• Title/Summary/Keyword: Root density

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Root Colonization and Quorum Sensing of the Antagonistic Bacterium Pseudomonas fluorescens 2112 involved in the Red-pepper Rhizosphere (생물방제균 Pseudomonas fluorescens 2112의 고추 근권정착능과 Quorum-sensing 기능)

  • Jung, Byung-Kwon;Kim, Yo-Hwan;Kim, Sang-Dal
    • Microbiology and Biotechnology Letters
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    • v.41 no.1
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    • pp.105-111
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    • 2013
  • Biofilm formation of multifunctional plant growth promoting rhizobacterium (PGPR), Pseudomonas fluorescens 2112 is necessary for P. fluorescens 2112 to have a positive impact on the rhizosphere of red-pepper. This study investigated whether signal molecules of the quorum sensing AHLs are produced in order to confirm biofilm formative ability. Through the use of Petri dish bioassays a blue circle formed evidence of AHLs. It was confirmed that P. fluorescens 2112 produced six-carbon-chain-long AHLs by TLC bioassay. The bacterial density of P. fluorescens 2112 on the top and bottom of pepper plant roots was estimated as $3{\times}10^5$ and $8{\times}10^3$ CFU/g root, respectively. P. fluorescens 2112 exist more with high-density of $3.5{\times}10^6$ CFU/g soil at a depth of 1 cm but at a low-density of $1.1{\times}10$ CFU/g soil at a depth of 5 cm, from the surface of rhizosphere soil. In addition, biofilm formation of P. fluorescens 2112 on the epidermises and the tips of the red-pepper roots were confirmed visually by SEM. Thus, the production of AHLs by P. fluorescens 2112 brings about quorum sensing signaling and the formation of biofilm on the roots which has a positive effect on economically important crops such as red-pepper by additionally producing a variety of antifungal substances and auxin.

A Modulation and Channel State Estimation Algorithm Using the Received Signal Analysis in the Blind Channel (블라인드 채널에서 수신 신호 분석 기법을 사용한 변조 및 채널 상태 추정 알고리즘)

  • Cho, Minhwan;Nam, Haewoon
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.41 no.11
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    • pp.1406-1409
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    • 2016
  • In this paper, we propose the heuristic signal grouping algorithm to estimate channel state value over full blind communication situation which means that there is no information about the modulation scheme and the channel state information between the transmitter and the receiver. Hereafter, using the constellation rotation method and the probability density function(pdf) the modulation scheme is determined to perform automatic modulation classification(AMC). Furthermore, the modulation type and a channel state value estimation capability is evaluated by comparing the proposed scheme with other conventional techniques from the simulation results in terms of the symbol error rate(SER) and the root mean square error (RMSE).

Design of Tower Damper Gain Scheduling Algorithm for Wind Turbine Tower Load Reduction (풍력터빈 타워 하중 저감을 위한 타워 댐퍼 게인 스케줄링 알고리즘 설계)

  • Kim, Cheol-Jim;Kim, Kwan-Su;Paek, In-Su
    • Journal of the Korean Solar Energy Society
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    • v.38 no.2
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    • pp.1-13
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    • 2018
  • This paper deals with the NREL (National Renewable Energy Laboratory) 5-MW reference wind turbine. The controller which include MPPT (Maximum power point tracking) control algorithm and tower load reduction control algorithm was designed by MATLAB Simulink. This paper propose a tower damper algorithm to improve the existing tower damper algorithm. To improve the existing tower damper algorithm, proposed tower damper algorithm were applied the thrust sensitivity scheduling and PI control method. The thrust sensitivity scheduling was calculated by thrust force formula which include thrust coefficient table. Power and Tower root moment DEL (Damage Equivalent Load) was set as a performance index to verify the load reduction algorithm. The simulation were performed 600 seconds under the wind conditions of the NTM (Normal Turbulence Model), TI (Turbulence Intensity)16% and 12~25m/s average wind speed. The effect of the proposed tower damper algorithm is confirmed through PSD (Power Spectral Density). The proposed tower damper algorithm reduces the fore-aft moment DEL of the tower up to 6% than the existing tower damper algorithm.

