• Research in Plant Disease
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• v.19 no.2
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• pp.108-113
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• 2013

Wilt damage on tomato plants caused by Ralstonia solanacearum has been increased as the areas of tomato cultivation increased during the warm seasons. Also, the tomato rootstocks used to prevent the disease occurrence are not effective in the highly prevailing regions. Therefore, bacterial wilt resistant eggplant rootstock EG203, collected from AVRDC, was tested for its effect to deter the Ralstonia solanacearum wilt disease in the greenhouses at Buyeo Tomato Experiment Station from 2003 to 2005, and at Gumi, Kyungpook province from 2009-2011. Planting of eggplant rootstock EG203 should be done three weeks before the planting of tomato scions so that they can have similar stem diameter (2.5-3.0 mm) and can be easily grafted. Both insertion and inarching grafting showed 93-96% success rates. In the greenhouse tests at Buyeo Tomato Experiment Station from 2003 to 2005, eggplant rootstock EG203-grafted tomatoes showed the disease occurrence of 4.3%. On the other hand, non-grafted or other commercial rootstock-grafted tomatoes showed disease occurrence of 58.0% and 25.0-36.7%, respectively. In the greenhouse tests at Gumi, Kyungpook province in 2009, the disease occurrence on the EG203-grafted and non-grafted tomatoes was 2-5% and 20-80%, respectively. In 2010, at Gumi, Kyungpook province, when the wilt disease occurred slightly, the tomatoes grafted with tomato rootstocks B-blocking and Chung-gang, and eggplant rootstock EG203 showed similar disease severities, but EG203-grafted tomatoes formed lately cluster, resulting in the reduction of yield compared to tomato-grafted tomatoes. In 2011, at Gumi, Kyungpook province, when the wilt disease occurred severely, the tomato rootstocks 'B-blocking' and Chung-gang and eggplant rootstock EG203-grafted tomatoes showed disease occurrences of 60-85% and 0-1%, respectively. Therefore, it was concluded that tomato rootstocks 'B-blocking' and 'Chung-gang' are more useful in the areas contaminated with low levels of pathogen and eggplant rootstock EG203 is more useful in the areas contaminated with high levels of pathogen.

• Journal of Bio-Environment Control
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• v.15 no.3
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• pp.283-288
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• 2006

Characteristics of the rootstock formation and flower bud differentiation according to sowing time for production of the high quality rootstock in Chicory (Cichorium intybus L.) was investigated. The flower bud initiation was on the 17th of July after sowing in April, the 2nd of July after sowing in May, the 30th of July after sowing in June and the 1st of October after sowing in July. But they didn't differentiate after sowing in August and September. The days required from germination to flower bud initiation and accumulated temperature depended on the weather. If the outside air temperature was high, the days required were less, If the outside air temperature was low, the days required were greater, the accumulated temperature was similar to the days required in this experement, too. The rootstock sown from April to July wasn't good enough to be use because the flower bud differentiation was generated before harvestable weight. the weight of rootstock was sown in September wasn't heavy enough in weight because the outside air temperature was too low for it to grow well. The plant grows slow and steady through out winter. The rootstock sown in August was havestable 200g in 90 days after sowing before the flower bud differentiated. The best sowing time for the production of high quality rootstock of Chico in the climate of Korea is researched to be August.

• Journal of agriculture & life science
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• v.50 no.5
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• pp.81-93
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• 2016

Rehmannia glutinosa(Gaertn.) DC. is a herbaceous perennial plant and belonging to the Scrophulariaceae and used as roots for medicinal part and purpose. R. glutinosa is and usually used for fresh rehmannia root or prepared rehmannia root. However, it is very difficult to propagate using the seeds because of lack germination so it is propagated using the vegetative method as the rootstock. Currently, propagation and harvesting using the rootstock of R. glutinosa has difficulties about production of the high quality and quantity in R. glutinosa because of root rot disease. To optimize in vitro cultures and to improve the rootstock and seedling of R. glutinosa after morphological and genetical determination, 5 plant culture media (MS, DJ, LS, QL, and WPM) were used in this study then WPM was selected for better growth, for multiplication condition(WPM + IAA 1.0 mg/L + IBA 0.5 mg/L), and for root enlargement condition(WPM + NAA 0.1 mg/L) of R. glutinosa. Based on these results, in vitro seedlings of R. glutinosa were transferred to soil for acclimation with environment adaptation and shown the positive effects about root enlargement and root formation. Therefore, it can be used for high quality of R. glutinosa production and production of the rootstock based on propagation using in vitro seedlings of R. glutinosa.

