• Title/Summary/Keyword: Root density

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The Immunohistochemical Changes of Skin during Hair Follicle Cycle after Depilation in Mice

  • ;이창현
    • 대한의생명과학회지
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    • 제16권4호
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    • pp.349-357
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    • 2010
  • We have examined the histological changes of skin during hair follicle growth after depilation in C57BL/6N mice. We first studied on histological changes of number of mast cells and thickness of skin during hair follicle growth periods (telogen, 1 day, 3 day, 5 day, 10 day, 14 day, 17 day and 21 day after depilation) by toluidine blue, Giemsa and H&E staining methods. We second studied immunoreactive density of cytokines and Brdu labeled cells in skin during hair follicle growth periods after depilation in C57BL/6N mice by immunohistochemical methods. The histological changes on skin thickness was increased from telogen to 14 day. The number of mast cells was decreased in 3,5 and 10 day and increased in 14, 17 and 20 day after depilation. Immunoreactive density of cytokines [protein kinase C-${\alpha}$ (PKC-${\alpha}$), c-kit, and vascular endothelial growth factor (VEGF)] in 1, 3, 5, 10, and 14 day after depilation was mildly stained in bulge and cutaneous trunci m., but immunoreactive density of cytokines in 17 and 21 day was heavily stained in epidermis, bulge, outer root sheath (ORS), inner root sheath (IRS) and cutaneous trunci m.. Immunoreactive density of Brdu labeled cells in skin in 1 and 3 day was heavily stained in bulge, epidermis and connective tissue under the cutaneous trunci m.. In all periods, immunoreactive density of Brdu labeled cells in skin was heavily stained in bulge, subcutaneous tissue, cutaneous trunci m, ORS and IRS. These experiments suggest that histological changes related to hair follicle growth elevated mast cell counts, skin thickness and epidermis thickness and heavily stained immunoreactive density of cytokines and Brdu labeled cutaneous trunci m. and connective tissue under the cutaneous trunci m. after depilation in C57BL/6N mice.

수변구역 조성 녹지의 모니터링을 통한 식재방안 모색 - 가평군 시공지를 대상으로 - (Exploring Planting Strategies through Monitoring of a Greenspace Established in the Riparian Zone - The Case of an Implementation Site in Gapyeong County -)

  • 조현길;박혜미
    • 한국환경과학회지
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    • 제25권12호
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    • pp.1689-1699
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    • 2016
  • The growth conditions of planted trees, invasion of nuisance herbaceous species, competition between species, and effects of erosion control were monitored over five years in a riparian greenspace in Gapyeong County that was established through multilayered and grouped ecological planting. Of 156 trees planted in the upper and middle layers, 5.8% died. This tree death was attributed to poor drainage or aeration in the rooting zone from the clay-added root ball and too deep planting as well as a small-sized root ball and scanty fine roots. Of all the trees, 21.6% grew poorly due to transplant stress in the first year after planting, but they started to grow vigorously in the third year. This good growth was largely associated with soil improvement before planting, selection of appropriate tree species based on growth ground, and control of dryness and invasive climbing plants through surface mulching and multilayered/grouped planting. Mixed planting of fast-growing species as temporary trees was desirable for accelerating planting effect and increasing planting density. Thinning of fast-growing trees was required in the fifth year after planting to avoid considerable competition with target species. To reduce the invasion of herbaceous and climbing plants that oppress normal growth of planted trees, higher density planting of trees (crown opening of about 15%), woodchip mulching to a 10-cm depth, and edge planting 2 m wide were more effective than lower density planting (crown opening of 70%), no surface mulching, and no edge planting, respectively. This reduction effect was especially great during the first three years after planting. Nuisance herbaceous plants rarely invaded higher density planting with woodchip mulching over the five years. Higher density planting or woodchip mulching also showed much greater erosion control through rainfall interception and buffering than lower density planting with no mulching did. Based on these results, desirable planting and management strategies are suggested to improve the functions of riparian greenspaces.

국산(國産) 파티클보드와 중밀도섬유판의 나사못유지력(維持力)(II) -나사못 유지력(維持力) 예측식(預測式)- (Holding Strength of Screw in Domestic Particleboard and Medium Density Fiberboard(II) -Predicting Formula of Withdrawal Strength of Screw-)