Root Colonization by Beneficial Pseudomonas spp. and Bioassay of Suppression of Fusarium Wilt of Radish (유용 Pseudomonas 종의 근면점유와 무우 Fusarium시들음병의 억제에 관한 생물학적 정량)

  • Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.25 no.1 s.80
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    • pp.10-19
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    • 1997
  • Fusarium wilt of radish (Raphanus sativus L.) is caused by the Fusarium oxysporum f. sp. raphani (FOR) which mainly attacks Raphanus spp. The pathogen is a soil-borne and forms chlamydospores in infected plant residues in soil. Infected pathogen colonizes the vascular tissue, leading to necrosis of the vascular tissue. Growth promoting beneficial organisms such as Pseudomonas fluorescens WCS374 (strain WCS374), P. putida RE10 (strain RE10) and Pseudomonas sp. EN415 (strain EN415) were used for microorganisms-mediated induction of systemic resistance in radish against Fusarium wilt. In this bioassy, the pathogens and bacteria were treated into soil separately or concurrently, and mixed the bacteria with the different level of combination. Significant suppression of the disease by bacterial treatments was generally observed in pot bioassy. The disease incidence of the control recorded 46.5% in the internal observation and 21.1% in the external observation, respectively. The disease incidence of P. putida RE10 recorded 12.2% in the internal observation and 7.8% in the external observation, respectively. However, the disease incidence of P. fluorescens WCS374 which was proved to be highly suppressive to Fusarium wilt indicated 45.6% in the internal observation and 27.8% in the external observation, respectively. The disease incidence of P. putida RE10 mixed with P. fluorescens WCS374 or Pseudomonas sp. EN415 was in the range of 10.0-22.1%. On the other hand, the disease incidence of P. putida RE10 mixed with Pseudomonas sp. EN415 was in the range of 7.8-20.2%. The colonization by FOR was observed in the range of $2.4-5.1{\times}10^3/g$ on the root surface and $0.7-1.3{\times}10^3/g$ in the soil, but the numbers were not statistically different. As compared with $3.8{\times}10^3/g$ root of the control, the colonization of infested ROR indicated $2.9{\times}10^3/g$ root in separate treatments of P. putida RE10, and less than $3.8{\times}10^3/g$ root of the control. Also, the colonization of FOR recorded $5.1{\times}10^3/g$ root in mixed treatments of 3 bacterial strains such as P. putida RE10, P. fluorescens WCS374 and Pseudomonas sp. EN415. The colonization of FOR in soil was less than that of FOR in root part. Based on soil or root part, the colonization of ROR didn't indicate a significant difference. The colonization of introduced 3 fluorescent pseudomonads was observed in the range of $2.3-4.0{\times}10^7/g$ in the root surface and $0.9-1.8{\times}10^7/g$ in soil, but the bacterial densities were significantly different. When growth promoting organisms were introduced into the soil, the population of Pseudomonas sp. in the root part treated with P. putida RE10 was similar in number to the control and recorded the low numerical value as compared with any other treatments. The population density of Pseudomonas sp. in the treatment of P. putida RE10 indicated significant differences in the root part, but didn't show significant differences in soil. The population densities of infested FOR and introduced bacteria on the root were high in contrast to those of soil. P. putida RE10 and Pseudomonas sp. EN415 used in this experiment appeared to induce the resistance of the host against Fusarium wilt.

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Growth of Landscape Tree Species at Two Planting Densities in a Planting Pilot System for Reclaimed Dredging Areas (임해준설매립지 식물재배공정에서 밀도에 따른 조경수목의 생장)

  • Lee, Deok-Beom;Nam, Woong;Kwak, Young-Se;Jeong, In-Ho;Lee, Sang-Suk
    • Journal of the Korean Institute of Landscape Architecture
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    • v.37 no.2
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    • pp.114-123
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    • 2009
  • To investigate the possible use of plants for landscaping in reclaimed soil, a planting pilot system experiment was performed over the course of four years in reclaimed dredging area with four species: Alnus firma, Alnus hirsuta, Pinus thunbergii, and Pyrachantha angustifolia for 4 years. The physicochemical characteristics of the tested soil showed that it was sandy through coming from a reclaimed dredging area. The average pH of the tested soil was 7.16(slight alkali), and electric conductivity(EC) was relatively low, $294{\mu}S/cm$, even though it came from a saltwater area. To test the effect of planting density vs. phytomass by plant specie from a planting basin, the experiment was designed using four plant species with high and low planting densities over 4 years. The planting conditions of the growth of landscape tree species exhibited growth height as follows: A. hirsuta, A. firma, P. thunbergii, and P. angustifolia, whill the DBH followed the order of A. hirsuta, A. firma, and P. thunbergii. The total phytomass of each plant was higher at low density planting areas than high density planting area in terms of total phytomass production and growth distribution in the reclaimed dredging area. Total phytomass per unit area increased as follows: A. hirsuta, A. firma, P. thunbergii, and P. angustifolia. The total phytomass per each tested plant was 2 times higher in low density planting areas than high density planting areas. Total phytomass per unit area, however, was similar or slighty higher in high density planting areas compared to low density areas. Among the tested plants, A. hirsuta showed the highest phytomass, implying that A. hirsuta adapted very well to the reclaimed area and has the capability of a fast growth, nitrogen fixation tree, and utilizing insoluble nutrients through inoculated root nodule bacteria. The yield of phytomass per individual in low density Alnus species was greater than that of the high density. However, those per unit areas had no difference in the density-dependent planting. The ratio of belowground to aboveground was $0.21{\sim}0.26$. Thus, it could be concluded that the Alnus species are potential candidates for ornamental tree species in reclaimed dredging areas. This study offers baseline data for the use of ornamental tree species in reclaimed dredging areas. Additional research is required for different ornamental species in order to increase phytomass of a planting conditions based on reclaimed dredging areas.

