• Proceedings of the Ginseng society Conference
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• 2002.10a
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• pp.376-384
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• 2002

Object To find out which of the 27 ginsenosides isolated from Panax ginseng C.A. Mey that may inhibit the proliferation of human osteosaocoma cell line $U_2OS$. Methods Effects of each individual ginsenoside on the proliferation of $U_2OS$ cell were studied by determining the viability of cancer cells during culture with or without the presence of the test compound. DNA assay was determined by flow cytometry. Results Ginsonosides -Ro, $-Rh_l,\;-Rh_2,\;-F_1\;and\;-L_8$ at concentrations of 5 ,umol/L could obviously suppress the proliferation of $U_2OS$ cells while ginsenosides $-Rg_1,\;-F_3,$ -Rf, PPT and PT significantly inhibited the cancer cells. Flow cytometry revealed that ginsenosides $-Ro,-Rg_1-Rf,-F_1-Rh_2,PPT$ and PT induced cell cycle arrest at $G_0/G_1$ phase with obvious decrease of cell count at Sand $G_2+M$ phase, Moreover, ginsenosides $-Rf_1,-Rg_1,\;-F_1$ and PPT induced significantly high rates of cell death as compared with the control. Conclusion These data suggested that ginsenosides inhibited $U_2OS$ proliferation Via cell cycle arrest or induction of cell death.

• Molecules and Cells
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• v.23 no.2
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• pp.161-169
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• 2007

We identified two alternatively spliced variants of the peroxisomal targeting signal 1 (PTS1) receptor protein Pex5ps in monocot (rice, wheat, and barley) but not in dicot (Arabidopsis and tobacco) plants. We characterized the molecular and functional differences between the rice (Oryza sativa) Pex5 splicing variants OsPex5pL and OsPex5pS. There is only a single-copy of OsPEX5 in the rice genome and RT-PCR analysis points to alternative splicing of the transcripts. Putative light-responsive cis-elements were identified in the 5' region flanking OsPEX5L and Northern blot analysis demonstrated that this region affected light-dependent expression of OsPEX5 transcription. Using the pex5-deficient yeast mutant Scpex5, we showed that OsPex5pL and OsPex5pS are able to restore translocation of a model PTS1 protein (GFP-SKL) into peroxisomes. OsPex5pL and OsPex5pS formed homo-complexes via specific interaction domains, and interacted with each other and OsPex14p to form hetero-complexes. Although overexpression of OsPex5pL in the Arabidopsis pex5 mutant (Atpex5) rescued the mutant phenotype, overexpression of OsPex5pS only resulted in partial recovery.

• Journal of the Korean Institute of Landscape Architecture
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• v.28 no.1
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• pp.136-146
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• 2000

Although the urban elevated road is welcomed in modern society to deal with urban traffic problems, its negative influence on both the pedestrian environment and urban landscape has been frequently observed. Furthermore, the space under elevated structures has been ignored, being the lost space. Recently, several efforts have been devoted into bringing back the lostspace. However, any specific design guidelines shown in the street design of Japan and Singapore have not been suggested in Korea, yet. This study proposes a case of the Nengdong-Ro street design where the two-story urban elevated road is being constructed and negative effects of the elevated road are largely expected. One of the purposes of Nengdong-Ro street design proposed in this study is to relieve the negative effect os the two-story elevated road, and to provide a better pedestrian environment in Nengdong-Ro. The other purpose is to suggest general guidelines that can be applied to the similar context as Nengdong-Ro. It is considered that the space under the elevated road generally consist of three sections: 1) main section where the elevated road runs parallel with the ground, 2) landing section where elevated road goes down to the ground, and 3) facility section where facilities such as the platform and the ticketing booth are located. The design guidelines are suggested for each section, because each section has a different situation. Plans, section and elevations and the details of the street furniture are also incorporated to support the design guidelines.

• The KIPS Transactions:PartD
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• v.10D no.5
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• pp.773-780
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• 2003

SecuROS(Secure & Reliable Operating System) prevents and blocks possible system cracking by implementing additional security functions in FreeBSD 4.3 operating system (OS) kernel, including access control, user authentication, audit trail, encryption file system and trusted channel. This paper describes access control technique, which is one of core technologies of SecuROS, introduces the implementations of DAC, MAC and RBAC, all of which are corresponding access control policies, and show security and results of performance measurement on the basis of application of access control policies. Finally, security and performance between conventional OS environment and environment adopting access control policy is described.

• Proceedings of the KIEE Conference
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• 1996.07c
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• pp.1851-1853
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• 1996

In this paper the criteria for insulating oil condition of pole transformer were suggested by applying the UV-Visible spectrophotometric method. Ageing of insulating oil caused the change in absorbances of oil os well os the deterioration of electrical properties of oil. By comparing the electrical properties such as $tan{\delta}$ and breakdown voltage with the UV-Visible absorbances of oil, criteria for absorbance values determining oil condition were established. This UV-Visible spectrophotometric method might be applied in ageing analysis of oil and be expected to replace the current method.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.4
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• pp.1487-1494
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• 2015

