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The distribution and antimicrobial susceptibility of pathogenic microorganisms isolated from chicken slaughtering and processing procedure (닭 도계 및 가공공정 중 유해미생물의 분포와 항생제 감수성)

  • Seol, Kuk-Hwan;Kim, Ki Hyun;Jo, Su-Mi;Kim, Young Hwa;Kim, Hyun-Wook;Ham, Jun-Sang
    • Korean Journal of Agricultural Science
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    • v.42 no.1
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    • pp.29-35
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    • 2015
  • This study was performed to analyze the distribution and antimicrobial resistance of pathogenic microorganisms isolated from the carcass and environments of chicken processing plant located in Gyeonggi province from October to November in 2010. Chicken slaughterhouse was visited 3 times and totally 40 samples were collected from chicken carcass before and after washing (n=14), chicken cuts (n=7), cooling water (n=8), brine (n=2), cutting knives (n=7) and working plate (n=2). Whole-chicken rinsing technique (for chicken carcasses) and swab technique (for working plate and knives) were used to analyze the distribution of pathogenic microorganisms. In addition, brine and chilling water from storage tanks were gathered using sterilized tubes and used as samples. The matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) for whole cell fingerprinting in combination with a dedicated bioinformatic software tool was used to identify the isolated microorganisms. The pathogenic microorganisms, such as Bacillus cereus (n=8) and Staphylococcus aureus (n=9), were isolated form the chicken processing process (chicken carcasses of before and after chilling, chicken cuts, and working plate). The antimicrobial susceptibility of those isolated microorganisms was analyzed using 21 antimicrobial agents. In the case of B. cereus, it showed 100% of resistance to subclasses of penicillins and peptides, and it also resistant to cephalothin, a member of critically important antimicrobials (CIA), however there was no resistance (100% susceptible) to vancomycin and chloramphenicol. S. aureus showed 100% resistance to subclasses of peptides and some of penicillins (penicillin and oxacillin), however, it showed 100% susceptibility to cephalosporins (cefazolin and cephalothin). All of the tested pathogens showed multi drug resistance (MDR) more than 4 subclasses and one of B. cereus and S. aureus showed resistance to 9 subclasses. After the ban on using the antimicrobials in animal feed in July 2011, there would be some change in microbial distribution and antimicrobial resistance, and it still has a need to be analyzed.

Study on Softness of an EO Adducted Amine Oxide Zwitterionic Surfactant (EO가 부가된 아민 옥사이드 양쪽성 계면활성제의 유연력에 관한 연구)

  • Kim, JiSung;Mo, DaHee;Lee, JinSun;Park, JunSeok;Han, DongSung;Lim, JongChoo
    • Korean Chemical Engineering Research
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    • v.50 no.6
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    • pp.960-968
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    • 2012
  • In this study, the measurement of physical properties of 3 different ethylene oxide adducted zwitterionic surfactants were measured such as critical micelle concentration, surface tension, interfacial tension, contact angle, viscosity and foam stability. Also, the dual function characteristics of the zwitterionic surfactants were investigated by determining an isoelectric point, which were obtained using zeta potential measurement and QCM (quartz crystal microbalance) experiments. The isoelectric point of the synthesized zwitterionic surfactant determined by zeta potential measurement was close to that obtained by QCM experiment and both results have shown almost the same trend as that determined by the frictional property measured using an automated mildness tester. In particular, it has been observed that all three surfactants used during this study provide better softening effect at a pH of neutral condition than at an acidic or an alkaline condition. This result indicates that the synthesized surfactants act as a cationic surfactant at a pH of neutral condition and thus provide good softening effect during a rinsing cycle in the detergency process.

PRESENT STATE AND PROSPECT OF PUBLIC DENTAL HEALTH SERVICE FOR CHILDREN AND ADOLESCENTS IN KOREA (우리나라 어린이와 청소년의 구강건강을 위한 공공서비스의 현황과 전망)

  • Lee, Kwang-Hee;Kim, Jee-Young;Song, Ji-Hyon;Kim, Yun-Hee;Im, Kyeong-Uk;Jeong, Seung-Yeol
    • Journal of the korean academy of Pediatric Dentistry
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    • v.35 no.3
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    • pp.578-588
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    • 2008
  • To achieve the dental health of children and adolescents which is the objective of the pediatric dentistry, the role of the public service should be increased. The basis of the public service is established by many laws of which the Dental Health Law is most important. The percentages of primary schools that had the school dental health clinic, that implemented the fluoride rinsing program, and that implemented the group toothbrushing after lunch were 7.2%, 57.5%, and 46.9%, respectively, and the percentages of primary school children that received the dental health education and that received the fissure sealing were 48.0% and 12.4%, respectively(2006). About 42% of infants and preschool children from 0 to 6 years received preventive dental care in the last one year, at the nursery or kindergarten(18%) or at the health center(1%)(2005). The percentage of the health centers that implemented water fluoridation was 11.3%, and the percentage of the population who drank the fluoridated water was 5.7%(2006). It was suggested that the school dental health administration should be unified, that dental health teachers should be employed, and that the comprehensive dental health care should be supplied to all the children and adolescents through the circuit school dentists and the school dental hospitals in the long term. Also, the dentist in charge system for the children and adolescent was suggested.

