• BMB Reports
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• v.40 no.1
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• pp.22-28
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• 2007

MyoD, expressed in skeletal muscle lineages of vertebrate embryo, is one of muscle-specific basic helix-loop-helix (bHLH) transcription factors, which plays a key role in the determination and differentiation of all skeletal muscle lineages. In this study, a cDNA of grass carp MyoD was cloned and characterized from total RNA of grass carp embryos by RT-PCR. The full-length cDNA of grass carp MyoD is 1597 bp. The cDNA sequence analysis reveals an open reading frame of 825 bp coding for a protein of 275 amino acids, which includes a bHLH domain composed of basic domain (1-84th amino acids) and HLH domain (98-142th amino acids), without signal peptide. Then the MyoD cDNA of grass carp was cloned to yeast expression vector pPICZ$\alpha$A and transformed into P. pastoris GS115 strain, the recombinant MyoD protein with a molecular weight of about 31KD was obtained after inducing for 2d with 0.5% methanol in pH 8.0 BMGY medium, and the maximum yield was about 250 mg/L in shaking-flask fermentation. The results were expected to benefit for further studies on the crystal structure and physiological function of fish MyoD.

• Water Engineering Research
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• v.6 no.1
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• pp.17-30
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• 2005

The bed evolution of the stretch of the River Rhine between km-812.5 and km-821.5 is characterised by general bed degradation as a result of the river training works and dredging activities of the last two centuries. The degradation of the river bed affects the water levels, and so the navigation conditions. To combat the erosion of the river bed with the aim to keep up the shipping traffic and to avoid the ecological system damages due to water level reductions, sand-gravel-mixtures were added to the river (so called artificial grain feeding activities). This paper presents the results of an application of a graded sediment transport model in order to study morpholodynamical characteristics due to artificial grain feeding activities in the river stretch. The finite element code TELEMAC2D was used for flow calculation by solving the 2D shallow water equation on non-structured grids. The sediment transport module SISYPHE has been developed for graded sediment transport using a multiple layer model. The needs to apply such graded sediment transport approaches to study morphological processes in the domain are discussed. The calculations have been carried out for the case of middle water flow and different size-fraction distributions. The results show that the grain feeding process could be well simulated by the model.

• International Journal of Contents
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• v.8 no.3
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• pp.34-41
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• 2012

The concept of vegetation freeboard equivalence (VFE) is presented from the comparison between the rise in stage with/without vegetation and the freeboard height under design discharge conditions. In South Korea, the freeboard height of large, medium and small rivers is defined as a function of river discharge. Two models are used for this analysis of flood stage with and without vegetation: the 1-D model HEC-RAS and the 2-D model RMA-2. Both models are applied to three river study sites of the Geum River in South Korea as representative sites for a large, a medium and a small river. The analysis shows that without vegetation, both models provide comparable results and the calculated results are in very good agreement with the design configuration. The vegetation effects on the medium river are less significant, and the freeboard is adequate to contain the rise in stage from the added floodplain vegetation in large rivers. The concept of vegetation freeboard equivalence is therefore useful for the analysis of flood river stages after the restoration of channels with increased floodplain vegetation.

• Journal of the Korean Society of Hazard Mitigation
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• v.8 no.1
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• pp.117-123
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• 2008

In this study, PHABSIM which is a sample for 1D physical habitat and River2D, which is a sample for 2D physical habitat were applied to the main streams of Han River in order to calculate an optimum flow considering the habitats of fishes in determining the instream flow. Moreover, the Weighted Usable Area (WUA) of the two samples in each growth step (adults and spawning) of the target fish type was compared and reviewed. The optimal flow value was calculated by considering the conditions for inhabiting fishes. As a result of the correlation analysis for WUA from 1D and 2D samples was 0.87 to 0.99. The optimum flow considering the conditions of inhabiting fishes showed insignificant difference of $3m^3/s\;to\;5m^3/s$ with the exception of adults in Moon-Mak and spawning in Dal-Chun.

• Proceedings of the Korea Contents Association Conference
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• 2009.05a
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• pp.437-441
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• 2009

The modern times is as special as from the river, the river is very important of our life. The importance to preserve the river environment has been issued and the river developing method is being changed to use potential function of nature as well as flood control. Essential element of the river restoration is a vegetation. The flow resistance by vegetation along the river banks is greatly increase the flood stage. Therefore, the flow resistance due to vegetation in the river and roughness coefficient changes to understand the hydraulic characteristics is an important elements in the river restoration. The purpose of this study is to analyze the flood stage and the aspects of riverbed changes due to the corridor restoration with river vegetation. In order to simulate the flood stage and riverbed changes, HEC-RAS, RMA-2, and SED-2D model were applied for the upstream and downstream in study reaches, respectively.