  • 이필우;박희준;한유수
    • Journal of the Korean Wood Science and Technology
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    • 제19권4호
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    • pp.43-51
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    • 1991
  • This study was carried out to determine the withdrawal strength of various screws according to root diameter of screw and embeded length on the face and edge of domestic particleboard and medium density fiberboard. The obtained results were as follows: 1. The withdrawal strength of screw in domestic particleboard and medium density fiberboard was closely related to embeded length of the screw but less dependent on root diameter of the screw. 2. The withdrawal strength on the face and edge of domestic particleboard could be predicted by means of the following expression: $F_{Pf}=4.60{\times}D^{0.24}{\times}L^{1.14}(R^2=0.87)$ $F_{Pe}=0.54{\times}D^{0.43}{\times}L^{1.73}(R^2=0.84)$ Where: $F_{Pf}$ : withdrawal strength on the face of particleboard(kgf) $F_{Pe}$=withdrawal strength on the edge of particleboard(kgf) D=diameter of the screw(mm) L=embeded length(mm) 3. The withdrawal strength on the face and edge of domestic medium density fiberboard could he predicted by means of the following expression: $FM_f=1.53{\times}D^{0.53}{\times}L^{1.39}(R^2=0.93)$ $F_{Me}=1.14{\times}D^{0.66}{\times}L^{1.36}(R^2=0.87)$ where: $F_{Mf}$ = withdrawal strength on the face of medium density fiberboard(kgf) $F_{Mf}$=withdrawal strength on the edge of medium density fiberboard(kgf).

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갯버들 근계의 토양 입자 해리 억제효과에 관한 연구 (A Study on the Reduction of Soil Particle Dissociation Rate by the Root of 'Salix Gracilistyla')

  • 이춘석;박명안;강호철
    • 한국환경복원기술학회지
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    • 제6권3호
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    • pp.69-78
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    • 2003
  • The main purpose of this study was to verify the shore margin protection effect of the root system of Salix gracilistyla Miq. developed from direct sticking cuttings on wetland, focusing on the effect of the root system reducing soil particle dissociation rate in water. The soil dissociation rate was examined through slaking tests with cylindric pure soil column at maximum particle density and the same size column of root reinforced soil. The dry weight of remained soil was measured after 5, 10, 15, 30minutes and 1, 6, 12, 24, 48hours inundation. As results, the soil particles began to dissociate severely at 10 minutes and only 10% of soil particles were left after 25minutes inundation. The stable slope angle of pure soil was $36^{\circ}$after 24 hours. On the other hand, the columns of root reinforced soil were stable even after 24hours, being dissociated only 7.2% of soil particles. So, it was revealed that the root system was very effective materials protecting more than 80% of soil particle from dissociation in inundation.

약용식물의 뿌리혹선충 발생과 분류동정 (Incidence, and Identification of Three Root-Knot Nematode species Occurring in the Medicinal Herbs)

  • 박소득;;김재철;최부술;김탁
    • 한국식물병리학회지
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    • 제14권6호
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    • pp.603-605
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    • 1998
  • Soil and root samples were collected form the rhizoshpere of 11 different medicinal plants to determine the incidence, density and identification of root-knot nematode species associated with medicinal herbs. About 55% of medicinal herbs examined was found to be infested with root-knot nematodes. As a result of infection casued by three root-knot nematodes, M. hapla recorded 43.3% in medicinal herba whereas M. incognita and M. arenaria showed 7.9% and 3.7%, repectively. Forsythia koreana, Hemerocalis fulva, Hibuscus mutabilis and Petasites japonicus were the most severely infested herbs whereas Acanthopanax sessilflorus was least infested. Population of the second stage younger plants. Meloidogyne hapla, M. incognita and M. arenaria were the species associated with the medicinal herbs. The most abundant nematode observed in medicinal herbs was M. hapla and followed by M. incognita and M. arenaria. M. arenaria was observed firstly on Ficus carica, one of medicinal plant.

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토성(土性)과 용적밀도(容積密度)가 대두(大豆)의 뿌리 생장특성(生長特性)과 양분흡수기능(養分吸收機能)에 미치는 영향(影響) (Influence of Soil Texture and Bulk Density on Root Growth Characteristics and Nutrient Influx Rate of Soybean Plant)

