complicated due to the interaction of the compressor parts and gas pulsation. In this work, Statistical Energy Analysis has been used to trace the energy flow in the compressor and identify transmission paths from the noise source to the sound field.

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트윈 로타리 압축기의 토출 가스 맥동 해석 (Analytical Study on the Discharge Gas Pulsation in a Twin Rotary Compressor)

  • 김현진;안종민;조광명
    • 유체기계공업학회:학술대회논문집
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    • 유체기계공업학회 2003년도 유체기계 연구개발 발표회 논문집
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    • pp.697-703
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    • 2003
  • For a single stage two cylinder rotary compressor, an analytical study has been made on the discharge gas pulsation. Discharge system of the twin rotary compressor consists of lower and upper mufflers and connecting passage holes between them, and cavities on both sides of the motor and passages between them. Acoustic modeling for the discharge system by transfer matrix method gives acoustic impedances at discharge valves so that gas pulsation at the valve sections can be obtained from discharge mass velocity. Since the mass velocity and the pressure pulsation at the valves are affected by each other, iteration should be made for convergence. Gas pulsations at other sections can also be calculated by using transfer matrix.

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분말소재의 표면처리를 위한 회전형 CVD 공정 (Rotary CVD Process for Surface Treatment of Powders)

  • 이종환;정구환
    • 한국표면공학회지
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    • 제56권6호
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    • pp.341-352
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    • 2023
  • This paper reviews the potentials of a rotary chemical vapor deposition (RCVD) process for nanomaterial synthesis and coating on powder-based materials. The rotary reactor offers a significant improvement over traditional CVD methods having horizontal and fixed reaction chambers. The RCVD system yields enhanced productivity and surface coating uniformity of nanoparticles applied in various purposes, such as efficient heat dissipation, surface hardness enhancement, and enhanced energy storage performances. The effectiveness of the RCVD system would open up new possibilities in various applications because uniform coating on powder-based materials with massive productivity is inevitable to develop multi-functional materials with high reliability.

Embeded system을 적용한 Rotary Inverted Pendulum 설계 및 PID에 의한 구현 (Design of Rotary Inverted Pendulum applying an Embedded System and Implementation by PID)

  • 김영춘;김정훈;김영탁;김동한
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2002년도 하계종합학술대회 논문집(5)
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    • pp.5-8
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    • 2002
  • In this paper, we applied a PC interface and an embedded system in order to design a non-linear system and implement the PID algorithm as our control one. We used the inverted pendulum, one of the most generally used non-linear system models, to control uncertain factors in the environment. This paper showed how to use this non-linear system model to control the factors completely as well as to understand the PID algorithm. Furthermore, this paper applied and understood the embedded system.

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웹기반 동적 설계 협업 지원 시스템 개발 (Development of a Web-based Vibration Analysis System for a Rotary Compressor)

  • 한형석;이재경
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 2003년도 춘계학술대회 논문집
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    • pp.1739-1742
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    • 2003
  • This paper introduces a Web-based vibration analysis system for rotary-type refrigerator compressors. Concern for vibration reduction in compressors has been growing for many years. What is important in vibration reduction is to easily predict the vibration without using a physical compressor. The dynamic model of the compressor is represented as a multi-body dynamic system. Solving the dynamic model is run on a high-performance server. The interfaces of the system are accessible via Web browsers, such as Netscape or Explorer. Anyone who wants to analyze the vibration of the rotary compressor or share the results data can access the system over the Internet regardless of their OS, platform. or location.

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광탄성법(光彈性法)에 의한 로터리 경운날의 파괴요인(破壞要因)에 대한 해석(解析) -정하중(靜荷重)에 의한 응력집중(應力集中)- (Analysis of the Breaking Factor of Rotary Blade by Photo elastic Method -A Stress Concentration by Static Load-)

  • 최상인;김진현;김창수;김재열
    • Journal of Biosystems Engineering
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    • 제15권3호
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    • pp.177-185
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    • 1990
  • The break of rotary blade is occured from a stress concentration of the inside of blade by the outside impulsive load. In order to examine its inside stress and stress concentration of rotary blade, a epoxy plate which is suitable to applicate by photoelastic system is used to experiment. These results are summarized as follow. 1. Refer to the existence of bolt hole and a size of its of rotary blade, a stress concentration which cause the break of rotary blade is not exposed. 2. It is expected to be break to section of hold of rotary blade and the break of this is due to that there are concentrated by shearing force, bending moment and bending stress. 3. When the crack which caused from processing are set up to any location, the stress concentration taken to the creak point. 4. Without regard to the location of the reaction points of rotary blade, the bending stress which is greated than the bending moment is occured within about 6 em toward the center line of bolt hole and it was possible to break that section.

