• Journal of Animal Science and Technology
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• v.48 no.5
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• pp.691-698
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• 2006

Three species of the yeast Saccharomyces cerevisiae, Humicola grisea and Candida glabrata were assumed as microbial inoculants for fermentation of rice straw silage. Four types of silage innoculated with three yeasts including control (non-treatment) were opened on day 1, 3, 6, 9, 15 and 20 after ensiling, and analyzed for fermentation status (pH, crude protein, microbial counts) and the microbial population attached with silage texture using SEM (Scanning Electron Microscopy). The results obtained were summarized as fallow; The pH of silage juice was decreased to 4.3 after 6th day of fermentation in the treatments innoculated with yeast, but was not changed at the ranges of 5.47 to 5.67 in control. Crude protein concentration of silage was increased by 38～41% with yeast inoculation compared to control. From SEM observation, it could be confirmed that crude protein concentration of silage was increased by microbial growth and SCP synthesis. The yeast Saccharomyces cerevisiae and Candida glabrata could be used as useful fermenters of rice straw silage.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.2
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• pp.180-187
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• 2015

This study was conducted to determine the effects of feeding by-product feed (BF)-based silage on the performance, blood metabolite parameters, and carcass characteristics of Hanwoo steers. The BF-based silage was composed of 50% spent mushroom substrate, 21% recycled poultry bedding, 15% cut ryegrass straw, 10.8% rice bran, 2% molasses, 0.6% bentonite, and 0.6% microbial additive (on a wet basis), and ensiled for over 5 d. Fifteen steers were allocated to three diets during the growing and fattening periods (3.1 and 9.8 months, respectively): a control diet (concentrate mix and free access to rice straw), a 50% BF-based silage diet (control diet+50% of maximum BF-based silage intake), and a 100% BF-based silage diet (the same amount of concentrate mix and ad libitum BF-based silage). The BF-based silage was fed during the growing and fattening periods, and was replaced with larger particles of rice straw during the finishing period. After 19.6 months of the whole period all the steers were slaughtered. Compared with feeding rice straw, feeding BF-based silage tended (p = 0.10) to increase the average daily gain (27%) and feed efficiency (18%) of the growing steers, caused by increased voluntary feed intake. Feeding BF-based silage had little effect on serum constituents, electrolytes, enzymes, or the blood cell profiles of fattening steers, except for low serum Ca and high blood urea concentrations (p<0.05). Feeding BF-based silage did not affect cold carcass weight, yield traits such as back fat thickness, longissimus muscle area, yield index or yield grade, or quality traits such as meat color, fat color, texture, maturity, marbling score, or quality grade. However, it improved good quality grade (1+ and 1++) appearance rates (60% for the control group vs 100% for the BF-based silage-fed groups). In conclusion, cheap BF-based silage could be successfully used as a good quality roughage source for beef cattle.

• Journal of The Korean Society of Grassland and Forage Science
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• v.4 no.1
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• pp.23-27
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• 1983

This experiment was carried out to show how to make the silage of raddish and Chinese cabbage leaves remained after pickling vegetable in autumn. The moisture of raw materials was controlled by the rice straw and barley bran. The results obtaind were as follows; 1. the silage composed of vegetable leaves 70%, rice straw 20% and barley bran 10% showed the best quality and desirable acid content. 2. Adding barley bran increased the crude protein, crude fat, and crude fiber in the silage. 3. 10% of barley bran made the silage more palatable and also have a good colour.

• Proceedings of the Korean Society of Grassland Science Conference
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• 2005.06a
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• pp.184-185
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• 2005

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.3
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• pp.207-214
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• 1995

