• Archives of Pharmacal Research
• /
• v.25 no.6
• /
• pp.860-864
• /
• 2002

In this study the composition and antimicrobial properties of essential oils obtained from Origanum onites, Mentha piperita, Juniperus exalsa, Chrysanthemum indicum, Lavandula hybrida, Rosa damascena, Echinophora tenuifolia, Foeniculum vulgare were examined. To evaluate the in vitro antibacterial activities of these eight aromatic extracts; their in vitro antimicrobial activities were determined by disk diffusion testing, according to the NCCLS criteria. Escherichia coli (ATTC 25922), Staphylococcus aureus (ATCC 25923) and Pseudomonas aeruginosa (ATTC 27853 were used as standard test bacterial strains. Origanum onites recorded antimicrobial activity against all test bacteria, and was strongest against Staphylococcus aureus. For Rosa damascena, Mentha piperita and Lavandula hybrida antimicrobial activity was recorded only to Staphylococcus aureus. Juniperus exalsa, and Chrysanthemum indicum exhibited antibacterial activities against both Staphylococcus aureus and Escherichia coli. We also examined the in vitro artimicrobial activities of some components of the essential oils and found some components with antimicrobial activity.

• Horticultural Science & Technology
• /
• v.17 no.4
• /
• pp.481-484
• /
• 1999

This experiment was conducted to examine the effects of chitosan and sucrose on the vase life of cut rose (Rosa hybrida L. cv. Cardinal). Addition of 5 mg/L chitosan and 3% sucrose to the holding solution prolonged the vase life for 3 days as compared to the control, and improved quality of cut rose in flower diameter and fresh weight. Development of bent-neck was delayed by supply of 2 mg/L or 5 mg/L chitosan and 3% sucrose about 2 or 3 days. When 3% sucrose was added to the holding solution, the rate of water absorption more slowly decreased compared to the other treatments.

• Journal of Applied Biological Chemistry
• /
• v.49 no.4
• /
• pp.180-185
• /
• 2006

Two different extraction procedures, SDE and SPME, were employed to determine a comprehensive and efficient fragrance profile of Korean Rosa hybrida. Both extraction methods could compensate for each other, covering compounds with diverse boiling point, polarities, and chemical properties. A total of 46 compounds were identified in Mi-hyang. The identified compounds were composed of 17 alcohols, 14 carbonyls, 7 aliphatic hydrocarbons, 2 terpene hydrocarbons, 4 benzenes, 1 ester, and 1 miscellaneous compound. Quantitatively, carbonyls($12.96{\sim}21.79%$ in essential oils of SDE and $2.89{\sim}8.44%$ in SPME headspace) and alcohols($7.98{\sim}11.73%$ in essential oils of SDE and $3.39{\sim}17.35%$ in SPME headspace) were dominant in Mi-hyang's volatiles.

• Plant Biotechnology Reports
• /
• v.3 no.4
• /
• pp.341-345
• /
• 2009

This study describes culture conditions for a plant regeneration system via a combined pathway of somatic embryogenesis and organogenesis in root explant cultures of the commercial rose cultivar 'Charming'. Root explants formed white calluses at a frequency of 30% after 6 weeks of culture on Schenk and Hildebrandt (SH) medium supplemented with $11mg\;1^{-1}$ 2,4-dichlorophenoxyacetic acid. After 6 weeks of transfer to SH medium without growth regulators, initial white calluses gave rise to globular somatic embryos at a frequency of 2.8%, which were subsequently dedifferentiated to embryonic tissues. Somatic embryos or embryonic tissues initially derived from root explants did not undergo development beyond cotyledonary stage. To produce adventitious shoots, embryonic tissues were sliced and cultured on SH medium with $0.5mg\;1^{-1}$ 6-benzyladenine. After 4 weeks of culture, 28% of embryonic tissue explants formed adventitious shoots. Regenerated shoots were rooted on half strength SH medium with $0.1mg\;1^{-1}$ ${\alpha}-naphthalaneacetic$ acid and subsequently grown to maturity. Root-derived embryonic tissues were proliferated by subculture, while retaining the capacity for shoot production for a few years.

• FLOWER RESEARCH JOURNAL
• /
• v.18 no.2
• /
• pp.147-150
• /
• 2010

A new cultivar potted miniature rose (Rosa hybrida) 'Tiny Ball' was selected out of cross combination 1999 and 2002 at Gumi Floricultural Experiment Station. Characteristics trials were conducted three times from 2003 to 2006. 'Tiny Ball' came from the combination that '990905' crossed to 'Manish'. New cultivar has pink and white flower color and the shape of flower is similar to tiny ball. The number of flower petals and flowers per pot are more than those of 'POULra022' as comparison cultivar. This cultivar has low plant height without treatment of plant retardant materials, so the high quality grade ratio of potted rose is higher than that of comparison cultivar.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.31 no.3
• /
• pp.539-542
• /
• 2002

To evaluate a flower of rose, Rosa hybrids L. as a new food material, its chemical composition was analysed. The contents of crude protein, crude fat, crude ash and crude fiber in rose petals were 16.3, 2.9, 5.4, 16.1% on dry basis, respectively. Free sugars showed 74.3 mg/g of fructose, 49.6 mg/g of glucose and 16.6 mg/g of xylose. The contents of ${\beta}$-carotene and ascorbic acid were 205.2 ug/100 g and 129.5 mg/100 g, respectively. The major minerals of rose petals were K, P, Mg, Ca, Na and Fe, and among them K was the most abundant as 1,981.7 mg/100 g. The major amino acids were aspartic acid as 4,007.3 mg/100 g, glutamic acid as 1,114.8 mg/100 g, lysine as 672.6 mg/100 g and leucine as 661.0 mg/100 g. Fatty acids were mainly unsaturated fatty acids as 76.3%.