• Current Research on Agriculture and Life Sciences
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• v.15
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• pp.115-122
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• 1997

Grafting is an important skill for the stable supply and production of high quality. However, the shortage of skillful labor has become great difficulty for a mass production of grafting-seedling. In this study, a suitable mechanism for a grafting machine was developed. The following summarize the results of this study: 1. An insertion method was selected for mechanism of the grafting machine without bonding agent, clip, pin. This insertion-grafting method can be applicable to general vegetables and a mass production system. In addition to, this method is suitable for developing the grafting mechanism. 2. Growing point was removed while remaining both cotyledons on rootstock. The productivity of this system was five fold greater than the one of an experienced labor. 3. The rootstock processing was placed on left and scion processing unit was placed on right of the system, then processed rootstock and scion graft by rotating $180^{\circ}$. 4. The efficiency tests on mechanical grafting rate showed 98%.

• Journal of Bio-Environment Control
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• v.15 no.4
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• pp.417-420
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• 2006

To investigated the dormancy of chicory (Cichorium intybus L.) for production of high quality rootstocks, growth characteristics and abscisic acid (ABA) contents was conducted. The accumulation hours below $5^{\circ}C$ in field was increased slowly from October 7 to December 3, but there after increased largely. The rootstock weights was increased from the end of October to December 3, there after was not changed to December 3. ABA contents within the rootstock was not changed from October 7 to December 3, and there was suddenly increased for two weeks to December 24. As results of the sudden changed date of accumulation hours below $5^{\circ}C$, stopped date and period of rootstock growth, and sudden changed period of ABA contents, chicory has internal dormancy for two weeks from December 3 and low temperature requirement hours for the dormancy breaking is about 240.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.687-699
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• 2013

This study was conducted to examine the effect of grafted peppers (Capsicum annuum) on different rootstocks on fruit quality. Three pepper cultivars, 'Nokkwang', 'Saengsaeng Matkkwari', and 'Shinhong' were grafted onto five commercial rootstocks that are known to be resistant to Phytophtora blight. Non-grafted or auto-grafted peppers were used as controls. Grafted plants were grown during two consecutive harvest periods by semi-forcing culture (April to August) and retarding culture (September to March the subsequent year). Full size green fruits were harvested and weighed weekly from June to August (Semi-forcing culture) and from December to March of the subsequent year (Retarding culture). The fruit size, weight, flesh thickness, and firmness were measured every month. Total marketable yield was not significantly influenced by either auto-graft of 'Nokkwang', 'Saengsaeng Matkkwari', and 'Shinhong' of pepper or grafted with the five commercial rootsctocks. By contrast, grafting influenced the apparent fruit quality of peppers. Fruit characteristics differed depending on the rootstock cultivars. However, the fruit characteristics of rootstock did not affect the fruit characteristics of scion grafted onto that rootstock. Fruit characteristics in each treatment differed among harvest time (first, second, and third harvest). Fruit quality parameters were also different as affected by the harvest period. In conclusion, apparent quality and textural property of pepper fruits were influenced by not only grafting with different rootstocks but also by the harvest period and harvest time. Accordingly, rootstock/scion combination, the scion variety and the harvest period must be carefully chosen to get the desired optimal fruit quality.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.5
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• pp.449-455
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• 2016