'Ihis experiment was canied out to investigate the feed intake, chewing activities and ruminal characteristics by change in roughage intake and concentrate feeding amount with 15 Holstein cows. 1. DM intake of cow fed rice straw, corn silage and grass hay was 4.97, 5.47 and 5.60% of metabolic weight, respectively, and that by kg increase of concentrate was decreased by 0.330, 0.294 and 0.461 kg, respectively. 2. RVI of rice straw, com silage and grass hay was 98.3, 93.7 and 87.1 respectively, and correlations between RVIfl) and concentrate level@) were the following; $Y_1$(rice straw) = 99.4543 - 5.9759X + 0.0230$X^2$ This experiment was carried out to investigate the feed intake, chewing activities and ruminal characteristics by change in roughage intake and concentrate feeding amount with 15 Holstein cows. 1. DM intake of cow fed rice straw, corn silage and grass hay was 4.97, 5.47 and 5.60% of metabolic weight, respectively, and that by kg increase of concentrate was decreased by 0.330, 0.294 and 0.461 kg, respectively. 2. RVI of rice straw, com silage and grass hay was 98.3, 93.7 and 87.1 respectively, and correlations between RVI(Y) and concentrate level(X) were the following; $Y_1$(rice straw) = 99.4543 - 5.9759X + 0.0230$X^2$ $Y_2$(com silage) = 88.7601 - 2.9000X - 0.2111$X^2$ $Y_3$(grass hay) = 95.1515 - 6.3096X + 0.0873$X^2$ 3. Average ruminal pH in rice straw feeding group was the highest and correlations between ruminal pH(Y) and concentrate level(X) were the following; $Y_1$ = 7.0920 - 0.0309X - 0.0023$X^2$ $Y_2$ = 6.5000 - 0.0516X + 0.0002$X^2$ $Y_3$= 6.7666 - 0.0072X - 0.0044$X^2$ 4. Average ruminal $NH_3-N$ concentration in forage hay feeding group was the highest and correlations between ruminal $NH_3-N$ concentration(Y) and concentrate level(X) were the following. $Y_1$ = 3.3777 - 0.0587X + 0.0682$X^2$ $Y_2$ = 4.2765 + 0.5921X + 0.0181$X^2$ $Y_3$= 5.3634 - 0.4203X + 0.0223$X^2$ 5. Ruminal VFA wncentmtion in corn silage feeding group was the highest.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.1
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• pp.21-29
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• 2013

The studies were conducted to investigate real condition of mycotoxin contamination in the fields before harvest and by the storages of rice straw used as roughage in Korea. It was found mycotoxin contamination before harvest of rice straw that the rice plants were detected deoxynivalenol at the initial growth, ochratoxin A and deoxynivalenol at the middle growth, and deoxynivalenol and zearalenone at the harvest periods in the fields. Also, the rice plants were contaminated by various fungi such as Fusarium sp., Fusarium proliferatum, Penicillium sp., Gibberella sp., Gibberella zeae, Mucor circinelloides and Aspergillus oryzae. The levels of fungal contamination were $10^{3-4}$ cfu/g at the initial growth, and $10^{4-5}$ cfu/g at the middle growth and harvest periods. All storage types of rice straw were contaminated with zearalenone, deoxynivalenol and ochratoxins A. The samples of rice straw contaminating mycotoxins were account for 3% in bundle rice straw, and 38% in both types of square rice straw and rice round bale silage, respectively. When 105 samples of rice bale silage were analyzed for mycotoxins depending on the regional area, mycotoxin contamination was found in 46% of total samples in Korea. Regional contaminations of mycotoxins were respectively 48, 33, 40, 50 and 57% of samples in Gyeonggi, Gangwon, Chungcheng, Yeongnam and Honam area. Rice round bale silage was contaminated by three kinds of mycotoxins (zearalenone, deoxynivalenol and ochratoxinsA) in the all of area without Chungcheong area where was contaminated zearalenone and deoxynivalenol. Ochratoxins A, deoxynivalenol and zearalenone were respectively determinated with the average levels of 2.6, 413 and $338{\mu}g/kg$ in rice round bale silage for the overall area, even if it was some difference depending on each regional area. Therefore, the above results clearly show that the rice straws were exposed to the contamination by mycotoxin and mycotoxigenic fungi before harvest in the fields, and mycotoxin contamination was not dependent on the regional area or the storage types such as bundle rice straw, square rice straw and rice round bale silage.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.2
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• pp.181-186
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• 2017

Objective: Leucaena leucocephala (Leucaena) is a perennial tropical legume that can be directly grazed or harvested and offered to ruminants as hay, silage, or fresh. However, Leucaena contain phenolic compounds, which are considered anti-nutritional factors as these may reduce intake, digestibility and thus animal performance. Therefore, the objective of this experiment was to determine effects of Leucaena silage (LS) feeding levels on rumen microbial populations, N-balance and microbial protein synthesis in dairy steers. Methods: Four, rumen fistulated dairy steers with initial weight of $167{\pm}12kg$ were randomly assigned to receive dietary treatments according to a $4{\times}4$ Latin square design. Treatments were as followings: T1 = untreated rice straw (RS; Control), T2 = 70% RS+30% LS, T3 = 40% RS+60% LS, and T4 = 100% LS. Dairy steers were fed rice straw and LS ad libitum and supplemented with concentrate at 0.2% of body weight/d. Results: Results revealed that the rumen microbial population, especially cellulolytic, proteolytic bacteria and fungal zoospores were enhanced in steers that received 60% of LS (p<0.05), whereas the amylolytic bacteria population was not affected by treatments (p>0.05). Protozoal population was linearly decreased with increasing level of LS (p<0.05). Moreover, N-balance and microbial protein synthesis were enhanced by LS feeding (p<0.05) and were the highest in 60% LS group. Conclusion: Based on this study, it could be concluded that replacement of RS with 60% LS significantly improved microbial population and microbial protein synthesis in diary steers.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.2
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• pp.107-114
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• 2002

