• 한국어병학회지
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• 제37권1호
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• pp.25-36
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• 2024

In this study, we analyzed the phylogenetic classification, epitope prediction, and pathogenicity of red sea bream iridovirus (RSIV) isolated from rock bream between 2019 and 2023. Phylogenetics based on genes encoding MCP and ATPase indicated that all five RSIV isolates belonged to RSIV subtype II. The deduced amino acid sequence of the MCP for the amplicons (1362 bp) obtained from RSIV isolates had a length of 453 amino acids. Among these, the amino acid sequences of the RSIV-19, 21, 22, and 23 isolates showed 100% identity, while the RSIV-20 isolate showed 99.78% identity with one residue difference at position 306. As a result of antigenicity analysis based on amino acid sequence, the antigenicity score of the RSIV-20 isolate was 0.6386 and the other RSIV isolates were 0.6365. Additionally, the prediction of their antigenic determinants resulted in a total of 17 identical antigenic plots. When each RSIV was inoculated into rock bream, no significant differences were observed with 100% cumulative mortality in all groups. This study provides data on the potential for genetic variation of RSIV isolated in the same marine area over the past five years, and the antigenicity and pathogenicity results of each isolate are expected to be useful information for selecting future vaccine strains.

• 한국해양생명과학회지
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• 제3권2호
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• pp.45-50
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• 2018

Rock bream iridovirus (RBIV) is a megalocytivirus widely infected in various fish species in Korea, causing symptoms of acute inflammation and enlargement of spleen. In our previous study, RBIV induced the initial upregulation but later down-regulation of proinflammatory cytokines and IFN1 gene expression. Signal transducers and activators of transcriptions (STAT) are transcription factors involved in the regulation of immune genes including IFNs. This study was conducted to analyse the expression of STAT2. The expressional study of STAT2 gene was performed in head kidney and spleen upon RBIV infection and immune stimulants like LPS or poly I:C in vitro. Consequently, STAT2 gene expression pattern was different in head kidney and spleen as it was significantly up-regulated by LPS from 4 h to 8 h but down-regulated at 24 h while up-regulated by poly I:C at 8 h in head kidney while, in spleen, STAT2 gene expression was down regulated by LPS but significantly up-regulated by poly I:C. Upon RBIV stimulation, STAT2 gene expression was significantly down-regulated by high dose RBIV at 4 h but up-regulated at 8 h and 24 h in head kidney. In spleen cells, it was up-regulated by medium dose RBIV at 4 h and by high dose RBIV at 4 h and 8 h but down regulated later then. In vivo, STAT2 gene expression was not significantly affected by RBIV infection while significant up-regulated by vaccination at day 7 post-vaccination, indicating STAT2 gene can be involved in adaptive immune response in rock bream.

• 한국응용과학기술학회지
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• 제14권3호
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• pp.29-38
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• 1997

Low toxic concentrated oil dispersant using n-Paraffin and Di(ethylene glycol)mono butylether mixed solvent was prepared, and tested by oil dispersant performance test method, and oil dispersant efficiency was measured using vertical shaking method to 3 kinds of Crude oil, Bunker oil and W/O emulsions with different physical properties by appling the prepared dispersant. Although toxicity test was performed with Flat fish and Rock fish by appling the mixed oils emulsified using prepared oil dispersant, couldn't find the toxicity to them. Concentrated oil dispersant prepared has a good dispersion efficiency of 97.2% after 0.5min settling time and 28.3% after 10min settling time to Bunker B oil with 10% water solution. Especially, the concentrated oil dispersant showing the low toxicity to Oryzias Latipes(24hr, TLm) was 54,000 ppm and to Brine Shrimp Artemia(24hr, TLm) was 51,000ppm, and also, it was completely biodegradated to 99.1% after $7{\sim}8$days.

• 자원환경지질
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• 제37권1호
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• pp.133-142
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• 2004

