• The Plant Pathology Journal
• /
• v.25 no.2
• /
• pp.113-120
• /
• 2009

Microscopic study of chili pepper (Capsicum annuum L.) infected with Phytophthora capsici, causing Phytophthora blight of chili pepper, was conducted to compare histological and cytological characteristics in the root and stem of susceptible (C. annuum cv. Bugang) and resistant (C. annuum cv. CM334) pepper cultivars. The susceptible pepper roots and stems were extensively penetrated and invaded by the pathogen initially into epidermal cells and later cortical and vascular cells. Host cell walls adjacent to and invaded by the infecting hyphae were partially dissolved and structurally loosened with fine fibrillar materials probably by cell wall-degrading enzymes of the pathogen. In the resistant pepper, the pathogen remained on root epidermal surface at one day after inoculation, embedded and captured in root exudation materials composed of proteins and polysaccharides. Also the pathogen appeared to be blocked in its progression at the early infection stages by thickened middle lamellae. At 3 days after inoculation, the oomycete hyphae were still confined to epidermal cells of the root and at most outer peripheral cortical cells of the stem, resulting from their invasion blocked by wound periderms formed underneath the infection sites and/or cell wall appositions bounding the hyphal protrusions. All of these aspects suggest that limitation of disease development in the resistant pepper may be due to the inhibition of the pathogen penetration, infection, invasion, and colonization by the defense structures such as root exudation materials, thickened middle lamellae, wound peridems and cell wall appositions.

• Journal of Microbiology and Biotechnology
• /
• v.26 no.3
• /
• pp.549-557
• /
• 2016

Aphids are a large group of hemipteran pests that affect the physiology, growth, and development of plants by using piercing mouthparts to consume fluids from the host. Based an recent data, aphids modulate the microbiomes of plants and thereby affect the overall outcome of the biological interaction. However, in a few reports, aboveground aphids manipulate the metabolism of the host and facilitate infestations by rhizosphere bacteria (rhizobacteria). In this study, we evaluated whether aphids alter the plant resistance that is mediated by the bacterial community of the root system. The rhizobacteria were affected by aphid infestation of pepper, and a large population of gram-positive bacteria was detected. Notably, Paenibacillus spp. were the unique gram-positive bacteria to respond to changes induced by the aphids. Paenibacillus polymyxa E681 was used as a rhizobacterium model to assess the recruitment of bacteria to the rhizosphere by the phloem-sucking of aphids and to test the effect of P. polymyxa on the susceptibility of plants to aphids. The root exudates secreted from peppers infested with aphids increased the growth rate of P. polymyxa E681. The application of P. polymyxa E681 to pepper roots promoted the colonization of aphids within 2 days of inoculation. Collectively, our results suggest that aphid infestation modulated the root exudation, which led to the recruitment of rhizobacteria that manipulated the resistance of peppers to aphids. In this study, new information is provided on how the infestation of insects is facilitated through insect-derived modulation of plant resistance with the attraction of gram-positive rhizobacteria.

• Korean Journal of Soil Science and Fertilizer
• /
• v.40 no.4
• /
• pp.280-291
• /
• 2007

Much research has been conducted in the field of phytoremediation since the discovery of the range of plants known as hyperaccumulators. Research has focused simultaneously on elucidating the mechanism of metal(loid) accumulation and development of practical techniques to enhance accumulation efficiency. To date, it is generally understood that there are five specific mechanisms employed by hyperaccumulating plant species that are either not or under utilized by non-hyperaccumulators. These include 1) enhanced metal(loid)s uptake through the root cell, 2) enhanced translocation in plant tissue, 3) detoxification and sequestration, 4) enhanced metal availability in soil:root interface, and 5) active root foraging toward metal(loid) enriched soils. Among these mechanisms, understanding of the plant-root effect on metal(loid) dynamics and subsequent plant uptake is vital to overcome the inherit limitation of phytoremediation caused by low metal(loid) solubility in soils. Plant roots can influence the soil chemistry in the rhizosphere through changes in pH and exudation of organic compounds such as low-molecular-weight organic acids (LMWOAs) which consequently change metal(loid) solubility. The decrease in soil pH by plant release of $H^+$ results in increased metal solubility. Elevated levels of organic compounds in response to high metal soil concentrations by plant exudation may also increases metal concentration in soil solution through formation of organometallic complexes.