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Continuous rotary system과 single file system의 만곡 근관 형태 유지능에 대한 비교 연구 (Comparative Study on the Ability of Instruments to Maintain Original Canal Curvature of Continuous rotary System and Single File System)

  • 박상희;김덕중;송용범;이혜윤;김형선;이광원;유미경
    • 구강회복응용과학지
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    • 제28권4호
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    • pp.371-383
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    • 2012
  • 성공적인 근관치료를 위하여 원래 근관 형태를 따르는 근관 성형 과정이 중요하다. 그러나 대부분의 근관은 만곡되어 있고, 근관 성형 기구는 직선의 금속을 기본으로 만들어지기 때문에 근관 성형 후 만곡 근관이 원래 근관 형태를 벗어나는 오류가 나타날 수 있다. 이런 오류들로 인하여 근관 치료가 실패할 수 있으므로 근관 성형시 원래 만곡형태를 유지할 수 있는 다양한 기술과 방법들이 소개되고 있다. 본 연구의 목적은 만곡 근관 형태의 레진 모형을 이용하여 기구의 형태와 사용방법이 다른 세 가지 Ni-Ti 파일을 사용하여 Ni-Ti 파일 종류에 따른 만곡 근관에 대한 성형 후 형태 유지능을 비교하는 것이다. 직경과 경사도가 ISO 15, 0.02인 30개의 레진모형을 사용하였다. 그룹 1은 Profile을 이용하여 #20/.06까지 근관 성형하였고, 그룹 2는 Mtwo를 이용하여 #35/.04까지 근관 성형하였으며, 그룹 3은 PathFile을 이용하여 glide path를 만들어준 다음 WaveOne 파일 중 Primary을 사용하여 근관 성형하였다. 기구 사용 전과 후의 레진모형을 스캔하여 디지털 이미지로 만들었다. 각 이미지를 중첩시킨 다음 Matlab r2010b 소프트웨어를 이용하여 곡률반경의 비(CRr)를 계산하였다. 이를 바탕으로 Ni-Ti file 간 성형 후 형태 유지능을 비교하기 위하여 1-way ANOVA를 이용하여 세 그룹의 CRr의 차이에 대한 유의성 검정을 시행하였다.(P<.05) 또한 continuous rotary system과 single file system간의 형태 유지능을 비교하기 위하여 independent t-test를 이용하였다.(P<.05) 곡률반경의 비(CRr)를 이용하여 Ni-Ti 파일 종류에 따른 만곡 근관의 형태 유지능을 비교한 결과 Ni-Ti file 간에는 통계적으로 유의한 차이가 있었다.(P<0.05) ProFile과 WaveOne은 Mtwo에 비해서 만곡 근관에서의 형태 유지능이 우수하게 나타났다.Ni-Ti 기구 사용 방법에 따른 만곡 근관 형태 유지능을 비교한 결과 continuous rotary system과 single file system 간에는 통계적으로 유의한 차이를 보이지 않았다.(P>0.05) 본 연구결과에 따르면, ProFile과 WaveOne은 Mtwo에 비하여 만곡 근관에서의 형태 유지능이 통계적으로 유의하게 높게 나타났으므로, ProFile과 WaveOne이 만곡근관에 대한 형태 유지능이 우수하다고 할 수 있다. 만곡근관에서의 형태 유지능에 있어서 continuous rotary system과 single file system은 만곡근관에서의 형태 유지능에 차이가 없다.

차분진화 알고리즘을 이용한 회전형 역 진자 시스템의 최적 퍼지 제어기 설계 (Design of Optimized Fuzzy Controller for Rotary Inverted Pendulum System Using Differential Evolution)

  • 김현기;이동진;오성권
    • 전기학회논문지
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    • 제60권2호
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    • pp.407-415
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    • 2011
  • In this study, we propose the design of optimized fuzzy controller for the rotary inverted pendulum system by using differential evolution algorithm. The structure of the differential evolution algorithm has a simple structure and its convergence to optimal values is superb in comparison to other optimization algorithms. Also the differential evolution algorithm is easier to use because it have simpler mathematical operators and have much less computational time when compared with other optimization algorithms. The rotary inverted pendulum system is nonlinear and has a unstable motion. The objective is to control the position of the rotating arm and to make the pendulum to maintain the unstable equilibrium point at vertical position. The output performance of the proposed fuzzy controller is considered from the viewpoint of performance criteria such as overshoot, steady-state error, and settling time through simulation and practical experiment. From the result of both simulation and practical experiment, we evaluate and analyze the performance of the proposed optimal fuzzy controller from the comparison between PGAs and differential evolution algorithms. Also we show the superiority of the output performance as well as the characteristic of differential evolution algorithm.