Background: To investigate the clinical efficacy of expanded activated autologous lymphocytes (EAAL) in patients with small cell lung cancer (SCLC). Materials and Methods: A total of 32 SCLC patients were selected and randomly divided into EAAL treatment and control groups, 16 cases in each. EAAL were obtained by proliferation of peripheral blood mononuclear cells (PBMCs) of patients followed by phenotype determination. Clinical data of all patients were recorded. Patients of both groups were followed up and the overall survival (OS) were compared retrospectively. Results: After culture and proliferation in vitro, the percentages of $CD3^+$, $CD3^+CD8^+$, $CD45RO^+$, $CD28^+$, $CD29^+$, $CD8^+CD28^+$ and $CD3^+CD16^+/CD56^+$ cells increased markedly (p<0.05). The OS of the EAAL treatment group was longer than that of control group, but the difference was not statistically significant (p=0.060, HR=0.487, 95%CI 0.228~1.037). 1- to 3-year survival rates in EAAL treatment group were longer than those in control group, but there was still no significant difference (p>0.05). COX multivariate regression analysis showed that the number of chemotherapy cycles and the application of EAAL immunotherapy were independent prognostic factors for SCLC patients. The OS in females and chemotherapy${\leq}6$ cycles were obviously prolonged after EAAL immunotherapy. Conclusions: In vitro induction and proliferation of EAAL is easy and biologically safe. Generally, EAAL adoptive immunotherapy can evidently prolong the OS of SCLC patients.

• Journal of Life Science
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• v.20 no.2
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• pp.281-291
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• 2010

We investigated the antioxidant effects of hederagenin 3-O-b-D-glucopyranosyl($1{\rightarrow}3$)-a-L-rhamnopyranosyl($1{\rightarrow}2$)-a-L-arabinopyranoside (HDL) isolated from root bark of Ulmus davidiana on the activity of enzymes related to reactive oxygen species (ROS) in human osteosarcoma U2OS cells. Cobalt chloride ($CoCl_2$), a transition metal, was used as an inducer of oxidative stress, generating hydrogen peroxide ($H_2O_2$) via increasing xanthine oxidase (XO) activity. The increased levels of $H_2O_2$, XO, ferritin, and ferritin iron by $CoCl_2$ were diminished effectively by co-treatment with HDL in U2OS cells. Furthermore, decreased levels of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) by $CoCl_2$ were highly increased by co-treatment with HDL in U2OS cells; however, the levels of glutathione peroxidase (GPx) did not change. The increased contents of TBARS related to lipid peroxidation were significantly reduced by HDL in U2OS cells. The concentration of GSH changed in a pattern that went against regulated TBARS by $CoCl_2$ and HDL. We examined the expression of p53, $p21^{CIP1/WAF1}$, and $p27^{KIP1}$ proteins related to oxidative stress and cell cycle regulation. As a result, the expression of $p27^{KIP1}$ modulated by $CoCl_2$ was not changed by HDL. However, the expression of p53 and $p21^{CIP1/WAF}$ increased by $CoCl_2$ was reduced by HDL in U2OS cells. Together with alteration of p53 and $p21^{CIP1/WAF1}$ proteins, the accumulated cells at G1 phase by $CoCl_2$ was decreased by HDL in U2OS cells. Our data suggests that HDL inhibits $CoCl_2$-generated ROS in U2OS cells, providing potentially new antioxidant compounds that are isolated from natural products.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.5B
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• pp.449-454
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• 2009

In this paper, we analyze the hardware and software platform for constructing the USN in the vessel environment. Specifically, we analyze the mote technology based on the CC2420 in the hardware platform and analyze the TinyOS platform in the software platform. We also analyze the physical layer of IEEE802.15.4 which is the standard of the USN communication. In the simulation, we evaluate the performance in the physical layer of ZigBee/IEEE 802.15.4 by using the MATLAB and verify the validity of constructing the USN in the vessel environment based on the simulation results.

• Journal of the Korean Association of Geographic Information Studies
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• v.15 no.2
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• pp.46-56
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• 2012

Recently, when software architecture is designed, the efficiency of reusability is emphasized. The reusability of the design can raise the quality of GIS software, and reduce the cost of maintenance. Because the object oriented GoF design pattern provides the class hierarchy that can represent repetitively, the importance is emphasized more. This method that designs the GIS software can be applied from various application systems. A multiple distributed sensor network system is composed of the complex structure that each node of the sensor network nodes has different functions and sensor nodes and server are designed by the combination of many classes. Furthermore, this sensor network system may be changed into more complex systems according to a particular purpose of software designer. This paper will design the CATL model by applying Facade pattern that can enhance the efficiency of reuse according to attributes and behaviors in classes in order to implement the complicated structure of the multiple distributed sensor network system based on TinyOS. Therefore, our object oriented GIS design pattern model will be expected to utilize efficiently for design, update, or maintenance, etc. of new systems by packing up attributes and behaviors of classes at complex sensor network systems.

• Proceedings of the Korea Information Processing Society Conference
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• 2017.04a
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• pp.40-43
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• 2017

OS-level 가상화 기술은 애플리케이션을 배포하기 위한 새로운 패러다임으로서, 가상 머신을 대체할 수 있는 기술이다. 특히 컨테이너는 기존의 리눅스 컨테이너에 유니온 마운트 포인트(Union Mount Point)와 레이어 구조의 이미지를 적용함으로써 보다 빠르고 효율적인 애플리케이션의 배포가 가능하다. 이러한 컨테이너의 특징들은 RoW(Redirect-on-Write), CoW(Copy-on-Write) 등의 스냅숏 기능을 제공하는 특정 파일 시스템에서만 사용될 수 있으며, 어플리케이션의 특징에 따라 적절한 파일 시스템을 사용해야한다. 따라서 본 논문에서는 컨테이너 이미지의 레이어구조를 사용할 수 있는 파일 시스템들의 특징을 설명하고 이에 따른 쓰기 작업의 성능 평가를 진행한다.