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Factors affecting usage of toothpaste in infants and preschoolers (영유아의 세치제 사용에 영향을 미치는 요인)

  • Choi, Min-Ji;Park, Ji-Young;Lim, Mi-Young;Shuai, Jianfei;Heo, Jung;Jung, Da-Young;Ryu, Hyeon-Su;Oh, Na-Rae;Kim, Sun-Shin;Yang, Won-Ho
    • Journal of Korean society of Dental Hygiene
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    • v.17 no.1
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    • pp.49-62
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    • 2017
  • Objectives: This study was conducted to suggest the necessity of education about toothpaste usage to infants, preschoolers, and their parents. Also, another purpose is to provide basic data that is necessary for oral health education about accurate cognition and usage of toothpaste. Methods: From October to December 2015, we conducted a survey on parents of 0~4 years old infants and preschoolers about toothpaste usage based on the data of a questionnaire with a total of 505 subjects. The survey questions were composed of general characteristics of infants, preschoolers and their parents, and toothpaste usage. Results: Usage frequency of toothpaste for infants and preschoolers increased with age (p<0.001), and infants and preschoolers in homes with monthly income under 4 million won showed high frequency of toothpaste usage (p<0.001). Toothpaste mouth rinsing of infants and preschoolers increased with age (p<0.001), while on the other hand, toothpaste swallowing decreased (p<0.001). Conclusions: In this study, it was shown that the factors that effect toothpaste usage in infants and preschoolers were age and average monthly income of the family. This suggests the necessity of oral health education to parents about usage of toothpaste in infants and preschoolers.

A Study on the Actual Condition of Dental Prosthesis of the Elderly in Seoul and Gyeonggi-do (서울 및 경기지역 노인의 치과보철물 실태에 대한 조사 연구)

  • Lee, Tae-Jung;Jung, Jung-Ock;Lee, Kyeong-Hee
    • Journal of Technologic Dentistry
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    • v.33 no.4
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    • pp.369-378
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    • 2011
  • Purpose: This study was carried out for the purpose of obtaining basic data necessary for developing the future oral health promotion program of the elderly by surveying the actual condition of managing dental prosthesis in the elderly. Methods: It collected materials targeting 346 elderly brackets in over 65 years old who dwell in Seoul and Gyeonggi-do Province for about 10 months from September 2010 to June 2011, analyzed 312 copies except 34 copies, which are inappropriate for data processing due to being insufficient in response among these things, and obtained the following results. Results: In the actual condition of missing tooth, a case of missing tooth was indicated to be 78.8%. In case of missing tooth, the number of missing teeth was indicated to be 75.6% for under 5 pieces and 19.1% for over 21 pieces. In the actual condition of managing prosthesis, a case with dental prosthesis treatment was indicated to be 77.6%. A kind of prosthesis was indicated to be the highest in fixed partial dentures with 36.5%. In a method of rinsing dentures, 47.7% of the elderly responded as saying of washing it out with water. The appearance of removing dentures at night was indicated to be the highest in doing so with 63.1%. Satisfaction with prosthesis of being mounted now was indicated to be moderate with 50.4%. As for the appearance of having experience of education on how to manage prosthesis, it was indicated to be high with 50.4% in having none. In a kind of prosthesis depending on averagely monthly income, the ratio of total dentures was high when averagely monthly income is low. On the other hand, a case with high income was indicated to have high ratio in implants. The satisfaction with prosthesis was low when averagely monthly income is low. Also, in the experience of education on how to manage prosthesis, the ratio of responses as saying of having no experience of education on how to manage prosthesis was indicated to be high when averagely monthly income is low. Conclusion: Examining the above results, a developmental plan is considered to be necessary such as developing continuous and systematic oral health promotion program for managing elderly people's managing dental prothesis.