• Journal of Bio-Environment Control
• /
• v.16 no.2
• /
• pp.115-120
• /
• 2007

An experiment was conducted to measure rooting and growth of cuttings of rose as affected by cutting length, number of leaflets and cutting position. Test plants were two cultivars of Rosa hybrida 'Red Sandra'(standard) and 'Little Marble'(miniature). Cutting length was 4, 7 or 10cm for 'Red Sandra' and 3, 5 or 7 cm for 'Little Marble'. The number of leaflets left on the five-leaflet leaf was 0, 2, 4 or 5. The cutting position on the stem was between $1{\sim}2,\;3{\sim}4,\;5{\sim}6\;or\;7{\sim}8$ nodes from the shoot tip, which have five leaflet leaves. The most efficient cutting length was 7cm in both cultivars. Treatment with all leaflets left and cutting position at $1{\sim}2$ nodes resulted in good rooting ratio for both cultivars.

• Journal of Bio-Environment Control
• /
• v.16 no.4
• /
• pp.309-313
• /
• 2007

Experiments were conducted to measure rooting and growth of rose cuttings as affected by medium composition and volume. Rosa hybrida 'Red Sandra' and 'Little Marble' were used as test plants. For medium composition test, rockwool, phenolic foam, perlite, vermiculite, peatmoss, coir and a commercial medium (Tosilee medium) were applied in single or mixed forms on the propagation bed with fog (200 sec./5 min.) installed to control the humidification time. 'Red Sandra' and 'Little Marble' cuttings were planted in 16-cell plastic pots and 72-cell plug trays, respectively. For the medium volume test, 72-cell plug trays and 16-cell or 9-cell plastic pots were used. Cuttings with an uniform node with a five-leaflet leaf was soaked in a $500mg{\cdot}L^{-1}$ IBA solution for 30 sec. A fogging system installed on the propagation bed was used for (100 sec./5 min.). Rockwool 1 : peatmoss 1 (v/v) was the most effective on growth and rooting for both cultivars and high volume of Tosilee medium and rockwool were most effective media far both cultivars.

• Horticultural Science & Technology
• /
• v.28 no.5
• /
• pp.796-801
• /
• 2010

This study was carried out to establish a system for mutation breeding by irradiation of gamma-ray in $Rosa$ $hybrida$ Hort. The rooted cuttings of two roses, 'Spidella' and 'Cabernet' were irradiated with different gamma-ray doses (0, 30, 50, 70, 90, 110, 130, 150 and 170 Gy) from a $^{60}Co$ source to reveal an optimal dose for induction of mutants. The irradiated plants were planted in a greenhouse, and investigated on the appearance of petal color mutants and shoot growth by gamma ray dose. The 50% lethal doses ($LD_{50}$) of plant were 110 Gy for 'Spidella' and 150 Gy for 'Cabernet', respectively. The 50% decrease dose of shoot length was observed at 70-90 Gy dose for 'Spidella', and 110 Gy dose for 'Cabernet'. Solid, chimeric and mosaic petal mutants with various colors were induced from pink petal of 'Spidella' and red petal of 'Cabernet' when 30-170 Gy dose was irradiated. The mutants obtained from 'Spidella' had white, ivory, pinky ivory, light pink and deep pink petal colors. The mutants obtained from 'Cabernet' had pink, deep pink, purple red (magenta), orange red and purple petal colors. It was suitable to irradiate 70-90 Gy dose for 'Spidella' and 90-110 Gy dose for 'Cabernet' for the induction of various mutants considering plant survival rate, shoot growth and mutant occurrence rate.

• Plant Biotechnology Reports
• /
• v.3 no.3
• /
• pp.199-203
• /
• 2009

Rugosa rose (Rosa rugosa) is cultivated as a garden flower and an important genetic resource for the breeding of roses (R. hybrida). This study describes culture conditions for high frequency plant regeneration from zygotic embryo explants via somatic embryogenesis in rugosa rose. Mature zygotic embryo, cotyledon, and radicle explants formed embryogenic calluses at frequencies of 38, 6.7, and 8.8% when cultured on half-strength Murashige and Skoog medium (${\frac{1}{2}}MS$) supplemented with 2.26, 9.05, and $9.05{\mu}M$ 2,4-dichlorophenoxyacetic acid, respectively. Embryogenic calluses produced numerous somatic embryos, which then developed into plantlets on ${\frac{1}{2}}MS$ without growth regulators. Regenerated plantlets were grown to whole plants in a growth chamber.