'Gamhong' apple cultivar which was bred by National Institute of Horticultural and Herbal Science, has high sugar contents, large types of fruit and mid-season types of cultivar. However, 'Gamhong' apple tree is very sensitive cultivar to bitter pit. Therefore, many farms have not solved the problems on bitter pit to cultivate 'Gamhong' apple tree. This study was carried out to find out the exposed length of rootstocks on the occurrence of bitter pit and fruit quality of 'Gamhong'/M.26 apple tree and seek the ideal cultivation strategy to decrease bitter pit to cultivate it. For this research, ten-years-old 'Gamhong'/M.26 apple trees were used. The difference among the treatments of the exposure of rootstock of RL (5 cm), RM (15 cm) and RH (20 cm) from the soil surface was observed with 4 repetition from 2010 to 2011. Decreased exposure of rootstocks resulted in more vigorous growth, taller height, and greater number of shoots. N and K/Ca of RH was tend to be lower than RM and RL, whereas as the exposure of rootstock decreased, fruit weight and bitter pit increased. Therefore, occurrence of bitter pit in RH was significantly lower than that of RM and RL treatment and fruit weight also decreased. There was no difference on nutrient contents of leaf and the fruit quality by the exposed of rootstock in 'Gamhong' M.26 apple tree. To reduce the occurrence of bitter pit, it may be helpful to keep the optimum exposure of rootstock within 20 cm from the soil surface.

• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.78-84
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• 2005

Charactertistics of growth from graft induced three stock of red pepper cultivar were analysed. R-safe rootstock was more higher and vigorous than that of the Yeok kang, Konesian hot cultivar at seedling stage and had good characteristics for grafting in the space of cut surface and the amount of sap released. Numbers of branches were more numerous in the grafted plants than those of non-grafted as grafting affected their growths in the process of branching. There was no distinct difference in plant height among the different rootstock. However the R-safe rootstock showed considerably high growth in the 41st days after grafting. Grafting was effective in the early flowering and the R-safe was the earliest in flowering because of it's good growth under the low temperature.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.48-48
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• 2020

Fire blight, caused by Erwinia amylovora(Burrill), is a destructive disease of apple that damages blossoms, shoots, and woody plant organs. The fire blight disease is a worldwide problem for pome fruit growers because all popular apple cultivars are susceptible to the disease. Recently, fire blight of apple rootstocks has become a serious economic problem in high-density orchard systems in korea. The most commonly used dwarfing root stocks, M.9 and M.26, are highly susceptible to E. amylovora. The objective of the apple rootstock-breeding program has been to develop pomologically excellent rootstocks with resistance to abiotic and biotic stresses, including fire blight. Budagovsky 9 (B.9) apple rootstock is reported to be highly susceptible when inoculated with E. amylovora, although results from multiple trials showed that B.9 is resistant to rootstock blight infection in field plantings. So we tried to collect the apple rootstocks traits of fire blight resistance. The apple genotype Malus Robusta 5 (MR5) represents an ideal donor for fire blight resistance because it was described as resistant to all currently known European strains of the pathogen. The PCR for detecting the MR5 gene using the primers Md_MR5_FL_F/Md_MR5_FL_R. The results of these experiments confirmed some apple rootstocks traits of fire blight resistance showed the MR5. Furthermore, this gene is confirmed to be the resistance determinant of Mr5 as the transformed lines undergo the same gene-for-gene interaction in the host-pathogen relationship MR5-E. amylovora.

• Plant Biotechnology Reports
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• v.4 no.2
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• pp.101-107
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• 2010

An efficient plant regeneration system has been developed for figleaf gourd (Cucurbita ficifolia Bouch$\'{e}$), which is exclusively used as a rootstock for cucumber. The protocol is based on results obtained from a series of culture experiments involving different parts of the cotyledons and various media. The culture of cotyledon explants was critical for the enhancement of shoot regeneration frequency. The lower parts of the cotyledon excised at the plumule base were found to display a markedly enhanced production of adventitious shoots compared to other cotyledon regions. Culture in silver nitrate-supplemented Murashige and Skoog (MS) medium was not beneficial for shoot regeneration and suppressed root regeneration. Efficient shoot regeneration was obtained on MS medium containing 1.0 $mg\;l^{-1}$ zeatin and 0.1 $mg\;l^{-1}$ indole-3-acetic acid. Regenerated shoots successfully elongated and rooted in medium containing 0.1 $mg\;l^{-1}$ 1-naphthalene-acetic acid after 10-15 days of subculturing. The plantlets were satisfactorily acclimatized in a greenhouse and grew into normal plants without any morphological alterations.