• Title/Summary/Keyword: Ricinus communis

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Antimicrobial and Antioxidant Activity of Hot Water and Ethanol Extracts of Ricinus communis L. Leaves and Fruits (피마자(Ricinus communis L.) 잎과 열매의 열수(熱水) 및 에탄올 추출물의 항균 활성과 항산화 효능)

  • Park, Jang-Soon
    • Journal of Digital Convergence
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    • v.16 no.6
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    • pp.353-361
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    • 2018
  • RFW and RFE of castor fruit selected as part of the development of natural antimicrobials and antioxidants yielded 15.8% and 18.4% respectively. In the results of measuring the antimicrobial activity through paper disc method, the antimicrobial activity of castor fruits in ethanol extracts appeared. Especially, the activity was excellent in P. aeruginosa and S. aureus, and antimicrobial activity of C. was 1.5mm up to 16 hours. However, the proliferation of C. was observed again after 24 hours. In the MIC experiment results of RFE, S. aureus and P. aeruginosa showed 96% and 93% of antimicrobial activity, respectively. The DPPH radical scavenging activity of RLW and castor leaf ethanol extract showed $1.8{\pm}0.6%$ and $2.1{\pm}0.7%$ free radical scavenging activity at $1000({\mu}g/m{\ell})$. This study is expected to provide basic data for the development of antimicrobial agents and antioxidants using natural products.

Single and mixed chelants-assisted phytoextraction of heavy metals in municipal waste dump soil by castor

  • Wuana, Raymond A.;Eneji, Ishaq S.;Naku, Julius U.
    • Advances in environmental research
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    • v.5 no.1
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    • pp.19-35
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    • 2016
  • The phytoextraction of some toxic heavy metals from municipal waste dump soil by castor plant (Ricinus communis) was tested under natural and single or mixed chelant-assisted scenarios in pot microcosms. A sandy loam with total metal contents (mg/kg): Cd (84.5), Cu (114.5), Ni (70.3), Pb (57.8), and Zn (117.5), was sampled from an active dumpsite in Calabar, Nigeria and used for the study. Castor (small seed variety) was grown under natural phytoextraction or single/binary chelant (citric acid, oxalic acid, and EDTA) applications (5-20 mmol/kg soil) for 63 days. Castor exhibited no visual phytotoxic symptoms with typically sigmoid growth profiles at the applied chelant doses. Growth rates, however, decelerated with increase in chelant dose. Post-harvest biomass yields were higher under chelant application than for natural phytoextraction. Both root and shoot metal concentrations (mg/kg) increased quasilinearly and significantly ($p{\leq}0.05$) with increase in chelant dose, furnishing maximum levels as: Cd (55.6 and 20.9), Cu (89.5 and 58.4), Ni (49.8 and 19.6), Pb (32.1 and 12.1), and Zn (99.5 and 46.6). Ranges of translocation factors, root and shoot bioaccumulation factors were 0.21-3.49, 0.01-0.89 and 0.01-0.51, respectively. Overall, the binary chelant treatments were less toxic for R. communis growth and enhanced metal accumulation in shoots to a greater extent than the single chelant scenarios, but more so when EDTA was present in the binary combination. This suggests that the mixed chelants could be considered as alternative treatments for enhanced phytoextraction and revegetation of degraded waste dump soils.

Analysis of Crude Fat and Fatty Acid in Collections of Ricinus communis L. (아주까리(비마자) 수집종의 조지방 및 지방산 분석)

  • Kim, In-Jae;Nam, Sang-Young;Kim, Min-Ja;Rho, Chang-Woo;Yun, Tae;Kim, Hong-Sig;Song, Hang-Lin;Heon-Sang, Jeong
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.5
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    • pp.301-305
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    • 2008
  • Forty Ricinus communis collections were obtained from RDA National Agrobiodiversity Center for knowing the possibility of the use as a bio-diesel possibility crop. These are analysis results about crude fat and fatty acid. Gas chromatogram of seed collections analysis showed 6 peaks and retention time of ricinoleic acid was about 17.1 minute. Average oil content of collections were ranged from 44.6 to 49.4% and the difference was between maximum 52.5% and minimum 41.4%. Fatty acid composition was almost unsaturated fatty acid of 97.6% and saturated fatty acid showed low content of 2.4%. Ricinoleic acid was 87.3% and the content of oleic acid and linoleic acid in fatty acid was 4.6%, and 5.2%, respectively. The content of palmitic acid and stearic acid was about 1% and the difference was insignificant. The content of linolenic acid was extremely low as 0.6%.

UV-spectrophotometric and electrophoretic analysis composition of protein and the content of sugars of young Ricinus communis L. by cold treatment (UV-분광광도법과 전기영동에 의한 피마자 유식물에서 저온처리에 의한 당류 함량과 단백질 구성의 분석)

  • Cho, Bong-Heuy;Park, Sun Young;Park, Myon-Yong
    • Analytical Science and Technology
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    • v.8 no.2
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    • pp.181-186
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    • 1995
  • The contents of sucrose and reducing sugars of cotyledons and hypocotyls of Ricinus communis L were increased slightly during cold treatment at $4^{\circ}C$. The concentration of total amino acids was increased continuosly during cold treatment. But the contents of hydrophilic amino acids, Asp/Asn, Glu/Gln, Thr, Ser, Ala and cationic amino acids, Arg and Lys were varied dramatically with the cold treated time. The cold treatment induced 24, 52, 54, 55, 56 and 73.5kD of proteins in cotyledons and 55, 56 and 73.5kD of proteins in hypocotyls. 24, 42, 49 and 52kD of proteins in cotyledons and hypocotyls were boiling stable. They were not denatrated by boiling at $100^{\circ}C$.