THE ADHESIVE PATTERNS OF COMPOMER TO SALIVA-CONTAMINATED DENTIN (타액에 오염된 상아질에 대한 콤포머의 접합양상)

  • Cho, Young-Gon;Kim, Byung-Tae;Lee, Suk-Jong
    • Restorative Dentistry and Endodontics
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    • v.25 no.4
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    • pp.575-586
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    • 2000
  • In this study, adaptation of compomer to saliva contaminated dentin was evaluated with scanning electron microscope(SEM) and confocal laser scanning microscope(CLSM). For the SEM study, the occulusal surfaces of thirty two molar teeth were grounded to exposure dentin surfaces. The specimen were randomly assigned to control and three experimental groups with four samples in each group. In control group, Dyract and F-2000 compomer were bonded on the specimens according to the manufactures direction. Experimental groups were subdivided into three groups. They were contaminated with saliva on dentin surfaces ; Experimental group 1 : Saliva was dried with compressed air. Experimental group 2 : Saliva was rinsed with air-water spray and dried. Experimental group 3 : After polymerization of an adhesive, they were contaminated with saliva, and then saliva was rinsed with air-water spray and dried. Dyract and F-2000 compomer were bonded on saliva-treated dentin surfaces. The interfaces between dentin and compomer were observed with SEM. For the CLSM study, Class V cavities were prepared in buccal and ligual surfacess of thirty two molars. The specimens were divided into control and experimental groups. Class V cavities in experimental group were contaminated with saliva and those surfaces in each experimental groups received the same treatments as for the SEM study. Cavities were applied Prime & Bond 2.1 and F-2000 compomer primer/adhesive that were mixed with fluorescein, and then were filled with Dyract and F-2000 compomer. Specimens were embedded in transparent acrylic resin and sectioned buccolingual1y with diamond wheel saw, and then mounted on cover slide for CLSM study. The interface between cavity and compomer was observed by fluoresence imaging with a CLSM. The results were as follows : 1. In SEM exammination of Dyract group, control group, experimental group 2, 3 showed close adaptation to dentin and hybrid layer of $3{\sim}4{\mu}m$ diameter. Interfacial gap between compomer and dentin in experimental group 1 was wider than in control group. 2. In SEM examination of F-2000 group, adaptation to dentin of control group was closer than Dytact control group, but hybrid-like layer was not observed. Interfacial gap between compomer and dentin in experimental group 1 was wider than in Dyract experimental group 1. 3. In dissolution specimens of Dyract and F-2000 group, resin tags penetrated through dentinal tubules in control group and experimental group 1 and 3, but the penetration of resin tag was irregular and partial in experimental group 1. 4. In CLSM exammination of Dyract and F-2000 group, adhesive patterns of control and experimental groups showed same as in SEM. This result suggests the treatment methods, rinsing & drying, repeating all adhesive procedures, will produce good effect on adaptation of compomer to dentin if the dentin surface or polymerized adhesive is contaminated by saliva.

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A Study on the Removal of Deposited Calcium on the Cotton Fabric. (직물에 침착된 Calcium의 제거에 관한 연구)

  • Han Hae Won;Kahng He Won;Kim Sung Reon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.7 no.2
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    • pp.19-25
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    • 1983
  • The purpose of this study was to investigate the effects of laundry variables and additives on the removal of deposited calcium on the cotton fabric. Samples of calcium deposited fabric was made by treating fabric with $CaC1_2$ and $Na_2CO_3$ solution subsequently. The experimental variables were: 1) NaOH concentration ($0.0001\%$, $0.0005\%$, $0.001\%$, $0.005\%$, $0.01\%$) 2) Alkaline builders(sodium carbonate, sodium meta silicate) 3) Sequestering agents(STPP and EDTA concentration: $0.02\%$, $0.04\%$, $0.06\%$, $0.08\%$, $0.1\%$, $0.15\%$, $0.2\%$) 4) Temperatures($25\pm1^{\circ}C$, $40\pm1^{\circ}C$, $60\pm1^{\circ}C$) 5) Edge-abrasion to the removal of deposited calcium on the cotton fabric. The fabric was washed for 15 minutes in a washing machine(Model: Gold Star WP-3007) or Launder-0-meter(40$\~$45 r.p.m., Toyo Rika Instrument Inc.) and rinsed 3 times per every rinsing time. The amount of calcium deposits on the fabrics was determined by EDTA-back titration methods and edge-abrasion was evaluated by ASTM D 3886 method. The results of this study were as follows: 1) pH of surfactant solution(NaOH concentration) did not influence on the removal of deposited calcium on the cotton fabric. 2) Added alkaline builders did not influence on the removal of deposited calcium on the cotton fabric. 3) It was shown that STPP and EDTA were effective to remove deposited calcium. The removal of deposited calcium on the cotton fabric was proportionally increased with increasing concentration of STPP and EDTA. At high concentration, however, the rate was rather decreased with increasing concentration. 4) The temperature of washing solution did not influence on the removal of dedosited calcium on the cotton fabric. 5) As the removal of deposited calcium on the cotton fabric was increased, the rate of edge-abrasion of the fabric was gradually increased.

