• Journal of Microbiology
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• v.44 no.6
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• pp.671-673
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• 2006

A new 4.87 kb Escherichia-Pseudomonas shuttle vector has been constructed by inserting a 1.27 kb DNA fragment with a replication origin of a Pseudomonas plasmid pRO1614 into the 3.6 kb E. coli plasmid pBGS18. This vector, designated pJH1, contains an aminogly-coside phosphotransferase gene (aph) from Tn903, a lacZ' gene for $\alpha$-complementation and a versatile multiple cloning site possessing unique restriction sites for EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, BspMI, PstI, SphI, and HindIII. When pJH1 was transformed into E. coli DHS${\alpha}$ and into P. putida HK-6, it was episomally and stably maintained in both strains. In addition, the enhanced green fluorescent protein (EGFP) gene which was transcriptionally cloned into pJH1 rendered E. coli cells fluorescence when its transformants were illuminated at 488 nm.

• Journal of the Optical Society of Korea
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• v.15 no.2
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• pp.182-186
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• 2011

A poling method using multiple negative voltage was introduced to fabricate periodically poled lithium niobate (PPLN) devices with quasi-phase matching (QPM) period of $12.9\;{\mu}m$ by utilizing an real-time visualization system. We also performed variation of the electric field during the poling. Two different conventionally used poling method, negative and positive single pulses, were used and the poling quality compared through microscopic images and far-field diffraction pattern analysis. Etched images on the +z and -z surfaces of PPLN showed that negative multiple pulse poling presented the highest periodicity in domain structures among the three methods. Duty ratio and its standard deviation were measured by analyzing far-field diffraction patterns. The newly introduced method of negative multiple pulse poling had duty ratio of 0.42 which was close to the ideal value of 0.50 and standard deviation of 0.020 that was about 3 times smaller than that of the other conventional methods.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.34 no.6
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• pp.1765-1777
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• 2014

This study evaluated PMM (Probability Matching Method) for parameter estimation of the Z - R relation. As a first step, the sensitivity analysis was done to decide the threshold number of data pairs and the data interval for the development of a histogram. As a result, it was found that at least 1,000 number of data pairs are required to apply the PMM for the parameter estimation. This amount of data is similar to that collected for two hours. Also, the number of intervals for the histogram was found to be at least 100. Additionally, it was found that the matching the first-order moment is better than the cumulative probability, and that the data pairs comprising 30 to 100% are better for the PMM application. Finally, above findings were applied to a real rainfall event observed by the Bislsan radar and optimal parameters were estimated. The radar rain rate derived by applying these parameters was found to be well matched to the rain gauge rain rate.

• Proceedings of the KOSOMBE Conference
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• v.1994 no.05
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• pp.9-15
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• 1994

In last few decades, medical imaging techniques have been developed startling progress. Especially in MRI (Magnetic Resonance Imaging), many imaging techniques such as chemical shift imaging, flow imaging, diffusion and perfusion imaging, fast imaging, susceptibility imaging and functional imaging have been studied and many of them were well known as useful diagnostic instruments. In this paper, recently developing techniques, i.e., NMR microscopy, fringe field imaging and functional imaging will be presented.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2010.04a
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• pp.473-474
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• 2010

• Proceedings of the KOSOMBE Conference
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• v.1995 no.11
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• pp.124-127
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• 1995

Due to the self-diffusion of nuclear spins, the edge of phantoms is enhanced in the magnetic resonance imaging (MRI), especially in the case of microscopy [1]. According to several published works, theory has been established that the edge enhancement is caused by the motion narrowing around bounded regions due to diffusions of nuclear spins during data acquisition. It is found, however, that the signal decreases due to the diffusion attenuation and image is distorted as edge of the image is sharpened. In this paper, we wilt investigate this signal loss during data acquisition and its effects on image, i.e., image edge enhancement due to the diffusion phenomenon. This result is new and different from the previously discussed edge enhancement due to the diffusion, namely, by motion narrowing effect or spin bouncing effect at the boundary.

• Proceedings of the KOSOMBE Conference
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• v.1995 no.11
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• pp.167-170
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• 1995

An frequency modulated (FM) DANTE pulse sequence generates a quadratic phase toward the transverse of image by an FM RF pulse. In the image of a serious susceptibility effect, the phase due to the difference of the susceptibility in the pixel occurs susceptibility error which arise signal loss. But the signal loss due to the susceptibility effect in the pixel is reduced when the quadratic phase adds in the pixel. In this paper, we have generated a quadratic function toward the transverse (X-Y) using FM DANTE sequence and the susceptibility effect is reduced in the gradient echo (GE) imaging. Computer simulation and experimental results is obtained by using a whole-body KAIS 2.0T NMR system.

• Proceedings of the KOSOMBE Conference
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• v.1995 no.11
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• pp.173-176
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• 1995

We have developed a fast steady state free precession interferometry (SSFPI) technique which is useful for the fMRI (functional Magnetic Resonance Imaging). As is known, SSFP sequence with a suitable adjustment of gradient (readout) allows us to measure precession angle $\theta$ which is in turn related to the field inhomogeneity [1-3]. When the method is applied to the susceptibility effect based functional magnetic resonance imaging (fMRI), it was found that the direct susceptibility effect measurement was possible without perturbations such as the backgrounds and inflow effect. In this paper, simulation results and experimental results obtained with 2.0 Tesla MRI system are also presented.

• Proceedings of the KOSOMBE Conference
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• v.1990 no.11
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• pp.19-23
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• 1990

A new technique for reducing the susceptibility artifact in gradient echo imaging which uses a tailored RF pulse is proposed. It is applied to the case of imaging where artifacts and distortions arise due to the high local magnetic field inhomogeneity i. e., the susceptibility. The signal loss and void phenomena due to susceptibility in a voxel are studied and a correction method using a tailored RF pulse is proposed. Applications of this method in imaging are given and experimental results obtained using an human volunteer with a 2.0 T KAIS NMR system are presented.

• Proceedings of the KOSOMBE Conference
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• v.1992 no.05
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• pp.92-97
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• 1992

The pulsatile nature of blood flow makes artefacts in 2D Fourier transform image. Spatial presaturation is known to be effective in eliminating flow artefacts when the spin echo acquisition is employed. However. this method requires additional RF pulse and spoiling gradient for presaturation. In this paper a new flow saturation technique which does not require additional saturation-RF and gradient is proposed. The proposed technique is equivalent to the existing saturation technique but the elimination of the flow component is achieved by a pair of tailored $90^{\circ}-180^{\circ}$ RF pulses in tile spin echo sequence. By use of two tailored RF pulses with opposite phase polarity, a linear phase gradient is generated for those moving materials and consequently all the spins of moving materials become dephased thereby no signal is observable. Computer simulations and experimental results obtained using both a phantom and a human volunteer with a 2.0 T whole body system are also presented.