Treatment Effect of Mulberry Root-Bark (Mori radicis Cortex) Extract on Hypercholesterolemia in Rabbits

  • Kim Dong Kyu;Chai Hee-Youl;Kim Tae Myung;Lee Nam-Jin;Cho Jung-Hee;Park Jung-Hui;Kang Jong-Koo;Kim Yun-Bae;Hwang Seock-Yeon
    • Biomedical Science Letters
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    • v.11 no.3
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    • pp.383-388
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    • 2005
  • This study reports that the effect of Mori radicis cortex, Mulberry root-bark, on the lipid peroxidation products formation in the liver, blood serum and atherosclerosis of hypercholesterolemia rabbits fed Mulberry root-bark for eight weeks. The rabbits were permitted a fixed quantity to extract of Mulberry root-bark mixed hypercholesterol fodder. The lipid peroxidation products, malondialdehyde (MDA) was significantly diminished. Total cholesterol in serum worthily decreased. Also, triglyceride and high density lipoproteins (HDLs) increased after feeding Mori radicis cortex. Besides, thicky atheromatous plaque on the wall of aorta of rabbits fed on hypercholesterol diet were markedly attenuated by Mulberry root-bark extract. These results support and substantiate the extract of Mulberry root-bark as protection against lipid peroxidation in the liver, blood serum and atherosclerosis.

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The Effects of Soil Particle Composition on Soil Physical Properties and the Growth of Woody Plants (토양의 입도조성이 토양의 물리성 및 목본식물의 생장에 미치는 영향)

  • 이소정;김민수
    • Journal of the Korean Institute of Landscape Architecture
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    • v.25 no.2
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    • pp.54-61
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    • 1997
  • This study has conducted to analyze the crelationship among soil properties and to investigate how they affect soil physical characteristics and plant growth. The experiment of woody plant growth was conducted as follows : Type I was the original soil. Type II, the soil particles smaller than 20${\mu}{\textrm}{m}$ was removed from the original soil. Type III, the soil particles is smaller than 75${\mu}{\textrm}{m}$ was removed from original soil. Wisteria floribunda A.P.DC and Celtis sinensisi Pers. were used for plant growth measurement. 1. Soil type II. the closest to Fuller's curved line, showed high dry bulk density and low in soil pores and saturated hydraulic conductivities. This created poor soil aeration and limited space for the root to growth. When the root did not have sufficient space to grow, there was a lot of physical stress, which hindered the root growth. 2. Soil typeIII was high saturated hydraulic conductivity and a lot of soil pores larger than 10 ${\mu}{\textrm}{m}$. As a result, there were more available spaces for root to spread. It was considered that there was less physical stress for root growth. Therefore, soil typeIII showed significantly greater root growth. 3. Because soil type III has less small particles and saturated hydraulic conductivity was high, and water infiltrates rapidly into the underground when there was rainfall or irrigation. The soil typeIII becomes much stronger soil mechanically due to the less small particles. Therefore, soil typeIII was a suitable material for applying on planting sites where soil compaction is expected.

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Improvement of Market Quality of Minimally Processed Horticultural Products Using Ozone (오존수를 활용한 부분 가공 원예산물의 청정화 및 유통성 증진)

  • Hwang, Yong Soo;Jun, Il Yeul;Ku, Ja Hyeung
    • Korean Journal of Agricultural Science
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    • v.30 no.2
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    • pp.128-138
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    • 2003
  • The bacterial density of partially processed horticultural crops in the commercial market was relatively high probably due to improper handing or processing. Between crops, the bacterial density was low in peeled garlic and ranged above $10^8cfu{\cdot}g^{-1}fw$ in other crops. Especially in brake fern, the bacterial density was the highest and non food materials such as packing strips were found. There was difference in the effects of ozone-treated water washing treatment between crops. In root crops such as burdock and lotus root, ozone showed positive effect on preventing discoloration as well as lowing bacterial density resulting in the increase of marketing period. Any positive effect was not found when sliced crops were treated over 5 min at $0.3mg{\cdot}L^{-1}$ of dissolved ozone. In lettuce slices, leaf tissues were severely discolored and rapidly soften at $0.3mg{\cdot}L^{-1}$ for 2 min dipping. Ozone was highly effective on inhibiting bacterium propagation and off-flavor. This effect was more stronger in Fischer ligulariata than brake fern, probably due to the difference of tissue thickness. Results indicated that ozone-treated water washing had a strong potential to improve the market quality of partially processed horticultural crops including dried wild greens and sliced root crops.

