• Korean Journal of Radiology
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• 제22권2호
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• pp.225-232
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• 2021

Objective: To determine whether changes in the transiting nerve rootlet or its surroundings, as seen on MRI performed after lumbar hemilaminectomy, are associated with persistent postoperative pain (PPP), commonly known as the failed back surgery syndrome. Materials and Methods: Seventy-three patients (mean age, 61 years; 43 males and 30 females) who underwent single-level partial hemilaminectomy of the lumbar spine without postoperative complications or other level spinal abnormalities between January 2010 and December 2018 were enrolled. Two musculoskeletal radiologists evaluated transiting nerve rootlet abnormalities (thickening, signal alteration, distinction, and displacement), epidural fibrosis, and intrathecal arachnoiditis on MRI obtained one year after the operations. A spine surgeon blinded to the radiologic findings evaluated each patient for PPP. Univariable and multivariable analyses were used to evaluate the association between the MRI findings and PPP. Results: The presence of transiting nerve rootlet thickening, signal alteration, and ill-distinction was significantly different between the patients with PPP and those without, for both readers (p ≤ 0.020). Conversely, the presence of transiting nerve rootlet displacement, epidural fibrosis, and intrathecal arachnoiditis was not significantly different between the two groups (p ≥ 0.128). Among the above radiologic findings, transiting nerve rootlet thickening and signal alteration were the most significant findings in the multivariable analyses (p ≤ 0.009). Conclusion: On MRI, PPP was associated with transiting nerve rootlet abnormalities, including thickening, signal alterations, and ill-distinction, but was not associated with epidural fibrosis or intrathecal arachnoiditis. The most relevant findings were the nerve rootlet thickening and signal alteration.

• Journal of Korean Neurosurgical Society
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• 제53권1호
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• pp.52-56
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• 2013

Four patients underwent lumbar surgery. In all four patients, the dura was minimally torn during the operation. However, none exhibited signs of postoperative cerebrospinal fluid leakage. In each case, a few days after the operation, the patient suddenly experienced severe recurring pain in the leg. Repeat magnetic resonance imaging showed transdural nerve rootlets entrapped in the intervertebral disc space. On exploration, ventral dural tears and transdural nerve rootlet entrapment were confirmed. Midline durotomy, herniated rootlet repositioning, and ventral dural tear repair were performed, and patients' symptoms improved after rootlet repositioning. Even with minimal dural tearing, nerve rootlets may become entrapped, resulting in severe recurring symptoms. Therefore, the dural tear must be identified and repaired during the first operation.

• Advances in Traditional Medicine
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• 제6권2호
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• pp.102-107
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• 2006

The methanol extract and residual methanol fraction of Excoecaria agallocha L. (Euphorbiaceae) stem bark was investigated in this study by wheat rootlet and shoot growth inhibition, and antimicrobial bioassay. The methanol extract and residual methanol fraction showed high inhibitory effect on both the wheat rootlet (82-89%) and shoot growth (85-90%) compared to control. The methanol extract showed a better and dose related inhibition on both the rootlet and shoot growth compared to residual methanol fraction. The $IC_{50}$ value of methanol extract for rootlet and shoot were $2.88\;{\mu}g/ml$ and $2.32\;{\mu}g/ml$, and of residual methanol fraction for rootlet and shoot were $7.91\;{\mu}g/ml$ and $4.45\;{\mu}g/ml$. The methanol extract and residual methanol fraction did not show any antibacterial activity against the tested microorganisms of clinical isolates Escherichia coli, Staphylococcus aureous, Pseudomonas aeruginosa, Proteus vulgaris and Bacillus subtilis. The plant has the potential to be a source of novel cytotoxic compound(s).

