• Proceedings of the Microbiological Society of Korea Conference
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• 2002.10a
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• pp.81-85
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• 2002

Oligotrophic bacteria isolated from forest soil showed a specific community consisting of various taxonomic groups compared with those in other soil or aquatic habitats. Based on the cell shape, the isolates were divided into four groups: regular rod, curved/spiral rod, irregular rod, and prosthecate bacteria. The cellular fatty acids 60 oligotrophic isolates were analyzed. At the dendrogram based on cellular fatty acid composition, four clusters(I-IV) were separated at a euclidian distance of about 50. Based on the 16S rDNA sequence analysis, the two representative strains(MH256 and MA828) of cluster 3 showed the close relation to genera, Xathomonas/Stenotrophomonas, but were not included in these genera. The isolates with Q-10 were also studied. They are corresponded to the two large groups in Proteobacteria alpha subdivision. One was incorporated in the genus Bradyrhizobium cluster, which also includes Agromonas, a genus for oligotrophic bacteria. The strains of the other group showed high similarity to the genus Agrobacterium. We attempted to screening of bioactive compounds from oligotrophs which was isolated from forest soil. The active compounds were analyzed by mass and NMR spectrum, one of them identified as crisamicin A. Another one designated as SAPH is a new compound. The results indicate that there were possibilities for finding new compounds from the rare microorganisms such as oligotrophs.

• Development and Reproduction
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• v.15 no.3
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• pp.239-247
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• 2011

Spermatogenesis and taxonomic values of mature sperm morphology of in male Septifer (Mytilisepta) virgatus were investigated by transmission electron microscope observations. The morphologies of the sperm nucleus and the acrosome of this species are the cylinder shape and cone shape, respectively. Spermatozoa are approximately 45-50 ${\mu}m$ in length including a sperm nucleus (about 1.26 ${\mu}m$ long), an acrosome (about 0.99 ${\mu}m$ long), and tail flagellum (about 45-47 ${\mu}m$). Several electron-dense proacrosomal vesicles become later the definitive acrosomal vesicle by the fusion of several Golgi-derived vesicles. The acrosome of this species has two regions of differing electron density: there is a thin, outer electron-dense opaque region (part) at the anterior end, behind which is a thicker, more electron-lucent region (part). In genus Septifer in Mytilidae, an axial rod does not find and also a mid-central line hole does not appear in the sperm nucleus. However, in genus Mytilus in Mytilidae, in subclass Pteriomorphia, an axial rod and a mid-central line hole appeared in the sperm nucleus. These morphological differences of the acrosome and sperm nucleus between the genuses Septifer and Mytilus can be used for phylogenetic and taxonomic analyses as a taxonomic key or a significant tool. The number of mitochondria in the midpiece of the sperm of this species are five, as seen in subclass Pteriomorphia.

• Nuclear Engineering and Technology
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• v.50 no.3
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• pp.504-511
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• 2018

In Lithuania, all spent nuclear fuel (SNF) resulted from the operation of the Ignalina Nuclear Power Plant (INPP), which had two Russian Acronym for "Channelized Large Power Reactor"-type reactors. After the final shutdown, the total amount of SNF at the INPP was approximately 22,000 fuel assemblies. All these assemblies will be stored for about 50 years and disposed of after that. The decision to shut down and decommission both reactors in Lithuania before termination of design period raises a significant challenge for the treatment of accumulated SNF. Therefore, various techniques and technologies for SNF management were developed and justified for that specific case, and a set of special equipment was installed at the INPP, the effectiveness of which was demonstrated during its operation. This article presents unique techniques related to the management of SNF adopted and commissioned at the INPP after its operation shutdown, namely fuel rod cladding leak tightness control system and special equipment for collection of possible spillage during handling of SNF assembly in the hot cell. The operational experience and measurement results of fuel rod cladding leak tightness control system are presented.

• Proceedings of the Korean Nuclear Society Conference
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• 1995.10a
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• pp.480-486
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• 1995

Turbulent flow field in a subchannel of bare rod bundles has been numerically simulated using the control volume based finite element method. Launder & Ying model of Reynolds stress and Lam & Bremhorst low-Reynolds number model are implemented in k-$\varepsilon$ equations and momentum equations. Secondary flows are simulated using the stream function and vorticity approach. The control volume based finite element method enable to use the upwind scheme (donor cell scheme). Sensitivity of the constants in the models are studied, and proper values are found to get the close result to the measured flow distributions.

• Korean Journal of Environmental Biology
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• v.18 no.4
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• pp.441-446
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• 2000

Trichoderma harzianum is an aggressive causal agent of green mold disease on mushroom cultivation. Some bacterial strains isolated, from oyster mushroom compost in Wonju, were found to have in vitro antifungal activity against Trichoderma harzianum ATCC 6385, 6504, and our isolates Trichoderma spp. Y and G. Further in vitro antifungal studies on several strains of phytopathogenic fungi showed that all of 12 phytopathogenic fungal strains were significantly inhibited by the isolated antifungal bacteria in Petri dishes. Of these, KATB 99121 showed the broadest inhibiting effect and displayed as negative coagulase, negative sulfide production and rod shape. KATB 99121 was resistant to ampicillin, chlorampenicol, and kanamycin. Identification of isolates was determined by Biolog GN system, and KATB 99121 was identified as Photobacterium logei because of 96 probability, 0.65 similarity, and 4.97 disturbance. With electron microscopy, thin section of KATB 99121 strain revealed typical rod-like shaped cell (0.6-0.8${\mu}{\textrm}{m}$$\times$1.5-2.0${\mu}{\textrm}{m}$) with prokaryotic structure and organization.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.36-36
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• 2003

Goldfish retina has been well studied to a great extent. In spite of that, electrical characteristics of dissociated horizontal cells(HCs) have not been identified in detail. Thus the cone-and the rod- HCs dissociated from goldfish retina were investigated electrophysiologically using whole-cell patch-clamping recording. To explore the basic electrical property, We examined voltage-dependent channels in all types of HCs. For the futher understanding of GABAergic pathway, the localization and distribution of GABA receptors was examined in cone- HCs including HC axon terminals(ATs).