본 연구의 목적은 실내실험을 실시하여 석회석 및 인회석의 ARD 내의 비소제거 효율성을 평가하고, 인회석 및 생선뼈를 이용하여 AMD내의 중금속 제거 효율성을 평가하는 것이다. 실내실험 결과, 석회석과 인회석의 pH, 비소제거율은 유속에 반비례하였으며, ARD 처리에 있어 유속 0.6$m{ell}$/min/kg에서 석회석의 비소제거율은 37%인 반면 인회석은 유속 0.6$m{ell}$/min/kg에서 비소를 100% 제거하였다. 인회석의 용해율은 석회석보다 25배 정도 높았다. AMD 처리에 있어 생선뼈의 용해율은 인회석 보다 높았으며, 유출수의 pH도 생선뼈의 경우가 인회석 보다 더 높게 나타났다. 중금속 제거율은 비소제거율을 제외하면 생선뼈가 인회석 보다 높았다. AMD와 인회석의 반응으로 생긴 침전물은 유기 슬러지 형태를 보이나, 인회석과 반응하여 생성된 침전물의 경우 무기질 입자로서 유기 슬러지에 비해 쉽게 침전되며 침전 된 후 석고에 의해 교결된다. 결론적으로 인회석은 광범위한 pH영역에서 광산배수의 침전제로 사용될 수 있고, 생선뼈는 고농도로 오염된 AMD에 사용할 수 있다.

• 한국어병학회지
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• 제19권3호
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• pp.253-265
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• 2006

본 연구는 동물용 생화학 분석장치를 이용하여 분석한 어류 혈액검사 항목 가운데 적용할 수 있는 분석항목의 활용도를 평가함과 동시에 어류 혈액학적 건강진단에 필요한 분석항목의 참고자료를 얻고자 하였다. 경남 지역의 해상가두리 어류 양식장에서 사육중인 육안적으로 보아 건강한 조피볼락 (Sebastes schlegeli), 참돔 (Pagrus major), 돌돔 (Oplegnathus fasciatu) 및 감성돔 (Acanthopagrus schlegeli) 총 522마리를 대상으로 hematocrit 와 간편하게 혈액성분을 측정할 수 있는 FUJI DRI-CHEM 3000 기종을 이용하여 혈장화학적 성분 분석을 실시하였다. Hematocrit (Ht)는 microhematocrit법에 의해 그리고 hemoglobin (Hb), 총단백 (TP), 알부민 (ALB), 알칼리성포스파타제 (ALP), 요소질소 (BUN), 젖산탈수소효소 (LDH), 중성지방 (TG), 총콜레스테롤 (TCHO), 크레아티닌 (CRE), aspartate aminotransferase (AST), alanine aminotransferase (ALT), 포도당(GLU)은 정상범위의 표준자료가 이미 프로그램화되어 장치된 동물용 생화학 분석장치인 FUJI DRI-CHEM 3000 기종을 이용하여 분석하였다. 그 결과, 측정된 Hb와 혈장화학성분의 실제 값들은 본 기종의 하한 및 상한 한계치를 상당히 벗어났다. 본 장비로부터 분석값을 검출하지 못하였던 비율을 살펴보면, ALB 및 ALT는 68～66%, LDH, TP, ALP 및 GLU는 42～21%, TCHO, AST, TG, Hb 및 CRE는 18～3%의 범위를 나타내었으나, BUN의 분석값은 모두 검출한계를 벗어나지 않았다.

• Geomechanics and Engineering
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• 제14권6호
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• pp.545-552
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• 2018

Blast-induced ground vibrations by a significant amount of explosives may cause many problems for mining slope stability. Geological discontinuities have a significant influence on the transmission of dynamic pressure of detonation and according to their position relative to the slope face may have damaging or useful impacts on the slope stability. In this study, the effect of geological discontinuities was investigated by modelling a slope with geological discontinuities through applying the dynamic pressure in three-dimensional discrete element code (3DEC). The geological discontinuities in four states that generally apperceived in mine slopes are considered. Given the advantages of the pressure decay function defined by some researcher, this type of function was used to develop the pressure-time profile. The peak particle velocities (PPV) values were monitored along an axis by utilization of Fish programming language and the results were used as an indicator to measure the effects. As shown in the discontinuity-free model, PPV empirical models are reliable in rocks lacking discontinuities or tightly jointed rock masses. According to the other results, the empirical models cannot be used for the case where the rock mass contains discontinuities with any direction or dip. With regard to PPVs, when the direction of discontinuities is opposite to that of the slope face, the dynamic pressure of detonation is significantly damped toward the slope direction at the surface of discontinuities. On the other hand, when the discontinuities are horizontal, the dynamic pressure of detonation affects the rock mass to a large distance.