• 한국조경학회지
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• 제44권5호
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• pp.59-67
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• 2016

공공기관의 조경공사 설계시에는 일반적으로 조달청에서 고시하는 조경수목 가격을 반영하나, 수목 식재시 조달청에서 정한 세근발달 재배품이라는 규정이 거의 준수되지 않고 있는 실정이다. 규정을 준수하면서 하자율을 줄일 수 있는 조경수 생산의 신기술, 신제품인 지중 매립형 컨테이너 모듈의 성능을 평가하기 위하여 단근 후 3개월간의 세근 발생밀도, 세근발달 길이 및 굴취시 세근 유지율, 수목의 생육 지장 여부에 대하여 일반노지 단근과 컨테이너 용기 지상재배와 비교 연구하였으며, 그 결과는 다음과 같다. 첫째, 세근 발생의 밀도는 컨테이너 모듈을 사용한 조경수에서는 88%로 매우 높게 나타났으며, 일반 노지 단근은 64%로 상대적으로 낮았고, 컨테이너 용기를 이용한 지상재배 조경수는 고사하여 측정의 의미가 없었다. 둘째, 조경수의 세근발달과 세근 유지율은 컨테이너 모듈의 경우, 평균 길이는 10.4cm, 세근 유지율은 100%로서 굴취에 의한 세근의 손상은 없었으며, 노지 단근의 경우 평균 길이는 25.6cm, 세근 유지율은 56%로 컨테이너 모듈의 절반 수준으로 훼손이 큰 것으로 나타났다. 컨테이너 용기 조경수의 세근발달은 전혀 이루어지지 않았다. 셋째, 컨테이너 모듈과 일반 노지 단근의 경우, 단근 작업에 따른 수목의 고사 또는 왜소엽 등의 지장이 전혀 없었다. 반면에 컨테이너 용기의 경우, 거의 전량이 고사하여 수목생육에 지장이 심각한 것으로 확인되었다. 이상에서, 지중매립형 컨테이너 모듈의 성능을 평가해 본 결과, 세 가지 모델 중 가장 우수한 것으로 확인되었으며, 컨테이너 모듈이 여름철 부적기 식재나 생육조건이 불리한 환경에서 수목의 빠른 초기 활착을 유도함으로써 하자를 예방하고, 조달청 규정 등에서 제시하고 있는 원칙을 준수할 수 있는 최적의 대안이 될 수 있을 것으로 기대한다.

• Advances in Traditional Medicine
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• 제7권4호
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• pp.379-384
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• 2007

Two medicinal plant of Sundarbans mangrove forest has been tested for the evaluation of growth inhibitory and antibacterial activity. The methanol extract of Xylocarpus granatum stem bark showed potent wheat rootlet ($IC_{50}=0.01{\mu}g/ml$) and shoot ($IC_{50}=0.23{\mu}g/ml$) growth inhibitory activity in a concentration related manner. The growth inhibitory activity was markedly decreased in residual methanol extract. The methanol extract showed antibacterial activity (MIC > 3 mg/ml) against Bacillus subtilis, Staphylococcus aureous and Proteus vulgaris. The residual methanol extract did not show any antibacterial activity. The results suggest the bioactive principle(s) of Xylocarpus granatum may be relatively non polar compound(s). The methanol extract and residual methanol extract of Sarcolobus globosus stem showed poor wheat rootlet and shoot growth inhibitory activity and no antibacterial activity.

• 대한수의학회지
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• 제55권3호
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• pp.181-184
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• 2015

Ciliary rootlet coiled coil protein (CROCC), the structural component that originates from the basal body at the proximal end of the ciliary rootlet, plays a crucial role in maintaining the cellular integrity of ciliated cells. In the current study, we cloned Xenopus CROCC and performed the expression analysis. The amino acid sequence of Xenopus laevis was related to those of Drosophila, cow, goat, horse, chicken, mouse and human. Reverse transcription polymerase chain reaction analysis revealed that CROCC mRNA encoding a coiled coil protein was present maternally, as well as throughout early development. In situ hybridization indicated that CROCC mRNA occurred in the animal pole of embryo during gastrulation and subsequently in the presumptive neuroectoderm at the end of gastrulation. At tailbud stages, CROCC mRNA expression was localized in the anterior roof plate of the developing brain, pharyngeal epithelium connected to gills, esophagus, olfactory placode, intestine and nephrostomes of the pronephric kidney. Our study suggests that CROCC may be responsible for control of the development of various ciliated organs.