• Tribology and Lubricants
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• v.36 no.3
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• pp.168-192
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• 2020

In recently designed diesel engines, the running conditions for piston-pin bearings have become severe because of the higher combustion pressure and increased temperature. Moreover, the metal removal from the bushing material has strongly reduced the ability of the antifriction material to accept asperity contacts. Therefore, it is necessary to find ways of reducing wear scar on the connecting-rod small-end bushing and piston-pin boss bearing related to the higher combustion pressure on the power cell of an engine. In this work, the position and level of material removal from the surfaces of the bushing and bearing under such severe operating conditions - for example, maximum power and torque conditions of a passenger car diesel engine - are estimated for several combinations of surface roughness. First, piston-pin rotating motion is investigated by calculating the friction coefficient at piston-pin bearings, the oil film thickness, and the frictional torques induced by hydrodynamic shear stress. Subsequently, the wear scarring on the surfaces of a connecting-rod small-end bushing and two piston-pin boss bearings related to piston-pin rotational motion is numerically calculated under the maximum power and torque operating conditions. This work is helpful to determine the reasonable surface roughness of the bushing and bearing for reducing wear volume occurring at the interface between a bearing and a shaft.

• Applied Microscopy
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• v.19 no.2
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• pp.119-137
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• 1989

It has been shown in this and earlier investigation that the turtle bladder mucosa has three main cell types on their mucosal surface. They are the granular cells, ${\alpha}$ CA cells, and ${\beta}$ CA cells. The three major transport mechanisms that occurs in the turtle bladder are sodium reabsorption, proton secretion, and bicarbonate secretion. In the present work the trans-port mechanisms by bladder epithelial cells of freshwater turtle, Pseudemys scripta, are summarized as follows. 1. The granular cells play an important role in sodium transport, while the ${\alpha}$ and ${\beta}$ CA cells do not appear to play a determining role in sodium transport. 2. It appears that the active sodium transport in the granular cells occurs in two-step process, implying that first, sodium diffuses into the cells, followed by an energy-dependent efflux step, which is catalyzed by the ouabain-sensitive Na-K ATPase. 3. The ${\alpha}$ type of CA cells are responsible for the proton secretion using the proton pump on the apical plasma membrane, while the ${\beta}$ type of CA cells are believed to be responsible for bicarbonate secretion. 4. When looked at under freeze-fracture electron microscopy, the apical plasma membrane of ${\alpha}$ cells have a characteristic population of rod-shaped intramembranous particles which are believed to be components of the proton pumps. Conversely, ${\beta}$ type of CA cells show rod-shaped particles in their basolateral plasma membranes, which is consistent with the proton absorptive, bicarbonate secretory mechanism. 5. In the turtle bladder, the ${\alpha}$ and ${\beta}$ type of cells are believed to be both responsible for proton transport, but in opposite directions.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.38 no.4
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• pp.443-449
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• 2014

To check the characteristics of nuclear fuels during an irradiation test, the nuclear fuel rod needs to be disassembled from the test rig located in the pool of the research reactor. Then, the disassembled fuel rod is delivered to the hot cell for intermediate examination. A fuel rod that passes the intermediate examination is delivered to the reactor pool to be reassembled into the test rig. The irradiation test is resumed with the reassembled test rig. Because nuclear fuel rods irradiated by neutrons are highly radioactive, all the disassembly and reassembly processes should be carried out in the pool of the research reactor to prevent operators being exposed to radiation. In particular, because a test rig is 5.4-m long and the reactor pool of HANARO is 6-m deep, special tools need to be developed for performing the disassembly and reassembly processes. In this study, a new assembly design of nuclear fuel rods for intermediate examination is introduced. Furthermore, tools for treating the irradiated fuel rod assembly are introduced, and their performance is verified by an out pile test.

• Biotechnology and Bioprocess Engineering:BBE
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• v.4 no.1
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• pp.12-16
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• 1999

Xenorhabdus nematophilus sp., an insect-pathogenic bacterium, was newly isolated from Korean entomopathogenic nematode of Steinernema carpocapsae, which can be used as a useful bioinsecticide. Primary and secondary form variants of Xenorhabdus nematophilus were observed when cultured in vitro. Primary form variants adsorbed bromothymol blue, while secondary form did not. However, many other characters of two variants were very similar. The variants were all rod-shaped and cell size was highly variable ranging from 0.5 by 2.0 ${\mu}$m to 1.0 by 5.0 ${\mu}$m. Both produced highly toxic substances and killed the insect larva within 20∼38 hr, indicating that insect pathogenicity of Xenorhabdus is not directly associated with its phase variation. In addition, cell-free culture supernatant of Xenorhabdus was sufficient to kill the insect larva by injecting it ito insect hemolymph; however, cell-harboring culture broth was more effective for killing the insect. The use of Xenorhabdus nematophilus may provide a potential alternative to Bacillus thuringiensis (Bt) toxins.