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A Study on the Mill Scale Pretreatment and Magnetite Production for Phosphate Adsorption (인 흡착을 위한 Mill Scale 전처리 및 Magnetite 제조 연구)

  • Chun, Hyuncheol;Choi, Younggyun
    • Journal of Korean Society of Environmental Engineers
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    • v.37 no.4
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    • pp.246-252
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    • 2015
  • In steel factory, hot roller cleaning process produces a lot of iron oxide particles called as mill scale. Major components of these particles are wustite (FeO), magnetite ($Fe_3O_4$), and hematite ($Fe_2O_3$). In this study, we tried to produce pure magnetite from the mill scale because of the largest phosphate adsorption capacity of the magnetite. The mill scale was treated with acid (HCl+$H_2O_2$), base (NaOH), and acid-base ($H_2SO_4$+NaOH). Batch adsorption tests showed the acid and/or base treatment could increase the phosphate adsorption capacity of the iron oxides from 0.28 to over 3.11 mgP/g. Magnetite, which could be obtained by acid and base treatment of the mill scale, showed the best adsorption capacity. From the kinetic analysis, both Freundlich and Langmuir isotherm well described the phosphate adsorption behavior of the magnetite. In Langmuir model, maximum phosphate adsorption capacity was found to be 5.1 mgP/g at $20^{\circ}C$.

Survey on the Changes of Potassium and Magnesium Concentration according to the Retention Time After Rinse of Sucrose and Glucose in Oral (구강 내 자당 및 포도당 잔류 경과 시간에 따른 칼륨과 마그네슘 농도 변화)

  • Lee, Hye-Jin;Yang, Dal-Nim
    • The Journal of the Korea Contents Association
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    • v.19 no.11
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    • pp.424-433
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    • 2019
  • The purpose of this study was to evaluate the salivary concentration of Potassium and Magnesium cations and their variation before and after sucrose and glucose rinse, and to investigate the relationship between the levels of each compound. Saliva samples were obtained from 40 subjects before and up to 60 min after intake of a 10% sucrose and glucose solution at 1-month intervals. Potassium and Magnesium in human saliva were determined via anion-exchange chromatography with an anion-suppressed conductivity detector using 12 mM sulfuric acid. The concentrations of Potassium and Magnesium before sucrose rinse were 274.3±77.9 mg/ℓ and 4.5±2.5 mg/ℓ, also, the concentrations of Potassium and Magnesium before glucose rinse were 279.2±62.1 mg/ and 4.8±2.0 mg/ℓ, respectively. Potassium and Magnesium concentrations were significantly decreased (p<0.05) after sucrose rinse. The content of potassium and magnesium in saliva before and after rinsing sucrose and glucose is difficult to standardize or classify, as previous research. The reason for the variation between individuals is large, and easily changed by chemical or physiological stimulation. However, this study was experiment for the purpose of accumulating basic data for saliva.

Removal of Malathion Residues from Fruits and Vegetables by Washing Processes (과일채소중 말라티온 잔류분(殘溜分)의 세척효과)

  • Shim, Aei-Ryun;Choi, Eon-Ho;Lee, Su-Rae
    • Korean Journal of Food Science and Technology
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    • v.16 no.4
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    • pp.418-422
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    • 1984
  • In order to determine the removal efficiency of pesticide residues in fruits and vegetables by washing processes, samples of lettuce, young Chinese radish, Chinese cabbage, green red pepper, strawberry and grape were artificially contaminated with malathion and washed according to the household practices. After three times washings with water, the remaining ratios of malathion residues in grape, green red pepper, strawberry, young Chinese radish, lettuce and Chinese cabbage were 9.7%, 25.2%, 28.0%, 29.7%, 38.9% and 57.5%, respectively. After washing with detergent solution followed by two times rinsing with water, the remaining ratios of malathion residues in the same food samples were 1.6%, 8.3%, 15.8%, 24.8%, 27.2% and 45.9%, respectively. The removal efficiency of malathion by detergent washing was significantly higher than the water washings only. The removal ratio of malathion residues was the highest in the first washing and the ratio fell greatly in the following washings. The removal efficiency was not quite different in the temperature range of $5-35^{\circ}C$ of the washing solution, but the efficiency at $100^{\circ}C$ was 2-3 times higher than other temperature ranges.

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