Rice straw silage added with apple pomace in different ratios were mixed with commercial concentrates and fed to Korean native goats in comparison to the corn silage mixed with the same content of commercial concentrates in whole diet as that of apple pomace added rice straw silage (rice straw : apple pomace = 100:0, 80:20, 60:40, 40:60). Dry matter intake, digestibilities of nutrients and nitrogen retention were investigated for the possibilities of application of agricultural by-products for the diets of Korean native goats. Crude protein contents of rice straw silage added with apple pomace were 6.3~7.3% and the contents of ADF, NDF and crude ash were highest in 100% rice straw mixed ratio (A) as 39.4, 61.6 and 8.9% respectively. Those were lower in corn silage (E) as 30.3, 53.4 and 4.9% respectively, however NSC content of corn silage was highest among the experimental treatments as 31.4%. Daily dry matter intakes per head and also per metabolic basal weight (DM g/kg of $BW^{0.75}$ were significantly (p<0.05) higher in D of the highest mixed ratio of apple pomace (605.3, 69.5g) than those of corn silage (E: 394.0, 46.8g). Daily live weight changes were significantly (p<0.05) higher in 40% (C: 16.7g) and 60% (D: 22.9g) apple pomace mixed ratios than 0% (A: 0.17g) and 20% (B: 4.3g) apple pomace mixed ratios. Digestibilities of dry matter and organic matter were higher in D and E than in A and B and those of ADF and NDF were higher in D as 50.2 and 57.4% respectively than the digestibilities in A, B and E. Nitrogen retention (g, %) was highest in D of the highest mixed apple pomace ratio (1.4g, 20.4%) however lowest in A (-0.3g, -7.75%).

• Animal Bioscience
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• v.34 no.6
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• pp.1038-1048
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• 2021

Objective: The study aimed to evaluate the effect of storage time and formic acid (FA) on fermentation characteristics, epiphytic microflora, carbohydrate components and in vitro digestibility of rice straw silage. Methods: Fresh rice straw was ensiled with four levels of FA (0%, 0.2%, 0.4%, and 0.6% of fresh weight) for 3, 6, 9, 15, 30, and 60 d. At each time point, the silos were opened and sampled for chemical and microbial analyses. Meanwhile, the fresh and 60-d ensiled rice straw were further subjected to in vitro analyses. Results: The results showed that 0.2% and 0.6% FA both produced well-preserved silages with low pH value and undetected butyric acid, whereas it was converse for 0.4% FA. The populations of enterobacteria, yeasts, moulds and aerobic bacteria were suppressed by 0.2% and 0.6% FA, resulting in lower dry matter loss, ammonia nitrogen and ethanol content (p<0.05). The increase of FA linearly (p<0.001) decreased neutral detergent fibre and hemicellulose, linearly (p<0.001) increased residual water soluble carbohydrate, glucose, fructose and xylose. The in vitro gas production of rice straw was decreased by ensilage but the initial gas production rate was increased, and further improved by FA application (p<0.05). No obvious difference of FA application on in vitro digestibility of dry matter, neutral detergent fibre, and acid detergent fibre was observed (p>0.05). Conclusion: The 0.2% FA application level promoted lactic acid fermentation while 0.6% FA restricted all microbial fermentation of rice straw silages. Rice straw ensiled with 0.2% FA or 0.6% FA improved its nutrient preservation without affecting digestion, with the 0.6% FA level best.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.1
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• pp.41-48
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• 1999

This experiment was carried out to determine the effect of the silage additives on improvement of quality of fresh rice straw silage using round bale at the forage experimental field, grassland and forage crops division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiment was arranged in a randomized block design with three replication. The treatments used in this study were consisted of different additives(control, formic acid, molasses, molasses+urea and inoculant). The rice straw silage with molasses+urea treatment resulted in high crude protein content and in vitro dry matter digestibility were increased with molasses of inoculant treatments compare with the control. The mean dry matter of formic acid treatment material was higher than with control but there was no significant difference in dry matter content among the additives treatments. The pH of molasses treatments significantly increased the proportion of lactic acid(P<0.05) and decreased the proportion of butyric acid. The total organic acid content of all treatments had low around 2%. Ammonia-N of molasses+urea treatment was significantly(P<0.05) higher than that of others, but formic acid or inoculant treatments was lower below 10% per total nitrogen. Over a 7d feeding period, the dry matter intake per cattle on the inoculant treatment was higher that on both the untreated round bale silage of fresh rice straw and rice straw hay. Producing cost per kilogram of round bale silage of fresh rice straw was decreased according to the increasement of harvesting area. It is suggested that application of round bale silage system to fresh rice straw with molasses or inoculant was the best treatment for improving preservation as silage, and that animal intake was enhanced by addition of inoculant to fresh rice straw.