  • 정영상;임형식
    • 한국토양비료학회지
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    • 제22권3호
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    • pp.221-227
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    • 1989
  • 토성(土性)이 다른 두 토양(土壤)의 압밀상태(壓密狀態)가 대두(大豆)의 근생장특성(根生長特性)과 양분(養分)의 흡수기능처리(吸收機能差異)를 알아보기 위하여 용적밀도(容積密度)와 수분상태(水分狀態)를 달리한 Core에서의 유근신장(幼根伸長)과 용적밀도(容積密度)를 달리하여 pot재배(栽培)하였을 때 근(根)의 총(總)길이와 표면적(表面積), 근반경(根半徑) 및 양분(養分)의 흡수속도(吸收速度)를 조사(調査)한 결과(結果) 다음과 같다. 1. 대두(大豆)의 유근신장(幼根伸長)은 용적밀도(容積密度)가 낮을수록 빨랐으며 양토(壤土)에서는 토양수분(土壤水分) 상태(狀態)가 - 4bar 이하(以下)에서, 사양토(砂壤土)에서는 - 1bar 이하(以下)에서 급격(急激)히 감소(減少)하였다. 2. 유근(幼根)의 신장(伸長)은 ELE경도계(硬度計) 토양강도(土壤强度) 2.0 이상(以上)에서 급격(急激)히 감소(減少)하였으며, 근신장속도(根伸長速度)와 토양강도간(土壤强度間)에는 2차식(次式)으로 표시(表示)되었다. 그러나 사양토(砂壤土)에서 -10bar이하(以下)의 토양수분상태(土壤水分狀態)에서는 경도(硬度)에 관계(關係)없이 근신장속도(根伸長速度)가 낮았다. 3. pot재배시험(栽培試驗) 결과(結果), 뿌리의 총(總)길이는 용적밀도(容積密度)가 $1.2g/cm^3$인 양토(壤土)에서 가장 길었으며, $1.4g/cm^3$인 사양토(砂壤土)에서 가장 짧았다. 세근(細根)의 평균직경(平均直徑)은 사양토(砂壤土)에서 생육(生育)한 것이 양토(壤土)에서 생육(生育)한 것보다 굵었다. 뿌리의 총표면적(總表面積)은 용적밀도(容積密度)가 낮을수록 넓었으며, 양토(壤土)에서 사양토(砂壤土)보다 넓었다. 4. 양분(養分)의 흡수량(吸收量)은 건물생산(乾物生産)이 많은 양토(壤土)에서 많았다. 단위(單位)뿌리표면적당(表面積當) 흡수속도(吸收速度)는 질소(窒素)가 양토(壤土)에서 $597{\sim}753n\;mole/day{\cdot}cm^2$, 사양토(砂壤土)에서 $222{\sim}365n\;mole/day{\cdot}cm^2$이었으며, 용적밀도(容積密度)가 높은 토양(土壤)에서 높은 값을 보였다. K는 $99{\sim}175n\;mole/day{\cdot}cm^2$ P는 $26{\sim}46n\;mole/day{\cdot}cm^2$이었고, Ca와 Mg는 각각(各各) $175{\sim}230n\;mole/day{\cdot}cm^2$, $163{\sim}205n\;mole/day{\cdot}cm^2$의 범위(範圍)를 보였으며 토양간(土壤間)의 차이(差異)는 질소(窒素)보다 적은 경향(傾向)이었다.

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년근별 인삼추출물의 사포닌함량, 물리성 및 색도의 변화 (Variation of Effective Constituents Contents, Physical Properties and Color Intensities of Extracts from White Ginseng Roots of Different Cultivating Years.)

  • 김동철;장상문;최정
    • Applied Biological Chemistry
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    • 제38권1호
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    • pp.67-71
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    • 1995
  • 인삼추출물의 품질은 유효성분인 사포닌의 함량뿐만 아니라 추출물의 물리성 및 색도도 주요한 요인이다. 따라서 년근별 원료삼에서 추출한 추출물의 사포닌함량, 물리성 및 색도를 조사함으로써, 인삼추출물 제조시 우량원료삼을 선택을 위한 기초자료를 제시하고자 본 연구를 수행하였다. 원료삼의 년수가 증가할수록 추출물 중의 전당 및 sucrose의 함량과 추출물의 수율은 증가하였으나 조saponin함량은 년근 간에 유의성이 없었다. 조회분함량은 4년근의 추출물에서 가장 많았으며, 5년근의 추출물에서 가장 낮았다. 또한 인삼추출물의 탁도는 인삼근의 년수가 증가할수록 감소되었으나, 점도는 년근별 유의차가 인정되지 않았다. 갈변반응의 중간생성물의 흡광도는 인삼근의 년수가 증가할수록 감소되었으며, 5년근 추출물의 명도 및 황색도가 가장 높았다.

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Correlation between the Pressure Pain Threshold and Sonography and Spontaneous Electrical Activity in Myofascial Trigger Points

  • Kim, Hyun-Jin;Kim, Myung-Hoon;Kim, Su-Hyon;Oh, Seok;Choi, Ji-Ho;Kim, Tae-Youl
    • The Journal of Korean Physical Therapy
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    • 제22권3호
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    • pp.17-21
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    • 2010
  • Purpose: This study was designed to investigate possibilities for quantitative analysis using the electromyography and sonography. For better understanding, we evaluated the correlation between the pressure pain threshold and sonography, spontaneous electrical activity in trigger points located in the upper trapezius muscle. Methods: Thirty three active subjects volunteered to participate in this study (n=33). They had a palpable taut band, exquisite spot tenderness of a nodule in a taut band, spontaneous pain, referred pain, jump sign, local twitch response, and a painful limit to full stretch range of motion. We measured Pressure pain threshold, density, white area index, root mean square, and reaction. Pearson’s correlation coefficient was calculated to estimate the relationship between the pressure pain threshold and other variables including density, white area index, root mean square, and reaction time. Results: There were significant correlations between pressure pain threshold and density (r=-0.75, p<0.01), and between pressure pain threshold and white area index (r=-0.74, p<0.01). A significant correlations between pressure pain threshold and root mean square (r=-0.59, p<0.01). The significant correlation was found between pressure pain threshold and reaction time (r=-0.64, p<0.01). Conclusion: These should indicate whether quantitative analysis can be done using the characteristics of electromyography and sonography.