• Journal of Environmental Health Sciences
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• v.26 no.4
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• pp.97-104
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• 2000

The purpose of this is made an examination of phosphorus loading model for eutrophication response in the Yongsan lake. For the model, we measured the total amount of nutrients derived from the Yongsan river watershed, inflow rate to the Yongsan lake, water quality, and water budget from January to December in 1999. The total amount of precipitation in the Yongsan river watershed was 4,951.7$\times$10$^{6}$ ㎥/y and inflow amount was 2,569.7$\times$10$^{6}$ ㎥/y, therefore the outflow rate of the Yongsan river watershed was 51.9%. The develop loading of total nitrogen was 86,928.1kg/d and that of total phosphorus was 22,007.6kg/d at the Yongsan river watershed, But, as the inflow loading of total nitrogen was 33,962kg/d and the inflow loading of total phosphorus was 2,218kg/d to the Yongsan lake. so each infolw rate was 39.0% and 10.1%. The hydraulic residence time was 34days, total phosphorus loading [L(P)] on the surface area was 23.398g/㎥/y, the hydraulic load( $Q_{s}$) of inflow water was 74.269m/y, the reserve rate of phosphorus in the lake was 0.359, and the settinh velocity of phosphorus was 0.114m/d at the Yongsan lake. Mathematical model of phosphorus loading to estimate the responses of eutrophication at the Yongsan lake is [ $P_{j}$] = 0.838 [L(P)/Q.(1+√ $T_{w}$)$^{-1}$ ] . ] . .

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.2
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• pp.1441-1449
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• 2015

At the bends of a river, the deflected flow of high energy erodes the embankment and transports sediment, ultimately changing the river's course. Such phenomena lead to instability of the embankment and may cause serious problems for nearby roads, land, or riverside structures. This study intends to analyze the characteristics of hydraulic flow at S-shaped bends after the installation of Riprap Weirs designed to prevent changes in a river's course and embankment erosion. An experimental channel was constructed by a two-dimensional CCHE2D model, and analyzed the characteristics of hydraulic flow with and without the Riprap Weirs. Also, the characteristics of hydraulic flow at the inner and outer sides of river bends and at points near reservoirs were analyzed, depending on the intervals between the Riprap Weirs.

• Journal of Korean Society on Water Environment
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• v.37 no.3
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• pp.217-228
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• 2021

This paper discusses a methodology where an integrated modelling framework is used to quantify the risk derived from anthropic activities on habitats and species. To achieve this purpose, a tool comprising the Delft3D and HABITAT model, was applied in the Yeongsan river. Delft3D effectively simulated the operational condition and flow of weirs in river. In accuracy evaluation of the Delft3D-FLOW, the Bias, Pbias, Mean Absolute Error (MAE), Nash-Sutcliffe Efficiency (NSE), and Index of Agreement (IOA) were used, and the result was evaluated as grade above 'Satisfactory'. The HABITAT calculated Habitat Suitability Value (HSV) for the following eight species: mammal, fish, aquatic plant, and benthic macroinvertebrate. An Area was defined as a suitable habitat if the HSV was larger than 0.5. HABITAT was judged accurately by measuring the Correct Classification rate (CCR) and the area under the ROC curve (AUC). For benthic macroinvertebrate, the CCR and AUC were 77% and 0.834, respectively, at thresholds of 0.017 and 4 inds/m² for HSV and individuals per unit area. This meant that the HABITAT model accurately predicted the appearance of the benthic macroinvertebrates by approximately 77% and that the probability of false alarms was also very low. As a result of evaluating the suitability of habitats, in the Yeongsan river, if the annual "lowest level" (Seungchon weir: 2.5 EL.m/ Juksan weir: -1.35 EL.m) was maintained, the average habitat improvement effect of 6.5%P compared to the 'reference' scenario was predicted. Consequently, it was demonstrated that the integrated modelling framework for habitat suitability assessment is able to support the remedy aquatic ecological management.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.211-211
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• 2015

Mekong River is the world's $10^{th}$ longest river and runs through China's Yunnan province, Burma, Thailand, Laos, Cambodia and Vietnam. And Tonle Sap Lake, the largest fresh water body in Southeast Asia and the heart of Mekong River system, covers an area $2,500-3,000Km^2$ in dry season and $10,000-16,000Km^2$ in wet season. As previously noted, the water within Sap river flows from the Mekong River to Tonle Sap Lake in flood season (between June and October) and backward to Mekong River in dry season. Recently the flow regime of Sap River might be significantly affected by the development of large dams in upstream region of Mekong River. This paper aims at basic study about the large scale flood inundation of Cambodia using by CAESAR-Lisflood. CAESAR-Lisflood is a geomorphologic / Landscape evolution model that combines the Lisflood-FP 2d hydrodynamic flow model (Bates et al, 2010) with the CAESAR geomorphic model to simulate flow hydrograph and erosion/deposition in river catchments and reaches over time scales from hours to 1000's of years. This model is based on the simplified full Saint-Venant Equation so that it can simulate the interacted flow of between Mekong River and Tonle Sap Lake especially focusing on the flow direction change of Sap River by season.

• Journal of Magnetics
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• v.21 no.3
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• pp.431-436
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• 2016

In the design of transformer winding, natural vibration frequency is an important parameter. This paper presents a 2D model to calculate axial vibration natural frequency of power transformer winding based on the elastic dynamics theory, and according to the elastic support equivalent principle of radial pressboards. The 3D model to calculate natural vibration frequency can be simplified as a 2D one as the support of pressboards on the winding is same. It is verified that results of the 2D model are consistent with those of 3D one, but the former can achieve much higher calculation efficiency. It shows that increasing the width and number of pressboards can improve axial natural frequency through formula analysis and simulation, and also the relations between the changes of axial pre-compression and axial natural vibration frequency on the windings are investigated. Finally, the proposed 2D model's effectiveness is proved when compared with tested ones.