• The Korean Journal of Zoology
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• v.31 no.1
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• pp.29-34
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• 1988

In order to investigate the role of nuclei and cytoplasm in early embryogenesis, interspecific nuclear transplantation was carried out Nuclei of one species of amphibian was transplanted into enucleated egg of another species. In interspecific hybrids between Rana species, development was arrested before or right after the dorsal lip formation. In intergenetic hybrids between Rana, Xenopus and axolod, developmental arrest took place at late blastula stage. Comparing the developmental capacity of each nudeocytoplasmic hybrid, the more distantly related the species, the earlier does development arrest. The general rule is that nuclear transfers between any amphibian species will form a regulary cleaved late blastula. Two plausible factors relate to limitation of tite developmental capacity of nudeocytaplasmic hybrids. One is an irreversible change in grafted nuclei and another is tunctional incompatibility between the recipient cytoplasm and transplanted nucleus. The later is postulated a more possible cause of the arrestrnent.

• Proceedings of the Safety Management and Science Conference
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• 2006.11a
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• pp.415-426
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• 2006

Many technical and nontechnical issues hinder enterprise wide workflow management. The most significant technical issue is the inability to deal with the heterogeneity among users, workflow types, and WFMSs. Not all users demand the same workflow functionality, so user interfaces of different levels of sophistication are required. Because workflow types cannot always be fully predefined, they often need to be adjusted or extended during execution. Unlike relational database management systems, however, each WFMS often has differing workflow metamodels. This leads to incompatibility between WFMSs, making integration into an environment comprising many heterogeneous WFMSs a troublesome and sometimes impossible task. Current Workflow system consists mainly of Database system. It contains some problems like that the integration relationship among system processes cant be expressed properly. This research has been focused on two phases that should be considered in the Workflow system. First of all, the first phase is the analysis phase; one of its role is to figure out independent execution task unit(Workflow component). The second phase is design phase that provides with the framework to execute these task units actively. The Workflow component extraction method in the analysis phase uses a analysis method called C-C Net and, in the design phase, the architecture that makes the these Workflow component executed actively is provided. Through this research, each process is divided into a task unit and more effective Workflow system could be formed by executing these units actively. Current system layer calls task units, on the other hand, the Workflow system this research implemented provides with the architecture that places a layer between them that controls task units actively.

• Clinical and Experimental Reproductive Medicine
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• v.47 no.2
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• pp.122-129
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• 2020

Objective: The survival of a semi-allogeneic fetus depends on several immunological mechanisms, and it has been suggested that recurrent pregnancy loss (RPL) could develop as a result of one or more immunological abnormalities. Methods: Compatibility between partners for human leukocyte antigen (HLA) genotypes and the relationships between maternal killer-cell immunoglobulin-like receptor (KIR) and paternal HLA-Bw4/Bw6 and HLA-C1/C2 supra-groups were investigated in 25 couples with RPL in comparison to healthy couples with children. HLA and KIR genotyping was performed using polymerase chain reaction with sequence-specific primers and/or sequence-specific oligonucleotides. Results: HLA class I incompatibility between partners, especially in HLA-B alleles, was more common in the RPL group (p= 0.01). HLA-C2 homozygosity was more frequent in the male partners of RPL couples than in other groups (p= 0.03). The KIR2DL5 gene frequency was significantly higher in both the female and male partners of RPL couples, whereas the KIR2DS3 gene frequency in male partners of RPL couples was significantly reduced (p= 0.03). The presence of KIR2DL3 in women with RPL was correlated with the presence of HLA-C2 alleles in their spouses (p= 0.03). Conclusion: Our data from a Turkish population suggest that male HLA-C2 homozygosity may play an important role in RPL. Additionally, an incidental match between male HLA-C2 and female HLA-C1 ligand KIR receptors might perturb the balance between activatory and inhibitory KIR-ligand interactions during pregnancy in couples affected by RPL. The roles of orphan KIR2DL5 and orphan KIR2DS3 in RPL remain obscure.

• Journal of Plant Biotechnology
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• v.49 no.4
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• pp.271-291
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• 2022

Pectin methylesterase (PME) plays an important role in vegetative and reproductive development and biotic/abiotic stress responses by regulating the degree of methyl-esterification of pectic polysaccharides in the plant cell wall. PMEs are encoded by a large multigene family in higher land plant genomes. In general, the expression of plant PME genes shows tissue- or cell-specific patterns and is induced by endogenous and exogenous stimuli. In this study, we identified PME multigene family members (CsPMEs) from the sweet orange genome and report detailed molecular characterization and expression profiling in different citrus tissues and two fruit developmental stages. We also discussed the possible functional roles of some CsPME genes by comparing them with the known functions of PMEs from other plant species. We identified 48 CsPME genes from the citrus genome. A phylogenetic tree analysis revealed that the identified CsPMEs were divided into two groups/types. Some CsPMEs showed very close phylogenetic relationships with the PMEs whose functions were formerly addressed in Arabidopsis, tomato, and maize. Expression profiling showed that some CsPME genes are highly or specifically expressed in the leaf, root, flower, or fruit. Based on the phylogenetic relationships and gene expression profiling results, we suggest that some CsPMEs could play functional roles in pollen development, pollen tube growth, cross incompatibility, root development, embryo/seed development, stomata movement, and biotic/abiotic stress responses. Our results shed light on the biological roles of individual CsPME isoforms and contribute to the search for genetic variations in citrus genetic resources.

• The Journal of the Korea Contents Association
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• v.21 no.2
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• pp.406-420
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• 2021

This study used Glaser's grounded theory method for the recognition of college students' majors in counseling psychology. Among the 3rd and 4th grade students majoring in counseling psychology, 19 students participated in the study, focusing on them for 5 months and collecting data through individual interviews. As a result of analyzing the data, 103 concepts, 26 subcategories, 9 upper categories, and 1 core category were derived. As a result of the study, first, the participants wanted to relieve the burden of people's hearts and had the hope of becoming a healer. Second, among the participants, in terms of their personality and aptitude for their major field, they perceived it as joy and satisfaction, such as 'It fits well with the major,' 'I made a good choice,' and 'It became a turning point in life.' However, they also recognized the incompatibility of the major, such as disharmony of aptitude, burden of the role of counselor, and rejection of major. Third, although participants showed usefulness in their major learning, their perception of the career path in their major was acting as anxiety and conflict, but their concerns and conflicts resulted in financial difficulties, poor job conditions, and emotional exhaustion caused by long-term study. The burden of job, fear of counseling job, limit of undergraduate graduation, uncertainty of career, etc. acted as an obstacle in choosing a major career. Fourth, as a result of analyzing the interview data of the participants, a core category called' understanding and growth and the will of the counselor to realize the dream' was derived. In conclusion, the participant's perception of their major is the change and growth of themselves, the formation of a healthy relationship with others, and satisfaction with the suitability of their major and their aptitudes. It turned out to have a lasting will to do.