• Journal of Periodontal and Implant Science
• /
• v.39 no.3
• /
• pp.375-381
• /
• 2009

Purpose: Root resection can be a valuable procedure when the tooth in question has a high strategic value. The prognosis of root resection has been well documented in previous studies, but the results focused on the palatal root resection have not been discussed in depth. I represent here the short term effectiveness of palatal root resection of maxillary first molars. Methods: Palatal root resection was performed on maxillary first molars of three patients. All the palatal roots were floating state on the radiographic finding and showed full probing depth and purulent exudation at initial examination. Reduction of palatal cusp and occlusal table was performed concomitantly. Endodontic therapy was completed after root resection. Results: Compromised maxillary first molars were treated successfully by palatal root resection in 3 cases. The mobility of resected tooth was decreased a little bit. The probing pocket depth of remaining buccal roots was not increased compared to initial depth. All the patients satisfied with comfort and cost effective results and the fact they could save their natural teeth. Conclusions: Within the above results, palatal root resection is an effective procedure treating compromised maxillary first molar showing advanced palatal bone loss to root apex with or without pulp involvement when proper case selection is performed.

• Korean Journal of Soil Science and Fertilizer
• /
• v.30 no.3
• /
• pp.257-264
• /
• 1997

To find out the influence of different rice varieties on the emission of methane in the soil and the exudation of carbohydrates from root, the experiment was conducted on a fine silty, mesic family of Aeric Fluventinc Haplaquepts (Jeonbug series). The varities of rise involved one early maturing variety of Japonica type(namweon) and three mid-to-late maturing varieties of Japonica type and one mid-to-late variety of Indica${\times}$Japonica crossed. The methane flux over the rice canopy was measured according to the closed chamber method modified by Shin and the methane concentration in the soil was measured using porous cup installed in the soil. The carbohydrate exudates from root were measured under nutrient solution culture. It was found that the methane flux tended to be lower in early maturing varieties than in the mid-to-late maturing varieties, lower in indica${\times}$Japonica crossed variety than in Japonica type varieties. There was positive correlation between the number of tillers and the weight of roots of rice plant and methane flux. The correlation, however, tended to be greater between the weight of roots and methane flux. There was no significant difference in the concentration of methane in the soil under different varieties of rice, excepting one variety, Kehwa under which methane concentration was highest. In case of carbohydrate exudation, early maturing variety tended to be higher than other varieties, although the opposite was the case in methane flux.

• Korean Journal of Weed Science
• /
• v.15 no.2
• /
• pp.131-140
• /
• 1995

This experiment was conducted to test the effect on germination and seedling growth of major industrial crops and weed control potential using alfalfa plant. When dried alfalfa residues were mixed into vermiculite, germination, length of shoot and root of crops, such as Perilla frutescens, Sorghum nevosum, Platycodon grandiflorum and weeds, Digitaria saguinalis, Setaria viridis, Siegesbechia pubescens, Ammaranthus lividus, and Solanum nigrum, were significantly inhibited as the dried residue concentration increased. More than 10% concentration of the dired residue caused 80% germination and growth inhibition. The fresh alfalfa exudation also inhibited the germination and seedling growth of crop, barley, rye, alfalfa, and sesame, and weeds, Echinochloa crus-galli, Siegesbechia viridis, and Portulaca oleracea. The degree of inhibition showed the different response according to the fresh exudation concentration, types of crops and weeds. Generally, as the exudation concentration increased, the germination and seedling growth of crops and weeds inhibited. The exudation of dried residue also exhibited the strong inhibition effect on germination and seedling growth of crops, alfalfa, Platycodon grandiflorum, barley, sesame, rye and weeds, D. sanguinalis, S. pubescens, S. viridis, P. oleracea, E. crus-galli, At the 10% concentrations, S. pubescens, and P. oleracea were not germinated and showed only 15% germination in the S. viridis. From this study, would conclude that alfalfa plant contained water soluble phytotoxic substances which were inhibitory to weeds and crops. This results suggest that alfalfa had some possibility to control some weed species using toxic compounds like natural herbicide.

• Korean Journal of Environmental Agriculture
• /
• v.28 no.2
• /
• pp.146-157
• /
• 2009