• 한국균학회지
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• 제39권3호
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• pp.223-226
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• 2011

송이버섯 생육지의 소나무세근으로부터 내생균이 분리되었다. 분리된 내생균을 rDNA-ITS 영역으로 동정한 결과 18종이 확인 되었으며, 이중에는 자낭균류 15종, 털곰팡이 아문 3종이 포함되어 있음이 확인 되었다. 본 연구의 내생균 중에는 Penicillium속 균주가 가장 높은 빈도로 생육하고 있음이 확인되었다.

• Archives of Pharmacal Research
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• 제24권3호
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• pp.214-218
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• 2001

The mechanisms responsible for the antidiabetic activity of both the white ginseng radix (Ginseng Radix Alba, GRA) and the rootlet (Cinseng Radix Palva, GRP) were investigated. After a four week oral administration, the fasting blood glucose levels in the GRA- and GRP-treated groups were lower when compared to the control group. To elucidate the hypoglycemic mechanism(s) of the ginseng radices, glucose absorption from the small intestine, hepatic hexokinase and glucose-6-phosphatase activities, in addition to PPAR-${\gamma}$ expression in adipose tissue were examined. The results strongly suggest that GRA can improve hyperglycemia in KKAy mice, possibly by blocking intestinal glucose absorption and inhibiting hepatic glucose-6-phosphatase, and GRP through the upregulation of adipocytic PPAR-$\gamma$ protein expression as well as inhibiting intestinal glucose absorption.

• The Korean Journal of Physiology and Pharmacology
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• 제6권1호
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• pp.57-61
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• 2002

The renoprotective activities of white ginseng radix and rootlet were compared in spontaneously hypertensive rat (SHR) with diabetes. During oral administration of white ginseng radix (Ginseng Radix Alba, GRA) and white ginseng rootlet (Ginseng Radix Palva, GRP) for four weeks, arterial blood pressure and blood glucose levels were determined at every 10 days. In both GRA- and GRP-treatment groups, arterial blood pressures started to go down after 10 days of administration and maintained throughout the study period. After four weeks administrations of GRA and GRP, diastolic blood pressures were significantly decreased with 17% and 9%, respectively. GRA treatment also decreased blood glucose levels after 10 days of administration when compared with diabetic SHR group. At the end of the experiment, serum creatinine (Scr) and blood urea nitrogen (BUN) were not significantly different between the groups, except 62% higher value of BUN in diabetic SHR group when compared with SHR group. In the diabetic SHR group, the excretion of urinary albumin was increased significantly when compared with SHR. The level of urinary albumin in GRA treated group was markedly reduced when compared with diabetic SHR group $(67.8{\pm}4.7\;vs.\;131.3{\pm}13.5\;mg/24\;h).$ To examine the effects of ginseng radices on an overt diabetic nephropathy, index of kidney hypertrophy and transforming growth $factor-{\beta}1\;(TGF-{\beta}1)$ protein levels were evaluated. The glomerular and tubular cells stained positive for $TGF-{\beta}1$ seemed to be more abundant in diabetic SHR than in those with SHR, and GRA treated rats showed somewhat less $TGF-{\beta}1$ protein in glomerular and tubular cells when compared with diabetic SHR. Our results suggest that GRA might be a useful antihypertensive and antidiabetic agent with renoprotective effect.

• 생약학회지
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• 제41권3호
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• pp.185-189
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• 2010

In this study, we analyzed and quantified the amounts of bioactive phenolic constituents, xanthotoxin (1), oxypeucedanin (2), and imperatorin (3) in each part of the root of Angelica dahurica by HPLC, which validated by ICH guide lines comparing the linearity, intra day precision, inter-day precision. As a result, the amount of imperatorin 2.96% in the rootlet was two fold higher than that of the main root 1.32%. On the other hand, the amounts of xanthotoxin and oxypeucedanin in the rootlet showed similar to those of main root. In addition, the cortex of root is more plentiful of three consitituents (0.66%, 0.53%, and 1.85%) than those of xylem (0.29%, 0.05%, and 0.07%). These results show that the rootlet and cortex contain a large amount of bioactive phenolic constituent including xanthotoxin, oxypeucedanin, and imperatorin than other parts of the root.