• 한국식품위생안전성학회지
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• 제34권3호
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• pp.263-268
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• 2019

메탄올은 부동액, 세제, 살균제, 소독제 및 공업용매 등 다양한 분야에서 사용되는 독성 알코올이며, 섭취, 경피흡수 및 흡입을 통해 인체에 노출된다. 체내에서 메탄올은 포름알데히드와 포름산으로 산화되며, 이러한 생성물을 대사산증, 시신경장애를 일으키고, 심한 경우 사망에 이르기도 한다. 본 연구에서는 국내에서 유통되는 세척제 및 헹굼보조제 중 메탄올의 함량을 모니터링하고자 하였으며, 기존의 식약처에서 고시한 방법보다 더 간단하고 빠른 GC-HS-MS방법으로 진행하였다. 본 분석법은SIM 모드로 진행하였고(메탄올 31 m/z 및 이소프로판올 45 m/z), LOD는 1.09 mg/kg으로 계산되었으며, 정확도와 정밀도는 각각 91.1-97.9%와 10%이하로 나타나 정량분석에 적합한 수준임을 확인하였다. 본 연구는 세척제 중 메탄올 함량을 모니터링한 최초의 연구로써 가치가 있다고 판단된다.

• 분석과학
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• 제33권3호
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• pp.125-133
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• 2020

Methylisothiazolinone (MIT) and chloromethylisothiazolinone (CMIT) cause allergic contact dermatitis and are banned cosmetics ingredients, except in rinse-off products. However, their presence has been detected in cosmetics. We report a UPLC-tandem MS/MS screening method for their simultaneous determination in cosmetics. To facilitate extraction from various matrices, pretreatment methods were developed for each sample type. The method was optimized through a series of assessments, including specificity, LOD, LOQ, linearity, recovery, stability, precision, and accuracy. The LODs and LOQs for MIT ranged from 0.054 and 0.163 ㎍ mL^-1 whereas those for CMIT ranged from 0.040 and 0.119 ㎍ mL^-1. The linear correlation coefficients (r²) were higher than 0.999. Relative standard deviations (RSDs) for both intra- and inter-day measurements ranged from 0.3 ~ 13.6 %. Recoveries at three different concentrations were within 87.9 ~ 118.9 %. The RSD for stability measurements of spiked samples was within 7 %. These results confirm the suitability of the developed method for the simultaneous quantitation of MIT and CMIT in cosmetics. Samples of 320 color cosmetics, including eyeshadows, solid lipsticks, liquid lipsticks, and nail polishes were analyzed using the developed method, and two of them were found to contain both MIT and CMIT and one of them was found to contain only MIT. This data and the method will aid the regulation of ingredients used in cosmetics.