The influence of Pb-citrate complex formation on Pb uptake and the effect of Pb on organic acid exudation were investigated using four plant species, viz., sunflower (Helianthus annuus L), Indian mustard (Brassica juncea), canola (Brassica napus) and vetiver grass (Vetiveria zizanioides) under hydroponic conditions. Seedlings were exposed to different levels of Pb and Pb-citrate for 24 hrs and subsequently Pb distributions in plant shoot, root and hydroponic solution were measured. The dissolved organic carbon (DOC) concentration generally decreased as the concentration of Pb in the hydroponic solution increased. In contrast to DOC, the total organic acid concentrations exuded from Indian mustard roots significantly increased (424 to 6656 mg $kg^{-1}$) with increased Pb treatment, implying that exuding organic acids were involved in Pb accumulation in Indian mustard. The complexation of Pb with citrate enhanced Pb accumulation in the above ground portions. Lead concentration in Indian mustard increased from 2.05 mg $kg^{-1}$ to 6.42 mg $kg^{-1}$ when the concentration of citrate in solution increased from 0 to 50 mg $L^{-1}$. This result showed enhanced translocation of Pb from root to shoot with observation of transfer coefficient ($K_t$) increase from 2.03E-3 to 5.72E-3.

• Korean Journal of Environmental Agriculture
• /
• v.27 no.3
• /
• pp.279-284
• /
• 2008

We investigated whether carboxylate exudation of Brassica pekinensis Rupr. was affected by nitrate deposition from simulated acid rain. A gas chromatographic (GC) analysis was employed for the determination of low molecular weight organic acids (LOA) in rhizosphere soils, bulk soil, roots and leaves of Brassica pekinensis Rupr.. Rhizosphere soils were collected after 8 weeks of plant growth by first removing the bulk soil from the root system and then by mechanical move off the rhizosphere soil that adhered to the root surface with soft brush. Soil and plant materials were simultaneously extracted with the mixture of methanol and sulfuric acid (100:7, v/v). Seven organic acids, oxalic, malonic, fumaric, succinic, maleic, L-malic and citric acid were identified and quantified by GC equipped with FID. Oxalic, L-malic, and citric acids were found in both the bulk and rhizosphere soils, while most LOAs were not detected in the control treatment. On the contrary, except maleic acid, all other organic acids were detected in the leaves and roots of cabbages treated with nitrate deposition.

• Journal of Forest and Environmental Science
• /
• v.31 no.2
• /
• pp.109-118
• /
• 2015

Allelopathy is an ecological phenomenon that refers to the beneficial or harmful effects of one plant on another plant, both crop and weed species, by the release of organic chemicals (allelochemicals) from plant parts by leaching, root exudation, volatilization, residue decomposition in soil and other processes in both natural and agricultural systems. Allelopathy can affect many aspects of plant ecology including occurrence, growth, plant succession, the structure of plant communities, survival, dominance, diversity, and plant productivity. In this review, we describe the concept of allelopathy, some mechanisms of operation within plants and then focus on a select number of gymnospermous tree genera: Ephedra, Pinus, Taxus, Cedrus, Juniperus, Picea, Cunninghamia and Araucaria. Pinus, Taxus (yew) and Cedrus (cedar) trees have a strong negative allelopathic effect on the germination, growth, or development of other plant species in the forest community.

• Korean Journal of Soil Science and Fertilizer
• /
• v.41 no.1
• /
• pp.55-64
• /
• 2008

A detailed understanding and appreciation of the important mechanisms operating at the soil:root interface, commonly identified as the rhizosphere, is critical for evaluating the potential for particular plant species to be successfully used as part of a phytoremediation technique. For specific plants, mechanisms may exist to overcome the inherit limitation of the phytoremediation technique when poorly mobile soil metals are of interest. In the present study, the influence of root exudates on the rhizosphere chemistry of soil and consequential metal uptake were investigated following culture of vetiver grass (Vetiveria zizanioides), recognized as a promising plant for land stabilization, in three different long-term contaminated soils and one non-contaminated control soil. The soil solution pH increased (0.3-1.1 units) following vetiver grass culture and dissolved organic carbon (DOC) also significantly increased in all soils with the highest increase in PP02 (23 to $173mg\;L^{-1}$). Chemical changes are contributed to root exudation by vetiver grass when exposed to high concentration of heavy metals. Chemical changes, consequently, influenced metal (Cd, Cu, Pb, and Zn) solubility and speciation in the rhizosphere. The highest solubility was observed for soil Ko01 (eg. 2091 and $318{\mu}g\;L^{-1}$ for Cd and Pb, respectively). Initial heavy metal solubility in soils varied with soil and either increased or decreased following vetiver grass culture depending on the soil type. An increase in pH following plant culture generally resulted in a decrease in metal solubility, while elevated DOC due to root exudation resulted in an increase in metal solubility via the formation of metal-DOC complexes. Donnan speciation demonstrated a significant decrease in free Cd and Zn in the rhizosphere and the concentration of Cd, Pb, and Zn in vetiver grass shoot was highly correlated with soluble concentration rather than total soil metal concentration.