• 한국소음진동공학회논문집
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• 제19권3호
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• pp.313-319
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• 2009

The blasting has a lot of economic efficiency and speediness but it can damage to a neighbor structure, a domestic animal and a cultured fish due to the blasting vibration, then the public grievance is increased. Therefore, we need to manage the blasting vibration efficiently. The prediction of the correct vibration velocity is not easy because there are lots of different kinds of the scale of blasting vibration and it has a number of a variable effect. So we figure the optimum line through the least-squares regression by using the vibration data measured in hard rock blasting and compared with the design vibration prediction equation. As a result, we confirm that the vibration estimated in this paper is bigger than the design vibration prediction equation in the same charge and distance. If there is a Gaussian normal distribution data on the left-right side of the least squares regression, then we can estimate the vibration prediction equation on reliability 50%(${\beta}=0$), 90%(${\beta}=1.28$), 95%(${\beta}=1.64$). 99.9%(${\beta}=3.09$). As a result, it appears to be suitable that the reliability is 99% at the transverse component, the reliability 95% is at the vertical component, the reliability 90% is at the longitudinal component and the reliability is 95% at the peak vector sum component.

• 한국환경생태학회지
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• 제25권1호
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• pp.71-80
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• 2011

2009년 8월부터 2010년 4월까지 탄천 9개 미소서식지에서 어류상과 피라미 개체군의 특성을 조사하였다. 조사 기간동안 채집된 어류는 총 7과 17종개체가 확인되었다. 출현한 어종 중 한국고유종은 Hemiculter eigenmanni와 Odontobutis interrupta로 2종(11.8%)이었으며 국외도입종은 Microterus salmoides 1종 이었다. 우점종으로는 Zacco platypus, 아우점종으로는 Carassius auratus 이었다. 서식처별로는 댐형 웅덩이, 사행형 웅덩이, 평여울, 거석형 웅덩이 등에서 Carassius auratus가 우점하였고, Pseudogobio esocinus는 샛강, Rhinogobius brunneus는 급여울, Z. platypus 는 개방형 하도습지, 낙차형 웅덩이, 폐쇄형 웅덩이 등에서 우점하였다. Z. platypus의 성장도와 비만도의 회귀계수 b값이 3.29의 값으로 나타났고 PCA 분석 결과 탄천에서 크게 3개의 Group으로 나누어졌다. 탄천의 서식처 별 Bray-Curtis Cluster 분석을 실시한 결과 낙차형 웅덩이와 폐쇄형 하도습지가 65.9%로 가장 유사한 것으로 나타났으며, 30.4%로 댐형 웅덩이와 급여울이 가장 대조적인 것으로 확인되었다.

• 한국수산과학회지
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• 제42권4호
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• pp.322-328
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• 2009

Three-hundred and sixteen Escherichia coli strains from seawater, and a variety of farmed fishes, including oliver flounder (Paralichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagrus major) and sea bass (Lateolabrax japonicus) between May to October in 2004, were tested by agar dilution method to determine their susceptibility patterns to 17 antimicrobial agents. Overall, 92.1% of Escherichia coli isolates from samples showed antimicrobial resistance to at least one antimicrobial agent and the multiple resistance was seen in 173 isolates (54.7%). The resistance of E. coli isolates to tetracycline (74.1%) was highest, followed by cephalothin (69.9%), doxycycline (66.5%), streptomycin (47.2%), ampicillin (46.2%), cefazolin (31.6%), enrofloxacin (31.0%). norfloxacin (28.2%). The most frequent resistance pattern was TE-D-CF-CIP-ENO-NOR-AM-S-C-SXT-AmC-CZ (14.7%), followed by CF (6.2%), TE (5.1%), TE-CF (4.5%) in 177 isolates from fishes and TE-D-CF (7.2%) followed by TE-D-CF-S (5.8%), CF and TE-D-S (3.6%) in 139 isolates from seawater.

• 수산해양기술연구
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• 제55권2호
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• pp.129-137
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• 2019

This study was conducted to investigate the effects of underwater noise caused by pile driving during marine construction on fish. In this study, the three gray rockfish were released about 1 km away from the construction site of wind power generation on July 18, 2018 and traced using two acoustic telemetry techniques. The behavior of the fish was analyzed by calculating the moving distance, swimming speed and direction of the gray rockfish. In the results of the acoustic tracking using the ship, the rockfish moved about 2.11 km for about two hours at a speed of $0.28{\pm}0.14m/s$ (0.94 TL/s). The bottom depth of the trajectory of the rockfish was $1.0{\pm}0.6m$ on average. There was a significant directionality in swimming direction of the gray rockfish, and there was no significant correlation between the swimming direction and tidal current direction. Moving distance during 5 minutes (5MD) during pile driving and finishing operations between rock surface and bedrock were 0.94-0.96 times (76.0-77.0 m) and 1.81-2.73 times (146.0-219.5 m), respectively, compared with no pile driving. This study is expected to be used as a basic data of fish behavior research on underwater noise.