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Synthetic method and insecticidal activity of ricinine (Ricinine의 합성법 및 살충활성)

  • Kwon, Oh-Kyung;Lim, Soo-Kil;Choi, Dal-Soon;Kyung, Suk-Hun
    • The Korean Journal of Pesticide Science
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    • v.2 no.1
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    • pp.18-23
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    • 1998
  • In order to obtain leading compound for the development of new pesticide through the organic synthesis of natural products, the synthesis of ricinine, an active compound of Ricinus communis, was established and biological activities of synthetic compounds against insects were examined. The synthetic scheme of ricinine was composed of four steps by the spontaneous condensation of the cyanoacetyl chloride. A modified synthetic process was also estabilshed to enhance the synthetic yield by simple cyclization of ethoxymethylene malononitrile. In the bioassay results of synthetic ricinine and intermediates on four insects, the mortality of ricinine on brown planthopper (BPH, Nilaparvata lugens) and pea weevil(PW, Bruchus rufimanus) was 80% and 75% at the concentration of 1,000 ${\mu}g/ml$ respectively. Chloronorricinine and chlororicinic acid having chloride group in molecular structure gave 60% mortality on two-spotted mite (TSSM, Tetranychus urticae) at the concentration of 500 ${\mu}g/ml$. The mortality of compounds on house mosquito (HM, Culex pipens pallens) was meager at 10 ${\mu}g/ml$ level.

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Electrophoretic Analysis of Cotyledons and Roots of Ricinius communis L. by Abscisic Acid(ABA) Treatment (전기영동에 의한 피마자 자엽과 뿌리에서 Abscisic acid(ABA) 처리에 의한 단백질의 분석)

  • Cho, Bong-Heuy;Park, Sun Young;Lee, Jong Ho
    • Analytical Science and Technology
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    • v.9 no.1
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    • pp.13-19
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    • 1996
  • To treatment of Ricinus cotyledons with ABA, induced several proteins with molecular weights of 53, 54, 56, 58 and 73.5kD. 54 and 56kD among those proteins resulted in increased more when ABA concentrations in external media are increased. The molecular weight of 35, 49, 53, 54, 62, 65 and 79kD of proteins are induced by ABA treatment of roots. The induced proteins are not the same a those by cold treatment exception of 73.5kD of cotyledons and 62kD of roots. 49, 58 and 79kD of proteins are important to research in future because of the induction of proteins in the presence of cydoheximide(CH) which is blocked the synthesis of proteins.

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Histological and Histochemical Study on Toxicity of Ricin in the Rat Liver (흰쥐 간에 미치는 Ricin의 독성에 대한 조직학적 및 조직화학적 연구)

  • 조운복;최병태
    • Journal of Environmental Science International
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    • v.6 no.1
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    • pp.53-60
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    • 1997
  • The toxicity of purified ricln from Ricinus communis to rats was examined by histological and histochemical methods. Sprague-Dawley rats were injected intraperitoneally with 75$\mu\textrm{g}$/kg body weight of ricin and were sacrified at itntervals of 6, 24, 48 and 120 hours after injectoon. The major morphological changes, such as cloudy swelling, hydropic degeneration, necrosis, fatty change, blood congestion, increase of Kupffer cells in number and extension of sinusoids, were obvious in the liver of experimental group. These morphological changes of hepatic cells were mainly observed in both the periportal and midlobular region of hepatic lobule. The extension of sinusoids was obvious in the controlobular region. And glycogen dlstrlbution of hepatic cells tended to decrease in the same region showing morphlogical changes as compared with the control group.

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Identification of Species-Specific Components between Hanwoo and Holstein Meat (한우 및 홀스타인육의 품종간 특이성분의 검색에 관한 연구)

  • 황보식;이수원;임태진;정구용
    • Food Science of Animal Resources
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    • v.21 no.3
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    • pp.246-255
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    • 2001
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of muscles extracted with distilled water, saline solution, SDS or Trition X-100 showed simular protein patterns between Hanwoo and Holstein meat, indicating that SDS-PAGE technique may not be useful for the identification between Hanwoo and Holstein meat. Lectine blot analysis of muscle extracted with distilled water demonstrated that Hanwoo and Holstein meat had similar affinities for concanavalin A (Con A), ricinus communis agglutinin (RCA-120), ulex europaeus agglutinin (UEA-1) or peanut agglutinin (PNA) lectins. However, approximately 32.1 kDa component of Hanwoo meat showed high affinity for dolichos biflorus agglutinin (DBA) lectin. On the contrary, high molecular weight components of Holstein meat had the specific affinity for wheat germ agglutinin (WGA) lectin. Hanwoo meat-specific components were observed by lectin staining of heat-denatured meat at 100$^{\circ}C$ for 30 sec. Also, the component of heat-denatured meat at 100$^{\circ}C$ for 30 sec, which was slightly smaller than Hanwoo meat-specific component, was concentrated specifically in Holstein meat.

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