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The Effect of Vermicast on the Growth of Vegetable Plants - Estimation of Optimum Mixture Ratios of Vermicast - (지렁이 분립이 엽채류의 생육에 미치는 영향 - 분립의 최적혼합비율의 추정 -)

  • Kim, In-Su;Kim, Sung-Jin;Lee, Ji-Young;Lee, Ju-Sam
    • Korean Journal of Organic Agriculture
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    • v.13 no.4
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    • pp.413-422
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    • 2005
  • This study was carried out to investigate the effect of different mixture ratios of vermicasts on the growth of leaf beet(Beta vulgaris var. cicla), young radish (Raphanus sativus L), spinach(Spinacia oleracea) and lettuce(Lactuca sativa) The mixture of ratios of vermicast(fermented pig manure with sawdust) and peatmoss were 100%+0%, 60%+40%, 40%+60% and 0%+100%(control), respectively. The bulk density, particle density, pH, electrolytic conductivity, ash, total nitrogen, cation exchange capacity, available phosphorous and exchangeable cations increased significantly compared to those grown in the 100% of peatmoss(control). The significantly higher values of plant length, leaf area, shoot dry weight, root weight, root length and biological yield obtained were $40{\sim}100%$ and 40% of vermicast in leaf beet and lettuce, respectively. The young radish and spinach showed that significantly differences of shoot dry weight, root weight and biological yield in the mixture ratios of vermicast were $40{\sim}60%$ and 40% in young radish and spinach, respectively.

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Use of Paper Mill Sludge and Briquet Ash as Root Zone Soil Mixtures for Thrfgrass Culture (잔디상토로서의 제지스럿지와 연탄재 이용에 관한 연구)

  • 구자영;김태일;안주원
    • Asian Journal of Turfgrass Science
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    • v.6 no.1
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    • pp.11-22
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    • 1992
  • To determine the use of waste materials as root zone soil mixtures for turfgrass culture, the effects of paper mill sludge and briquet ash on physical and chemical properties of soil and growth of turfgrasses were examined. Three turfgrass species of zoysiagrass(Zoysia japonicaSteud.). kentycky bluegrass(Poa pratensis L. 'Ram I') and creeping bentgrass(Agrostis panistris Huds 'Persucross') were cultured in 32cm diameter plastic pots containing various soil mixtures. The basic ingredients used for mixtures included sand(SD), field soil(SL), paper mill sludge(PS), sphagnum peat moss(PM) and briquet ash(BA). Seven combinations using these ingreients were mixed in different percentage by volume as follows: SD+SL+PM(80:10.10), SH+SL+PS(80:10:10), SD-PM(80:20), SD+PS (80:20), SD+BA(80:20), SD+BA+PM(60:20:20) and SD+BA+PS(60:20:20). 1. Paper mill sludge showed pH of 6.6, more than 30% of organic matter content, and higher concentrations of total N, P, k, Ca, Mg and CEC. Bulk density, fild moisture capacity and electrical conductivity of soil mixtures were increased by the comimation of 10~20% PS by volume. 2. Briquet ash showed pH of 8.0, and higher levels of P, k, Ca and Mg than those of field soiks. Bulk density, field moisture capacity and hardenss of soil mixtures were increased but vertical water flow rate and electrical conductivity were decreased by the combination of 20% BA by volume. 3. Phytotoxic effects of PS and BA on growth of turfgrasses were not found. Shoot growth of all three species was higher in soil combination of SD+BA+PS than that of SD+SL+PM added with fertilizer. However, root growth was better in soil mixtures combined with PM. Soil mixtureomposed of 60% SD, 20% BA and 20% PS by volume was most effective on growth of all three species. 4. Paper mill sludge resulted in higher N level in the leaf tissue. The contents of heavy metals such as Cd and Ph did not vary significantly among soil mixtures and species. However, the Mn level was 2~3 times higher in plants growh in mixtures containing PM compared with others, and especially it was higher in creeping bentgrass than other species.

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